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At least 271 records · Page 15Linked to original sources

Microwave methods of measuring changes in lung water.

Two microwave methods for measuring changes in lung water content have been investigated. The first is based on measuring changes in the phase of an active microwave signal transmitted through the thorax, while the other is based on measuring the natural microwave radiation emitted by the body with a radiometer. The feasibility of using the active microwave method is investigated by making measurements on phantoms, on anesthetized dogs with induced pulmonary edema, and on isolated dog lungs. The obtained results clearly illustrated that the method is sensitive enough to measure small changes in lung water content. In the radiometer method, on the other hand, both calculations and preliminary measurements in phantoms indicate that the natural microwave emission changes with lung water content and that conventional radiometry systems should be able to detect as little as three to four percent change in lung water content. Initial results from both methods, therefore, indicate the soundness of the basic ideas and justify further development of these methods for clinical use.

Adult↗

A method for determining total protein of isolated cellular elements and corresponding tritium radioactivity.

A method is described for the microanalysis of protein, obtained from isolated tissue elements, in the range of 500 micromicrog-500 mmicrog. The method entails solubilization of cellular protein with phosphoric acid and heat after extraction of acid-soluble compounds, lipids, and RNA. A procedure for the extraction and recovery of cellular RNA by the use of 40% trichloroacetic acid is presented. The solubilized protein, in the form of a microdroplet, is photomicrographed with monochromatic light at 230 mmicro. Total density in the microdroplet is determined from calibrated photographic plates by microdensitometry, and is converted to protein mass by using an experimentally determined average specific absorbance value. A solubilized protein labeled with tritium can be recovered after photomicrography, combusted, and reduced to generate tritiated gas for high-efficiency tritium radiometry. Total protein was analyzed in (a) nerve cells of three different sizes from Deiters' nucleus of the rabbit; and the whole rod cell and rod cell nucleus of the rabbit retina.

Animals↗

Spectral variation of the infrared absorption coefficient in pulsed photothermal profiling of biological samples.

Pulsed photothermal radiometry can be used for non-invasive depth profiling of optically scattering samples, including biological tissues such as human skin. Computational reconstruction of the laser-induced temperature profile from recorded radiometric signals is sensitive to the value of the tissue absorption coefficient in the infrared detection band (muIR). While assumed constant in reported reconstruction algorithms, muIR of human skin varies by two orders of magnitude in the commonly used 3-5 microm detection band. We analyse the problem of selecting the effective absorption coefficient value to be used with such algorithms. In a numerical simulation of photothermal profiling we demonstrate that results can be markedly impaired, unless the reconstruction algorithm is augmented by accounting for spectral variation muIR(lambda). Alternatively, narrowing the detection band to 4.5-5 microm reduces the spectral variation muIR(lambda) to a level that permits the use of the simpler, unaugmented algorithm. Implementation of the latter approach for depth profiling of port wine stain birthmarks in vivo is presented and discussed.

Algorithms↗

A comparative study of human skin thermal response to sapphire contact and cryogen spray cooling.

Surface cooling, in conjunction with various thermally mediated therapeutic procedures, can provide a means to protect superficial tissues from injury while achieving destruction of deeper targeted structures. We have investigated the thermal response of in-vivo human skin to: 1) contact cooling with a sapphire window (6-12 degrees C); and 2) spray cooling with a freon substitute cryogen [tetrafluoroethane; boiling point approximately -26 degrees C at 1 atmospheric pressure (atm)]. Measurements utilizing infrared radiometry show surface temperature reductions from 30 degrees C to 14-19 degrees C are obtained within approximately 1 s in response to sapphire contact cooling. Surface temperature reductions to values between 5 degrees C and -9 degrees C are obtained in response to 20-100-ms cryogen spurts. Computational results, based on fitting the measured radiometric surface temperature to estimate heat transfer parameters, show: 1) temperature reductions remain localized to approximately 200 microns of superficial tissue; and 2) values of heat flux and total energy removed per unit skin surface area at least doubled when using cryogen spray cooling.

Aerosol Propellants↗

Cryogen spray cooling during Nd:YAG laser treatment of hemangiomas. A preliminary animal model study.

BACKGROUND: Successful laser treatment of hemangiomas requires selective photothermal destruction of dilated cutaneous vessels without damaging the overlying epidermis. Delivering a short cryogen spurt, on the order of milliseconds, has been shown to result in localized cooling of the superficial skin structures during laser irradiation. OBJECTIVE: The purpose of this study was to examine the effectiveness of cryogen spray cooling (CSC) in protecting superficial tissue structures during continuous Nd:YAG laser irradiation of an in vivo model hemangioma. METHODS: The highly vascularized chicken comb was selected as the animal model for hemangiomas. The Nd:YAG laser irradiation ranged from 2.6 to 35.1 J/mm2. A feedback system utilizing infrared radiometry monitored the comb surface temperature and controlled delivery time of the cryogen spurt. When comb surface temperature during laser irradiation reached 36-42 degrees C, a 30-100 msec cryogen spurt was delivered. Animals were euthanized 1 hour to 21 days following each experiment. Gross and histologic analyses were performed. RESULTS: Nd:YAG laser irradiation resulted in deep (up to 6.1 mm) tissue photocoagulation, while CSC preserved the overlying epidermis and papillary dermis. CONCLUSION: The results demonstrate that CSC is effective in protecting the epidermis and papillary dermis, while achieving deep tissue photocoagulation during Nd:YAG laser irradiation. Further pilot studies in humans appear warranted.

Aerosols↗

Ground-level solar spectral irradiance in Glasgow: an inter-comparison of two sites.

BACKGROUND: Solar spectral radiometry presents significant challenges to produce accurate and reproducible data. To investigate the reliability of the measurements, several inter-comparisons have been set up. Although these are useful, their main drawback is that equipment must be dismantled and transported to a common site and re-calibrated. METHODS: In this study, an inter-comparison has been performed of two spectroradiometers that are located 3 miles apart some 30 m above sea level. These two systems have operated using different calibration techniques. Data were compared on clear days, to minimise actual differences in ultraviolet irradiation. RESULTS: There were substantial differences at some individual wavelength points, but overall the mean difference of results at 5 nm intervals on an individual scan from the two systems agreed to within 11%. If the data were used to compute the erythemal irradiance, the differences were reduced to 4%. CONCLUSION: This study demonstrates both the limitations and the level of reliability that might be expected from these systems operating under careful scientific supervision.

Geography↗

Use of potassium iodide as a chemical actinometer.

Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is eliminated and stoichiometric formation of triiodide occurs. The absorbance increase due to triiodide was followed and the quantum yield determined using either a radiometer or ferrioxalate actinometry to estimate the amount of energy absorbed by the KI solution. The quantum yield (phi) at 25 degrees C was determined to be 0.26 using radiometry, and 0.224-0.233 using actinometry, depending on the radiation conditions. The following expression was used to measure the incident fluence rate for 254 radiation at a given temperature T for irradiation over a time interval delta t (s) [formula: see text] At temperatures other than 25 degrees C, the temperature dependence of the quantum yield (0.004/degrees C) is taken into account by the term shown in the denominator. Because KI remains relatively blind to longer wavelengths such as those found in normal room light, measurements can be made in the presence of room light without having to work in a darkened room.

Dose-Response Relationship, Radiation↗

Monitoring of experimental arthritis in rabbits.

The effect of ambient temperature and air flow on the radiometric measurement of experimental arthritis in rabbit knee joints has been studied. Temperature changes due to inflammation in such joints could be detected up to 70 days after induction of the arthritis by the use of radiometry. The method has been used to quantitate the anti-inflammatory activity of intra-articularly injected cortisol acetate and orally administered aspirin. It is suggested that this method of noninvasive monitoring has a number of advantages over other procedures.

Animals↗

Effects of media, working practice, and automation on the rapid detection of bacteraemia.

Advances in the treatment of disease have resulted in an increasing number of blood cultures being processed by microbiology departments. The effect of media, revision of working hours, and automation on the time taken to detect positive cultures are reported. A 9.5% detection rate at 24 h with broth systems examined manually increased to 76.0% with a semi-automated radiometric technique (BACTEC). The use of radiometry in a restructured working day--that is, 9.00 am to 7.00 pm--resulted in optimum conditions for reducing the reporting time. The efficacy of modern equipment, designed to detect micro-organisms rapidly, can be considerably enhanced by reorganisation of traditional working hours to suit microbial growth rather than the current availability of staff.

Bacteria↗

Comparison of techniques for antimicrobial susceptibility testing of mycobacteria.

AIMS: To evaluate adenosine triphosphate (ATP) bioluminescence as a rapid technique for antimicrobial susceptibility testing of Mycobacterium spp by comparing it with conventional and radiometric methods, and to assess its potential for use in clinical microbiology laboratories. METHODS: 115 clinical isolates from a wide range of mycobacterial species and four control organisms of known susceptibility were tested against six antimicrobial agents. Minimum inhibitory concentrations (MICs) were determined after 4-6 weeks' incubation on Middlebrook 7H10 agar. Susceptibility was also determined radiometrically using a Bactec 460, and by bioluminescent assay of ATP using a 1250 luminometer (LKB-Wallac). RESULTS: Susceptibility results after 7 days showed excellent correlation with conventionally determined MICs. 714 susceptibility tests were performed by both techniques, with seven major discrepancies between the two systems. For pyrazinamide, agreement was 100%, but five strains of M tuberculosis, including one control, and 11 mycobacteria other than M tuberculosis (MOTT) failed to grow on Middlebrook agar at pH 5.5. 606 tests were performed by radiometry, with four major discrepancies between this technique and ATP bioluminescence. No particular species of Mycobacterium gave aberrant results. Contamination was a problem; 12 of the 119 strains tested were contaminated at day 1 and had to be repeated before results were obtained. Contamination of individual tests increased significantly after 7 days of incubation. CONCLUSIONS: ATP bioluminescence can be used to monitor mycobacterial growth in fluid culture media; the technique has considerable potential for rapid susceptibility testing. Advantages include lower initial cost of analytical equipment, lower reagent cost per test, and the use of non-radioactive substrates.

Adenosine Triphosphate↗

Experimental thermoradiotherapy of human tumour xenografts in nude mice: design of the hyperthermia system.

An experimental thermoradiotherapy study was started in 1986. For this study a hyperthermia system was developed for the heating of human tumours xenotransplanted into nude mice. Our treatment device was a four-channel computer-controlled hyperthermia system. Temperature was monitored by microwave radiometry at 3 GHz. Specifications of the radiometer were first evaluated under reference conditions, then thermal dosimetry was studied using non-invasive measurement of the brightness temperature TOR. Hyperthermia treatments were simulated in phantom material with radiometric monitor values TR of 40 and 41. In conjunction with the parameters of brightness temperature TOR, surface temperature TO and water bolus temperature TW thermal modelling was performed. Finally, we studied the influence of a perfused phantom to the microwave thermometry.

Animals↗

Comparison of radiometric and histometric features of bone samples from ribs.

OBJECTIVE: To correlate quantitative data gathered from conventional radiograms as well as from quantitative computed tomography (QCT) images of bone samples with histometric coefficients of the same specimens. STUDY DESIGN: Bone samples were obtained from standardized sites of ribs during autopsy of 40 males aged 15-84 years. In the bone samples the following assessments were performed: densitometry of bone using QCT, estimation of radiostructural quantitative coefficients (coefficient of trabecular arrangement anisotropy, trabecular thickness coefficient); histometry (area of bone matrix, trabecular thickness coefficient); relative weights of dry, decalcified bone and bone in ash form; and concentration of calcium in ash. Radiometry and histometry were performed using image analysis methods with IBAS for Windows (Kontron, Eching b. München, Germany) equipment. Radiostructure of bone was evaluated with the use of our own algorithm; concentration of calcium was measured spectrophotometrically. Analysis of variance, cross correlation, regression analysis and others were the statistical tools applied. RESULTS: From the variables analyzed, the following indicates a significant correlation with the histometric area of bone matrix: radiologic trabecular anisotropy, QCT densitometry (especially of cancellous bone) and the ratio of weight of dry sample to fresh sample. The coefficient of radiologic trabecular thickness appeared not to be a valuable feature for estimation of bone histology. CONCLUSION: Quantitative estimation of radiostructure of bone carried out on conventional radiograms may be helpful in the detection of histologic properties of bone, an important factor in assessment of the fragility of bone.

Adolescent↗

The use of radiometric tests in the speciation of mycobacteria--a review.

Besides a more rapid detection and susceptibility testing, radiometry offers possibilities for the identification of mycobacteria. This paper gives a chronological summary of radiometric techniques used in the BACTEC system (Becton Dickinson) for the identification of mycobacterial complexes/species, employing chemical compounds as selective inhibitory agents or for testing biochemical activities. Thirteen tests are presented and discussed.

Mycobacterium↗

Thermography and rheumatic diseases.

The common factor in most of the rheumatic diseases is an arthritis. Radiometry and thermography have been shown to indicate and measure heat resulting from localised inflammation. In rheumatoid arthritis, juvenile arthritis, osteoarthrosis, gout and ankylosing spondylitis abnormal heat distribution has been recorded over affected joints. Experimental evidence has shown that temperature change reflects the inflammatory state of the joint, and that this may be used to measure the effect of therapy by oral, systemic and local drug therapy, and also surgery, i.e. synovectomy.

Adrenocorticotropic Hormone↗

[Heterogeneity of the population of Escherichia coli thr- by assimilation of 3H-threonine].

The incorporation and spatial localisation of [3H]threonine in the cells of Escherichia coli RP 477 (leu-his-thr-B1-) were studied using the techniques of radiometry and adsorption electron-microscopic autoradiography with additional time-lapse microfilming to follow up the growth of the population. The strain was shown to be heterogeneous in its metabolic, reproductive and morphological (cell diameter) characteristics; a generative cluster was revealed. Indirect data indicated that the so-called "resting" cells were functionally active.

Escherichia coli↗

[Radiothermometry in intracavitary hyperthermia].

The potentialities of microwave radiometry application have been considered as an adjuvant to invasive thermometry to control temperature during local MWF-hyperthermia. An increasing role of radiometric monitoring in intracavitary hyperthermia has been shown in experiments on heating rectal tumors. Technical aspects of radiometric temperature measurements combined with MWF-hyperthermia are discussed.

Body Temperature↗

[Methods available for measuring temperature (author's transl)].

Numerous techniques are in use in medicine for temperature determination either from thermal conduction phenomena, or from hot body radiations. These are essentially thermometry, radiometry and thermography. During attempts to treat cancer by hyperthermia, temperature knowledge, at the level of the target volume and the adjoining parts, is essential. These deep temperature measurements are yet a problem and certainly need new sensor technologies.

Body Temperature↗

Rapid presumptive identification of the Mycobacterium tuberculosis-bovis complex by radiometric determination of heat stable urease.

Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M. bovis (5), M. kansasii (15), M. marinum (4), M. simiae (3), M. scrofulaceum (16), M. gordonae (6), M. szulgai (6), M. flavescens (1), M. gastri (1), M. intracellulare (6), M. fortuitum-chelonei complex (12), and M. smegmatis (1)--were tested for neat urease activity by Bactec radiometry. Mycobacterial isolates (50-100 mg wet weight) were incubated at 35 degrees C for 30 minutes with microCi14C-urea. Urease-positive mycobacteria gave Bactec growth index (GI) values greater than 100 units, whereas urease-negative species gave values less than 10 GI units. Eighty-three isolates possessing neat urease activity were heated at 80 degrees C for 30 minutes followed by incubation at 35 degrees C for 30 minutes with 1 microCi14C-urea. Mycobacterium tuberculosis-bovis complex demonstrated heat-stable urease activity (GI more than 130 units) and could be distinguished from mycobacteria other than tuberculosis (MOTT), which gave GI values equal to or less than 40 units.

Drug Stability↗