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At least 271 records · Page 15Linked to original sources
PubMed2001

Evaluation of phytotoxicity and genotoxicity of uranyl nitrate in Allium assay system.

Uranyl nitrate inhibited root growth of Allium cepa at > or = 25 microM concentration. Fluorimetric analysis of metal uptake indicated the entry and accumulation of uranium into the root cell. Uranyl nitrate was neither clastogenic nor aneugenic as it failed to induce micronuclei significantly, but between 25 and 100 microM concentration, it increased significantly the frequency of sister chromatid exchange over that of control, implying its genotoxicity that possibly interfered with DNA replication and/or repair process.

Allium↗
PubMed2000

[A new flavonoid glycoside from Allium cepa L. var agrogatum Don].

AIM: To investigate the chemical constituents from the bulb stems of the Chinese medicinal plant Allium cepa L. var. agrogatum Don. METHODS: The crude extracts of the bulb stem were purified by Sephadex LH-20 chromatography. IR, UV, MS, 1H, 13CNMR, 1H-1H COSY, 1H-13C COSY and HMBC spectra were used to determine the structure of the isolated constituents. RESULTS: A new compound named quercetin 3'-methoxy-4'-O-beta-D-glucopyranoside(I), together with three known compounds kaempferol(II), quercetin 4'-O-beta-D-glucopyranoside(III) and quercetin 3,4'-di-O-beta-D-glucopyranoside(IV) were isolated. CONCLUSION: Quercetin 3'-methoxy-4'-O-beta-D-glucopyranoside(I) is a new compound, compounds II-IV were isolated from this plant for the first time.

Allium↗
PubMed2001

Evaluation of impact of sewage irrigation on cytotoxicological potentialities of Chenopodium album in Allium assay.

Chenopodium album is a weed commonly consumed by North Indian population as vegetable. Plants are known to accumulate toxins from their environment. Presently, the leaf homogenates of these plants growing in a tubewell irrigated field and a sewage irrigated field were evaluated for cytotoxicological effects in Allium root tip assay. Studies revealed that Chenopodium album was mildly mitodepressive in nature and was capable of inducing chromosomal aberrations. The leaf homogenate of the plants growing in sewage irrigated fields induced more quantum of aberrations than the plants from the control site. This has a direct bearing on consumability of sewage grown vegetables and fodder.

Agriculture↗
PubMed1998

[Structural elucidation of the anticoagulation and anticancer constituents from Allium chinense].

Six compounds were isolated from the anticoagulation and anticancer fractions of the bulbs of Allium chinense G. Don. On the basis of chemical evidence and spectral analysis (IR, EI-MS, 1HNMR, 13CNMR, 1H-1H COSY, HMBC, HMQC and NOESY), their structures were established as (25R, S)-5 alpha-spirostane-3 beta-ol 3-O-(beta-D-glucopyranosyl-(1-->2)-[beta-D-glucopyranosyl-(1-->3)]-beta-D- glucopyranosyl-(1-->4)-beta-D-galactopyranoside) (1), (25R, S)-5 alpha-spirostane-3 beta-ol 3-O-(beta-D-glucopyranosyl (1-->2)-[beta-D-glucopyranosyl-(1-->3)](6-acetyl-beta-D-glucopyranosyl)- (1-->4)-beta-D-galactopyranoside) (2), (25R, S)-5 alpha-spirostane-2 alpha, 3 beta-diol 3-O-(beta-D-glucopyranosyl-(1-->2)-O-beta-D-glucopyranosyl-(1-->4)-beta-D- galactopyranoside) (3), (25S)-24-O-beta-D-glucopyranosyl-3 beta, 24 beta-dihydroxy-5 alpha-spirost-3-O-alpha-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside (4), chinenoside II (5) and 2,3,4,9-tetrahydro-1-methyl-1H-pyrido [3,4-b] indole-3-carboxylic acid (6). 4 is a new steroidal saponin, named chinenoside VI. Compounds 1 to 3 are three pairs of steroidal saponin epimers. Among them, the 25S epimer of 2 is first reported, the 25R epimer of 2 and compound 6 were isolated from this title plant for the first time. The relative configuration of compound 6 was firstly determined by NOESY spectrum, and signals of C, H were assigned definitely.

Allium↗
PubMed2002

[Disruption of organization of mitotic microtubules in root meristem cells of Allium cepa induced by chloral hydrate].

Data are presented on the effect of chlorahydrate on microtubule organization in the root meristem of Allium cepa. Our studies show that an incomplete preprophase band commonly appears during G2-prophase transition, yet the major effect is the lack of perinuclear microtubules, leading to inhibition of the prophase spindle formation and transition to C-mitosis. Upon chloralhydrate treatment of metaphase cells, we found cells with chromosomes regularly aligned within the metaphase plate and differently disorganized mitotic spindles. Concurrently, C-metaphase cells with remnants of kinetochore fibers were present. In addition, normal bipolar and abnormal irregular types of chromosome segregation were detected, this representing multipolar and diffuse anaphases. The major difference between them is the presence of polar microtubules during multipolar anaphase, and their lacking during diffuse anaphase. Alternatively, microtubule clusters between segregated groups of chromosomes are typical for cells with diffuse anaphase. During bipolar anaphase, excessive aster-like microtubules emanate from the spindle poles, and in telophase accessory phragmoplasts are observed at the cell periphery. The formation of incomplete phragmoplasts was observed after normal bipolar and abnormal chromosome segregation. We conclude that chloralhydrate may affect the nuclear surface capability to initiate the growth of perinuclear microtubules, thus blocking the prophase spindle formation. It also disturbs the spatial interaction between microtubules, which is crucial for the formation and functioning of various microtubular systems (preprophase band, spindle and phragmoplast).

Allium↗
PubMed2000

[Studies on chemical constitutents in seeds of Allium tuberosum Rottl].

OBJECTIVE: To study the chemical constituents in the seed of Allium tuberosum. METHOD: Chromatography and spectral analysis were used to isolate the constituents and elucidate their structures. RESULT: Five compounds were isolated from the alcohol extracts of the seeds and identified as nicotianoside C, (22S)-cholest-5-ene-1 beta, 3 beta, 16 beta, 22-tetrol-1-O-alpha-L-rhamnopyranosyl-16-O-beta-D-glucopyranoside, daucosterol, adenosine and thymidine. CONCLUSION: All the compounds were isolated from the seed of this plant for the first time.

Adenosine↗
PubMedDate not supplied

[Cytogenetic effects of 241Am in the Allium test].

The purpose of this study was to elucidate the genome damage induced by 241Am-irradiation using different parameters of cytogenetic evaluation in Allium-test. The root tip cells test-system for the cytogenetic effects studying was used. 241AmCl3 of different concentrations was used (1.5 x 10(-9)-1.5 x 10(-7) g/l). Water solution-to-plant transfer factor for 241Am was found to be 0.18 +/- 0.04. The internal doses of 241Am accumulated during germination were 0.37-37.00 cGy. The impact of 241Am-irradiation was evaluated on the mitotic index (MI), the yield of aberrant anaphases (AA), the distribution of chromosome aberrations number in cells and the average level of lesion of aberrant cell (LAC). Probably all these parameters are differ in sensitivity to damage factor, but only some changes in MI was revealed. It is supposed, that the absence of any changes in the distribution of chromosome aberrations number in cells and the average level of LAC in 241Am-irradiated cells confirm the absence of significant 241Am-impact on chromosomes, as the alpha-irradiation should cause significant damages in chromosomes. Although solutions of 241Am were high-concentrated, the seedlings didn't accumulate high internal doses. It appears the distribution of 241Am is a significantly heterogeneous hence it is possible the absorbed doses in nuclei can't reach the level necessary for revealing of cytogenetic effects.

Allium↗
PubMed1999

[Localization with BrUTP labeling technique of RNA polymerase II transcription in meristematic cells of Allium cepa].

The technique of pulse-labeling of cells with 5-bromouridine-5'-triphosphate (BrUTP) and immunological detection of the incorporated BrUTP is a newly-developed experimental approach to localize nascent RNA polymerase II transcripts in the nucleus. This technique has been widely used since it was first reported in 1993, but most of the studies were carried out with fluorescence microscopy and animal cells, we are still limited in the knowledge about ultrastructural localization of RP II transcripts in the nucleus of eukaryotes, especially in that of plant cells. Attempts have been made in this paper to localize nascent RP II transcripts in the nucleus of meristematic cells of Allium cepa with the technique of pulse-labeling of BrUTP and immunoelectron microscopy. After labeling with BrUTP and detection with anti-BrdU antibody, a large number of gold particles representing RP II transcripts were observed in the periphery of chromatin domains, the gold particles in the periphery accounted for 92.70% of the total gold particles in the extranucleolar regions, and the gold density of the periphery was 75.42/micron 2, much higher than that of the center of the chromatin domains and the interchromatin domains which were only 5.89/micron 2 and 2.00/micron 2 respectively, indicating that RP II transcripts were being actively synthesized in the periphery of the chromatin domains. When the specimens were treated with alpha-amanitin which can specifically inhibits the activity of RP II, gold particles in the extranucleolar regions of the specimens were decreased dramatically and the gold density of the regions dropped strikingly from 44.60/micron 2 to 2.67/micron 2, confirming that the gold particles in the regions represent RP II transcripts. The distribution of nascent RP II transcripts in the nucleus of the intact plant cells is discussed.

Allium↗
PubMed1998

[Supercritical-CO2 fluid extraction of Allium stativum oils].

Effective constituents from bulb of Allium stativum were extracted by supercritical-CO2 fluid. These constituents were analyzed by GC-MS. The results showed that oils from SFE-CO2 contained 12 components, two of them were first obtained from the plant.

Allium↗
PubMed1992

Variation of RBE between p(75) + Be and d(50) + Be neutrons determined for chromosome aberrations in Allium cepa.

The RBE of p(75) + Be neutrons relative to d(50) + Be neutrons has been determined for chromosome aberrations induced in Allium cepa (onion) roots. Two biological criteria were selected: the average number of aberrations (mainly fragments) per cell in anaphase and telophase, and the percentage of aberration-free cells. The influence of sampling time (3 to 7 h incubation) between irradiation and fixation was investigated systematically. This factor did not significantly influence the results. The RBE values of p(75) + Be neutrons compared to those of d(50) + Be neutrons were 0.85 (0.79-0.91) and 0.87 (0.80-0.95) for the first and the second criteria, respectively. In previous experiments for the same beams, we found an RBE of 0.90 (0.86-0.94) for survival of V79 cells (D0 ratio), 0.96 (0.93-0.99) for the intestinal crypt cell system, and 0.83 (0.70-0.96) for Vicia faba growth delay.

Allium↗
PubMed1992

Antimicrobial activity of naphtoquinones and Allium extracts combined with antibiotics.

Combinations of antibiotics and known antimicrobial natural products, naphtoquinones and Allium extracts, were investigated. The chessboard broth method was applied to assess the activity of these combinations against aerobic and anaerobic bacteria. If no antagonism was observed, the combinations studied lead from partial to total synergism, mainly against aerobic bacteria.

Allium↗
PubMed1992

A low molecular weight peptide from Allium porum with inhibitory activity on platelet aggregation in vitro.

A peptide factor was isolated from Allium porum with strong inhibitory activity on platelet aggregation induced by collagen or ADP. The peptide was isolated by a series of chromatographic techniques and purified to homogeneity by HPLC on RP C8 column. Its molecular weight, as it was estimated by plasma desorption mass spectrometry, is 1052 Da. Amino acid analysis showed the presence of Phe, Asp, Ser, Thr, Arg and Pro.

Allium↗
PubMed2003

[Advances in the study of Allium macrostemon Bunge].

OBJECTIVE: To review the progress in the study of chemical ingrediens, pharmacological effects and clinical application of Allium macrostemon Bunge. METHOD: Documents of experimental and clinical study on A. macrostemon in recent 10 years were consulted and summarized. RESULT: A. macrostemon had many active ingredients, pharmacology effects and wild clinical application. CONCLUSION: The results provide a rational foundation for the further development and utilization of A. macrostemon.

Allium↗
PubMed2005

[Mitogenic and mutagenic effects of ionized air on Allium fistulosum L].

In the apical meristem of Allium fistulosum, the relationship between peroxide lipid oxidation, antioxidant activity, proliferative processes, the yield of chromosomal aberrations and duration the exposure to ionized air was studied. Under the influence of air oxygen ions, superoxide dismutase and catalase activities increased, proliferative processes were stimulated, and shifts occurred in the process of lipid peroxidation in cells of A. fistulosum. When these cells were treated with air oxygen for 40 min, hydrogen peroxide and iron sulfate (II) enhanced oxygen biostimulating effect via stimulation of antioxidant enzyme activity and inhibition of lipid peroxidation. Under these conditions, cell proliferation was intensified and the yield of chromosomal aberrations was reduced in A. fistulosum rootlets. When the time of seed treatment with ionized air was increased to 80 min, lipid peroxidation was activated, antioxidant enzyme activity was inhibited, and the yield of chromosomal aberration increased in seedlings. It was concluded that the biostimulating activity of ionized air was mediated by active oxygen species generated in the cell. The accumulation of TBA(thiobarbituric acid)-reactive products was shown to be related to a decrease in antioxidant enzyme activity and an increase in the yield of chromosomal aberrations. It is emphasized that the mutagenic effect of ionized air is associated with generating conditions that support Fenton reaction and OH-radical formation in the cell.

Air Ionization↗
PubMed2006

In vivo studies on the effect of Ocimum sanctum L. leaf extract in modifying the genotoxicity induced by chromium and mercury in Allium root meristems.

In vivo cytogenetic assay in Allium cepa root tip cells has been carried out to detect the modifying effect of Ocimum sanctum aqueous leaf extract against chromium (Cr) and mercury (Hg) induced genotoxicity. It was observed that the roots post-treated with the leaf extract showed highly significant (p < 0.001) recovery in mitotic index (MI) and chromosomal aberrations (CA) when compared to pre-treated (Cr/Hg) samples and the lower doses of the leaf extract were found to be more effective than higher doses. The present study reveals that the Ocimum sanctum leaf extract possesses the protective effect against Cr/Hg induced genetic damage.

Allium↗
PubMed2006

[Determination of saponin I in extracts of Allium macrostemnon].

OBJECTIVE: To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon. METHOD: The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID. RESULT: The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%). CONCLUSION: The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.

Allium↗
PubMedDate not supplied

[Dynamics of the induced chromosomal instability in welsh onion (Allium fistulosum L.): gamma irradiation of the seeds of different storage periods].

The chromosome aberrations in root meristem cells of welsh onion (Allium fistulosum L.) seeds after gamma-irradiation (5 and 10 Gy) of different-aged seeds (7, 19, 31, 43 and 55 months of storage) were studied. The irradiation dose of 5 Gy significantly increased the frequency of aberrant anaphases (FAA) for 31- and 43-months seeds; the dose of 10 Gy significantly increased the FAA in seeds of all age groups. The irradiation of young (7 months) seeds resulted in decreasing of the fraction of bridges to the control level of the old (55-months) seeds for the dose of 5 Gy and below the control level of the old seeds--for the dose of 10 Gy. Some peculiarities of cytogenetic parameters of genome instability and the germinating capacity of the seeds made it possible to suppose that the third year of storage is a critical period for the welsh onion seeds.

Allium↗
PubMed1990

Analysis of the cell cycle in the root meristem of Allium cepa under the influence of ledakrin.

The influence of a polish anticancer drug on the cell cycle using Allium test was studied. Methods of aceto-orcein squash slides, curve of labelled mitoses after 3H-thymidine incubation and cytophotometrics after Feulgen's reaction were employed. Ledakrin acts strongly antimitotically, but it does not block the cell cycle completely. The cytostatic activity of ledakrin results from its action on the interphase. The phases G1 and S are prolonged while M is unchanged after 6h incubation with ledakrin. During postincubation in water without ledakrin it was noted, at the beginning, that the mitotic activity decreases and it is brought about the lengthening of S and G2 phases. The duration of the cell cycle phases returns to the control level during further postincubation. The results of analysis of chromatin aberrations and the micronucleus test point to a mutagenic effect of ledakrin.

Allium↗