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Relationships between behavioral and meat quality characteristics of pigs raised under barren and enriched housing conditions.

In this study the effects of barren vs enriched housing conditions of pigs on their behavior during the lairage period (2-h holding period before slaughter), carcass characteristics, postmortem muscle metabolism, and meat quality were studied. The barren housing system was defined by common intensive housing conditions (i.e., with slatted floors and recommended space allowances), whereas the enriched environment incorporated extra space and straw for manipulation. Salivary cortisol concentrations were measured before transport and at the end of the lairage period. During the lairage period the percentage of time spent walking and fighting by the pigs was registered. Carcass characteristics such as weight, meat percentage, and backfat thickness were determined. At 5 min, 45 min, 4 h, and 24 h postmortem, pH, temperature, and lactate concentrations were determined in the longissimus lumborum (LL) and biceps femoris (BF) muscles. Capillarization of the muscle, mean muscle fiber area, and color and drip loss after 2 and 5 d of storage were determined for both muscle types. Pigs from the barren environment had a significantly higher increase in cortisol from farm to slaughter, but no differences in behavior were observed during the lairage period. Carcass characteristics did not differ between pigs from barren and those from enriched housing conditions. Postmortem lactate formation was significantly lower in LL muscles of enriched pigs at 4 and 24 h postmortem. Capillary density and mean muscle fiber area did not differ between the groups of pigs. The percentage of drip loss at 2 and 5 d after storage of LL muscle samples from enriched-housed pigs was significantly lower than that of the barren-housed pigs. Similar tendencies were found for the BF muscle from pigs kept in an enriched environment, but these were not statistically significant. The housing system did not affect meat color. It is concluded that on-farm improvement of animal welfare by environmental enrichment can also lead to beneficial economic effects after slaughter by improving the water-holding capacity of pork.

Abattoirs↗

Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs.

A study of the effects of conjugated linoleic acid (CLA) on the belly firmness and fatty acid composition of genetically lean pigs was conducted. From 75 to 120 kg live weight, 30 gilts were allowed ad libitum access to a corn-soybean meal diet supplemented with either 1% CLA oil (CLA-60) or 1% sunflower oil (SFO) or were fed the sunflower oil-supplemented diet restricted to the amount consumed by pigs fed the CLA-60 diet (RSFO). Conjugated linoleic acid oil consists of 60% positional and geometric isomers of CLA. Pigs fed SFO exhibited higher average daily gains (0.98 vs 0.80 kg/d, P < 0.01) than RSFO-fed pigs, but there were no effects of dietary treatment on feed intake or feed efficiency. Dietary treatment did not affect (P > 0.05) backfat thickness or longissimus muscle area. Bellies of gilts fed CLA-60 were subjectively evaluated to be firmer (2.91 vs 2.43 or 2.07 +/- 0.13, P < 0.01) than those of SFO- or RSFO-fed gilts, respectively. The longissimus muscle of gilts fed CLA-60 contained more saturated fatty acids (39.77 vs. 36.04 or 36.73 +/- 0.74%, P < 0.001) and less unsaturated fatty acids (60.23 vs 63.96 or 63.27 +/- 0.74%, P < 0.001) than that of gilts fed SFO or RSFO, respectively. The belly fat of gilts fed CLA-60 contained more saturated fatty acids (44.45 vs. 37.50 or 36.60 +/- 0.46%, P < 0.001) and less unsaturated fatty acids (54.78 vs. 61.75 or 62.47 +/- 0.46%, P < 0.001), resulting in lower iodine values (57.69 vs 66.37 or 65.62 +/- 0.91, P < 0.001) than that of gilts fed SFO or RSFO, respectively. Gilts fed CLA-60 accumulated more CLA in the longissimus muscle (0.55 vs 0.09 or 0.09 +/- 0.03%, P < 0.01) and belly fat (1.56 vs. 0.13 or 0.13 +/- 0.15%, P < 0.001) than did gilts fed SFO or RSFO, respectively. Dietary treatment did not affect (P > 0.05) 24-h pH, drip loss or subjective quality evaluations of the longissimus muscle. The effect of supplemental CLA to improve belly firmness is of practical significance and may provide a nutritional solution to carcass fat and belly firmness problems, thereby enhancing the overall value of extremely lean carcasses.

Adipose Tissue↗

Breed effects on growth performance, carcass characteristics, fatty acid composition, and palatability attributes in finishing steers.

Crossbred steers (n = 136) were used to assess breed differences in growth performance, carcass characteristics, fatty acid composition (total lipids and phospholipids), and palatability attributes of longissimus muscle. A multiple regression model was applied to crossbreeding data to estimate genetic differences between Simmental and Red Angus at the same level of backfat finish (10 mm). Simmental spent 71 more (P < 0.001) days on feed to acquire the same degree of backfat thickness as Red Angus, had heavier (P < 0.001) slaughter weights, larger (P = 0.002) longissimus muscle area, and increased (P = 0.023) lean yield. Average daily gain did not differ (P = 0.297) between breeds. Simmental were less (P = 0.012) efficient in converting feed to gain than Red Angus. Generally, there were few breed differences in palatability attributes for longissimus and semitendinosus muscles, with the exception of increased (P < 0.05) beef flavor scores for Simmental beef vs Red Angus beef across both muscles. For total lipids, concentrations of myristoleic acid (14:1), palmitoleic acid (16:1), and vaccenic acid (18:1n-7), along with n-6 to n-3 fatty acid (n-6:n-3) ratio, were greater (P < 0.05) in Simmental than Red Angus. In contrast, concentrations of margaric acid (17:0), eicosapentaenoic acid (20:5n-3), and total n-3 polyunsaturated fatty acids (n-3 PUFA) were greater (P < 0.05) in Red Angus than Simmental. For phospholipids, Simmental had lower (P < 0.05) amounts of 20:5n-3, docosahexaenoic acid (22:6n-3), and n-3 PUFA, with a greater (P = 0.017) n-6:n-3 ratio. Activity of delta9-desaturase enzyme in the conversion of palmitic acid (16:0) to 16:1 was greater (P = 0.001) in total lipids from Simmental as compared with Red Angus. A genetic basis for fatty acid differences is suggested, although the biological and practical significance needs to be demonstrated.

Animals↗

Antioxidant status affects color stability and tenderness of calcium chloride-injected beef.

The objectives of this study were to determine whether vitamin E supplementation influences color and tenderness of beef injected with calcium chloride. Market heifers (n = 12) were fed a standard finishing diet with minimal levels of vitamin E (NE group). Another 12 market heifers were fed the NE diet with the inclusion of 1,000 IU/d of DL-alpha-tocopherol per animal for the last 125 d on feed (E group). Animals were slaughtered after 125 d on the diets and upon reaching an ultrasound backfat thickness > 10 mm. Half of the longissimus muscles from each treatment group (NE and E) were pumped to 10% over the original weight with 250 mM CaCl2 (Ca) at 24 h postmortem. Remaining muscles (NE and E) were pumped to 10% over the original weight with water (NC) at 24 h postmortem. After equilibrating overnight, steaks (2.54 cm) were overwrapped with O2-permeable film and stored for 7 d after injection. Hunter "L," "a," and "b" values were obtained each day of storage. Trained panelists evaluated color on d 1, 4, and 7 after injection. 2-Thiobarbituric acid-reactive substances (TBARS) values were measured on d 1 and 7 after injection. Warner-Bratzler (W-B) shear force values and trained sensory panel evaluations at 1, 3, and 7 d after injection were obtained. Immunoblotting techniques were used to monitor the 30-kDa degradation product of troponin-T at 1, 3, and 7 d after injection. At 4 d after injection, E/Ca steaks were the least discolored (P < 0.05). The E/Ca steak TBARS values were not significantly different from values for NE/NC steaks at 7 d after injection, whereas NE/Ca steaks had greater (P < 0.05) TBARS values after 7 d following injection compared with all other groups. Treatment with Ca resulted in higher off-flavor scores (P < 0.05). The E/Ca samples had the most rapid tenderization and proteolysis of all treatment groups. Warner-Bratzler shear values were lower in the E/Ca samples than in the E/NC samples at 1, 3, and 7 d after injection (P < 0.05). No difference in shear force was noted between NE/Ca and NE/NC samples at any time point. No difference in sensory tenderness was noted between NE/Ca and NE/NC samples at 1 d after injection. However, Ca-injected samples (NE/Ca and E/Ca) were rated as being significantly more tender than their uninjected counterparts (NE/NC and E/NC) at 3 and 7 d after injection. Injection of CaCl2 may result in more rapid and immediate tenderization if beef from animals supplemented with vitamin E is used. Vitamin E incorporation into muscle tissue may potentiate the action of exogenously added calcium by protecting the calpains from oxidation.

Animals↗

Effect of group size on pig performance in a wean-to-finish production system.

Crossbred pigs (n = 1,400) were used to evaluate the effect of group size (25 vs 50 vs 100 pigs/pen) in a wean-to-finish production system on growth performance and carcass measures. Pigs were weaned at 17 d (range = 15 to 19) of age with a mean initial BW of 5.9 +/- 0.02 kg and taken to a final mean pen weight of 116 +/- 0.9 kg. A 10-phase dietary regimen was used, and pigs had free access to feed and water. Feeder-trough space (4.3 cm/pig) and floor-area allowance (0.68 m2/pig) were the same for all group sizes. Compared to groups of 25, pigs in groups of 50 and 100 animals were lighter (P < 0.001) at the end of wk 8 after weaning and had lower (3%, P < 0.01) ADG and gain:feed (G/F) but similar (P > 0.05) ADFI during the first 8 wk of the study. At the end of the study, pig weight and the coefficient of variation in pig weight within a pen were similar (P > 0.05) across group sizes. During the period from 8 wk after weaning to the end of the study, pigs in groups of 100 compared to 50 animals had greater (3%, P < 0.01) ADG, and pigs in groups of 25 were intermediate for ADG. Average daily feed intake during this period was similar (P > 0.05) for all group sizes; however, G/F was greater (3%, P < 0.01) for groups of 100 compared to 25 or 50 animals. For the overall study period, ADG, ADFI, and G/F from weaning to slaughter weight were similar across group sizes (P > 0.05; 655, 648, and 658 g; 1,759, 1,755, and 1,759 g; and 0.37, 0.37, and 0.37; for ADG, ADFI, and G/F, respectively, for groups of 25, 50, and 100 pigs, respectively). Mortality was similar (P > 0.05) across group sizes; however, morbidity (pigs removed due to poor health or injury) was higher in groups of 25 pigs compared to the other two group sizes (7.0, 3.5, and 3.9% for groups of 25, 50, and 100, respectively; P < 0.05). Group-size treatment did not affect (P > 0.05) carcass dressing percentage, backfat thickness, or loin-eye depth. In summary, growth performance from weaning to market weight was not affected by group size.

Animal Husbandry↗

Genotype x environment interactions for postweaning performance in crossbred calves grazing winter wheat pasture or dormant native prairie.

Data from 403 calves from Angus, Brahman, and reciprocal-cross cows sired by Polled Hereford bulls were used to evaluate the impact of postweaning backgrounding forages on postweaning BW, gains, and carcass traits. Calves were born (spring of 1991 through 1994) and reared on either endophyte-infected tall fescue or common bermudagrass pastures. After weaning, calves were transported 360 km to the Grazinglands Research Laboratory, west of El Reno, OK, and, within breed and preweaning forage, were assigned to one of the following winter stocker treatments: 1) winter wheat pasture or 2) dormant native prairie plus supplemental CP. In March, winter stocker treatments were ended and calves were grazed as a single group on cool-season grasses until early July (1992, 1993, and 1994) or late May (1995), when the feedlot phase began. In the feedlot, calves were fed a high concentrate diet for an average of 120 d until a backfat thickness of > 10 mm was reached. Calves were shipped in truck load lots to Amarillo, TX (350 km), for processing and collection of carcass data. Averaged over calf breed group, calves wintered on wheat pasture gained faster (P < 0.01) during the stocker phase (0.71 vs 0.43 kg); had heavier (P < 0.01) final feedlot weights (535 vs 512 kg); lower feedlot (P < 0.01) ADG (1.37 vs 1.53 kg); heavier (P < 0.01) carcass weights (337 vs 315 kg); larger (P < 0.01) longissimus muscle (84.9 vs 81.8 cm2); higher percentage (P < 0.01) of kidney, heart, and pelvic fat (2.32 vs 2.26); and higher (P < 0.01) dressing percentage (62.2 vs 61.3) than calves backgrounded on native prairie. Maternal heterosis for stocker ADG was evident in calves backgrounded on native prairie but not on winter wheat (P < 0.10), but the two environments were similar in maternal heterosis for feedlot ADG and carcass traits. Calves wintered on native prairie were restricted in growth and expressed compensatory gain during the feedlot phase but not during the spring stocker phase. Dormant native grasses can be used to winter stocker calves excess to the winter wheat pasture needs, but ownership of these calves would have to be retained through the feedlot phase to realize any advantage of built-in compensatory gain. Finally, these data suggest that expression of maternal heterosis for weight gain is more likely in calves backgrounded on native prairie than in calves grazed on winter wheat.

Animal Feed↗

Effect of different protein sources on growth and carcass traits in growing-finishing pigs.

Crossbred gilts (n = 180) and barrows (n = 180) from the Louisiana State University (LSU) Agricultural Center and the University of Illinois (UI) were used to compare the effect of soybean meal in swine diets, relative to other protein sources, on growth performance and carcass traits of growing-finishing pigs. Four replications with five pigs each at each location were allotted to nine dietary treatments: soybean meal control (SBM), crystalline AA (corn-AA), extruded soybeans (ESB), canola meal (CAN), peanut meal (PNT), sunflower meal (SFLR), ground peas, meat and bone meal (MBM), and poultry by-product meal (PLTY). The diets were formulated to meet or exceed NRC nutrient requirements and to have equal Lys:ME according to dietary phase and sex. Corn was the grain source in all diets and the protein sources were the sole source of supplemental protein in all diets except when AA were added to meet the requirement. Pigs (three per pen at each location) were killed at an average final BW of 114 kg in the LSU or UI Meat Science Laboratories. Pigs fed SBM had greater (P < 0.05) ADG than pigs fed the corn-AA, CAN, SFLR, MBM, or PLTY and greater (P < 0.05) ADFI relative to pigs fed the corn-AA, ESB, MBM, or PLTY. Gain:feed was decreased (P < 0.05) in pigs fed corn-AA or SFLR but increased (P < 0.05) in pigs fed ESB compared with pigs fed the SBM diet. Loin muscle area was decreased (P < 0.05) in pigs fed the corn-AA or MBM diets compared with pigs fed the SBM diet. Tenth-rib backfat thickness was greater (P < 0.10) in pigs fed corn-AA, peas, or MBM than in those fed SBM. The NPPC percentage acceptable quality lean and kilograms of lean were decreased (P < 0.10) in pigs fed corn-AA, peas, or MBM compared with those fed SBM. Results from this experiment suggest that pigs fed SBM have equal or better growth performance and carcass traits than pigs fed other protein sources.

Animal Feed↗

Impact of spontaneous exercise on performance, meat quality, and muscle fiber characteristics of growing/finishing pigs.

The objective of this experiment was to determine if increased space and exercise for finisher pigs (0.90 vs 9.45 m2/pig) affects performance, meat quality, or muscle fiber characteristics. Newsham barrows (n = 32, 4 pens/treatment) were placed in one of two space allocations: control space allowance (CONT) or in a long pen with increased space allowance (10x). Pigs were weighed every 28 d and feed intake/pen was calculated. Pigs were filmed for behavioral analysis on d 70 and 100 using video recorders to determine walking distances over a 24-h period. After a 5-h transport and 2-h rest period, pigs (approximately 115 kg) were slaughtered on the same day at a commercial facility. Muscle samples were obtained from the longissimus lumborum (LL) and semimembranosus (SM) muscles within 1 h postmortem for muscle fiber typing. Backfat thickness and pH decline were measured on the left side of each carcass. After 24-h chilling, a boneless loin was collected from each pig and stored at 2 degrees C until analyzed. On d 14 postmortem, loins were cut at the 10th rib for color evaluations, and chops were cut for Warner-Bratzler shear (WBS) force and sensory analysis. Histochemical staining methods were used for the detection of type I, IIA, and IIB/X muscle fiber types. There were no significant differences (P > 0.10) in live weight, ADG, ADFI, or G:F ratio of the two experimental groups evaluated. Pigs finished in 10x pens walked a greater (P < 0.01) distance over a 24-h period than pigs finished in the CONT pens. Pigs finished in the lOx pens were fatter (P < 0.05) at the last lumbar vertebra than pigs finished in the CONT pens, but no significant differences were found in loineye area, loin color, marbling scores, WBS, sensory panel scores, retail display measures, or muscle fiber type percentages. Expanded space allowance to increase exercise resulted in no improvements in pig performance, pork loin measures, or muscle characteristics.

Animal Husbandry↗

Performance of male pigs immunized against GnRH is related to the time of onset of biological response.

In this study, the performance of male pigs immunized against GnRH was determined in relation to the onset of their biological response to the immunization. Pigs were immunized at 9 and 17 wk of age and were housed in a pen together with both a surgically castrated and an intact boar littermate. Feed intake was restricted to 2.8 to 3.2 times maintenance requirement for energy. Animals were weighed weekly and slaughtered at 108 kg BW. Depending on the time of onset of the response after immunization in terms of biological effects, immunized pigs were retrospectively grouped into two categories. One category consisted of the immunized pigs, which had undetectable or low levels of LH and testosterone at the time of booster immunization-known as "early" responding immunocastrates (E-IM, n = 8), whereas the "late" responding immunocastrates (L-IM, n = 7) had substantial LH and testosterone levels at that time. This dichotomy of the response to immunization also was reflected in testis weight, with 17 g and 40 g for E-IM and L-IM pigs, respectively. At slaughter, testis size and weight were reduced (P < 0.001) in the immunocastrated pigs as compared to the intact boars. Androstenone concentrations in backfat of all immunocastrated pigs were undetectable. Growth performance (i.e., ADG and feed efficiency [FE, g gain/kg feed]), was better in boars and L-IM pigs than in surgical castrates and E-IM pigs (P < 0.05). Average daily gain and FE did not differ between E-IM pigs and the surgical castrates, but intact boars performed better than L-IM (P < 0.02). There were no significant differences in carcass quality (backfat thickness and meat percentage) between boars and surgical castrates at slaughter. However, for both characteristics L-IM pigs and intact boars performed better (P < 0.03) than E-IM pigs. Thus, growth performance in L-IM is better than in either E-IM or surgical castrates.

Androstenes↗

Genetic parameter estimates of yearling live animal ultrasonic measurements in Brangus cattle.

The objective of this study was to estimate genetic parameters for real-time ultrasound measurements of longissimus muscle area (LMA), 12th rib backfat thickness (FT), percent intramuscular fat (IMF), and yearling weight (YW) for 1,299 yearling Brangus bulls and heifers. A single ultrasound technician performed all measurements. The number of observations was 1,298, 1,298, 1,215, and 1,170 for LMA, FT, IMF, and YW, respectively. Genetic parameters were estimated for each trait using single- and multiple-trait derivative-free restricted maximal likelihood. Fixed effects were contemporary group (defined as same sex, same age within six months, and same environment), and days of age as a covariate. Correlations were estimated from two-trait models. Heritabilities for LMA, FT, IMF, and YW were 0.31, 0.26, 0.16, and 0.53, respectively. Genetic correlations between LMA and FT, LMA and IMF, LMA and YW, FT and IMF, FT and YW, and IMF and YW were 0.09, 0.25, 0.44, 0.36, 0.42, and 0.31, respectively. Yearling live animal ultrasonic measurements can be used as a selection tool in breeding cattle for the improvement of carcass traits.

Adipose Tissue↗

Effects of supplemental vitamin E on performance, health, and humoral immune response of beef cattle.

Three experiments were conducted to examine the effect of dietary vitamin E on receiving performance and health and on finishing performance of beef cattle. One hundred twenty beef steers (Exp. 1; initial BW = 173 kg) and 200 beef heifers (Exp. 2; initial BW = 204 kg) were assigned randomly to one of three treatment diets formulated to supply 285, 570, or 1,140 IU/animal daily of supplemental vitamin E during the receiving period. Average daily gain, gain:feed, and DMI were calculated every 14 d, with pen as the experimental unit. Morbidity and retreatment data were analyzed using a nonparametric procedure. After the receiving period, cattle were assigned to a grazing period followed by a finishing program and fed until slaughter. In Exp. 3, 17 beef steers were used to evaluate effects of the same three vitamin E levels on humoral immune response to an ovalbumin vaccine given on d 0 and 14. Jugular blood samples were collected on d 0, 7, 14, and 21. In Exp. 1, vitamin E did not affect (P > 0.10) ADG, DMI, or gain:feed for d 0 to 14, 14 to 28, or 0 to 28. No effects were noted for percentage of morbidity; however, cattle receiving 1,140 IU/d had a numerically (P = 0.15) lower incidence of retreatment. During the 91-d finishing phase, a quadratic effect (P < 0.08) was noted for DMI, ADG, backfat thickness, longissimus muscle area, and yield grade. In Exp. 2, a tendency for a linear (P = 0.10) increase in ADG was observed for the first 14 d of receiving; however, ADG decreased linearly (P = 0.06) with vitamin E concentration thereafter. For the 28-d period, ADG and DMI did not differ among treatments, but gain:feed decreased linearly (P < 0.05) for d 14 to 28 and for d 0 to 28. No effects on percentage morbidity were noted in Exp. 2, and no differences were detected for ADG, gain:feed, or DMI for the 98-d finishing period. There was a linear increase in yield grade (P < 0.05) and a linear (P < 0.08) decrease in longissimus muscle area with increasing vitamin E. Heifers receiving 570 IU of vitamin E during the receiving period tended to have a higher (P < 0.09) dressing percentage at slaughter. In Exp. 3, no significant differences were detected in serum IgG titers to ovalbumin on d 0, 7 or 14; however, on d 21, a linear increase (P = 0.07) in serum IgG titers was noted with supplemental vitamin E. Supplemental vitamin E had limited effects on performance; however, effects on humoral immune response and recovery from respiratory disease warrant further research.

Age Factors↗

The impact of longissimus glycolytic potential and short-term feeding of magnesium sulfate heptahydrate prior to slaughter on carcass characteristics and pork quality.

The objective of this study was to compare the effects of longissimus glycolytic potential (GP) and of time of feeding of supplemental magnesium sulfate heptahydrate on carcass and pork quality traits. The study was carried out in a 2 x 2 x 4 factorial arrangement; the treatments were sex (castrate vs gilt), GP (Low [normal] vs High), and time of feeding of magnesium sulfate-fortified diets (0 [control] vs 2 vs 3 vs 5 d prior to slaughter). Glycolytic potential was determined on a biopsy sample of longissimus from the live animal prior to the start of the study. A total of 144 pigs were allotted to the feeding-time treatments on the basis of sex (castrate and gilt), weight, and GP. Pigs were placed in individual pens and had free access to water. Prior to the start of the study, pigs were given ad libitum access to a standard finisher diet. During the study, animals were fed at a fixed level of 2.75 kg of a standard finisher diet/day; the fortified diet contained 3.2 g/d of additional magnesium. At the end of the feeding period, animals were transported to a commercial packing facility and slaughtered within 15 min of arrival. Fresh meat quality was measured on the longissimus. There were no treatment interactions. Carcass traits were similar across time of feeding treatments. Backfat thickness at the last lumbar vertebra and 10th rib were lower (P < 0 .05) for High than for Low GP pigs. High GP pigs had lower ultimate pH (P < 0.001) and higher drip (P < 0.05) and purge loss (P < 0.01) than Low GP pigs. Drip loss was reduced (P < 0.05) for pigs fed the magnesium-fortified diet for 5 and 2 but not for 3 d compared to controls (8.98, 7.29, 7.89, and 7.41 for the 0-, 2-, 3-, and 5-d treatments, respectively, SEM 0.447). Purge loss was similar for all of the time of feeding treatments. Longissimus L* values were lower (P < 0.05) for the 2-d treatment than for the controls. Results from this study suggest an inconsistent effect of short-term feeding of magnesium sulfate on muscle color and drip loss in pigs with both Low (normal) and High GP.

Adipose Tissue↗

Diverse birth and rearing environment effects on pig growth and meat quality.

Birth and rearing conditions were evaluated for their effects on pig growth, body composition, and pork quality using 48 barrows during the spring and summer months. Pigs were either farrowed in indoor crates or outdoor huts. At weaning, indoor-born and outdoor-born pigs were randomly allotted to indoor or outdoor treatments for growing/finishing. Body weight data were collected. Pigs were transported 5 h to a commercial processing plant, allowed 2 h of rest, and then processed as a group under commercial conditions. Boneless loins were collected from the left side of each carcass and aged for 14 d. Objective and subjective color measurements were taken on the longissimus muscle at the 10th rib on d 14 postmortem. Loin chops were evaluated for sensory attributes, shear force, and retail display features. Pigs born outdoors were heavier and had greater ADG at all growth intervals after weaning (d 28, 56, 112, and final weight, P < 0. 05) than pigs born indoors. Outdoor-born pigs had heavier carcass weights (91.2 vs 81.3+/-3.4 kg, P < 0.001), larger loineye areas (54.6 vs 49.7+/-0.2 cm , P < 0.05), and higher pork flavor intensity scores (6.5 vs 6.1+/-0.10, P < 0.01) than indoor-born pigs. Birth x rearing environment interactions were not significant for most measures. Backfat measurements at the last rib were greater (3.2 vs 2.8+/-0.05 cm, P < 0.05) for the pigs reared outdoors than for the pigs reared indoors. Pigs finished outdoors had more reddish pink color scores, lower shear force values, and lower L* values, indicating darker-colored pork, compared with pigs finished indoors (P < 0.05). Pig birth environment played a significant role in improving growth rates of outdoor-born pigs and increasing pork flavor intensity scores of loin chops from pigs born outdoors. Finishing pigs outdoors may improve pork color and tenderness but also may increase backfat thickness when they are fed conventional diets.

Animal Husbandry↗

Supplemental betaine and peroxide-treated feather meal for finishing cattle.

These studies evaluated the effects of betaine, provided either as feed-grade betaine or as concentrated separator by-product (CSB; desugared beet molasses), on performance and carcass characteristics of finishing cattle. In Exp. 1, 175 steers (410 kg initial BW) were fed a finishing diet based on steam-flaked and dry-rolled corn, and treatments included 10.5 and 21 g/d feed-grade betaine and 250 and 500 g/d CSB (supplying 15.5 and 31 g/d of betaine, respectively). Steers fed feed-grade betaine had greater (linear and quadratic effects, P < 0.1) DMI than control steers, but ADG and gain efficiencies were not affected by treatment. Dressing percent and backfat thickness was greater (P < 0.1) for steers that received feed-grade betaine than for controls. Longissimus muscle area was lower (P < 0.1) for steers supplemented with either feed-grade betaine or CSB than for control steers. Yield grades were higher for cattle receiving feed-grade betaine (quadratic effect, P < 0.1) than for control steers. Marbling scores were not affected by supplemental betaine, but the percentage of carcasses grading USDA Select was lower (linear and quadratic effects, P < 0.1) for steers fed feed-grade betaine than for control steers, predominantly due to a greater percentage grading USDA Choice. In Exp. 2, 312 heifers (343 kg initial BW) were used in a finishing study to evaluate the effects of graded levels of feed-grade betaine and peroxide-treated feather meal on performance and carcass characteristics. Treatments included two finishing diets (containing peroxide-treated or untreated feather meal) and four levels (0, 4, 8, and 12 g/d) of feed-grade betaine arranged in a 2 x 4 factorial. No significant interactions occurred between treatment of feather meal and betaine. Treatment of feather meal with hydrogen peroxide (5% wt/wt) increased in situ protein degradability but did not alter DMI, ADG, gain efficiencies, or carcass characteristics of heifers when it replaced untreated feather meal in the diet. Top-dressing feed-grade betaine to the diets had no effect on DMI, ADG, and gain efficiencies. Marbling scores were greater (cubic effect, P < 0.05) for heifers fed diets top-dressed with 4 and 12 g/d of feed-grade betaine, but other carcass characteristics were not altered significantly. Overall, feed-grade betaine and CSB did not alter growth performance, but did have minor effects on carcass characteristics.

Adipose Tissue↗

Effect of carbohydrate source on growth performance, carcass traits, and meat quality of growing-finishing pigs.

Two experiments were conducted to determine the effect of substituting a more available dietary carbohydrate (CHO) for portions of corn or fat in the diet on growth performance, carcass traits, meat quality, and serum or plasma metabolites in growing-finishing pigs. A three-phase feeding program was used with corn-soybean meal diets formulated to provide 105% of the Lys requirement for barrows or gilts gaining 325 g of lean daily in Exp. 1 or gilts gaining 350 g of lean daily in Exp. 2. Diets were isoenergetic within experiments. All other nutrients met or exceeded suggested requirements. In Exp. 1, pigs were allotted to three dietary treatments (0, 7.5, or 15.0% sucrose), with three replications of barrows and three replications of gilts, and with three or four pigs per replicate pen; average initial and final BW were 25.2 and 106.7 kg. In Exp. 2, gilts were allotted to two dietary treatments (waxy [high amylopectin] or nonwaxy [75% amylopectin and 25% amylose] corn as the grain source), with five replications of four gilts per replicate pen; average initial and final BW were 37.7 and 100.0 kg. In Exp. 1, ADG and gain:feed ratio increased linearly (P < 0.02) as dietary sucrose increased. Minolta color scores, a* and b*, and drip loss (P < 0.06) also increased linearly with added sucrose. In Exp. 2, ADG, carcass weight and length, and the Minolta a* value were greater for pigs fed waxy corn (P < 0.08) than for those fed nonwaxy corn. Feed intake, longissimus muscle area, 10th-rib and average backfat thickness, dressing percentage, fat-free lean, percentage of lean and muscling, lean gain per day, total fat, percentage fat, lean:fat ratio, serum or plasma metabolites (Exp. 1: serum urea N; Exp. 2: serum urea N, and plasma nonesterified fatty acids, triacylglycerols, total and high-density lipoprotein cholesterol, insulin, and total protein), pH of the longissimus muscle, and subjective muscle scores (color, firmness-wetness, and marbling) were not affected by diet in either experiment. In summary, increasing availability of dietary CHO in growing-finishing pig diets improved growth performance, but it did not affect carcass traits.

Adipose Tissue↗

Influence of oscillating dietary crude protein concentration on performance, acid-base balance, and nitrogen excretion of steers.

Decreasing dietary N inputs into beef cattle feeding operations could potentially decrease environmental concerns relating to air and water quality. Previous studies with sheep suggest that oscillating dietary CP concentrations may improve N use efficiency and thereby decrease dietary N requirements. Therefore, two studies were conducted to determine the effects of oscillating dietary CP concentrations on performance, acid-base balance, and manure characteristics of steers fed high-concentrate diets. Steers were fed to a constant backfat thickness in both studies. In the first trial, 92 steers (mean BW = 408 +/- 2.8 kg; four pens/treatment) were fed the following diets: 1) constant 12% CP, 2) constant 14% CP, and 3) 10 and 14% CP oscillated at 2-d intervals. Steer performance and carcass characteristics were measured. In the second trial, 27 steers were individually fed the same three experimental dietary regimens (nine steers/treatment). Animal performance, arterial acid-base balance, plasma metabolites, and fecal characteristics were measured. In both trials, steers fed the 14% CP diet tended (P < 0.10) to have greater ADG and gain:feed than steers fed the 12% CP diet. Steers fed the oscillating CP regimen had intermediate performance. In Trial 1, steers fed the 14% CP diet tended (P = 0.09) to have smaller longissimus area and higher quality grades than steers fed the oscillating CP regimen. Protein retentions (g/d) calculated from NRC (2000) equations were greater (P = 0.04) for steers fed the 14% CP diet than steers fed the 12% CP diet. Steers fed the oscillating CP regimen tended (P = 0.08) to have greater calculated protein retention (g/d) than steers fed the 12% CP diet. Steers fed the 14% CP diet had greater (P < 0.05) calculated urinary N excretion than steers fed the 12% CP or oscillating CP regimens. Venous plasma concentrations of urea N were greater (P < 0.001) in steers fed the 14% CP diet than in steers fed the 12% CP diet; steers fed the oscillating CP regimen were intermediate but fluctuated over days. Based on arterial blood gas concentrations, acid-base balance was not significantly affected by dietary CP regimen. Results of these trials suggest that the CP requirement of steers in these studies was greater than 12% of the diet DM, and/or that the degradable CP requirement was greater than 6.3% of diet DM. However, the effects of oscillating dietary CP were minimal.

Acid-Base Equilibrium↗

Quantitative trait loci analysis for growth and carcass traits in a Meishan x Duroc F2 resource population.

We constructed a pig F2 resource population by crossing a Meishan sow and a Duroc boar to locate economically important trait loci. The F2 generation was composed of 865 animals (450 males and 415 females) from four F1 males and 24 F1 females and was genotyped for 180 informative microsatellite markers spanning 2,263.6 cM of the whole pig genome. Results of the genome scan showed evidence for significant quantitative trait loci (<1% genomewise error rate) affecting weight at 30 d and average daily gain on Sus scrofa chromosome (SSC) 6, carcass yield on SSC 7, backfat thickness on SSC 7 and SSC X, vertebra number on SSC 1 and SSC 7, loin muscle area on SSC 1 and SSC 7, moisture on SSC 13, intramuscular fat content on SSC 7, and testicular weight on SSC 3 and SSC X. Moreover, 5% genomewise significant QTL were found for birth weight on SSC 7, average daily gain on SSC 4, carcass length on SSC 6, SSC 7, and SSC X and lightness (L value) on SSC 3. We identified 38 QTL for 28 traits at the 5% genomewise level. Of the 38 QTL, 24 QTL for 17 traits were significant at the 1% genomewise level. Analysis of marker genotypes supported the breed of origin results and provided further evidence that a suggestive QTL for circumference of cannon bone also was segregating within the Meishan parent. We identified genomic regions related with growth and meat quality traits. Fine mapping will be required for their application in introgression programs and gene cloning.

Adipose Tissue↗

Growth performance, dry matter and nitrogen digestibilities, serum profile, and carcass and meat quality of pigs with distinct genotypes.

We investigated the effect of distinct genotypes on growth performance, DM and N digestibilities, serum metabolite and hormonal profiles, and carcass and meat quality of pigs. Eight control-line and eight select-line pigs with an equal number of gilts and castrated males per genotype were chosen from the group of pigs subjected to selection for lean growth efficiency. Pigs were housed individually and allowed ad libitum access to common grower, finisher 1, and finisher 2 diets when they reached approximately 20, 50, and 80 kg, respectively, and water throughout the study. Although genotype had no effect on growth performance during the finisher 2 phase and overall, select-line pigs grew faster and more efficiently (P < 0.05) during the grower and finisher 1 phases than did control-line pigs. Dry matter and N digestibilities during the grower phase were lower (P < 0.05) in select-line pigs compared with control-line pigs. Select-line pigs had less ultrasound backfat (P < 0.05) at the end of the grower and finisher 2 phases. Serum urea N (P < 0.05) and leptin concentrations were lower in select-line pigs than in control-line pigs, but the effect of genotype on serum glucose, triglyceride, or insulin concentration was rather inconsistent. Select-line pigs had heavier heart (P < 0.05), liver (P = 0.08), and kidneys (P < 0.01), implying a higher metabolic activity. Less 10th-rib carcass backfat (P < 0.01) and a trend for larger carcass longissimus muscle area (P = 0.10) were reflected in the greater (P < 0.01) rate and efficiency of lean accretion in select-line pigs. Select-line pigs had lower subjective meat color (P < 0.01), marbling (P < 0.05), and firmness (P < 0.01) scores. Final serum leptin concentration was correlated positively with carcass backfat thickness (r = 0.73; P < 0.01) and negatively with overall feed intake (r = -0.77; P < 0.01). These results indicate that pigs with distinct genotypes exhibited differences in the growth rate, metabolite and hormonal profiles, and body composition. Further research is necessary to determine whether pigs with distinct genotypes respond differently to dietary manipulations, which would have an effect on developing optimal feeding strategies for efficient and sustainable pig production.

Adipose Tissue↗