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[Demonstration of plasminogen activators in the saliva of a predatory insect of the family of Reduviidae (Heteroptera)].

The study of digestive and salivary proteases of Rhapactor biparticeps, has shown that the salivary glands of this insect are rich in proteolytic enzymes, however, the gut has none. The characterisation of these enzymes on different substrates showed that they are only active on fibrinogen and plasmatic proteins which are involved in the coagulation system. Among these fibrinolytic enzymes that has been identified and characterized, are the plasminogen activators. Their unexpected presence in the entomophagic insect is discussed.

Animals↗

Histological and histochemical studies on the ovaries of the red cotton bug, Dysdercus koenigii (Heteroptera: Pyrrhocoridae).

A histological and histochemical study of the ovary of Dysdercus koenigii has been carried out to ascertain the origin of protein, lipid and carbohydrate components of the yolk during vitellogenesis. Germaria of the telotrophic ovarioles of this insect comprise mononucleate and binucleate trophocytes, prefollicular cells and oogonia ensheathed in two membranes. The follicular epithelium derived from the prefollicular cells is multilayered in the beginning of the oocyte development but is progressively reduced to one layer in later stages. Chromatin material in the binucleate trophocytes condenses to form DNA-positive blobs. RNA-positive material flowing from the nutritive cords can be seen at the nutritive cord-oocyte junction. Protein bodies appear first in the intercellular spaces of follicular epithelium during the early vitellogenic stages indicating the supply of yolk protein from the haemolymph. Lipids originate as L1 bodies made up of unsaturated phospholipids in the nucleus (germinal vesicle). These bodies by addition of saturated triglycerides from haemolymph change to L2 bodies, which in turn, knock off phospholipid part and change of L3 bodies. Polysaccharides come as extrusions from the germinal vesicle while glycogen is first seen in the intercellular spaces of follicular epithelium and then passes into the oocyte indicating its origin in the haemolymph.

Animals↗

Histochemical observations on the neuroendocrine complex of Heteroptera, II--The endocrine glands.

A histochemical analysis of the retrocerebral endocrine glands of four heteropteran bugs, viz. Sphderodema rusticum Fabr., Ranatra filiformis Fabr., Enithares indica Fabr. and Spilostethus pandurus has been made. Histochemical tests for various types of carbohydrates, proteins, lipids, nucleic acids, etc. have been made. The reaction of different types of cells of the endocrine glands viz. chromophil, chromophobe and CA cells have been studied.

Animals↗

Neurosecretion in the milkweed bug, Lygaeus pandurus scop. (Heteroptera: Lygaeidae).

Neurosecretory cells in the brain, ventral ganglia, and retrocerebral endocrine glands aortal complex of a milkweed bug, Lygaeus pandurus Scop. have been described using a variety of techniques in whole mounts and sections. Two main types of neurosecretory cells viz. A and B types, have been distinguished on the basis of histochemical and staining properties. The A-type of cells have been further divided into 4-sub types viz. A-1, A-2, A-3 and A-4 on the basis of staining properties and cell size. There are only one type of B-cells. The brain and all the ventral ganglia contain neurosecretory cells including the frontal ganglion. The brain consists of A-1, A-2 and B-cells, the suboesophageal and prothoracic ganglia have A-2, A-3 and B-cells, and the lat ganglion have A-2 and B-cells. The frontal ganglion contains only A-4 type of cells. The corpora cardiaca are ovoid bodies consisting of large and small chromophil cells. The chromophil cells are positive to PF and PARF but negative to PAVB. In addition to the secretions of the chromophil cells, the gland also acts as storage organ for lateral, ventral, posterior and anterior neurosecretions of the brain. The corpus allatum which is an unpaired globular epitheloid body, lies behind the corpora cardiaca. It consists of one type of cells. The gland is devoid of A-1 cell NSM i.e. Victoria blue positive material. At times, it shows a small amount of A-2 cell NSM. The aorta which receives the axons of NCC-I is provided with a large amount of neurosecretory material, elaborated in the medial neurosecretory cells of the brain. Thus, in Lygaeus pandurus aorta and not the CC, functions as NHO for medial neurosecretions.

Animals↗

Effect of nutritional status and sex on the circadian variation in predation and haemolymph free sugars of Diplonychus indicus n. sp. (Heteroptera: Belostomatidae).

Total free sugars in the haemolymph of an aquatic hemipteran, Diplonychus indicus n. sp. were analyzed at different times of day under differential nutritional conditions. Feeding was observed to mask the time of day variation in the haemolymph free sugars. Predatory periodicity during the day differs between sexes, suggesting the tendency of these insects to avoid intra-specific competition for food.

Animals↗

Neuroendocrine system and storage of neurosecretory material in the dorsal aorta in a bug Chrysocoris stollii Wolf (Heteroptera: Pentatomidae).

The neuroendocrine system of Chrysocoris stollii has been described with the help of PF bulk-stained preparations and sections. Two groups of median neurosecretory cells (NSC), each consisting of 5 A-cells in females and 7 A-cells in male individuals together with 3 B-cells, have been observed in the pars intercerebralis medialis of the brain. The lateral neurosecretory cells are absent. The axons of the two groups of median neurosecretory cells give off two bundles of neurosecretory axonal pathways which decussate in the anterodorsal part of the protocerebrum. From the point of decussation the two pathways run deep into the protocerebrum and deutocerebrum and emerge from the tritocerebrum as nervi corporis I (NCC I). The NCC I of both the sides enter into the aorta wall. The NSM is present in the aorta wall only, which comes from the bain through the NCC I. The nervi corporis cardiaci II (NCC II) are two short nerves originating from the tritocerebrum and entering the corpora cardiaca of its side. Two posteriorly fused oval corpora cardiaca are present below the aorta and dorsal to the oesophagus. A single semicircular corpus allatum is present behind the corpora cardiaca. Two oval A-type neurosecretory cells are present in the suboesophageal ganglion whereas other ganglia of the nerve cord lack the NSC. The aorta acts as a neurohaemal organ in this insect.

Animals↗

[Demonstration of an enzyme inhibiting coagulation of blood ingested by the blood-sucking insect, Rhodnius prolixus (Heteroptera, Reduviidae)].

A new fibrinolytic enzyme, "the prolixase", was isolated and purified from the haemolymph of Rhodnius Prolixus. The mode of action of the enzyme was studied and compared to that of extracts from salivary glands and midgut of the insect. It appears that the prolixase is involved in inhibiting the coagulation of the blood ingested by Rhodnius during its blood meal. The mode of action of the enzyme in this phenomenon is discussed.

Animals↗

Histo-physiology of the salivary glands of the red cotton bug, Dysdercus koenigii (Pyrrhocoridae-Heteroptera)--histological, histochemical, autoradiographic and electron-microscopic studies.

1. Gross morphology, histology, histochemistry and ultrastructure of the salivary glands of Dysdercus koenigii have been studied. The mode of origin of saliva was investigated by tracer techniques. 2. Differences in histological structure of the glandular lobules, consistency and staining properties of the lumen contents, as also in their capability to incorporate materials from haemolymph have been observed, pointing to a possible division of labour among the different parts of the gland. 3. 3H-thymidine experiments showed that some of the nuclei of the gland cells incorporate the label asynchronously, indicating their polyploid growth or repair DNA synthesis or even specific gene amplification. 4. 3H-uridine incorporation patterns showed that the gland cell nuclei are the principal centres of RNA synthesis. The synthetic products move into the cytoplasm and then into the glandular lumen. The RNA exported into the lumen conjugates with the protein to form RNP which provides the material for the formation of the protective sheath around the mandibular, and maxillary stylets at the time of piercing and sucking. 5. Autoradiographic experiments with 3H-L-histidine showed that the site of heavy labelling shifts from outside inwards with progressive incubation times, indicating that the glandular epithelium is capable of transporting haemolymph materials (proteins?) into the lumen. Some of the haemolymph protein fractions could possibly be used as the precursors of salivary components, in addition to the endogenously synthesised materials. 6. Salivary histochemical studies revealed the presence of glycogen both in the lumen and in the granular epithelium. 3H-glucose labelling patterns are in agreement with the histochemical localization of glycogen and suggest that the blood sugar (trehalose) might be appropriated by the gland cell epithelium for the formation of glycoproteins. 7. Ultrastructural observations of the gland cells showed that the basal borders of the cells are highly folded to form pits. Pinocytotic uptake of the haemolymph materials may be facilitated by these pit formations. The apical borders of the cells facing the lumen develop numerous microvilli and the secretion granules escape into the lumen by pinocytosis, as revealed also by the autoradiographic studies with tritiated histidine. The distribution of golgi vesicles, mitochondria, microtubules is not uniform. They tend to show a higher density towards the apical border than towards the basal border of the cells. This difference may be related to the well-known functions of these organelles in packing the secretion products and to provide energy and channels for their transport. The rER exists in the form of numerous vesicles scattered evenly throughout the cytoplasmic space. But they also exist as stacks of membranes. Their proximity with golgi vesicles seems to be significant for the packing process of the secretion products.

Animals↗

Proline metabolism in heteroptera from different environment.

Free amino acids composition of six Heteropteran insects belonging to aquatic and terrestral habitate has been observed and it is suggested that the distribution of proline is related to the habitate of these insects. Proline probably plays a role in the retention/conservation of the water in terrestrial insects.

Amino Acids↗