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PubMed1976

A comparison of endometrial histology with simultaneous plasma progesterone determinations in infertile women.

A retrospective analysis of 238 endometrial biopsies and simultaneous plasma progesterone determinations performed during the course of infertility evaluations is presented. The data suggest a high (93%) correlation between the two procedures for the detection of ovulation. Endometrial abnormalities were detected in 5% of the biopsies. Shortening of the cycle was noted in 36% of the patients, while only 3% demonstrated a longer cycle. Simultaneous measurement of plasma progesterone levels in association with the endometrial biopsy is believed to better document corpus luteum defects. One should be aware of the frequent occurrence of a shortened cycle on the basis of postbiopsy bleeding. Basal body temperature graphs should be utilized prior to biopsy in patients with a history of irregular menstrual cycles.

Endometrium↗
PubMed1994

Insemination results with slow-cooled stallion semen stored for approximately 40 hours.

Semen from 3 stallions was extended using 2 methods (Kenney extender and a modified Kenney extender), slowly cooled, and stored for 41 +/- 6 (s.d.) h before insemination. An insemination dose (40 ml) contained 1.5-2 billion spermatozoa. In the experiment, 26 mares were inseminated in 30 cycles. The pregnancy rate per cycle obtained with sperm stored in the Kenney extender was 87% (n = 15). When the semen was extended with the modified extender, centrifuged and stored, the pregnancy rate was 60% (n = 15). Inseminations were done every other day until ovulation was detected. If a mare ovulated more than 24 h after the last insemination, she was inseminated also after ovulation. The single-cycle pregnancy rate was 58% when the mares were inseminated only before ovulation (n = 19) but the rate was 100% when the inseminations were done both before and after ovulation (n = 9) or only after ovulation (n = 2). The difference in pregnancy rates was significant (p < 0.05), indicating that postovulatory inseminations probably serve to ensure the pregnancies. The extending and handling methods used in this study resulted in a combined pregnancy rate of 73%, and appear thus to be useful for storing stallion semen for approximately 2 days.

Animals↗
PubMedDate not supplied

Methods used to self-predict ovulation. A comparative study.

Time of ovulation as detected by a self-test of luteinizing hormone (LH) in the urine was compared with time of ovulation as detected by self-observation of cervical mucus. Twenty regularly cycling women monitored their cervical mucus and urine LH for two complete menstrual cycles. Of the cycles that had an LH surge, 100% were on the peak day of cervical mucus or within three days before the peak day. Self-observation of cervical mucus, therefore, can be an accurate method of determining optimal fertility.

Adult↗
PubMed2004

Synchronization of ovulation in yaks (Poephagus grunniens L.) using PGF(2alpha) and GnRH.

The objective of this study was to test the efficacy of estrus synchronization in yaks using the Ovsynch protocol. To eight non-lactating cycling yaks were administered GnRH analogue followed by PGF(2alpha) analogue treatment 7 days later and further injected with a second injection of same GnRH analogue 2 days after the PGF(2alpha) analogue administration. Ovulation was detected by rectal palpation at 2 h intervals from the initial signs of estrus till ovulation. For LH and progesterone the blood samples were collected at 15 min intervals starting from 1 h prior to the second injection of GnRH analogue until 6 h later and further at 2 h intervals till 2 h after the ovulation. Ovulation was detected in seven out of eight yaks after Ovsynch treatment. The mean time interval from the second GnRH injection to ovulation was 24.8+/-1.95 h with a range of 20-34 h and the mean interval from the LH peak and ovulation was 19.96+/-1.91 h with a range of 14-29 h. The high degree of ovulation synchronization could be attributed to the highly synchronized LH peaks in the treated animals. It was concluded that this estrus synchronization protocol could be applied for fixed time AI in yak.

Animals↗
PubMed1978

Changes in salivary peroxidase and polymorphonuclear neutrophil leucocyte enzyme activities during the menstrual cycle.

An elevation in salivary peroxidase activity has been found about the time of ovulation in 14 menstrual cycles in a total of six women. This peak coincided with the ovulatory luteinizing hormone (LH) and oestrogen peak in the four cycles in which endocrine studies were performed. Rises in some polymorphonuclear neutrophil leucocyte enzymes were also seen around ovulation. The possible use of changes in salivary peroxidase as a method for detection of ovulation is discussed.

Adolescent↗
PubMed2003

Day of cycle affects changes in equine intrauterine pressure in response to teasing.

Oxytocin is released in response to teasing during both estrus and diestrus in mares, and at least during estrus, teasing results in an increase in electromyographic activity in the uterus. Exogenous oxytocin causes an increase in intrauterine pressure and prior studies have shown that this response is correlated to the day of the estrous cycle. To determine if teasing causes an increase in intrauterine pressure and if this response varies by day of the cycle, intrauterine pressure was measured while mares were teased with a stallion 2 days before ovulation, on the day ovulation was detected and 2 days after ovulation. A significant increase in intrauterine pressure was observed in response to teasing both 2 days before ovulation and on the day of ovulation, when plasma concentrations of progesterone were low. No significant increase in intrauterine pressure was observed in response to teasing 2 days after ovulation when progesterone concentrations were elevated. Management practices that include teasing or stallion exposure may be beneficial in stimulating uterine clearance mechanisms in mares during the preovulatory period.

Animals↗
PubMed1988

Reproductive exocrine and endocrine profile of female langur monkeys, Presbytis entellus.

In 5 caged langurs menstrual cycle length (for 125 cycles) varied from 18 to 45 days with a mean +/- s.d. of 26.3 +/- 3.6 days. Menstruation lasted for 2 days. A distinct cycle of vaginal smear cells was observed with a maximum cornification on Day 10 coinciding with the serum oestradiol peak. A monophasic pattern of vaginal temperature with a significant nadir on Day 11, 1 day after the oestradiol peak, was observed. Serum sialic acid concentrations directly reflected the oestrogenic activity and showed a significant peak on Day 10, coinciding with the oestradiol peak and was followed by a progesterone rise. Langurs menstruated throughout the year without showing any sign of summer amenorrhoea. We suggest that maximum cornification, vaginal temperature nadir and serum sialic acid peak could be used as markers for prediction and detection of the time of ovulation in langur monkeys.

Animals↗
PubMed2005

Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes (Bubalus bubalis).

Two experiments were conducted to assess the timing and synchrony of ovulation, plasma LH concentrations, and pregnancy rate in Murrah buffaloes (Bubalus bubalis) treated with the Ovsynch (GnRH-PGF(2 alpha)-GnRH) protocol. In Experiment 1, 10 non-lactating cycling buffaloes received 10 microg of a GnRH analogue i.m. (buserelin acetate) without regard to the stage of the estrous cycle (day of treatment, day 0), followed by 25mg of PGF(2 alpha) i.m. (dinoprost thromethamine) 7 days later. A second-treatment of the same GnRH analogue (10 microg, i.m.) was given 48 h after PGF(2 alpha). Ovulation was confirmed by transrectal palpation (at 2-h intervals) from the second-GnRH treatment to detection of ovulation or up to 96 h after the second-GnRH treatment. Plasma LH concentrations were determined in blood samples collected at 15-min intervals for 6h, starting at the second-GnRH treatment, and thereafter at 2-h intervals until 2h after detection of ovulation. Ovulation occurred in 9/10 buffalo (90%) 23.3+/-1.3h (mean+/-S.E.M.; range 20--32 h) after the second-GnRH treatment. Peak LH concentrations 13.5+/-3.5 ng/mL (range 3.9--40.0 ng/mL) occurred 2.1+/-0.1h (range 1.2-3.0 h) after the second-GnRH treatment. In Experiment 2, 15 lactating, cycling buffaloes were subjected to the Ovsynch protocol, with fixed-time AI 12 and 24h after the second-GnRH treatment and 75 lactating buffaloes were inseminated, approximately 12h after detection of spontaneous estrus. Pregnancy rates were 33.3% for TAI and were 30.7% for buffaloes inseminated following spontaneous estrus (P=0.84). In conclusion, the Ovsynch protocol effectively synchronized ovulation in Murrah buffaloes and resulted in conception rates (to two fixed-time inseminations) that were comparable to those achieved with a single AI after detection of spontaneous estrus.

Animals↗
PubMed1990

Detection of the time of ovulation in clomiphene stimulated cycles with an easily applicable urine test.

40 cycles of 35 patients, 38 of which were stimulated with clomiphen, were monitored with the LH-Colortest. This urine test, which was applied by the patients themselves twice daily, detected the LH peak in 33 out of 34 cycles with proven ovulation. In 24 cycles, the LH peak was registered in urine already fourteen hours before it showed in the daily serum controls. The urine LH peak (the test becomes positive at an LH concentration of at least 50 IU/ml) was observed on cycle day 13.6. on average while the corresponding LH peak in serum occurred on cycle day 14.2 on average. The sensitivity of the LH-Colortest was 97.2% and its specificity was 100%.

Adult↗
PubMed1991

Identifying the fertile phase of the human menstrual cycle.

The identification of the human fertile phase as the time during which a woman or a couple may conceive is elusive. The fertile time depends on many factors in each individual menstrual cycle and may be said to be more of a statistical than a physiological entity. This paper reviews the application of statistical methods to three areas related to conception and the fertile phase. The first is the prediction and detection of ovulation from serial measurements, such as hormones, basal body temperature and cervical mucus, throughout the menstrual cycle. Typically, such variables increase from some baseline level to a peak around ovulation (the most fertile time), then subside to low levels in the postovulatory phase. The statistical challenge is to detect the rise (signalling the onset of potential fertility) and subsequent fall. Analytic methods considered include thresholds, Bayesian change-point models and particularly the cumulative sum (cusum) technique which is both simple to apply and understand, and effective. The second area comprises appropriate methods of analysing and interpreting data from clinical studies of the fertile phase, especially in so-called natural family planning (NFP) where it is usual for women to observe several indices of potential fertility. Such studies usually try to establish the temporal relationships between markers of the fertile phase and examine the success of different combinations of markers in delineating the fertile time in comparison with a standard 'defined' phase, for example, the interval from three days before to two days after the peak of luteinizing hormone. The third area is the assessment of the probability of conception on certain days of the cycle, which is vital to the understanding of the fertile phase and its application to NFP. Direct estimation of such probabilities is impractical; instead, resort must be made to estimation by maximum likelihood of the parameters of specially constructed models. Suitable models are described. Finally, the need for a new prospective study of the probability of conception in relation to the markers of the fertile phase used in the symptothermal method of NFP is discussed.

Female↗
PubMed1992

Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus.

Seed transmission of an isolate of pea seed-borne mosaic virus (PSbMV) in several pea genotypes has been studied. Cross-pollination experiments showed that pollen transmission of PSbMV did not occur and accordingly, virus was not detected in pollen grains by ELISA or electron microscopy. Comparative studies between two pea cultivars, one with a high incidence of seed transmission and one with none, showed that PSbMV infected the floral tissues (sepals, petals, anther and carpel) of both cultivars, but was not detected in ovules prior to fertilization. Virus was detected equally well in seed coats of the progeny in both cultivars. Analysis of virus incidence and concentration in pea seeds of different developmental stages demonstrated that in the cultivar with a high incidence of seed transmission, PSbMV directly invaded immature embryos, multiplied in the embryonic tissues and persisted during seed maturation. In contrast, the cultivar without seed transmission did not show invasion of immature embryos by the virus; there was no evidence for virus multiplication or persistence during embryo development and seed maturation. Hence seed transmission of PSbMV resulted from direct invasion of immature pea embryos by the virus and the block to seed transmission in the non-permissive cultivar probably occurred at this step.

Blotting, Northern↗
PubMed1987

Influence of biostimulation by mature bulls on occurrence of puberty in beef heifers.

The objective of this study was to determine if biostimulation of prepuberal beef heifers by mature bulls would alter proportions of heifers exhibiting puberty, or age or weight at puberty. Angus (A), A X Hereford (H) and Tarentaise X HA heifers (n = 103) were stratified by age and weight within breed-type and location of birth and allotted randomly to the following treatments: 1) heifers exposed to mature bulls (T1; n = 52) or 2) heifers isolated from bulls (T2; n = 51). At the start of the experiment, heifers in T1 and T2 were 287 +/- 2 and 286 +/- 2 d of age, respectively. Male-to-female ratio for T1 was 1:26. Heifers in T1 and T2 were maintained in drylots separated by .5 km. Heifers were observed for estrus twice daily for 152 d. Puberty was characterized by the following criteria: 1) behavioral estrus, 2) presence of a palpable corpus luteum (d 9; estrus = d 0) and 3) a rise in serum progesterone above 1 ng/ml (d 9). Proportions of heifers reaching puberty by 11, 12, 13, 14 and 15 mo of age did not differ (P greater than .10) between treatments. Percentages of heifers reaching puberty by the end of the experiment were 84 and 89% for T1 and T2, respectively. Age and weight at puberty did not differ (P greater than .10) between treatments and averaged 370 +/- 7 d and 293 +/- 4 kg, respectively. Results from this experiment indicated that presence of mature bulls did not alter proportions of beef heifers reaching puberty, or age and weight at puberty.

Animals↗
PubMed1998

An electronic nose to detect changes in perineal odors associated with estrus in the cow.

Changes in perineal odor as estrus is approached could form the basis of a new method of estrus detection. Perineal odor of cyclic cows was monitored. Estrus was identified using ovarian ultrasound, behavioral observations, and plasma assay for progesterone and estradiol. Samples were taken from the dorsal lateral perineal (perivulval) area using cotton bud swabs and presented to an electronic nose. Twelve conducting polymer sensors were used to quantify odor in terms of a change in sensor resistance. Preliminary data (Experiment 1) indicate that the odor signals between the luteal phase and estrus could be distinguished for a group of five cows. In Experiment 2, samples were obtained daily from eight cows during the midluteal phase and from d -2 to d 8 of the cycle (d 0 = day of estrus, induced using cloprostenol). Seven of the eight cows cycled normally. Of the 12 sensors, 7 showed a significant change in resistance that was dependent on the day of the estrous cycle. Basal values were those taken in the luteal phase; values peaked on d -1, rose transiently on d 3, and returned to baseline on d 5 to 6. This pattern is strongly correlated with plasma estradiol concentration. The use of artificial olfaction could enable more accurate detection of estrus and has the potential to increase fertility in cows.

Animals↗
PubMed2005

The effect of ovarian follicular fluid and peritoneal fluid on Fallopian tube ciliary beat frequency.

BACKGROUND: The Fallopian tube undergoes well-recognized changes during the ovarian cycle. Ciliary beat frequency (CBF) increases during the secretory phase of the cycle. The stimulus is unknown, although CBF is known to be hormone responsive. At ovulation, follicular fluid is released into the peritoneal cavity and enters the Fallopian tube. We hypothesized that this fluid may provide the stimulus for the increase in CBF detected after ovulation. METHODS: Using a technique which records changes in light intensity, we have studied the effect of pre-ovulatory follicular fluid on CBF of Fallopian tube epithelial cells, and compared this with the effect of either peritoneal fluid or culture medium alone. Follicular fluid samples from 13 women undergoing IVF were collected by selective aspiration of individual follicles. Peritoneal fluid was collected from six women undergoing laparoscopic sterilization. Fallopian tubes were collected from 10 women who underwent hysterectomy for benign conditions. RESULTS: After 24 h incubation, there was a highly significant difference in CBF between the Fallopian tube samples bathed in follicular fluid (mean CBF +/- SEM: 6.34 +/- 0.02 Hz) compared with explants bathed in either medium (4.20 +/- 0.06 Hz) or peritoneal fluid (5.24 +/- 0.03 Hz) (P < 0.005). There was also a significant difference in CBF between tissues bathed in secretory (5.47 +/- 0.03 Hz) compared with proliferative phase peritoneal fluid (4.75 +/- 0.02 Hz) (P < 0.005). CONCLUSIONS: The increase in CBF detected after ovulation may aid ovum pick-up and transport along the Fallopian tube. Factor(s) within human follicular fluid and secretory phase peritoneal fluid may be responsible for this increase in CBF.

Adult↗
PubMed1990

Use of gonadotropin-releasing hormone for hastening ovulation in transitional mares.

Natural GnRH and its analog have potential for hastening ovulation in mares. A study was conducted to evaluate the efficacy of a GnRH agonist given either as an injectable or s.c. implant for induction of ovulation in mares. Forty-five seasonally anestrous mares (March) were assigned to one of three groups (n = 15/group): 1) untreated controls; 2) i.m. injection of the GnRH agonist buserelin at 12-h intervals (40 micrograms/injection for 28 d or until ovulation) and 3) GnRH agonist administered as a s.c. implant (approximately 100 micrograms/24 h for 28 d). Six mares per group were bled on d 0, 7, 14 and 21 after injection or insertion of implant. Samples were taken at -1, -.5 and 0 h and at .5, 1, 1.5, 2, 4, 6 and 8 h after GnRH. Additional daily samples were drawn for 28 d after injection or until ovulation. Samples were assayed for concentration of LH and FSH. Progesterone concentrations were determined in samples collected on d 4, 6 and 10 after ovulation. Number and size of follicles and detection of ovulation were determined by ultrasonography. Number of mares induced to ovulate within 30 d was 0 of 15, 7 of 15 and 9 of 15 for groups 1, 2 and 3, respectively. During treatment, follicle sizes were smaller for mares in group 3 (implant). The LH response to GnRH agonist (area under curve) was similar among groups at d 0 but was greater (P less than .05) for mares in group 3 on d 7 and 14 and groups 2 and 3 on d 21 than for controls. A similar pattern was detected for peak concentrations of LH after GnRH on d 0, 7, 14 and 21. Daily concentrations of LH remained low in untreated control mares compared with GnRH-treated mares throughout the sampling period. Concentrations of LH for mares in group 3 that ovulated were elevated greatly above those for group 2 mares, whereas concentrations of FSH were similar in both treatment groups prior to ovulation.

Anestrus↗
PubMed1990

Comparison of TestPack and Ovustick for predicting ovulation.

A study was designed to compare two methods of predicting the time of ovulation by detection of the urinary luteinizing hormone (LH) surge with two immunoassay kits designed for home use. A newly developed kit, TestPack, was compared with an existing kit, Ovustick. A group of 20 women with regular cycles were studied for one menstrual cycle. Ovulation was established with a serum LH surge and disappearance of the dominant follicle using transvaginal sonography followed by a rise in the serum progesterone level. In the 20 subjects, presumptive evidence of ovulation was obtained by determining the LH surge and the subsequent progesterone peak. In 18 of the 20, disappearance of the dominant follicle was also documented. Ovulation was predicted in 17 of the 20 subjects by both TestPack and Ovustick. Both tests were effective in predicting the day of ovulation, but TestPack provided a more easily defined end point and required less time and effort to perform.

Adolescent↗