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At least 271 records · Page 15Linked to original sources
PubMed1980

Bacteriophage N3 of Haemophilus influenzae. III. Characterization of bacteriophage N3 DNA.

DNA extracted from phage N3 grown in Haemophilus influenzae strain JC9 was examined by velocity and equilibrium centrifugation, electron microscopy and thermal melting curves. This double stranded DNA, molecular weight 26X10(6) daltons, has continuous single strands at 13X10(6) daltons as shown by alkaline sucrose gradient centrifugation. The duplex form readily aggregates in neutral salt solutions at even moderate ionic strength. Aggregation may be due to N3 DNA having "sticky" ends. CsCl equilibrium centrifugation measurements and thermal melting curves indicate a G+C content of 43 mol %.

Bacteriophages↗
PubMed1984

[Effect of the polymerase activity of DNA polymerase I on the development of the temperate bacteriophages Mu, lambda red- and lambda red-gam-. I. The effect of the polymerase activity of DNA polymerase I on the development of bacteriophages lambda red- and lambda red-gam-].

Phages lambda c1857 red3 and lambda bio10 in Escherichia coli K-12 cells with impaired function of DNA-polymerase I polymerizing activity are shown to restore their normal development level when exonucleases V and I are removed from cells. In other words, an indirect involvement of DNA-polymerase I in the development of phages lambda defective in general recombination systems and gene gam functions has been established. A probability of DNA-polymerase I participation in the recombination stage of phage lambda development in E. coli K-12 cells is discussed.

Adsorption↗
PubMed1978

Explanations accounting for transduction by bacteriophage lambda in maltose negative bacteriophage lambda resistant mutants of Escherichia coli K-12.

Entry of DNA from lambda phages particles into lambdarMAl- mutants of Escherichia coli K-12 is shown to be due to two distinguishable processes. One, residual transduction, results from a low level expression of lamB. The other one, background transduction, is independent of gene lamB. Interpretations are presented for these results. It is propos that residual transduction is due to a weak promoter pB3 located within or near the distal part of the gene preceeding lamB in the same operon. It is proposed that background transduction is due to a secondary receptor structure for phage lambda. Finally a tentative hypothesis relatin pB3 to insertion sequences is presented.

Coliphages↗