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Diplostomum (Austrodiplostomum) compactum (Lutz, 1928) (Platyhelminthes, Digenea) metacercariae in fish from the floodplain of the Upper Paraná River, Brazil.

Diplostomum (Austrodiplostomum) compactum (Lutz, 1928) metacercariae (Platyhelminthes, Digenea) were found in six fish species, belonging to two orders (Characiformes and Perciformes) and three families (Erythrinidae, Sciaenidae and Cichlidae). A total of 477 individuals were collected, from August 1999 to May 2001, in the upper Paraná River floodplain, Brazil. The metacercariae were infecting the eyes of six host species and the brain of five of them. Plagioscion squamosissimus, Satanoperca pappaterra and Cichla monoculus presented the highest values of prevalence, mean intensity and mean abundance. Diplostomum (A.) compactum was over-dispersed in the population of Hoplias aff. malabaricus, S. pappaterra, P. squamosissimus, Crenicichla britskii and C. monoculus. No significant difference was observed in the metacercariae distribution in the right and left eyes. The abundance of D. (A.) compactum metacercariae was positively correlated with the host's relative condition factor in H. aff. malabaricus and S. pappaterra. No significant difference of the abundance of parasitism between the sex of the hosts was evidenced. In relation to the prevalence, significant difference was observed only in C. britskii. Positive correlations were verified between the prevalence of infection and the standard length of the specimens in C. britskii and the intensity of infection and the standard length of the hosts in P. squamosissimus and C. monoculus. In relation to parasite abundance and the standard length class of the host, significant differences were observed in P. squamosissimus, C. britskii and C. monoculus.

Animals↗

Light microscopic description of Eimeria sparis sp. nov. and Goussia sparis sp. nov. (Protozoa:Apicomplexa) from Sparus aurata L. (Pisces: Teleostei).

Two new species of Coccidia, Eimeria sparis sp. nov. and Goussia sparis sp. nov., were found in the intestine of Sparus aurata from different culture systems of Spain. These are the first coccidian species described from this host. E. sparis is distinguished from other Eimeria spp. from Perciformes in the size and shape of the sporocysts, which measure 6-9.7 (mean = 7.78, SD = 1.33) x4-6.5 (5.3 +/- 0.92) microns, and in the Stieda-like body. Mature oocysts, spherical or subspherical, measure 9.4-14.3 (11.88 +/- 1.85) microns. G. sparis differs from other Goussia spp. from fish of the Mediterranean area in the size and shape of the oocysts and sporocysts as well as in the location. Mature oocysts measure 16-21 (17.4 +/- 1.5) x 13-18 (14.4 +/- 1.7) microns and sporocysts, 8.6-10.3 (9.5 +/- 0.5) x 5.7-7.4 (mean 6.5 +/- 0.5) microns. The different stages of merogony, gamogony, and sporogony of both species were examined at the light-microscope level in fresh material and histology specimens. Sporulation was endogenous in E. sparis and exogenous in G. sparis.

Animals↗

The complete mitochondrial genome sequence and characterization of single-nucleotide polymorphisms in the control region of the Asian seabass (Lates calcarifer).

We determined the complete mtDNA nucleotide sequence of Lates calcarifer using the shotgun sequencing method. The mitochondrial DNA (mtDNA) was 16,535 base pairs (bp) in length, and contained 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and one major noncoding control region (CR). The CR was unusually short at only 768 bp. A striking feature of the mitochondrial genome was the high G+C content (46.1%), which is among the highest in fish. The gene order was identical to that of a typical vertebrate. Phylogenetic analyses using concatenated amino acid sequences of 12 protein-coding genes of 30 fish species representing 14 suborders clearly showed Lates calcarifer was located in the cluster of fish species from the order Perciformes, supporting the traditional systematic classification. We characterized single-nucleotide polymorphisms (SNPs) in the CR by sequencing the complete CR of 25 individuals obtained from Australia and Singapore. A total of 68 SNPs were detected. Eighteen SNPs were fixed with alternative nucleotides in Australian and Singapore seabass, and these SNPs could be used for differentiating fish from the two countries.

Animals↗

Assessing genetic diversity of Brazilian reef fishes by chromosomal and DNA markers.

Little is known on genetics of Brazilian coral reef fish and most of this information is limited to chromosome characterization of major representative species. The diploid chromosome number in marine fish varies from 2n= 22-26 to 2n = 240-260. Despite of this apparent diversity, most studied marine species have a diploid complement with 48 acrocentric chromosomes. This latter trend is mostly observed among Perciformes, an important major taxon of coral reef fishes. Studies in the families Pomacentridae, Pomacanthidae and Chaetodontidae, for example, have shown a common karyotype pattern entirely formed by 48 uniarmed chromosomes. However, rare numerical and structural chromosome polymorphisms and cryptic chromosome rearrangements involving heterochromatin segments and/or nucleolar organizing sites have been reported among such fishes. Although new chromosome forms can contribute to the establishment of genetically isolated populations, their role in reef fish speciation at marine realm still is an open question. More recently, genomic DNA analyses using RAPD and microsatellites, and sequencing and RFLP of mitochondrial DNA have increasingly been used in Atlantic reef fish species. Genetic homogeneity over wide geographical ranges has been reported for different fish groups, in contrast to several cases of population substructuring related to environmental constraints or evolutionary history. Amazonas outflow and upwelling on the Southeastern coast of Brazil are believed to be strong barriers to dispersal of some reef species. Moreover, it is suggested that the pattern of speciation and population structure at South Atlantic is quite distinctive from Pacific Ocean, even when comparing closely related taxa. Further genetic studies are strongly encouraged in Brazilian reef fishes in order to provide a reliable scenario of the genetic structure in this important and diverse fish group.

Animals↗

Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish.

The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.

Animals↗

Purification of maturational gonadotropin from Atlantic croaker (Micropogonias undulatus) and development of a homologous radioimmunoassay.

The isolation of a maturational gonadotropin from pituitaries of Atlantic croaker (Micropogonias undulatus), a marine teleost belonging to the order perciformes, is described. A radioimmunoassay has been developed for the measurement of this hormone in blood samples and validation of the assay is presented. The hormone measured is an acidic isoform of GTH and can be detected in the plasma at concentrations from 25 to 0.05 ng/ml. At least one other gonadotropic fraction was detected during the purification. The acidic form of GTH is released from croaker pituitaries after stimulation with luteinizing hormone-releasing hormone analog (LHRHa), both in vivo and in vitro. The GTH is steroidogenic, is active in an in vitro oocyte germinal-vesicle breakdown assay, and is elevated in the blood of fish undergoing LHRHa-induced ovulation.

Amino Acids↗

Adaptative features of enzymes from family sciaenidae--II. Studies on phosphoglucose isomerase (PGI) of fishes from the south coast of Uruguay.

1. The electrokinetic and thermostability properties of phosphoglucose isomerase (PGI) in three species of temperate fishes (Perciformes, Acanthopterygii)--Cynoscion striatus, Macrodon ancylodon and Micropogonias furnieri--have been analyzed in order to study the adaptative 2. Unlike most diploid fishes the PGI of these species seemed to be encoded by three PGI loci. 3. The subunits encoded by these loci occurred at different levels in the different tissues and organs analyzed. 4. Genetic variants at two loci (Pgi-A and Pgi-B2) were detected in Cynoscion striatus, and at one (Pgi-A) in Micropogonias furnieri. 5. The product of these loci could be separated in three PGI regions based on their electrophoretic distribution and thermostability properties. 6. The more anodal region (including isozymes and allozymes) was more thermolabile than the less anodal one, which is predominant in a single tissue skeletal muscle. 7. Relative activities of PGI isozymes were compared by Klebe's method to determine pattern of divergence of duplicated gene expression in the three species studied.

Adaptation, Physiological↗

Adaptative features of enzymes from family Sciaenidae--III. Studies on lactate dehydrogenase (LDH) of fishes from the south coast of Uruguay.

1. Electrophoretic analyses of lactate dehydrogenase isozyme patterns of three species of temperate fish belonging to family Sciaenidae (order Perciformes) indicates that at least three LDH loci--Ldh-A, Ldh-B and Ldh-C are active. 2. The subunits encoded by these loci occurred at different levels in the different tissues and organs analyzed. 3. The products of these loci show different response to changes in temperature. The results were obtained by electrophoretic analyses. 4. Thermostability of skeletal muscle, heart and eye lactate dehydrogenase incubated at 60 degrees C for different periods of time were examined. 5. Relative activities of LDH isozymes were compared by Klebe's method to determine pattern of divergence of duplicated gene expression in the three species studied.

Acclimatization↗

An analysis of esterase activities from surgeonfish tissues yields evidence of an atypical pseudocholinesterase.

1. Esterases from tissues of the surgeonfish (Teleostei, Perciformes, Acanthuridae) are characterized electrophoretically and include several carboxylesterases, an acetylesterase, and an atypical pseudocholinesterase (pseudo-ChE). 2. The pseudo-ChE occurs in several isozymic forms including sialated and asialated slightly-anodal forms found principally in liver, and a larger, asialated asymmetric form that barely penetrates the 10% PAGE gel matrix found together with true AChE in epaxial muscle, brain, and eye. 3. Characterization of these three pseudo-ChE activities suggest that they are decidedly atypical in the intermediacy of their substrate and inhibitor specificities relative to classically-defined AChE and pseudo-ChE activities.

Animals↗

Antifreeze glycopeptides of the high-Antarctic silverfish Pleuragramma antarcticum (Notothenioidei).

Antifreeze glycopeptides (AFGP) have been isolated from the fully pelagic high-Antarctic silverfish Pleuragramma antarcticum of the suborder Notothenioidei (Perciformes). The fishes were caught during the PRV Polarstern expedition EPOS III (Jan-Mar, 1989) in the eastern and southeastern Weddell Sea. Glycoconjugate and amino acid analysis of antifreeze glycopeptides (AFGP) indicate that the glycopeptide structure is identical to the polymers of H2N[Ala-Ala(beta-galactosyl(1-->3)-alpha-N- acetylgalactosamine)Thr]nAla-Ala-COOH of previously studied Antarctic notothenioids. The content of AFGPs in P. antarcticum is lower than in other notothenioid fish from the same region. Antifreeze activity shows a maximal hysteresis of 1.19 degrees C at a concentration of 20 mg/ml AFGP. A linear increase in activity of the antifreeze glycopeptides could be demonstrated concomittant with a decreasing ice content. The freezing point of blood serum is -1.9 degrees C.

Acetylgalactosamine↗

Olfactory sensory input increases gill ventilation in male round gobies (Neogobius melanostomus) during exposure to steroids.

In teleostean fish, ventilation increases have been observed in response to low dissolved oxygen levels, visual stimuli, and gustatory cues. However, olfactory sensory input may also stimulate gill ventilation rate. We investigated whether olfactory sensory input mediates gill ventilation responses, as suggested by the observation that steroidal compounds detected by the olfactory system elicited increases in opercular activity in the perciform teleost, the round goby (Neogobius melanostomus). Close parallels between gill ventilation and olfactory responses, led us to conduct an empirical study that used two different olfactory sensory deprivation techniques to seek a causal relationship between olfactory epithelial activity and hyperventilation. Chemical lesion of olfactory sensory neurons or mechanical occlusion of the nasal cavities inhibited gill ventilation responses of reproductive male round gobies to estrone (1,3,5(10)-estratrien-3-ol-17-one) and to ovarian extracts. This direct evidence demonstrates the role of olfactory sensory input for the gill ventilation response to putative reproductive pheromones and may represent an important regulatory mechanism for odorant sampling during pheromone communication.

Animals↗

Molecular cloning of cDNAs and structural model analysis of two gonadotropin beta-subunits of snakehead fish (Channa maculata).

The cDNAs encoding beta-subunits of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have been cloned from the pituitary of snakehead fish, Channa maculata, and the three-dimensional structural models of the encoded FSH and LH were investigated. The cloned cDNAs, including 5'-untranslated region (UTR), open-reading frame, and 3'-UTR followed by a poly(A) tail, were obtained by reverse transcription-polymerase chain reaction and rapid amplification of cDNA end methods. The open-reading frames of FSH-beta cDNA encodes a 120-amino acid protein with a signal peptide of 18 amino acids and a mature protein of 102 amino acids; while LH-beta cDNA encodes a 140-amino acid protein with a signal peptide of 33 amino acids and a mature protein of 115 amino acids. The amino acid sequence identities of snakehead fish FSH-beta and LH-beta in comparison with other fish are 27.8-81.9% and 45.2-88.8%, respectively; while in comparison with tetrapods are 26.2-28.9% and 37.5-51.2%, respectively. Both FSH-beta and LH-beta of snakehead fish resemble most to those of Perciformes, implying their closer phylogenetic relationship. All 12 cysteine residues are conserved in snakehead fish LH-beta; while 11 cysteine residues are conserved in its FSH-beta. The third cysteine is absent in snakehead fish FSH-beta; instead, a positionally shifted cysteine residue is present at the N-terminus, as found in some phylogenetic related fish. The structure models of snakehead fish FSH and LH, constructed by using the crystal structures of human FSH and human chorionic gonadotropin as respective template, showed that the positionally shifted N-terminal cysteine residue of snakehead fish FSH-beta likely can substitute the third cysteine to form a disulfide bond with the 12th cysteine.

Amino Acid Sequence↗

The fish endocrine pancreas: review, new data, and future research directions in ontogeny and phylogeny.

The literature on the ontogeny and phylogeny of the endocrine pancreas of ray-finned fishes is summarized since the latest review in fish [Youson, J.H., Al-Mahrouki, A.A., 1999. Review. Ontogenetic and phylogenetic development of the endocrine pancreas (islet organ) in fishes. Gen. Comp. Endocrinol. 116, 303-335]. A basic description and a demonstration of the diversity of the fish islet organ is provided through new immunohistochemical data on islet tissue from a basal teleost, an osteoglossomorph, and a more derived teleost, a perciforme. Unlike the previous review, the present report provides a review and discussion of the utility of sequence data of insulin, somatostatin, and NPY- and glucagon-family peptides in phylogenetic analyses of jawed and jawless fishes. The present study also provides the first comparative analysis of sequences of preprohormones of endocrine peptides from closely related basal teleost species. Some nucleotide and deduced amino acid sequence data for preprosomatostatins (PPSS-I and/or -II) are compared for four species of bonytongues, Osteoglossomorpha, and with PPSSs of the white sucker, Catostomus commersoni, representing Cypriniformes, a more generalized teleost order. Phylogenetic analysis of deduced amino acid sequences of the PPSSs of these species and others from databases indicates good support for the monophyly of Osteoglossomorpha and some support for the present taxonomic grouping of the osteoglossomorphs examined, and also the white sucker. However, PPSS may have limited phylogenetic utility due to the relative short sequence, particularly in resolving relationships among lineages that diverged over a short period of time. Since in the few fish species examined we have just touched the surface in describing the diversity of structure of the islet organ, and likely the nature of the products of its cells, this report promotes the continued study of this organ.

Amino Acid Sequence↗

A phylogeny of the fish family Sparidae (porgies) inferred from mitochondrial sequence data.

The porgies (Sparidae) comprise a diverse group of neritic fishes with a broad geographic distribution. We used mitochondrial DNA sequences from partial 16S ribosomal RNA and cytochrome b genes to reconstruct the phylogenetic history of these fishes. Sequences from 38 sparid species, 10 species in outgroups closely related to sparids, seven basal percoid species, and a non-perciform outgroup species were analyzed with parsimony and maximum likelihood. The Sparidae were monophyletic with the inclusion of Spicara, which is currently placed in the Centracanthidae. The genera Spicara, Pagrus, and Pagellus, were not monophyletic indicating a need for revision. Two main sparid lineages were recovered in all analyses, but the previously proposed six sparid subfamilies (Boopsinae, Denticinae, Diplodinae, Pagellinae, Pagrinae, and Sparinae) were not monophyletic. This suggests that dentition and feeding modes, upon which these subfamilies are based, were independently derived multiple times within sparid fishes. There was no evidence from the 16S or combined analyses for a monophyletic Sparoidea.

Animals↗

Does lymphocystis occur in pacora, Plagioscion surinamensis (Sciaenidae), from Colombia?

A lymphocystis-like condition produced hemorrhagic lesions over the bodies of pacora Plagioscion surinamensis (Bleeker) (Perciformes: Sciaenidae) held in freshwater aquaculture in northern Colombia and increased their sensitivity to handling stress. This disease eliminated this fish from a project evaluating its aquaculture potential. Lymphocystis disease is assumed to be less damaging to the host. This condition requires more study and histological evaluation.

Animals↗

Interarm interaction of DNA cruciform forming at a short inverted repeat sequence.

A novel interarm interaction of DNA cruciform forming at inverted repeat sequence was characterized using an S1 nuclease digestion, permanganate oxidation, and microscopic imaging. An inverted repeat consisting of 17 bp complementary sequences was isolated from the bluegill sunfish Lepomis macrochirus (Perciformes) and subcloned into the pUC19 plasmid, after which the supercoiled recombinant plasmid was subjected to enzymatic and chemical modification. In high salt conditions (200 mM NaCl, or 100-200 mM KCl), S1 nuclease cut supercoiled DNA at the center of palindromic symmetry, suggesting the formation of DNA cruciform. On the other hand, S1 nuclease in the presence of 150 mM NaCl or less cleaved mainly the 3'-half of the repeat, thereby forming an unusual structure in which the 3'-half of the inverted repeat, but not the 5'-half, was retained as an unpaired strand. Permanganate oxidation profiles also supported the presence of single-stranded part in the 3'-half of the inverted repeat in addition to the center of the symmetry. Both electron microscopy and atomic force microscopy have detected a thick protrusion on the supercoiled DNA harboring the inverted repeat. We hypothesize that the cruciform hairpins at conditions favoring triplex formation adopt a parallel side-by-side orientation of the arms allowing the interaction between them supposedly stabilized by hydrogen bonding of base triads.

Animals↗

Chemical interference with genomic and nongenomic actions of steroids in fishes: role of receptor binding.

The characteristics of steroid nuclear and membrane receptors and their interactions with xenobiotic chemicals in two marine perciform species, Atlantic croaker (Micropogonias undulatus) and spotted seatrout (Cynoscion nebulosus) are briefly reviewed. Several organochlorines that bind to the nuclear progesterone receptor in mammals show negligible binding to the nuclear progestogen receptor in seatrout ovaries. Two distinct nuclear androgen receptors with different tissue distributions have been identified in croaker, but only one of them binds xenobiotic anti-androgens previously identified in mammals. Multiple forms of the nuclear estrogen receptor (ER) have been identified in fishes. The ER in croaker testis has a higher affinity than the croaker liver ER for estrogens and xenoestrogens and may be more susceptible to chemical interference. In addition, differences in the feedback effects of estrogens and xenoestrogens on gonadotropin secretion in croaker are observed, depending on the stage of the reproductive cycle. Finally, the first clear evidence in any vertebrate for xenobiotic chemical interference with the nongenomic actions of steroids by binding to steroid membrane receptors was obtained with the seatrout ovarian progestogen membrane receptor and since has been confirmed with progestogen and estrogen membrane receptors in croaker sperm and testes. These various factors that influence chemical/steroid receptor interactions are likely to significantly modify steroid hormone actions at target tissues and consequently the toxicological effects of chemical exposure.

Animals↗

Evolutionary aspects of gobioid fishes based upon a phylogenetic analysis of mitochondrial cytochrome B genes.

The Gobioidei is a large suborder in the order Perciformes and consists of more than 2000 species belonging to about 270 genera. The vast number of species and their morphological specialization adapted to diverse habits and habitats makes the classification of the gobioid fishes very difficult.A comprehensive estimation of the evolutionary scenario of all gobioid fishes using only morphological information is difficult for two major reasons: first, in addition to wide ecological diversification, there is a trend towards specialization and degeneration of morphological characters among these species; second, an appropriate outgroup of gobioid fishes has not been recognized. Based upon nucleotide sequence comparisons of gobioid mitochondrial cytochrome b genes, we established the phylogenetic relationships of their differentiation into many groups of morphological and ecological diversity. The phylogenetic trees obtained show that most species examined have diverged from each other almost simultaneously or during an extremely short period of time.

Animals↗