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At least 271 records · Page 15Linked to original sources
PubMed2005

Recommended standards for biochemical markers of myocardial damage: All Wales Clinical Biochemistry Audit Group.

The effective use of cardiac-specific troponin estimations in the diagnosis of acute myocardial infarction (AMI) is clouded by the imprecise definition surrounding the decision limits. This has led to a wide variation of criteria for the diagnosis of myocardial infarction. A survey of troponin measurements in Welsh laboratories, undertaken in 2003 under the auspices of the All Wales Clinical Biochemistry Audit Group, revealed significant variations in laboratory and clinical practice. Extensive discussion and consultation led by a working group of clinical biochemists and cardiologists in Wales culminated in recommendations concerning the use of troponin assays to establish myocardial damage. The key recommendations are: Cardiac troponin (T or I) should be the first-line test for myocardial damage; Two samples should be collected, at admission and 12-24 h later. The first sample is used for 'rule in' purposes, but not to 'rule out' myocardial damage; Only one threshold (cut-off) value for troponin should be quoted on laboratory reports, values above which are indicative of myocardial damage. A study by the Wales External Quality Assurance Scheme (WEQAS) enabled the derivation of the recommended cut-off concentrations of troponin for defining myocardial damage, defined for each assay as the concentration that can be reliably distinguished, with a confidence interval of 99%, from the 99th percentile reference limit. These recommended standards provide a rationale for a uniform approach for troponin assays for patients with chest pain, working towards a standardized approach to the diagnosis and management of patients presenting with acute coronary syndromes.

Biomarkers↗
PubMed2003

National academy of clinical biochemistry laboratory medicine practice guidelines: recommendations for the use of laboratory tests to support poisoned patients who present to the emergency department.

BACKGROUND: Exposure to drugs and toxins is a major cause for patients' visits to the emergency department (ED). METHODS: Recommendations for the use of clinical laboratory tests were prepared by an expert panel of analytical toxicologists and ED physicians specializing in clinical toxicology. These recommendations were posted on the world wide web and presented in open forum at several clinical chemistry and clinical toxicology meetings. RESULTS: A menu of important stat serum and urine toxicology tests was prepared for clinical laboratories who provide clinical toxicology services. For drugs-of-abuse intoxication, most ED physicians do not rely on results of urine drug testing for emergent management decisions. This is in part because immunoassays, although rapid, have limitations in sensitivity and specificity and chromatographic assays, which are more definitive, are more labor-intensive. Ethyl alcohol is widely tested in the ED, and breath testing is a convenient procedure. Determinations made within the ED, however, require oversight by the clinical laboratory. Testing for toxic alcohols is needed, but rapid commercial assays are not available. The laboratory must provide stat assays for acetaminophen, salicylates, co-oximetry, cholinesterase, iron, and some therapeutic drugs, such as lithium and digoxin. Exposure to other heavy metals requires laboratory support for specimen collection but not for emergent testing. CONCLUSIONS: Improvements are needed for immunoassays, particularly for amphetamines, benzodiazepines, opioids, and tricyclic antidepressants. Assays for new drugs of abuse must also be developed to meet changing abuse patterns. As no clinical laboratory can provide services to meet all needs, the National Academy of Clinical Biochemistry Committee recommends establishment of regional centers for specialized toxicology testing.

Antidepressive Agents, Tricyclic↗
PubMed2004

Recent developments in the biochemistry of chromium(III).

Chromium is generally believed to be an essential trace element and to have a role in maintaining proper carbohydrate and lipid metabolism, probably by enhancing insulin signaling. Three recent events have strongly influenced biochemical and nutritional studies of Cr(III): (1) the Food and Nutrition Board's new daily adequate intake (AI) of Cr, (2) the Food Standards Agency's determination that Cr picolinate might have the potential to cause cancer, and (3) the National Institutes of Health's program announcement "Chromium as an adjuvant therapy for type 2 diabetes and impaired glucose tolerance." A discussion of these three events allows the current understanding of the nutritional biochemistry of Cr to be outlined.

Animals↗
PubMedDate not supplied

[Foundation and development of biochemistry at the Imperial Kharkov University].

The article summarizes the biochemical researches carried out at Kharkiv Imperial University from the middle of XIX century up to the cessation of its existence in 1920 as a result of transformation into the Kharkiv Institute of National Education. Scientific activity at the Chair of Medical Chemistry at Medical Department is described in details. Information on professors who led the chair and their researches are represented. Among them a great attention is spared to the Kharkiv works of such famous scientists as A. Danilevsky and V. Gulevich, who made a great contribution to the development of Russian and world biochemistry. There are also some resordes about researches of biological and physiological chemistry carried out at other chairs of Medical Department and Department of Physics and Mathematics of the Kharkiv University. In particular, the article presents the works of well-known plant physiologists and biochemists prof. V. Palladin and V. Zalessky, and the endocrinological researches led by prof. A. Reprev.

Biochemistry↗
PubMed1999

[Hommage to M.L. Girard: gestation and birth of biochemistry in hospitals].

Prof Claude Dreux pays hommage to Prof Maurice-Louis Girard and relates the gestation and the birth of biochemistry in hospitals. He successively evokes Hippocrates, Galen, Paracelsius, Robert Boyle, Nicolas Vauquelin, J.J. Von Scherer, J.F. Zeller, G.H. Esbach, Apollinaire Bouchardat, Georges Deniges, Paul Yvon, Ambard, Loiseleur, Leon Grimbert, Paul Fleury, Jean-Emile Courtois and last but not the least Maurice-Louis Girard.

Biochemistry↗
PubMedDate not supplied

[Department of Biochemistry of Microorganisms--start of the path (1951-1973)].

Creation of the Department of Biochemistry of Microorganisms at the Institute of Microbiology and Virology of the Academy of Sciences of Ukrainian SSR in the 30's of the last century was determined by a necessity of profound investigation of vital activity biochemism of microorganisms from various systematic groups which were studied in microbiological department of the Institute. Such complexity can explain certain diversity of the Department research at initial stages of its existence. The research of saccharose transformation into dextran Leuconostoc mesenteroides, when production solutions become slingy at sugar-refinaries, was one of the first most significant works of the Department. The enzyme saccharose-glycosyl-transferase performing this process was described for the first time. A cycle of works on the study of enzymes splitting lactose in milk under the effect of Streptococcus lactis has been carried out. Complex investigation of a number of proteins, polysaccharides, enzymes in enterobacteria has shown that the blocking of the enzyme aldolase is one of the reasons of alkali formation. A method has been developed for isolation of arenarin, antibiotic of plant origin, from sandy everlasting, the nature of its acting basis has been established. Nufarin, an active antibiotic, was isolated from the roots of white water lily when studying nitrogen fixation processes, special attention was given to interaction of hydrogenase and enzymes, taking part in nitrogen fixation, to the effect of ATP on these processes, ways of its synthesis, localization of ATPase in the cell membranes. Works on the study of lypopolysaccharides and polysaccharides of Gram-negative enterobacteria, bacteria of Pseudomonas genus were started with the purpose to use the obtained data to specify systematic propositions of the investigated microorganisms. Further on these works became the basis of thematic department. There are numerous reviews dedicated to their development.

Academies and Institutes↗
PubMed2003

[50 years of the Institute for Research in Pharmacy and Biochemistry].

After the nationalization of pharmaceutical industry and establishment of the United Pharmaceutical Enterprises in 1946, the Research and Control Institute (VKU) was established in Prague in 1947 to support the development of research, manufacture, and control of drugs. After other measures of nationalization and unification of research, in 1951 the research sections of the VKU and the pharmaceutical sections of the Research Institute of CCHZ were fused to form the Research Institute for Pharmacy and Biochemistry (VUFB). The section the VUFB taking care of the quality of production was transformed into the Pharmaceutical Control Institute (KUF). Each of the institutes had its own farm for breeding experimental animals outside Prague. In 1958, KUF was transformed into the Research Institute of Medicinal Plants (VULERO) and later, in 1960, into the Research Institute of Natural Substances (VUPL), which eventually fused with VUFB in 1967. During the years of increasing activities of VUFB, a chemical pilot plant was established in Olomouc, and a department of clinical pharmacology in Plzen. Research activities of VUFB were aimed to search for original drugs and to develop non-proprietary medicines in the field of the central and vegetative nervous systems, blood circulation, inflammatory processes, microbial infections, carcinostatic drugs, etc. In natural substances, the greatest attention was paid to ergot alkaloids, but the extent of research also included the constituents of the poppy, periwinkle, and other plants. An important part of the programme was also the breeding and cultivation of selected medicinal plants. The results of research were continuously published in both inland and foreign scholarly journals and at conferences and congresses. Every year, usually more than 100 papers were published and 40-50 for patents applications were submitted. The activity of the Institute resulted in the production of 30 original substances which were introduced into therapeutic practice. Twenty of them are still commonly prescribed. Some of these original substances got into foreign markets, particularly Prothiaden and Trimepranol represented important exports. In addition, 83 non-proprietary drugs were introduced into the inland market.

Academies and Institutes↗
PubMed2002

Thermodynamics, biochemistry and microscopy for the 21st century.

The aim of biology is the study of the properties of cells in living intact animals. Many steps of research procedures in biochemistry change the entropy, and therefore, the free energy of the chemical reactions from those in vivo. Sections prepared for histology, histochemistry, electron microscopy and immunocytochemistry, yield limited information about the original cells in life, although their problems have been documented. Approaches to the biological and histological difficulties are suggested.

Artifacts↗
PubMedDate not supplied

[Some results and prospects of studies in the area of marine biochemistry and biotechnology].

Recent studies on bioactive metabolites from marine macro- and microorganisms are reviewed with 83 refs. Structures of new sulphated and glycosylated secondary metabolites, which have been reported to have antifungal, immunomodulatory, and cytotoxic properties, are given. Some peculiarities of biosynthesis of natural compounds in marine organisms are revealed. It was shown that some natural products, isolated earlier from sponges, are produced by microbial symbionts. Different physiological activities associated with 8000 marine microbial (mainly symbiotic) strains are discussed as well as some prospects of marine biochemistry and biotechnology development.

Animals↗
PubMedDate not supplied

Small group discussion followed by presentation as a revision exercise at the end of a teaching module in biochemistry.

BACKGROUND: We evaluated the effect of student-dominated small group discussion followed by faculty-moderated presentation as a revision exercise after completion of a teaching module in biochemistry. We assessed the understanding of graduate medical students on the topic and the gain in retention of information, if any, after 15 days. METHODS: Small group discussions involving 11-12 students in each group were conducted on 12 application-oriented problems in 'amino acid metabolism'. A group leader among the students helped to conduct the discussions. While two-thirds of the problems were taken up after the discussion during faculty- or student-moderated interactive presentations, the remaining were not. The effects on low-, medium- and high achievers were evaluated by a pre-test and post-test with multiple choice questions immediately after the session. A subjective feedback was also obtained. To test short-term memory, a post-test with the same multiple choice questions was conducted after 15 days. RESULTS: The exercise was effective and equally beneficial for low-, medium- and high achievers. The gain was maximum when faculty moderated the presentation session. The students' retention of their gain after 15 days was complete. All the students wanted more such sessions in future. CONCLUSION: Student-dominated small group discussion followed by a faculty-moderated presentation is an effective, revision exercise for undergraduate medical students.

Attitude of Health Personnel↗
PubMed2003

A sixty-year evolution of biochemistry at McGill University.

The Department of Biochemistry at McGill University was inaugurated close to a century after the Medical School was founded. The roots of the Department, however, can be found at the very beginning of the Medical School in 1829. Because several of the founding faculty members of the Medical School were educated in Edinburgh, McGill's early medical program bore the imprint of the Edinburgh school--particularly in the importance placed on instruction in chemistry and on basic research. This survey of the development of a university department is structured on the succession of department chairs, and describes how the Department's scientific, pedagogical, and administrative activities were influenced by the particular abilities and dispositions of the individuals who were at the helm. It explains how the growth of external research institutes influenced the Department's evolution, and cites some of the noteworthy contributions of its members.

Biochemistry↗
PubMed2006

[Trends in the clinical biochemistry and laboratory diagnostics].

Clinical biochemistry and laboratory diagnostics develops dynamically namely due to the advance of modern analytical techniques, information technologies and with new findings in the molecular biology. According to data from the USA, 70 to 80% of medical decisions in the hospitals depend on comparatively not expensive laboratory tests which represent only 3 to 5% of the health care costs. There are three essential approach models for the automation and consolidation of laboratories in the world. Japan, USA and countries of EU stand for prototype of those directions. Japan has a high level of total laboratory automation starting with the preanalytical phase, over the analytical phase to the postanalytical phase with the partial consolidation of the discipline. In the US the high degree of laboratory consolidation is preferred with so-called modular automation. EU countries prefer high degree of modular automation, including the preanalytical processing of samples. International trends in the laboratory diagnostics manifest in the Czech Republic by modular systems in several laboratories. They can increase the effectiveness of laboratories and subsequently erase differences between the routine and statim examination and develop of molecular-biological techniques. Development of laboratory diagnostic technologies and their economical accessibility are the compulsory condition of acceleration of the diagnostics and treatment, leading to high quality, focussed and finally effectivuess of health care.

Biochemistry↗
PubMedDate not supplied

[Differential forms of self-directed study of biochemistry in medical schools].

Methods of self-directed study of biochemistry by students are considered in connection with initial levels of their theoretical knowledge and laboratory experience. This type of training involved individual tasks and free discussion on the results obtained. Optimal results were observed in organization of self-directed work in small groups.

Biochemistry↗
PubMed1989

Clinical biochemistry in Singapore.

During the period from 1982 to mid-1989, there has been very significant changes and improvement in the practice and application of clinical biochemistry in Singapore. This paper highlights recent developments in the repertoire of biochemical analyses, utilisation of laboratory tests, biochemical screening in preventive health care, handling and delivery of patient specimens, advances in analytical techniques, automation, computerisation, quality assurance, training of laboratory personnel, and professional activities. It also draws attention to the restructuring of the Singapore General Hospital and the government Department of Pathology which frees these government institutions from the constraints of the civil service so that they would be sufficiently flexible to respond readily to the changing needs and expectations of the patients and users of laboratory services. This is the most prominent event and policy change in the history of health care services in Singapore that is expected to result in more cost-effective utilisation of resources and greater improvement in the quality of health care.

Automation↗
PubMed1985

[Principles of cooperation between the specialties of internal medicine, pathology and clinical biochemistry].

The optimal utilization of the knowledge and possibilities of pathological and clinical biochemistry presumes a close cooperation between it and the clinical specialties. The common working team of the GDR Society of Internal Medicine and the GDR Society for Clinical Chemistry and Laboratory Diagnostics makes theses of the central points of the cooperation in care, education, further education and postgraduate study and in research a subject for discussion. As essential tasks in the process of medical care are regarded the balance of the examination programme standing at the disposal, the establishment of diagnostic programmes, the establishment of organisational measures, the ascertainment of a use according to indication, the guarantee of the representance of examination material, the control of plausibility and the interpretation of test results. Since the realization of the tasks to a large extent depends on the cooperation of the specialities in education, further education and postgraduate study during the further education the clinician should become acquainted with the possibilities, the limits and the prerequisites for the performance of laboratory diagnostic investigations, the clinical biochemist with the problems of medical care and the value of the laboratory diagnosis in the total process of the treatment. In the field of research the result is a necessary cooperation in the clarification of patho-biochemical mechanisms, in the search for suitable laboratory diagnostic parameters for diagnostics and control of the course as well as in the statement of the validity of laboratory diagnostic parameters and parameter combinations taking into consideration the factors expenses, benefit and risk as well as further diagnostic possibilities.

Biochemistry↗
PubMedDate not supplied

[Contribution of R. V. Chagovets, member of the Ukrainian SSR Academy of Sciences, to the development of biochemistry].

The paper is concerned with the main stages of scientific activity of R.V. Chagovets (1904-1982). Member Academy of Sciences of the Ukrainian SSR in the field of biochemistry of muscles, metabolism and biochemical functions of vitamins and their derivatives. The creative heredity of the scientist served as a theoretical ground of the present-day vitaminology, base for development of the nutrition theory, practical recommendations and suggestions in public health and husbandry opened the pathways for experimental solution of the problems concerning vitamin and coenzyme metabolism regulation, elucidation of molecular mechanisms of vitamin and coenzyme functioning in the cell.

Biochemistry↗
PubMedDate not supplied

[Various methodological aspects of biochemistry].

Certain methodological problems of present-day biochemistry are considered. Comprehension of the mechanisms of biochemical processes is shown to be possible on the basis of the structural and functional analysis at the molecular level. Ways of development and use of structural and functional approach to studies of supermolecular structures, cells and organisms on the whole are discussed.

Biochemical Phenomena↗