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PubMed1989

Early morphogenesis of the canine lens capsule, tunica vasculosa lentis posterior, and anterior vitreous body. A transmission electron microscopic study.

This study provides transmission electron microscopic observations on the early morphogenesis (from days 25-35 post coitum) of the canine posterior lens capsule, the tunica vasculosa lentis (TVL) posterior and the anterior part of the vitreous body. The presence of an anlage of the posterior lens capsule as early as day 25, recently described histologically, was confirmed by this study. In the period from day 25 to day 35, the polar part of the posterior lens capsule develops 2-29 continuous and parallel lamellae, matching 50 nm and 1.74 microns, respectively. At these early stages, the TVL consists of capillaries that are simple endothelial tubes. From day 28 onward, these can be classified as A-1-alpha capillaries according to the classification of Bennett et al. [3]. In direct proximity to the lens capsule, the vitreous body contains fibrillar material with a morphological appearance similar to that of the lens capsule. This material probably derives from both the capillary endothelial cells' basal lamina and the lens capsule. Only few cellular components were observed in the anterior vitreous body. The development of the described structures is grossly in accordance with that observed in other mammalian species. The observations presented serve as a reference for studies on the pathogenesis of persistent hyperplastic tunica vasculosa lentis/persistent hyperplastic primary vitreous (PHTVL/PHPV), which is an important cause of leucocoria in children and in some dog breeds.

Animals↗

PubMed1992

[Observations during captive bolt stunning of rabbits].

78 rabbits of mixed breed and about 3 kg live weight were stunned before slaughter with a commercial spring operated captive bolt apparatus designed for rabbits and water fowl. The following reaction patterns were observed: 1.) Immediate onset of tonic spasm, followed by weak to heavy clonic spasms and/or subsequent relaxation; irreversible loss of corneal reflex and cessation of respiration: effective and irreversible stun tantamount to killing 2.) Same reaction as before except that respiration restarted after 1/2 to 2 min: effective but only temporary stun 3.) Similar reaction as before, but respiration maintained: insufficient stun 4.) Immediate onset of weak tonic spasm; respiration and corneal reflex maintained: insufficient stun. According to this classification 56 rabbits (72%) were killed outright and 18 (23%) temporarily stunned while in 4 (5%) the stun was ineffective. The captive bolt apparatus proved thus to be principally suited for the stunning resp. killing of slaughter rabbits. The best stunning results were obtained with shots into the parietal bone near the sagittal line but not hitting the bone sutures. To achieve this the apparatus has to be placed slightly paramedian on the front as close to the ears as possible. Insufficient stunning results could be blamed on deviating shooting positions. To avoid misses a good fixation of the animal including its head is necessary. Correct application provided the use of penetrating concussion stunners should be preferred to applying a blow to the neck for stunning rabbits.

Abattoirs↗

PubMed2003

Yield stability in Coffea canephora from diallel mating designs monitored for 14 years.

The genetic parameters of several agronomic traits were estimated in Coffea canephora in a triangular diallel with six parents and in a partial diallel with 18 parents. The yield data came from harvests obtained over 14 years divided into an initial 6-year cycle, a second cycle of 5 years after cutting back and a third cycle of 3 years after topping. Risk incidence was also observed over several years. The general combining abilities (GCA) were the principal sources of variation for the various traits considered. Parents derived from the Yangambi selections (Democratic Republic of Congo) proved to be best for yields in the three cropping cycles. The rank correlations between the GCA of the various traits indicated that first-cycle production was still not enough to predict the GCA for yield over 14 years. Susceptibility to leaf anthracnose and to drought could lead to modifications in successive classifications, with a more significant impact on production during the first cycle. The differences in susceptibility to Coffee Berry Borers had an impact on yield, which increased in line with coffee tree age. There was a rather good relation between inherent values and values in parent combinations and it was thus possible to choose parents on their own performance.

Africa South of the Sahara↗

PubMed1995

Molecular basis of human hypertension: the role of angiotensin.

On the basis of recent advances in molecular biology and statistical genetics, it has become possible to search for chromosome regions that contain genes predisposing to hypertension and to directly link specific mutations on candidate genes to hypertension. As the human genome has been extensively mapped, highly informative, polymorphic markers are available, which can be used to detect genes in their proximity with 'hypertensinogenic' alleles. Some of these markers have been shown to be tightly linked to the genes of the renin-angiotensin system. Furthermore, the coding and regulatory regions of the genes encoding for renin, ACE, angiotensinogen and the AT1 receptor have been partially characterized. This provides a basis for further definition of specific polymorphisms within these genes that are of functional importance and that can be used to examine their contribution to the inheritance of primary hypertension. The first studies of these links have already emerged and have been reviewed in this article. Several problems arise in performing such linkage studies in human primary hypertension, however. It is difficult to define the genetic background of heterogeneous, multigenetic and multifactorial diseases such as human hypertension. Extensive studies of population genetics, including the analysis of large numbers of generations and controlled breeding experiments, cannot be performed, for obvious reasons. Blood pressure is not a convenient study trait, because it exhibits great intraindividual variance and also because of the relatively low reliability of just a few indirect measurements obtained under loosely controlled environmental conditions. Twenty-four-hour ambulatory blood pressure measurements may improve such investigations in the near future. Ravogli et al (1990) reported that the 24-hour ambulatory systolic blood pressure is higher in normotensive subjects of hypertensive parents than in normotensive subjects of normotensive parents--a finding that had not been previously reported using the conventional method of measurement. Hypertension as a trait per se is also problematic: its classification (above 140/90 mmHg) is purely artefactual, and its aetiology is highly heterogeneous. Thus, we have to keep in mind that even strong gene effects, if present in only a small subgroup of hypertensives, may not be detected in these studies. Attempts are being made to strengthen the analysis by characterizing physiologically distinct subgroups. In addition, the investigation of intermediate phenotypes, such as plasma parameters, which are more reliable and less subject to variations, may be helpful.(ABSTRACT TRUNCATED AT 400 WORDS)

Angiotensin II↗

PubMed2006

Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain.

Small ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two). Animals (one per flock) belonged to distantly located, single-species flocks (goat or sheep). Sequence analysis showed conservation of regions that are putatively relevant to viral survival. Sequences of Spanish goat and sheep SRLV were allocated into phylogenetic trees (phylograms) with known SRLV groups. The phylograms corresponding to the pol, gag-p25 and LTR regions analyzed presented a compatible topology. This showed that Spanish caprine and ovine SRLV sequences belonged to the A or D phylogenetic groups and were closer to sheep SRLV prototypes (A1 group) than to goat SRLV prototypes (B or C groups), according to the current classification [Shah, C., Boni, J., Huder, J.B., Vogt, H.R., Muhlherr, J., Zanoni, R., Miserez, R., Lutz, H., Schupbach, J., 2004a. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology 319 (1), 12-26]. It was not possible to amplify in the three genetic regions the expected fragment in additional Spanish caprine and ovine SRLV proviral DNA sequences with the PCR primers used. This suggests that there is heterogeneity at the primer binding site among Spanish SRLV sequences. It also illustrates the need to develop diagnostic tests that are sensitive in local breeds.

Amino Acid Sequence↗

PubMed1988

Measuring genetic change in a dairy herd using a control population.

A selection experiment was conducted to assess the change in production resulting from selection with artificial insemination using a randomly maintained control. The experiment continued for 16 yr with approximately 20 lactations in each of the two breeding groups annually. Selection and control production was compared using 1) intraseason herdmate comparisons (weighted) and 2) mixed model maximum likelihood estimates of year-season effects (adjusted). The regressions of differences in milk yield on years for the two approaches were 110 kg for milk (weighted) and 108 kg for milk (adjusted), corrected for inbreeding. The regressions for fat were 3.9 kg for fat (weighted) and 4.2 kg for fat (adjusted). These regression coefficients were 1.6 and 1.5% of the least squares means for milk (6987 kg) and fat (259 kg), respectively. Definitive trends were not evident for differences between the two groups in percent fat, percent SNF, days open, final type classification score, or heart girth. Even with the limited numbers in the closed control population, inbreeding, and nonrandomness in culling of females or in the choice of dams of bulls appeared to have little impact on control mean during the 16 yr.

Animals↗

PubMed1987

[Effect of growth hormone on meat production in heifers].

Short-term treatment of bovines with growth hormone (GH) has shown that GH increases nitrogen balance. In the present experiment we studied the effects of exogenous GH on weight gain, feed efficiency, carcass grade and meat quality. Twenty heifers of the Belgian White Blue breed were used. After an 8-week pre-experimental period, during which we recorded individual growth rates, the animals were allotted to two groups for 18 weeks; one group (T) received daily 50 micrograms of pituitary GH per kg of liveweight, whereas the other group (C) was given a placebo. Long-term treatment with GH induced weight gain (C: 117.1 +/- 25.5 kg; T: 144.6 +/- 23.7 kg; 23.5% increase, P less than 0.05) and increased feed efficiency (C: 10.34 +/- 2.04 kg DM/kg weight gain; T: 8.24 +/- 1.03 kg DM/kg weight gain). Digestibility of diet dry matter was identical in both groups (C: 60.1 +/- 2.7%; T: 59.8 +/- 2.4%). There were no difference in the classification of group C and T carcasses according to the EUROP pattern and after monocostal segment dissection (7th rib). Furthermore, the meat analysis of the two groups was identical with respect to protein, fat and collagen content. This was also true for tenderness, pH and juice loss after cooking. We concluded that stimulation of bovine weight gain by GH was significant and efficient while meat quality and carcass grade were not improved.

Animal Husbandry↗

PubMed1978

Monitoring of plasma and milk progesterone for evaluation of postpartum estrous cycles and early pregnancy in mares.

Plasma and milk progesterone concentrations in 13 mares were determined 3 times a week for 5 months, beginning at parturition. The estrous cycle was divided into 2 phases. Estrus was considered to occur when the plasma progesterone concentration was less than 1 ng/ml, with diestrus occurring when plasma progesterone content was greater than or equal to 1 ng/ml. Based on this classification, the period of estrus averaged 8.9 days, diestrus averaged 13.9 days, and the estrous cycle averaged 22.8 days. During estrus, the progesterone concentration in plasma averaged 0.4 ng/ml and in milk averaged 2.2 ng/ml. During diestrus corresponding values were 6.8 and 6.9 ng/ml. In the early pregnant mare, the average progesterone concentration was 5.4 ng/ml for plasma and 4.7 ng/ml for milk. The progesterone pattern was similar in milk and in blood plasma, and the correlation between 362 paired values was 0.7 (P less than 0.01). It was concluded that the estrous cycle of the mare can be mapped accurately by measuring plasma or milk progesterone content at 2- to 3-day intervals. A breeding program based on monitoring blood progesterone was outlined.

Animals↗

PubMed1986

The clinical significance of blast cell morphology in childhood lymphoblastic leukaemia.

The morphological classification of ALL based on the FAB co-operative group's criteria is capable of identifying 10-15% of children with L2 disease who, given similar treatment, will fare less well than the 85-90% with the L1 variant. The significant features defining L2 morphology are a low cellular nuclear: cytoplasmic ratio and the presence of nucleoli. Children with L2 disease do not have higher leucocyte counts but are older, have "common" ALL less frequently, and more often have well-preserved marrow function at diagnosis. Their poor outlook is manifest not only by their higher relapse rate but also by a higher proportion failing to remit in the first instance. L2 morphology does not necessarily "breed true" and can arise in a small proportion of patients with previous L1 disease at the time of relapse. Other striking morphological features of lymphoblasts, including azurophil granules, vacuoles and "hand mirror" cells, have yet to have their significance defined, though the latter feature may be an unfavourable finding.

Child↗

PubMed1979

A review of third phalanx fractures in the horse: sixty-five cases.

Sixty-five cases of third phalanx (P3) fracture were retrieved from 20,638 case records at Michigan State University's Veterinary Clinical Center between Feb 1, 1964 and July 1, 1977. The fractures were classified by anatomic location, using a numbering system. Data from case records indicated the most common P3 fracture involved the articular surface of the coffin joint (53 of 65 cases; 81.5%). The greatest occurrence of P3 fracture was in Standardbreds (31 of 65 cases). Thirty-three of the 65 fractures were in geldings of all breeds; 57 of the cases were distributed among racing horses of four breeds; and the mean ages at time of fracture for these groups were 4.9 to 5.3 years. The most common cause of P3 fracture was racing injury. Of 57 P3 fractures of the forelimb, 51 (89.5%) involved the lateral aspect of the left limb or medial aspect of the right limb--the part of each foot supporting most of the horse's weight in turns, while racing counterclockwise. Methods and response of treatment were evaluated by questionnaires obtained from 31 of the 65 owners. This questionnaire solicited information from owners and trainers as to: (1) cause, (2) treatment, (3) outcome, and (4) the use of the horse. Results were tabulated for: (1) questionnaire information obtained, (2) a classification system for P3 fractures, based on the anatomic location of the fracture, and (3) distribution of fractures between limbs and position (medial vs lateral) within limbs.

Animals↗

PubMed2026

Genome-wide variation analysis of two Salvia hispanica L. genotypes and implication for associations with metabolic and adaptive traits.

BACKGROUND: Advances in next-generation sequencing have accelerated genome-wide exploration of genetic diversity in underutilized oilseed crops. Salvia hispanica L. (chia), a high-nutrient pseudocereal rich in omega-3 fatty acids, is increasingly valued for its health benefits and commercial potential, yet it remains poorly characterized at the genomic level. Understanding the scale and nature of genomic variation is essential for improving complex traits such as oil yield, stress tolerance, and seed quality. METHODS: Two contrasting chia genotypes, Black-chia (CACH-B) and White- chia (CACH-W), were resequenced using the Bio-Resequencing Toolkit (BRT) pipeline. High-coverage sequencing, with a mapping rate exceeding 99% and an average depth of approximately 28×, facilitated the detection and annotation of single-nucleotide polymorphisms (SNPs), insertions and deletions (InDels), copy-number variations (CNVs), and structural variants (SVs). The functional classification of variant impacts enabled the identification of genes potentially linked to metabolic and adaptive traits. RESULTS: A total of 1.97 million SNPs, 401,493 InDels, 836 CNVs, and 15,288 SVs were identified across the chia genome. Notably, approximately 53% of exonic SNPs were non-synonymous (dN/dS ≈ 1.28), predominantly affecting lipid metabolism, transcriptional regulation, and stress response pathways, potentially altering key agronomic traits. In addition, CNV hotspots were concentrated in chromosomes 3 and 6, overlapping MYB, WRKY, and bZIP transcription factor loci, may potentially be involved in stress tolerance and yield. Furthermore, structural rearrangements, including inversions and duplications within the FAD2, FAD3, and CYP450 gene clusters, were potentially associated with seed pigmentation and omega-3 biosynthesis, pointing to their potential breeding relevance. Observed heterozygosity (Hₒ ≈ 0.71) and nucleotide diversity (π ≈ 7 × 10-3) indicated moderate to high allelic richness. In addition, the low FST value (0.038) indicates substantial genomic similarity between the two genotypes. CONCLUSION: This study presents the first comprehensive map integrating SNPs, CNVs, and SVs in S. hispanica L. The results reveal a structurally dynamic genome characterized by substantial sequence and structural variation, providing valuable insights into genomic diversity and potential adaptive mechanisms in chia. The coexistence of high SNP diversity and abundant structural variation underpins chia's nutritional specialization and environmental resilience. These results deliver a foundational genomic resource for marker-assisted breeding, genome-wide association studies, and the development of climate-resilient chia cultivars.

Copy-number variation, structural variation↗

PubMed1987

Mouse thiopurine methyltransferase pharmacogenetics: monogenic inheritance.

Thiopurine methyltransferase (TPMT) catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds such as the drug 6-mercaptopurine. In humans, TPMT activity is inherited as a monogenic trait. It would be useful if there were an animal model in which the genetic regulation of TPMT could be studied. Average TPMT activities in livers of C57BL/6J (B6) and AKR/J (AK) mice were only 17 to 29% of average activities in livers of like-sexed DBA/2J (D2) mice. Average TPMT activities in kidneys of B6 and AK mice were only 41 to 45% of average activities in kidneys of like-sexed D2 mice. Breeding experiments were performed to study the possible role of inheritance in regulating variations in TPMT activity in these mice. TPMT activities in livers and kidneys of F1 (hybrid) animals (N = 38) from D2 X B6 matings were intermediate to those in the parental strains but were closer to D2 than to B6 values, an observation that suggested partial dominance of the D2 phenotype. The results of studies of F2 (N = 107) and backcross (N = 102) animals derived from these matings were compatible with autosomal recessive inheritance of the trait of low TPMT activity in these mouse strains. Of the F2 animals, 27.1% were included in a "low" TPMT subgroup when enzyme activities in livers and kidneys were both used for phenotypic classification. TPMT activities in livers and kidneys of F1 mice (N = 40) from D2 X AK matings were also intermediate to those in the parental strains but were closer to D2 than to AK values.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1977

Breeding of a rabbit strain of hyperlipidemia and characteristic of this strain (author's transl).

We attempted the breeding experiment in order to establish an inbred strain of disease model animal derived from a spontaneously hyperlipidemic rabbit (HLR). The clinical, pathological features and breeding results of HLR strain were as follows; 1) A prominent clinical feature of this strain was conspicuous elevation of serous lipid ingredients including cholesterol (S-ch), beta-lipoprotein (beta-LP). The S-ch level was 400 +/- 70 mg/dl, the beta-LP level 9.1 +/- 1.5 unit and the total lipid 1146 +/- 270 mg/dl in the HLR strain. These values were approximately 9, 10 and 4 times higher than those of normal rabbits respectively. 2) After sacrifice, aterosclerotic lesions were evident extensively in the aorta and renal arteries. Nodular of plane xanthomas were conspicuous over the articuli digitorum of manus and pedis. 3) HLR strain indicated a striking resemblance of the condition to type III hyperlipidemia in the Frederickson's classification of hyperlipidemia in man. 4) Inbreeding was carried out in two lines, viz. sister-brother mating, parent-offspring mating and back crossing. As the result, the second filial generation showed an incidence of HLR of 15.0%, the third generation 50.0%, and the fourth generation 56.1% respectively. In the fourth generation, the coefficients of inbreeding and relationship were 50% and 72.3% respectively.

Animals↗

PubMed2002

Fertility of broiler breeders following categorization by the OptiBreed sperm quality index when hens are inseminated with a constant number of sperm.

If semen quality was known prior to insemination, sperm doses could possibly be decreased, maximizing the number of hens inseminated. The sperm quality index (SQI), an indicator of overall semen quality, is determined by the number of deflections in a light path due to sperm movement inside a capillary tube. The objectives of this study were 1) to determine the age at which the SQI becomes a static predictor of semen quality and 2) to determine if fertility of males with a higher SQI responds more favorably to insemination dose reduction than that of males with a lower SQI. Weekly from 23 to 32 wk of age, 144 Cobb males were tested for SQI. At 32 wk of age, males were placed into four groups that represented the SQI population quartiles as follows: poor, fair, good, and best. A fifth SQI group, uncategorized, was created to determine fertility of the original population by mixing equal amounts of semen from each of the four groups. Semen was collected weekly from 33 to 40 wk of age from 18 males in each of the four groups, pooled by group, and used to inseminate 30 hens per group with 50 or 100 million sperm. Eggs were collected daily, incubated, and broken out to determine fertility. Correlation coefficients between weekly SQI results and overall averages for individual males indicated that the SQI stabilized after the birds were 28 wk of age. The main effect for SQI selection revealed that the best SQI group had the highest fertility (88%), which did not differ from the good (83%) or fair group (82%) but was greater than the uncategorized group (80%). Fertilities of the top three groups and the uncategorized group were higher than the poor group (63%) (P < 0.0001, SEM 2.18). In addition, there was an interaction between SQI classification and insemination dose. Fertilities of the top three SQI groups were similar at the 50 and 100 million sperm doses. However, the poor and uncategorized SQI groups had lower fertility at the 50 million dose as compared to the 100 million dose. By categorizing males into SQI groups after 28 wk of age, insemination dose can be reduced, maximizing a male's fertilizing potential.

Animals↗

PubMed1982

Prognosis as the critical variable in classification of the functional psychoses.

Kraepelin used a disease entity concept to define the two major functional psychoses (i.e., distinctive patterns of onset, symptoms, and course of illness). There have been many subsequent studies using patient outcome or distribution of illness among relatives to test the validity of these nosological classes. Differences between chronic schizophrenia and (predominantly nonchronic) affective disorders are often reported, but it is difficult to discern whether these differences are associated with diagnostic symptomatology rather than premorbid prognostic status. The evidence suggesting a genetic link between good prognosis schizophrenia and affective disorders may be misinterpreted if premorbid prognostic processes are the distinguishing heritable components between chronic schizophrenia and nonchronic illnesses. Which components of severe psychiatric disorders are heritable is not yet clear. The developmental, psychological and neurological processes associated with premorbid and early morbid characteristics of illness appear good candidates. Considering such variables as prognostic (early morbid or premorbid) rather than diagnostic would permit examination of class assignment (e.g., schizophrenia, bipolar affective disorder) and premorbid development. The literature now suggests that chronicity in psychiatric illness breeds true and that symptom constellations breed true. Both of these views may be corrects, and the taxing study design required to simultaneously study these two attributes of disease entities is warranted. This may establish good prognosis schizophrenia as a "third psychosis," or may affirm its standing within traditional nosology.

Bipolar Disorder↗

PubMed2004

Evaluation of environmental risk factors for leptospirosis in dogs: 36 cases (1997-2002).

OBJECTIVE: To identify environmental risk factors for leptospirosis. DESIGN: Retrospective study. ANIMALS: 36 dogs with leptospirosis and 138 dogs seronegative for leptospirosis as determined by microscopic agglutination test for antibodies against Leptospira spp. PROCEDURES: Medical records of dogs evaluated for leptospirosis from 1997 though 2002 were identified. Owner address was used to geocode locations of dogs, and location-specific environmental risk factor data were obtained by use of a geographic information system. Risk of leptospirosis was estimated by odds ratios, controlling for potential confounding by dog age, sex, and breed. RESULTS: Leptospirosis in 19 of the 30 dogs in which an infecting Leptospira serovar could be identified was associated with Leptospira kirschneri serovar grippotyphosa infection. Dogs in which a diagnosis of leptospirosis was made, and dogs with leptospirosis caused by L kirschneri serovar grippotyphosa, were more likely to have addresses located in areas classified as rural in 1990 but urban in 2000. By use of information on recent urbanization and a logistic regression model, the status of 81.6% and 89.8% of dogs with leptospirosis and leptospirosis caused by serovar grippotyphosa, respectively, were correctly classified. Other environmental variables (proximity to streams, recreational areas, farmland, wetlands, areas subject to flooding, and areas with poor drainage; annual rainfall; and county cattle or pig population) did not significantly improve accuracy of classification. CONCLUSIONS AND CLINICAL RELEVANCE: Dogs in periurban areas are at greater risk of leptospirosis. Vaccination of dogs in these areas to protect against leptospirosis should be considered.

Animals↗

PubMed2000

Identification of RAPD markers linked to A and B genome sequences in Musa L.

Plantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of diploid, triploid, and tetraploid clones with different permutations of the A and B genomes. Thus, dessert and highland bananas are classified mainly as AAA, plantains are AAB, and cooking bananas are ABB. Classification of Musa into genomic groups has been based on morphological characteristics. This study aimed to identify RAPD (random amplified polymorphic DNA) markers for the A and B genomes. Eighty 10-mer Operon primers were used to amplify DNA from M. acuminata subsp. burmannicoides clone 'Calcutta 4' (AA genomes) and M. balbisiana clone 'Honduras' (BB genomes). Three primers (A17, A18, and D10) that produced unique genome-specific fragments in the two species were identified. These primers were tested in a sample of 40 genotypes representing various genome combinations. The RAPD markers were able to elucidate the genome composition of all the genotypes. The results showed that RAPD analysis can provide a quick and reliable system for genome identification in Musa that could facilitate genome characterization and manipulations in breeding lines.

Animals↗

PubMed1996

Relationship between parental cardiac status in Cavalier King Charles spaniels and prevalence and severity of chronic valvular disease in offspring.

OBJECTIVE: To study the relationship between parental cardiac status in Cavalier King Charles Spaniels and development of chronic valvular disease (CVD) in offspring. DESIGN: Historical cohort. ANIMALS: 54 female and 53 male Cavalier King Charles Spaniel offspring. PROCEDURE: 7 sires, selected on the basis of their liability to develop CVD, were screened for clinical signs of CVD and assigned to 1 of 3 groups (late, intermediate, and early onset of CVD). The mates of these sires (30 dams) were selected and classified likewise, and 107 offspring produced in 1988 from matings between these parents were screened for clinical signs of CVD at a mean age of 5.3 +/- 0.3 years. RESULTS: 55% of the offspring were free from clinical signs of CVD, whereas 45% had cardiac murmurs of low or moderate intensity. The proportion of offspring with heart murmurs and the intensity of murmurs were significantly greater with increased parental classification. More males than females had developed murmurs, and murmurs of moderate intensity also were more prevalent in males. Results of multiple-regression analysis indicated that mean parental classification and sex had significant effects on proportion of offspring with murmurs and their intensity. Additionally, age affected disease prevalence and severity, despite the narrow range in age of offspring examined. CLINICAL IMPLICATIONS: Parental CVD status is an important factor influencing the probability of heart murmurs and their intensity in offspring. The results of this study indicate that CVD development is a polygenic threshold trait and that sex of the offspring influences threshold levels.

Age Factors↗