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At least 253 records · Page 14Linked to original sources
PubMed1980

Influence of sex, developmental time and food on beta-N-acetylglucosaminidase activity in the rice weevil Sitophilus oryzae L.

The effects of some biological parameters on beta-N-acetylglucosaminidase activity have been investigated in S. oryzae. There is no significant influence of sex and developmental time on the enzyme activity level, which appears in contrast to be greatly influenced by food (wheat or sorghum). Sorghum contains competitive inhibitors which are almost completely removed after dialysis. Fasting relieves this inhibition very quickly, suggesting that inhibitors act directly at the gut level.

Acetylglucosaminidase↗
PubMed2005

A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil.

A reproducible Agrobacterium tumefaciens-mediated genetic transformation method that delivers fertile and morphologically normal transgenic plants was developed for cultivated tepary bean (Phaseolus acutifolius L. Gray). Factors contributing to higher transformation efficiencies include (1) a low initial concentration of bacteria coupled with a longer cocultivation period with callus, (2) an initial selection of callus on a medium containing low levels of the selectable agent, (3) omission of the selectable agent from the medium during callus differentiation to shoots and (4) the efficient conversion of transgenic shoots into fertile plants. All plants regenerated with this procedure (T0) were stably transformed, and the introduced foreign genes were inherited in a Mendelian fashion in most of the 33 independent transformants. Integration, stable transmission and high expression levels of the transgenes were observed in the T1 and/or T3 progenies of the transgenic lines. The binary transformation vectors contained the beta-glucuronidase reporter gene, the neomycin phosphotransferase II selectable marker gene and either an arcelin 1 or an arcelin 5 gene. Arcelins are seed proteins that are very abundant in some wild P. vulgaris L. genotypes showing resistance to the storage insect Zabrotes subfasciatus (Boheman) (Coleoptera, Bruchidae). Transgenic beans from two different cultivated P. acutifolius genotypes with high arcelin levels were infested with Z. subfasciatus, but they were only marginally less susceptible to infestation than the non-transgenic P. acutifolius. Hence, the arcelin genes tested here are not major determinants of resistance against Z. subfasciatus.

Agrobacterium tumefaciens↗
PubMed2001

Anoxia tolerance and anaerobic metabolism in two tropical weevil species (Coleoptera, Curculionidae).

Although the two curculionid beetle species Cosmopolites sordidus and Temnoschoita nigroplagiata are found in the same habitat (banana plantation), they differ with respect to their microhabitat preference and thereby in their risk of being submerged after rain. The physiological characteristics of the two species that might be important in this context were investigated. As expected, C. sordidus is more resistant to submergence (faster recovery, lower mortality: 30% after 9 days submergence at 20 degrees C); this can be attributed to a generally lower metabolic rate, higher glycogen reserves (135 micromol glycosyl units x g FW(-1)) and a moderate lactate production under anoxia. In T. nigroplagiata, the glycogen reserves are almost completely depleted after 1 day submergence at 20 degrees C and a higher proportion of this glycogen can recovered as lactate (16%). During submergence, the adenylate energy charge falls in both species to 0.2 or below, whereas the total adenine nucleotide content decreases only slowly, especially in C. sordidus.

Adaptation, Physiological↗