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Results for “Injections, Intralymphatic”

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At least 271 records · Page 15Linked to original sources
PubMed2002

A new intra-NALT route elicits mucosal and systemic immunity against Moraxella catarrhalis in a mouse challenge model.

Mucosally administered antigens are often poorly immunogenic due to the difficulty of transporting antigens through the mucosal epithelium. We investigated a new route of intranasal-associated lymphoid tissue (intra-NALT) administration of antigens to circumvent the antigen transportation barrier. A comparative study was carried out on mice administered with killed whole cells of Moraxella catarrhalis strain 25238 plus cholera toxin (CT) by intra-NALT injection and nasal inoculation. Both routes induced significant elevations of several isotype antibodies against strain 25238 in saliva, lung lavage, and serum as measured by an enzyme-linked immunosorbent assay (ELISA). Most of these antibodies were paralleled by the numbers of their corresponding antibody forming cells in mucosal or systemic lymphoid tissues. However, intra-NALT injection elicited higher levels of immunoglobulin (Ig) A and IgG in saliva, IgA and IgG in lung lavage, and IgG and IgM in sera than nasal inoculation (P<or=0.05). In addition, both routes generated significant reductions of bacteria in lungs following an aerosol challenge with strain 25238 in a mouse model of pulmonary clearance. Once again, intra-NALT route showed better bacterial clearance in mouse lungs than nasal inoculation (P<0.01). These results demonstrate that intra-NALT administration of antigens is a convenient and effective route for mucosal immunization that elicits improved mucosal and systemic immunity. This new route can be used as a model to study mucosal antigens or vaccine candidates for antigen activation and interaction with the NALT that is one of major inductive sites for common mucosal immune system.

Administration, Intranasal↗
PubMed1991

The effect of intrathymic injection of donor blood on the graft versus host reaction and cardiac allograft survival in the rat.

Our previous paper reported that it was difficult to induce tolerance using donor specific transfusion (DST) in Lewis rats, while in DA and PVG rats it was possible to induce tolerance using DST. In this paper we investigated the effects of DST in a local graft versus host reaction (GVHR) model using these rat strains. The following results were obtained: (i) DST suppressed the GVHR in the DA rat but not in the Lewis rat; (ii) DST did not suppress GVHR in PVG rat strain, but lymphocytes, prepared from the PVG rats that were tolerant of Lewis heart grafts induced by DST, could suppress the the GVHR; and (iii) in Lewis rats, intrathymic injection of whole blood suppressed the GVHR, suggesting this route of antigen delivery might be effective in prolonging allograft survival. Cardiac allograft survival in this strain was prolonged in some cases after pretreatment with intrathymic donor whole blood. These results suggest the value of the GVHR in the assessment of new protocols to induce allograft tolerance in rats.

Animals↗
PubMed2003

Lymphatic mapping with tracer administration into the primary breast cancer.

There is an ongoing debate over the best tracer injection technique in lymphatic mapping for breast cancer. The technique of low tracer volume administration into the primary breast cancer is presented. The reasons that led to this approach are explained as well as its advantages. Excision of radioactivity that remains at the injection site in the breast cancer prevents the gamma ray scatter that may hamper retrieval of a sentinel node. The intralesional injection technique avoids potential injection of tracer fluid across a lymphatic watershed, it enables identification of extra-axillary sentinel nodes and allows probe-guided excision of non-palpable tumours.

Breast Neoplasms↗
PubMed1976

[Improved demonstration of normal lymph nodes in lymphangiograms (author's transl)].

Lymphography with Lipiodol ultra fluid was carried out in eight domestic pigs. The purpose of the experiment was to obtain even distribution of the oil in normal lymph nodes in order to improve its diagnostic value. This was attempted by changes in pressure, temperature and by the addition of benzopyrone. By increasing injection pressure from 0.4 to 0.8 atmospheres, using a pump, it was possible to show more of the lymphatic tissue. Whether this would be possible in man is still not certain. The use of a benzopyrone preparation also improved contrast distribution in lymph nodes. Additional pelvic lymphatics were also seen. The improved distribution of oil is indicated by the altered pattern, strikingly even filling of the vacuoles and 10% incfreased filling rate.

Animals↗
PubMed1973

Skeletal muscle as a privileged site for orthotopic skin allografts.

A semi-privileged status for rat skin allografts may be achieved by placing them on extensive open beds formed by panniculus carnosus muscle which prevents contact of the transplant with host skin. Such allografts enjoy approximately a twofold increase in their life expectancy, even if transplanted across a strong histocompatibility barrier. Experiments are described which rule out stress or a "central" weakening of response, such as enhancement, as explanations of this phenomenon. Intact skin "islands" separated from surrounding host skin on all sides by a broad border of bared panniculus were also found to serve as privileged sites. Dye injected into these islands failed to reach the regional nodes until about the 15th day after their preparation. These studies indicate that a lymphatic deficit is responsible for the observed privileged status of the allografts.

Animals↗
PubMed2003

Endolymphatic infusion: human surgical investigation and application.

The ability to influence changes within the lymphatic system, and to exert a therapeutic influence on the composition and quality of the lymph, may have a substantial impact on the therapeutic outcome in disease interventions. This report concerns our experience with endolymphatic infusion (ELI) of drugs, an approach that we have utilized in a surgical context to destroy bacteria within lymphatic vessels and lymph nodes. Experimentally, we have demonstrated that a variety of antibiotics will temporarily decrease the contractile activity of the lymphatic vessels and reduce the volume of lymph flow. Based on our initial observations, we have applied endolymphatic infusion to clinical care. ELI was evaluated in the treatment of 330 patients, utilizing protocols to alter the characteristics of lymph flow.

Anti-Bacterial Agents↗
PubMed1998

The induction of transplantation tolerance by intrathymic (i.t.) delivery of alloantigen: a critical relationship between i.t. deletion, thymic export of new T cells and the timing of transplantation.

Intrathymic (i.t.) injection of donor alloantigens has proved to be an effective strategy for the induction of tolerance. However, the mechanisms by which tolerance is induced and maintained after transplantation remain unclear. In this report we show that tolerance to donor cardiac allografts can be induced across a MHC class I difference by i.t. injection of donor splenocytes and transient T cell depletion. Furthermore, using H-2K(b)-specific TCR transgenic mice (BM3), we demonstrate that prolonged deletion of donor-reactive thymocytes was essential to induce tolerance by i.t. injection and this was dependent upon donor cells persisting in the thymus. Examination of the kinetics of thymic export following i.t. injection revealed that prolonged deletion of thymocytes was required to delay export of new T cells to the periphery until the time of transplantation. Importantly, after transplantation donor cell persistence in the thymus and i.t. deletion were no longer necessary to maintain tolerance. The graft itself or cells from the graft was responsible for maintaining tolerance at this stage. These findings reveal that multiple mechanisms are responsible for the induction and maintenance phases of tolerance to alloantigens in vivo after i.t. delivery, and that a complex inter-relationship between donor cell persistence in the thymus, i.t. deletion, thymic export of T cells and the timing of transplantation is involved.

Animals↗
PubMed1999

Regulation of NOD mouse autoimmune diabetes by T cells that recognize a TCR CDR3 peptide.

NOD mice spontaneously develop type I diabetes resulting from autoimmune destruction of their insulin-producing beta cells. Among the self-antigens targeted by NOD autoimmune T cells is a peptide, p277, from the sequence of the 60 kDa heat shock protein (hsp60). Common to the anti-p277 T cell populations of NOD mice is an idiotope, C9, that spans the CDR3 region of the C9 TCR. We now report: (i) that the C9 idiotope peptide can be presented directly to anti-C9 anti-idiotypic T cells by C9 T cells, (ii) that spontaneous anti-C9 anti-idiotypic T cell activity falls as disease progresses, but immunization can activate the anti-idiotypic T cells to regulate the autoimmune process, (iii) that the anti-idiotypic T cells secrete IFN-gamma, but appear to control the disease by down-regulating the IFN-gamma produced by the pathogenic population of anti-p277 T cells, (iv) that intrathymic administration of the C9 idiotope peptide at 1 week of age can accelerate the disease, and (v) that administering the p277 target peptide can up-regulate the anti-idiotypic T cells and arrest the disease process. Thus, the development of NOD diabetes can be regulated by a balance between anti-idiotypic and anti-target peptide autoimmunity, and anti-idiotypic regulation can lead to changes in the cytokine secretion of the autoimmune T cells involved in the disease process.

Amino Acid Sequence↗