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Relationships among dietary roasted soybeans, milk components, and spontaneous oxidized flavor of milk.

Relationships among dietary roasted whole soybeans (RSB), milk fatty acid profile, and the development of spontaneous oxidized flavor of milk were investigated by using 20 commercial dairy herds. Diets contained 0 to 15.3% of dry matter as RSB. Concentrations of dietary RSB were correlated positively with concentrations of C18:2 and C18:3 in milk fat. Concentrations of alpha-tocopherol, beta-carotene, and ascorbic acid in milk decreased from 0 to 3 d of storage (4 degrees C), and oxidized flavor in milk increased linearly between 0 and 8 d of storage. Milk fatty acid profile did not change during storage. The development of oxidized flavor at 8 d postsampling was correlated (r) with increased concentrations in milk fat of C18:2 (0.49), C18:3 (0.55), total polyunsaturated milk fatty acids (0.50), and dietary concentrations of RSB (0.38). Multiple regression was used to quantify relationships between variables and oxidized flavor (samples stored 8 d). All significant models included milk concentrations of Cu and dehydroascorbic acid. Concentrations of C18:2, C18:3, or total polyunsaturated fatty acids in milk fat, or dietary RSB concentrations, and interactions of those variables with Cu were included in individual models. Milk with high concentrations of polyunsaturated fatty acids and Cu were most susceptible to oxidation. Feeding RSB increased polyunsaturated fatty acid concentrations in milk fat, which increased the likelihood of oxidized flavor, especially when milk had high concentrations of Cu.

Animal Feed↗

Noncoagulation of milk in Finnish Ayrshire and Holstein-Friesian cows and effect of herds on milk coagulation ability.

The objectives of this study were to compare milk coagulation ability (MCA) and the prevalence of noncoagulation of milk within the main Finnish dairy breeds, Finnish Ayrshire (FA) and Holstein-Friesian (HOL), as well as to study the herd effect on MCA. Data used in the statistical analyses consisted of individual milk samples of 959 FA, 399 HOL, and 50 crossbred cows from 84 herds. Data were collected before the grazing season in the spring 1999. Milk samples were analyzed for the milk coagulation traits (milk renneting time, R and curd firmness, E(30)) and pH. In addition, information on the 305-d milk production traits from the year 1999, and background information about feeding and management regimes of the herds were obtained. Variance components for the random herd and animal effects were estimated using REML methodology and an animal model. Breed, parity, lactation stage (for R, E(30) and pH only), and a measuring unit (for R and E(30) only) were included as fixed effects in the model. When the effects of concentrate feeding frequency and type of concentrate were studied, the random effect of herd was excluded from the model. A relationship matrix included parents, grandparents, and great grandparents of the cows with observations. The HOL cows were superior to FA cows in MCA when both the proportion of poorly coagulating (PC) and noncoagulating (NC) milk, and the differences in curd firmness were considered. About 30% of the FA cows and 12% of the HOL cows produced PC milk. Only 1.3% of the HOL cows and 8.6% of the FA cows produced NC milk. Herd effect explained only a minor part of the variation in MCA (8%) compared with that in 305-d milk production traits (about 43%). Frequent feeding of the concentrate was associated with good MCA as well as for the high milk, protein and fat yields, but it was not associated with the prevalence of the NC milk.

Animals↗

Computer-controlled milk feeding of group-housed calves: the effect of milk allowance and weaning type.

Calves fed by computer-controlled milk feeders are often weaned gradually by reducing the size of the milk portions. However, reducing the number of milk portions instead may lower calves' occupation of the milk feeder and stimulate their concentrate intake, especially when they are offered a high milk allowance. Before weaning, but not during weaning, the calves on low milk allowance occupied the milk feeder more, consumed more concentrates, and had a lower daily gain. There was no interaction between milk allowance and weaning type. Weaning by reducing the number of milk portions resulted in more unrewarded visits to the milk feeder, but less time ingesting a similar amount of milk. The effect of milk allowance on milk feeder occupancy before weaning was not found when this allowance was halved during weaning.

Animals↗

A survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in pennsylvania.

A 2-part study was conducted to determine the risk of exposure to human pathogens from raw milk. The first part of the study focused on determining raw milk consumption habits of dairy producers. A total of 248 dairy producers from 16 counties in Pennsylvania were surveyed. Overall, 105 (42.3%) of the 248 dairy producers consumed raw milk and 170 (68.5%) of the 248 dairy producers were aware of foodborne pathogens in raw milk. Dairy producers who were not aware of foodborne pathogens in raw milk were 2-fold more likely to consume raw milk compared with dairy producers who were aware of foodborne pathogens. The majority of dairy producers who consumed raw milk indicated that taste (72%) and convenience (60%) were the primary factors for consuming raw milk. Dairy producers who resided on the dairy farm were nearly 3-fold more likely to consume raw milk compared with those who lived elsewhere. In the second part of the study, bulk tank milk from the 248 participating dairy herds was examined for foodborne pathogens. Campylobacter jejuni (2%), Shiga toxin-producing Escherichia coli (2.4%), Listeria monocytogenes (2.8%), Salmonella (6%), and Yersinia enterocolitica (1.2%) were detected in the milk samples. Salmonella isolates were identified as S. enterica serotype Typhimurium (n = 10) and S. enterica serotype Newport (n = 5). Of the 248 bulk tank milk samples, 32 (13%) contained > or = 1 species of bacterial pathogens. The findings of the study could assist in developing farm community-based educational programs on the risks of consuming raw milk.

Animals↗

Bioavailability of iron in goat milk compared with cow milk fed to anemic rats.

Bioavailabilities of iron from dehydrated whole and skim goat milk were investigated using iron-deficient rats. Hemoglobin regeneration efficiencies were determined as the percent conversion of dietary iron into hemoglobin. The respective hemoglobin regeneration efficiencies for groups fed whole goat milk, whole cow milk, skim goat milk, and skim cow milk were 50.6, 13.1, 26.0, and 13.0%, indicating that iron bioavailability of goat milk was greater than cow milk. However, rats fed each milk had negative net increases in hemoglobin concentrations, implying that the iron contents of each milk were not adequate. For animals consuming whole goat milk supplemented with ferrous sulfate, the slope relating hemoglobin iron gained versus iron intake was .95. Respective bioavailabilities relative to ferrous sulfate were 54, 14, 28, and 14% for the four sources of milk. Iron bioavailability of goat milk is superior to cow milk when fed to anemic rats.

Anemia, Hypochromic↗

Triglycerides, fatty acids, sterols, mono- and disaccharides and sugar alcohols in human milk and current types of infant formula milk.

OBJECTIVE: To investigate differences in the fatty acid composition, sterols, minor carbohydrates and sugar alcohols between human and formula milk. DESIGN: We analyzed the concentrations of triglycerides, sterols, di- and monosaccharides and sugar alcohols, as well as the fatty acid composition of 10 currently available types of formula milk for term babies. Results were compared with mature human milk from 99 exclusively breast-feeding Dutch women, who collected 24-hour samples in the second week (n = 99), sixth week (n = 99) and 3 months (n = 25) after delivery. Infant formula milk data were considered different if they fell outside the mean +/- 2s.d. range of corresponding human milk data. RESULTS: The triglyceride concentrations in human milk were lower than those of the formula milk, possibly due to an incomplete collection of fat-rich hindmilk. Formula milks tended towards a higher proportion of medium chain fatty acids and lower proportions of longer-chain polyunsaturated fatty acids. Formulas had cholesterol concentrations 3-35 times lower, and much higher phytosterol concentrations, compared with the human milk. In the formula milk types the glucose, sorbitol and myoinositol concentrations were generally lower, whereas the fucose and erythreitol concentrations were in the lower mean +/- 2s.d. human milk range. The galactose concentrations in the formulas were generally higher. CONCLUSIONS: Formula milk and human milk differ considerably in fatty acid composition and concentrations of cholesterol, phytosterols, monosaccharides and sugar alcohols. The biological consequences of these differences in composition are uncertain.

Carbohydrates↗

Protein composition of rhesus monkey milk: comparison to human milk.

1. Proteins in human milk and Rhesus monkey milk have been compared by FPLC gel filtration and anion exchange chromatography, SDS-Polyacrylamide gel electrophoresis, nitrogen and protein determination. 2. Mature Rhesus milk is higher in protein concentration (15-20 mg/ml) than human milk (8-9 mg/ml). 3. Non-Protein nitrogen is 6-13% in Rhesus milk but 25-30% in human milk. 4. Secretory IgA, lactoferrin, serum albumin, alpha-lactalbumin and lysozyme are present in Rhesus milk, but at a lower concentration than in human milk. 5. The casein subunit pattern is more complex in Rhesus milk compared to human milk. 6. The ratio of whey proteins to casein is similar in both milks (approximately 60/40). 7. A protein with a M(r) of 21,600 is a major component in monkey whey but is not found in human milk.

Animals↗

Induction of cytochrome P450 1A by cow milk-based formula: a comparative study between human milk and formula.

During the treatment of neonatal apnea, formula-fed infants, compared to breastfed infants, show nearly three-fold increase in clearance of caffeine, a substrate of cytochrome P450 1A (CYP1A) and in part CYP3A4. However, human milk is known to contain higher concentrations of environmental pollutants than infant formula, which are potent CYP1A inducers. To gain insight into the mechanism underlying this apparent contradiction, we characterized CYP1A and CYP3A4 induction by human milk and cow milk-based infant formula. The mRNA and protein expression of CYP1A1/1A2 were significantly induced by cow milk-based formula, but not by human milk, in HepG2 cells. Luciferase reporter assay demonstrated that cow milk-based formula but not human milk activated aryl hydrocarbon receptor (AhR) significantly. The cotreatment of 3,4-dimethoxyflavone, an AhR antagonist, abolished the formula-induced CYP1A expression. In addition, AhR activation by dibenzo[a,h]anthracene, a potent AhR agonist, was significantly suppressed by infant formula and even more by human milk. In contrast, CYP3A4 mRNA expression was only mildly induced by formula and human milk. Consistently, neither formula nor human milk substantially activated pregnane X receptor (PXR). Effects of whey and soy protein-based formulas on the AhR-CYP1A and the PXR-CYP3A4 pathways were similar to those of cow milk-based formula. In conclusion, infant formula, but not human milk, enhances in vitro CYP1A expression via an AhR-mediated pathway, providing a potential mechanistic basis for the increased caffeine elimination in formula-fed infants.

Animals↗

Determination of allergenicity to three cow's milk hydrolysates and an amino acid-derived formula in children with cow's milk allergy.

BACKGROUND: Products based on hydrolysed cow milk proteins or amino acid mixtures are recommended in children with cow's milk hypersensitivity. However, some children who are allergic to cow's milk and who clinically react to substitute milk formulas have been observed. OBJECTIVE: To determine the tolerance and allergenicity of protein hydrolysate or amino acid-derived formulas in children with IgE-mediated cow's milk allergy. METHODS: Twenty children with positive cow's milk challenges, positive skin prick tests and/or serum-specific IgE antibodies to cow's milk were selected. Oral challenges, skin prick tests and serum-specific IgE antibodies to extensively hydrolysed whey formula, partially hydrolysed whey formula, extensively hydrolysed casein formula and amino acid-derived formula were performed. RESULTS: Five out of 17 (5/17) children reacted to partially hydrolysed whey formula, (3/16) to extensively hydrolysed whey formula, (2/10) to amino acid-derived formula, (1/16) to extensively hydrolysed casein formula. Only extensively hydrolysed casein formula was tolerated by at least 90% (with 95% confidence intervals) of children. Hydrolysates provoked early and delayed clinical reactions, amino acid mixtures only delayed reactions. Partially hydrolysed whey formula elicited a significantly higher number of positive skin prick test reactions than other formulas. Two children had specific IgE antibodies to extensively hydrolysed whey formula, one to partially hydrolysed whey formula, one to extensively hydrolysed casein formula and none to amino acid-derived formula. CONCLUSION: In this study, none of the cow's milk substitutes has been found to be non-allergenic. Our results suggest that in children with IgE-mediated cow's milk allergy, the first ingestions of extensively hydrolysed cow's milk protein formulas require strict medical supervision because of immediate reactions. This is not the case for amino acid-derived formula. Moreover, our data suggest that treatment of children allergic to cow's milk with cow's milk substitutes should be monitored for several days to document tolerance.

Allergens↗

Compensatory milk production within the bovine udder: effects of short-term non-milking of single quarters.

Daily quarter-milk yields of eight high-yielding cows (24-28 kg/d) and eight heifers (14-24 kg/d) were measured to examined to examined compensatory changes in milk production between quarters within an udder. Either one, two or three quarters per cow were left unmilked for 12 d, in early or late lactation, and then all quarters of all cows were milked normally for a further period of 12 d. Concentrate feeding levels were constant throughout the experiment. The mean daily yield per cow fell to 26, 59, and 75% during the period of milking either one, two or three quarters respectively. Twelve days after resumption of normal milking the total daily yield per cow was the same for cows continuously milked in two, three or all four quarters. Daily yield in cows with only one quarter milked continuously recovered to only 78% of the level of the pretreatment period. During the treatment period, the mean daily yield of the continuously milked quarters increased by almost 14% for cows milked in one quarter only, by more than 10% for two quarters milked, and by 4% per quarter if three quarters were milked. Milk yields of these quarters remained above their pretreatment levels when milking was resumed in the adjacent quarters. There was no difference in the compensatory effect between cows and heifers in early lactation, but the compensatory effects were lower in the lower in late lactation heifers.

Animals↗

Milk yield and mammary growth effects due to increased milking frequency during early lactation.

Increased milking frequency (IMF) at the beginning of lactation has been shown to increase milk yield not only during IMF but also after its cessation. The objectives of this experiment evaluated the effects of increased milking frequency initiated during early lactation on mammary growth and effects on milk yield (MY). Thirty-one cows were divided into treatment groups: 1) 2X: cows milked twice daily (2X) beginning at parturition (d 1), 2) IMF1: cows milked four times daily (4X) from d 1 to 21 postpartum (PP) and 3) IMF4: cows milked 2X d 1 to 3 and 4X d 4 to 21 PP. The 4X cows were milked immediately before 2X cows and again approximately 3 h later, at the end of the normal milking routine. All cows were milked 2X from d 21 to 305 postpartum. Milk yields were 34.5, 37.8 and 37.6 kg/d during wk 1 to 44 for 2X, IMF1 and IMF4, respectively. Mammary biopsies from four cows per treatment were obtained on d 7 and 14 PP to evaluate mammary cell proliferation. Tritiated-thymidine incorporation tended to increase on d 7 in IMF1 cows, and arithmetic means of the percentage of cells expressing Ki-67 proliferation antigen were consistent with a proliferative response to IMF though not significant. Blood was sampled three times per wk during the first 2 wk and then once per wk during wk 3, 4, 5, 6, 8, and 10. Plasma insulin-like growth factor-1 (IGF-1) averaged 20.1 ng/ml in IMF cows vs. 24.2 in 2X but was not accompanied by a change in bST. Prolactin was also not affected by treatment. Neither milk yield nor potential effects on mammary cell proliferation were correlated with systemic IGF-1. Implementing an IMF routine increases MY during treatment and elicits a carryover effect on the remainder of lactation. Milk yield responses after an IMF routine may be the result of increased mammary cell proliferation.

Animals↗

Bone mineral metabolism in full-term infants fed human milk, cow milk-based, and soy-based formulas.

OBJECTIVE: To study the hypothesis that ingestion of a modified soy-based formula with an improved mineral suspension system may result in bone mineral content similar to that observed in infants fed human milk or cow milk-based formulas. DESIGN: Prospective, self-selected group of infants fed human milk randomized between the two formula-fed groups. SETTING: University-based hospital nursery and follow-up. PARTICIPANTS: Fifty-six normal, healthy, full-term infants, free of major malformations or disorders, including 17 infants fed human milk, 19 infants fed a cow milk-based formula, and 20 infants fed a soy protein formula were followed up to 6 months' postnatal age. The soy-based formula studied was modified to improve the suspendability of the minerals. INTERVENTIONS: Infants were fed human milk or the study formula for the first 4 months, at which time beikost was permitted. Infants fed human milk received vitamin supplementation to provide 400 IU of vitamin D per day. MEASUREMENTS: Anthropometric variables, serum calcium, magnesium, phosphorus, alkaline phosphatase, and parathyroid hormone levels were measured at enrollment, and at 8, 16, and 24 to 26 weeks' postnatal age. Bone mineral content at the distal third radius site was measured with single photon absorptiometry at these times. Growth in the infants did not differ significantly among the groups. There was no significant difference in serum calcium, magnesium, alkaline phosphatase, or parathyroid hormone concentrations among the infants during the study. Serum phosphorus was significantly lower at 8 weeks in the group fed human milk than in that fed the cow milk-based formula. Bone mineral content at 16 and 24 to 26 weeks was higher in the group fed the soy-based formula than in that fed human milk, and bone width was also higher at 16 weeks in the infants fed the soy-based formula. CONCLUSIONS: Improving the suspendability of the mineral system in the soy formula results in bone mineralization in infants fed the soy-based formula similar to that measured in infants fed human milk and cow milk-based formula. We suggest that the suspendability of the minerals used is an important variable in the interpretation of the effect of feedings on the bone mineral status of infants.

Animals↗

Feedback control of milk secretion from milk.

Extracellular storage allows biologically-active substances in milk to influence mammary function. Among these factors is one which regulates the rate of milk secretion acutely according to frequency or completeness of milk removal in each mammary gland. The active factor in goat's milk has been identified by screening milk constituents for their ability to inhibit milk constituent secretion in tissue and cell culture bioassays, and found to be a novel milk protein. The proteins identified by bioassy in vitro, also inhibited milk secretion in lactating goats in a reversible, concentration-dependent manner. This protein, termed FIL (feedback inhibitor of lactation), acts by reversible blockade of constitutive secretion in the mammary epithelial cell. As the inhibitor is synthesized in the same epithelial cells, feedback inhibition is, therefore, an autocrine mechanism. FIL's unusual mechanism of action also influences other aspects of mammary function. Acute disruption of mammary membrane trafficking is associated with downregulation of prolactin receptors and followed by a decrease in epithelial cell differentiation. Thus, in addition to acutely-regulating milk secretion, FIL may induce the adaptation in mammary cell differentiation which acts in vivo to sustain the secretory response to a sustained change in milk removal. In the long term, matching of milk output to demand is achieved by a change in mammary cell number. This developmental response is also local in nature. Whether it too is due to autocrine modulation by FIL of mechanisms influencing cell proliferation or survival, or elicited by another milk-borne factor, remains to be determined.

Animals↗

Partitioning of milk accumulation between cisternal and alveolar compartments of the bovine udder: relationship to production loss during once daily milking.

Experiments were undertaken to validate a method (using adrenaline injection) for determination of the size of cisternal and alveolar compartments in the udder, to use this method to determine the pattern of milk accumulation in the udder over time and to determine the relationship between the size of the alveolar and cisternal compartments and tolerance of once daily milking. Cows received intrajugular injections of adrenaline (3 mg) immediately before milking, to block milk ejection and allow harvesting of the cisternal milk fraction. This was followed by removal of the alveolar fraction 30 min later after intrajugular oxytocin (5 i.u.) injection. Results obtained were similar to those obtained by catheter drainage. The alveolar compartment was 90% full at 16 h post milking while the cisternal compartment filled more slowly and was only 70% full at 24 h post milking. At full capacity (measured at 40 h), the volumes of milk contained in the cisternal and alveolar compartments were similar. In a further experiment involving identical twin cows, it was shown that the greater the degree of filling of the cisternal compartment at 24 h, the lower was the production loss on once daily milking. This suggests that the freedom of the alveoli to drain was an important factor in the production loss on once daily milking. Although there were significant correlations within twin sets for milk yield and the size of udder compartments, the relationship within twin sets for yield loss on once daily milking was not statistically significant.

Animals↗

Relative zinc availability in human breast milk, infant formulas, and cow's milk.

The concentration and bioavailability of zinc was analyzed in 10 varieties of milk. The concentration of zinc averaged 4.1 microgram/ml in unprocessed (raw) cow's milk; fortified infant formulas contained about 3 to 5 microgram Zn/ml; human breast milk and processed cow's milk contained approximately 2 microgram Zn/ml; sweetened condensed milk contained 1.3 microgram Zn/ml, and reconstituted nonfat dry milk contained 0.4 microgram Zn/ml. The mean values for zinc bioavailability to rats were as follows: sweetened condensed milk = 66%; human breast milk 59.2%, processed cow's milk = 43.7 to 50.9%; unprocessed (raw) cow's milk = 42%; nonfat dry milk = 41.2%, and infant formulas = 26.8 to 39.5%. Assuming similar absorption of zinc in rats and humans, our experimental results provide some guidelines for estimating the quantity of zinc that would actually be absorbed from various types of milk.

Animals↗

Retention and distribution of iron added to cow's milk and human milk as various salts and chelates.

Iron supplementation of infant formulas is recommended by most national and international organizations, but the optimal form of supplementation has not been determined. We have compared the bioavailability and tissue distribution of iron from four iron chelates and two commonly used iron salts. Weanling C57BL/6J mice were fed for 1 week an evaporated cow's milk diet supplemented with vitamins and minerals (except for iron). Following the adjustment period, mice were divided into 12 groups of 20 each. Six groups continued to receive the cow's milk diet for 18 hours, while the other six groups were fed a similar diet based on human milk. Individual groups received a single dose of milk radioactively labeled with Fe(II)Cl2, Fe(II)SO4, Fe(III)NTA, Fe(III)EDTA, Fe(III)citrate or Fe(III)lactobionate. Wholebody retention was measured after 4 days; animals were then killed and individual tissues were counted for radioactivity. Iron from FeCl2, FeSO4 and FeNTA were the best retained from both milk diets. Fe citrate had a significantly lower iron retention than all other groups in either diet and is probably not an effective chelate for delivering iron to milk diets. Iron bioavailability was higher from the human milk diets than from the cow's milk diets from all vehicles used except citrate and lactobionate. Absorption of Fe citrate was similar from the two milk diets, while percent retention from Fe lactobionate was higher from cow's milk than from human milk. Tissue distribution of retained iron was similar for the milk diets and among the groups, indicating that, once absorbed, iron from the different vehicles is metabolized in a similar manner.

Animals↗

Breast milk jaundice: in vitro inhibition of rat liver bilirubin-uridine diphosphate glucuronyltransferase activity and Z protein-bromosulfophthalein binding by human breast milk.

Twenty-four samples of breast milk from nine mothers of infants suffering from breast milk jaundice were studied. Eight samples of milk from mothers of nonjaundiced infants, along with five formula milks enriched with polyunsaturated fatty acids, served as controls. Milks from mothers with jaundiced infants had no inhibitory effect when assayed immediately after thawing. However, after these milk samples were stores at 4 degrees, they strongly inhibited bilirubin conjugation (80.3% inhibition of uridine diphosphate glucuronyltransferase (UDPGT) activity) and bromosulfophthalein (BSP) binding to cytoplasmic Z protein (dye binding inhibited 82.1%). There was no effect on BSP binding to Y protein (see Table 1). Heating the milk to 56 degrees modified the results in the following manner; when the milk was heated immediately after thawing, no inhibitory effect was seen, even after storage for 96 hr. On the other hand, when the milk was first stored at 96 hr and then heated, it had the same inhibitory effects as the milks which were stored without heating. The present study shows that pathologic breast milk will inhibit BSP-Z protein binding only when stored under conditions that also cause the appearance of the capacity to inhibit bilirubin conjugation in vitro, as well as causing the liberation of nonesterified fatty acids. Thus, the appearance of this inhibitory capacity in vitro seems linked to the lipolytic activity particular to pathologic milks.

Animals↗

Influence of genotype and yield and composition of milk on interval to first postpartum ovulation in milked beef and dairy cows.

Our objectives were to investigate the effects of genotype, yield and composition of milk, and changes in BW and body condition on the initiation of cyclic ovarian activity in nonsuckled beef and dairy cows milked twice daily. Nulliparous heifers were selected from three breeds, 12 Angus, 13 Simmentals, and 26 Holsteins, based on their EBV for milk yield. Cows were machine-milked twice daily, and daily milk yield was recorded; composite samples of milk were collected twice weekly for analyses of milk components. Blood was collected from all cows thrice weekly for 75 d postpartum, and concentrations of progesterone in serum measured by RIA were used to estimate day of first postpartum ovulation. Holstein cows produced more (P less than .05) milk (unadjusted or 3.5% fat-corrected) than Angus or Simmental cows during the first 30 d of lactation. Holstein cows had higher (P less than .01) peak yield of milk than Simmental and Angus cows. Days to peak milk yield were similar for Simmental and Holstein cows, and both were more (P less than .05) than those for Angus cows. Percentages of fat, protein, and total solids in milk were highest (P less than .05) for Simmental cows, whereas milk of Holstein cows had the highest (P less than .05) percentage of lactose and lowest (P less than .05) concentration of somatic cells. Average BW at ovulation differed (P less than .05) among breeds. Estimated daily changes in BW from calving to first ovulation were different (P less than .05) for Holstein and Simmental cows but were similar to those of Angus cows.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗