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At least 253 records · Page 14Linked to original sources
PubMed1990

[Our experience in the use of mechanical staplers in the execution of step-by-step subtotal gastrectomy].

The Authors describe their reconstruction technique after a "step" gastric resection for peptic or neoplastic pathology. The use of staplers such as TA 30 or GIA for the duodenum is reported. After skeletonizing the lesser curve up to the cardias by sectioning the left gastric artery at its origin and up to the penultimate short vessel on the greater curve, the stomach is resected distally to this two points using a TA90 staplers. So it is possible to remove the greater part of the acid-secreting area in order to avoid ulcers of the anastomosis or of the gastric stump, and also to achieve a complete lymphadenectomy in case of neoplasm. Then a Roux-en-Y gastro-jejunal anastomosis is performed using an EEA28 circular stapler. The jejunal stump is closed with a TA30. Finally, a manual jejuno-jejunal anastomosis is made at least at 50 cm from the previous one. From November 1987 to January 1989, 13 patients were operated: 9 for peptic ulcer and 4 for antrum neoplasms. There were no complications during and after the operations. Mortality was 0%. The patient, usually starts eating on the 6th or 7th day after operation, and is discharged on the 10th day. After 6 months an EGD scopy is required: the 8 performed till now have demonstrated excellent anastomosis and no peptic lesions. Scintigraphic evaluations and pH monitoring are still in course to define the gastric stump acid secretion and the absence of biliary reflux.

Anastomosis, Roux-en-Y↗
PubMed1991

First-trimester prenatal diagnosis in quintuplets: a practical approach using step-by-step embryo reduction.

A multiple pregnancy of high rank may occur in a couple at risk for a Mendelian disorder. Prenatal diagnosis is hampered by the difficulty of (1) obtaining chorionic villi from each zygote and (2) unequivocally relating each sample to the corresponding embryo. The calculation of the genetic risk according to the number of zygotes led us to propose a diagnostic strategy based on embryo reduction, a technique initially designed to improve the perinatal outcome of multiple pregnancies with normal embryos. We report a case in which this approach allowed rational use of first-trimester chorionic villus sampling in a quintuplet pregnancy at risk for non-ketotic hyperglycinaemia, resulting in the selective birth of unaffected twins.

Abortion, Therapeutic↗
PubMedDate not supplied

Low-resolution density maps from atomic models: how stepping "back" can be a step "forward".

Atomic-resolution structures have had a tremendous impact on modern biological science. Much useful information also has been gleaned by merging and correlating atomic-resolution structural details with lower-resolution (15-40 A), three-dimensional (3D) reconstructions computed from images recorded with cryo-transmission electron microscopy (cryoTEM) procedures. One way to merge these structures involves reducing the resolution of an atomic model to a level comparable to a cryoTEM reconstruction. A low-resolution density map can be derived from an atomic-resolution structure by retrieving a set of atomic coordinates editing the coordinate file, computing structure factors from the model coordinates, and computing the inverse Fourier transform of the structure factors. This method is a useful tool for structural studies primarily in combination with 3D cryoTEM reconstructions. It has been used to assess the quality of 3D reconstructions, to determine corrections for the phase-contrast transfer function of the transmission electron microscope, to calibrate the dimensions and handedness of 3D reconstructions, to produce difference maps, to model features in macromolecules or macromolecular complexes, and to generate models to initiate model-based determination of particle orientation and origin parameters for 3D reconstruction.

Algorithms↗
PubMed2025

A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes Using the Oxford Nanopore MinION.

The Oxford Nanopore (ONT) MinION enables sequencing of longer DNA/RNA fragments compared to other sequencers, such as Illumina, etc. This nanopore method provides distinct advantages for generating complete genome assemblies from microorganisms. Specifically, the R9.4 flow cells used for MinION sequencing have much lower error rates compared with earlier versions of the ONT platform. Coupled with base calling using Dorado software, higher-quality long reads can now be generated for complete bacterial genome assembly. In this chapter, we describe a detailed MinION method to assemble a complete genome from a microorganism, polish the final assembly, and evaluate the genome quality using various software tools. Because of the low cost for MinION sequencing, this platform could be an asset for virtually any laboratory interested in generating complete genomes from microorganisms.

Genome, Bacterial↗