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PubMed2005

Internal labelling of the eggs of the aphidophagous hoverfly Episyrphus balteatus (De Geer) (Diptera: Syrphidae) with a fluorescent dye.

A method was developed to label the egg load of Episyrphus balteatus (De Geer) (Diptera: Syrphidae) by feeding adult females with the fluorescent dye Rhodamine B. In a preliminary experiment aimed at selecting an appropriate dye concentration, gravid females reared in insectary conditions were provided for two successive days with a 50% honey-water solution enriched with Rhodamine B at concentrations of 0.1%, 0.01% and 0.001%. Eggs deposited on aphid-infested broad bean plants by syrphid females fed with Rhodamine B 0.01% showed the most intense marking. The higher dye concentration induced irregular marking among the eggs, presumably due to its repellent taste and consequently lower intake by the flies. A second series of trials focused on the persistence of the marker, prior and after oviposition, at the selected (optimal) dilution. A reduction in the marking degree of eggs was observed when the first egg deposition was delayed for one day after removing the dye from the cage. When marked females were allowed to deposit further batches of eggs the subsequent days, fewer eggs were laid and their fluorescence properties was reduced. Exposure to direct sunlight during 10 hours had no detectable effect on the fluorescence level of marked eggs deposited on a bean plant. The egg-marking technique examined in this study could be useful to determine the crop area accommodating eggs deposited by marked syrphid females migrating from a release point into cultivated fields.

Animals↗
PubMed1999

[The influence of diptera on the domestic animals].

The Diptera attacked domestic animals in Poland are: Simuliidae, Culicidae, Tabanidae, Gasterophilidae, Hypoder-matidae and Muscidae. The insect assail the crazing animals, mostly during sunny day dna at close of day. Lack of effective repelents and also difficulties with their usage, makes it impossible to complete extermination of parasitic insects in the environment. It is posible to effectively fight against Hypoderma bovis and Gasterophilus spp.

Animals↗
PubMed1991

The retrocerebral endocrine complex in Diptera.

The retrocerebral endocrine glands were studied in 20 insects belonging to the three suborders of Diptera. The CC-CA complex either takes the form of a ring gland, or consists of paired CC and CA. In the suborders Nematocera and Brachycera, both types of organization are to be seen, but only ring gland organization is present in members of the suborder Cyclorrhapha. NCC I and NCC II are found only in insects with paired CC and CA. The histology of the CC and CA is described. NCA1 is clearly discernible in species with paired organization. NCA2 was seen in only one species (Tabanus speciosus), but no swelling was observed on this nerve. NAA and NAO were not present in any of the 20 species studied.

Animals↗
PubMed1989

Canavanine incorporation into the antibacterial proteins of the fly, Phormia terranovae (Diptera), and its effect on biological activity.

In response to microbial infection or mechanical injury, larvae of the fly, Phormia terranovae (Diptera), can induce de novo production of a group of antibacterial proteins including: peak I protein, diptericin A, diptericin B, diptericin C, and peak V protein. Administration of L-canavanine at the time of mechanical injury results in the incorporation of this arginine antagonist into these proteins. Canavanine replacement for arginine causes a total loss of detectable antibacterial activity for diptericin B and diptericin C, whereas diptericin A and peak V protein are severely inhibited. This loss in biological activity occurs in spite of the fact that canavanine stimulates induced protein synthesis. Analysis of the hydrolysate of diptericin A reveals that one-third of the 3 arginyl residues are replaced by canavanine. This investigation provides the first evidence that canavanine incorporation into a protein can impair its function.

Amino Acids↗
PubMedDate not supplied

[Effect of the Pechenezhskiy reservoir on species distribution and number of blood-sucking Diptera].

Studies of the fauna, density and specificity of formation of the blood-sucking Diptera habitats have demonstrated rapid reduction of biotopes and the density of insects in the first years after the construction of the reservoir. In the following years aggravation of the entomological situation was observed, relative stability being attained only by the 20th year of the reservoir' functioning. Further increase in anthropogenic impact may lead to entomologic changes in the area.

Animals↗
PubMedDate not supplied

[Ultrafine structure of the malpighian tubules of hematophagous Diptera].

The ultrastructure of Malpighian tubes of 5 species of bloodsucking Diptera was studied: Culicoides pulicaris, Tabanus bromius, Hybomitra schineri, Haematopota pluvialis and Stomoxys calcitrans. The Malpighian tubes of the above species include the cells of two types. The most abundant cells of the 1st type contain many spherical inclusions which represent deposits of mineral compounds. The microvilli of the 1st type cells always contain mitochondria. Cells of the 2nd type are characterized by a smaller size, their microvilli lack mitochondria and no sphere crystals are observed in cytoplasm. Differences in the ultrastructure of epithelial cells of Malpighian tubes were found out.

Animals↗
PubMed1979

[The primary structure of a dimeric hemoglobin (erythrocruorin); component CTT VI of Chironomus thummi thummi, Diptera (author's transl)].

The complete amino acid sequence of 147 residues was determined automatically for a major dimeric component (CTT VI) of the insect larva Chironomus thummi thummi (Diptera). The molweight was found to be 32411. All tryptic, maleylated tryptic and cyanogen bromide peptides were isolated. The handling of some large fragments was facilitated by maleylation and subsequent ion exchange chromatography. Some details of the primary structure are discussed. The alignment of the amino acid sequence with that of human alpha-chains shows only 29 identical positions.

Amino Acid Sequence↗
PubMed1978

[Infestation of roe deer by Cephenemyia stimulator (Clark) (Diptera, Oestridae) in Trent province].

Cephenemya stimulator (Clark) (Diptera Oestridae) is first recorded in Italy parasiting roe deer (Capreolus capreolus). Diagnosis was made by examination of II-III instar larvae (fig. 1-2) from roe deers killed in Trient province. In these last years in the Trentin region, there was an increasing of roe deers number (actually about 20.000); at the same time growed roe deers infestation by C. stimulator (Tab. 1). Females of C. stimulator probably attack young roe-deers lying down on field-pastures near forest (fig. 3). From examination of killed roe-deers (Tab. 2) there is evidence of debilitation status of infested specimens. Location and dislocations of parasitising larvae in the head cavity was that shown in fig. 4 and 5.

Animal Diseases↗
PubMedDate not supplied

[Parasitic effect of Opius concolor (Spzl) (Hymenoptera, Braconidae) on an intermediate host Ceratitis capitata Wied (Diptera, Trypetidae)].

The parasitic incidence of Opius concolor (Hymenoptera, Braconidae) on a replacement host, Ceratitis capitata (Diptera, Trypetidae) was studied using polyacrylamide gel electrophoresis and immunoelectrophoresis methods. A noticeable modification of C. capitata proteins was observed when parasited by O. concolor. But the most important phenomenon is the finding of common antigens between the host and the parasite. These results are discussed with regard to trophic and parasitic behaviour of the parasite.

Animals↗
PubMed1980

Widespread IgE-mediated hypersensitivity in the Sudan to the 'green nimitti' midge, Cladotanytarsus lewisi (Diptera: Chironomidae) II. Identification of a major allergen.

A major allergen has been identified in an aqueous extract of the 'green nimitti' midge, Cladotanytarsus lewisi (Diptera: Chironomidae). Following chromatography on Sephadex G-100 allergenic activity, as assessed by skin ('prick') testing, eluted as two closely related peaks (pools I and II) at about 50% bed volume. When these pools were applied separately to columns of CM-cellulose, activity in each eluted with 0 . 05 M NaCl. Isoelectric focusing of the unfractionated allergen gave a single peak of activity at pI 4 . 3. By SDS--PAGE, biological activity in the whole 'green nimitti' extract and the material eluting from both pools I and II of the Sephadex G-100 column migrated to the same positions and were associated with a molecular size of 15,000--20,000 daltons. Skin test reactivity of the unfractionated material and the Sephadex G-100 pool I and II eluates were all destroyed following incubation with trypsin, chymotrypsin, thermolysin and neuraminidase. These experiments indicate that a major allergen derived from the 'green nimitti' midge, a cause of widespread and severe immediate-type allergy in the Sudan, is an acidic glycoprotein of 15,000--20,000 molecular weight.

Allergens↗
PubMed1980

Urogenital myiasis caused by Chrysomya bezziana (Diptera: Calliphoridae) in peninsular Malaysia.

A case of urogenital myiasis caused by Chrysomya bezziana (Diptera: Calliphoridae) was diagnosed in a 76-year old patient who had carcinoma of the rectum. A total of 35 larvae were obtained from ulcers near the external genitalia and urethra opening. Larvae pupated within 1 to 2 days and 6 days later emerged as adult males. These were identified as Chrysomya bezziana. Female flies possibly attracted by the fetid odour, laid eggs in the existing lesions in the urogenital area, the larvae invading and feeding on living tissue. Lack of personal hygiene was the contributing factor for the cause of urogenital myiasis in this patient.

Aged↗
PubMed1994

Preliminary observations of the effects of phencyclidine in decomposing tissues on the development of Parasarcophaga ruficornis (Diptera: Sarcophagidae).

Larvae of Parasarcophaga ruficornis (Fabricius) (Diptera: Sarcophagidae) were reared on tissues from rabbits administered different dosages of phencyclidine to study the effects of this drug on the development of this insect species. The rabbits were given 3.66, 7.31, and 14.62 mg of phencyclidine via ear vein infusion. No significant differences in larval growth rate were observed among the colonies, although the duration of the non-feeding portion of the third instar was shorter for larvae fed on tissues containing the drug. Mortality during the larval stage was directly related to the dosage of phencyclidine administered, ranging from 0 in the control colony to 29.0% in the colony fed on tissues from the rabbit receiving 14.62 mg of phencyclidine. Durations of the puparial stage were longer for colonies fed on tissues containing the drug. Presence of phencyclidine was detected in larvae from all colonies fed on tissues from rabbits receiving the phencyclidine using GC/MS.

Animals↗
PubMed1994

The black soldier fly Hermetia illucens (Diptera: Stratiomyidae) as a potential measure of human postmortem interval: observations and case histories.

The black soldier fly, Hermetia illucens (L.), has been shown to be a ubiquitous inhabitant of both surface and buried human remains throughout the southern, central and western United States and Hawaii. Unlike most other species of forensically important Diptera, this species frequently dominates bodies in the dry/post decay stage of decomposition. Adults of the black soldier fly appear to initiate oviposition (egg laying) 20 to 30 days postmortem. Even at warm temperatures (27.8 degrees C), subsequent completion of the life cycle can require an additional 55 days. Life history data for H. illucens, when used in combination with data for other cohabiting arthropod species and viewed in the context of local environmental conditions, can provide medicolegal investigators with valuable parameters for estimating the postmortem intervals for badly decomposed remains.

Animals↗
PubMedDate not supplied

[Host range of drosophila melanogaster C virus among diptera and lepidoptera (author's transl)].

The host range of the C picornavirus of Drosophila melanogaster was studied, using numerous strains of Drosophila together with four other genera of diptera and two species of lepidoptera. C virus was injected into the different hosts and serially passaged in them. The extracts from each passage were biologically assayed on virus free D. melanogaster. Four different situations were found. 1) A high level of multiplication leading, in 45 strains of Drosophilidae, to the early death of the hosts. This phenomenon was particularly related to the subgroup melanogaster and in the dipteron Ceratitis capitata. The titre of the virus in this latter insect was high as in D. melanogaster, but its cellular tropism was wider. 2) An active viral multiplication but without symptoms, in two strains of D. immigrans and in the lepidopteron Galleria mellonella. In these two insects, the viral titre was clearly inferior to that observed in D. melanogaster. 3) Maintenance of the virus, without multiplication in the dipteron Calliphora erythrocephala and the lepidopteron Arctia caja. In these insects, the decrease in virus titre was directly related to the dilution factor at each passage. 4) Rapid disappearance of the virus, in the mosquitoes Culex pipiens and Aedes aegypti. The host range of C virus is compared to that of Sigma virus of Drosophila and of two other picornaviruses of insects.

Animals↗
PubMed1993

Occurrence and prevalence of bot flies, Metacuterebra apicalis (Diptera: Cuterebridae), in rodents of cerrado from central Brazil.

The occurrence and prevalence of Metacuterebra apicalis (Diptera: Cuterebridae) in natural populations of Oryzomys subflavus, Bolomys lasiurus, and Thalpomys cerradensis (Rodentia: Muridae) from August 1990 to July 1992 in the cerrado, a common savanna-like vegetation type of central Brazil, are reported. An increase in bot infection in the 3 rodent species between October 1991 and July 1992 without correlation to precipitation was detected. The prevalence was lower than in neotropical forest formations. Mean intensity was 1.3 bots (range 1-2) for T. cerradensis and 1 bot for B. lasiurus and O. subflavus. This is the first record of T. cerradensis as host of bot flies.

Animals↗
PubMed1993

Preliminary observations of the effects of amitriptyline in decomposing tissues on the development of Parasarcophaga ruficornis (Diptera: Sarcophagidae) and implications of this effect to estimation of postmortem interval.

Larvae of Parasarcophaga ruficornis (Fabricius) (Diptera: Sarcophagidae) were reared on tissues from rabbits administered different dosages of amitriptyline to study the effects of this drug on the development of this insect species. The rabbits were given 300, 600, and 1000 mg of amitriptyline via ear vein infusion. No significant differences in rates of larval growth were observed among the colonies. Durations of the larval stage were significantly longer for larvae fed on tissues from rabbits receiving amitriptyline. Larval mortality was observed to be 5.5% for the control colony, but ranged from 40.5 to 57.5% for the test colonies. Durations of the puparial stage were significantly longer for the colonies fed on tissues from the rabbits receiving the 600 and 1000 mg dosages of amitriptyline than for the control and colony fed on tissues from the rabbit receiving the 300 mg dosage. Observed differences in the durations of the larval and pupal stages from the test colonies were sufficient to alter a postmortem interval estimate by up to 77 h, if based on normal developmental patterns for this species at 26 degrees C. Presence of amitriptyline and nortriptyline could be detected in larvae from all colonies fed on tissues from the rabbits receiving amitriptyline using high-performance liquid chromatography (HPLC).

Amitriptyline↗
PubMed1995

[The importance of flies (Diptera-Brachycera) in the dissemination of helminth eggs from sewage treatment plants].

Screenings were carried out in four mechanico-biological municipal wastewater treatment plants in two climatico-geographic regions of Slovakia (submontane region: Levoca, Poprad and Tatranská Lomnica, lowland region: Michalovce). Ovoscopical studies traced the occurrence of helminth eggs in stabilized sludges in sludge beds. The flies (Diptera-Brachycera) are considered as potential biological vectors of helminth eggs. The stabilized sludges in sludge beds showed the presence of a wide range of helminth eggs Ascaris sp. 0-184 spec. Hymenolepis sp. 0-5 spec. Toxocara sp. 0-17 spec. Taenia sp. 0-2 spec. Trichuris sp. 0-17 spec. a Capillaria sp. 0-4 spec./100 g of sample dry matter. In the areas of four sewage treatment plants 461 fly specimens (358 females, 103 males) were captured, belonging to 31 species. On three specimens of Protophormia terraenovae ectoparasites (mites) were detected. No eggs were found on the body surface of the flies.

Animals↗