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At least 271 records · Page 15Linked to original sources
PubMed1964

AGAR-GEL PRECIPITIN TECHNIQUE IN ANTHRAX ANTIBODY DETERMINATIONS.

A modification of the agar-gel precipitation inhibition technique of Thorne and Belton for detecting anthrax antibodies reduces inconsistency of visually determined end points on the same sera observed by different technicians. Determination of the minimal reacting concentrations of the anthrax antigen and antibody reagents, modifications of the visualization apparatus, methods for combining reagents, and length of incubation periods contribute to the ease of the end-point determinations and the uniformity of results. When compared with the previous technique, the modified procedure is less time-consuming while retaining satisfactory reproducibility, simplicity, specificity, and sensitivity.

Agar↗
PubMed1965

AGAR-GEL PRECIPITIN-INHIBITION TECHNIQUE FOR C-REACTIVE PROTEIN DETERMINATIONS. 3. QUANTITATION OF C-REACTIVE PROTEIN IN SERUM SPECIMENS.

A quantitative C-reactive protein serological procedure has been developed. By use of this method, which is performed in agar-gel plates, from 2 to 654 mug of C-reactive protein per ml of titrated human serum can be detected. The method is based on the inhibition of a specific C-reactive protein antigen-antibody precipitate formed in agar-gel by the minimal reactive dilutions of each reagent in 48 hr. It is simple, sensitive, and readily reproducible.

Agar↗