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At least 253 records · Page 14Linked to original sources
PubMed1997

Immunochemical and immunohistochemical study of the 27- and 29-kDa calcium-binding proteins and related proteins in the porcine tooth germ.

Our previous report identified 27- and 29-kDa calcium-binding proteins in porcine immature dental enamel. In this study we revealed that the N-terminal amino acid sequences of the two proteins were identical: LLANPXGXIPNLARGPAGRSRGPPG. The sequence matches a portion of the amino acid sequence of the porcine sheath protein, sheathlin. Porcine tooth germs were investigated immunochemically and immunohistochemically using specific antibodies raised against synthetic peptide that included residues 13-25 of this sequence. The affinity-purified antibodies reacted with several proteins extracted from newly formed immature enamel in immunochemical analyses, especially protein bands migrating at 62, 35-45, 29, and 27 kDa in SDS-polyacrylamide gels. The largest protein detected was a weak band near 70 kDa. In immunochemical analyses of proteins extracted from the inner (old) immature enamel, the antibody reacted faintly with the 27- and 29-kDa proteins. In immunohistochemical preparations, the Golgi apparatus and secretory granules of the secretory ameloblast, and the surface layer of immature enamel showed immunoreactivity. The immunoreactivity of immature enamel just beneath the secretory face of the Tomes' process was intense. No immunoreactivity was found in the Golgi apparatus of the maturation ameloblast. These results suggest that the 70-kDa protein, whose degradation might be very fast, is the parent protein of the 27- and 29-kDa proteins.

Ameloblasts↗
PubMed1985

Effects of orally-administered caffeine on the growth of tooth germs in protein-energy malnourished newborn rats.

Lactating dams with 8 suckling pups were fed either a 6 or 20 per cent protein diet from the birth of the pups to day 15. Pups were divided into caffeine and non-caffeine groups and, every other day between days 3 and 13, were either intubated with caffeine (1 mg/100 g body wt) dissolved in 0.1 ml physiological saline solution, or 0.1 ml saline solution as a control. At day 15, the weight of incisors and molar germs, hydroxyproline content, collagen synthesis and calcium contents were determined. Nutritional factors exercised consistent effects on all the parameters, but effects attributable to caffeine intubation were minor. There was lower molar weight in the 20 per cent protein group with caffeine, and collagen synthesis of incisor and molar germs in the caffeine and non-caffeine animals in the 6 and 20 per cent group were different. These parameters showed a significant interaction between nutrition and caffeine. Thus the direct administration of a small amount of caffeine to newborns causes only slight effects on tooth-germ development under some nutritional conditions.

Administration, Oral↗
PubMed1989

The effects of cadmium on the p-nitrophenyl phosphatase and inorganic pyrophosphatase activities of alkaline phosphatase in developing hamster tooth germs.

p-Nitrophenyl phosphatase (p-NPP-ase) and inorganic pyrophosphatase (PPi-ase) activities originate from the same alkaline phosphatase enzyme. Only the PPi-ase site has zinc (Zn2+) as a cofactor. Cadmium (Cd2+) in concentrations from 10(-5) mol/l upwards inhibited the PPi-ase activity, but did not inhibit the p-NPP-ase activity at all. In mineralizing tooth germs Cd2+ may replace Zn2+, thereby changing the specific stereoconfiguration in the active centre needed for PPi-ase activity, but not that for p-NPP-ase activity.

4-Nitrophenylphosphatase↗
PubMed1990

[Effect of cyclophosphamide on the development of incisor tooth germs in mice].

Thirty six female mice were injected on the 12th day of the gestational period with 0.2 ml of distilled water (control group) or of an acqueous solution containing either 30 mg/Kg or 50 mg/Kg of body weight of cyclophosphamide (treated group). The animal were killed at 24, 48, 72, 96 and 120 hours after the injection and 3 days after birth. It was verified that cyclophosphamide interferes on the tooth germ development and that this effect is in directly ratio of the doses used.

Animals↗
PubMed1988

Development of a tooth germ within an autogenous clavicular graft.

The left side of the mandible distal to the second primary molar of a 3 years old Southern Chinese boy was resected as a result of osteomyelitis. A diaphyseal clavicular graft pedicled on the sternomastoid muscle was selected for the reconstruction of the mandible. By the age of 6 years, the mandible although stunted in size did exhibit appropriate remodelling. In addition a tooth germ in the region of the second permanent molar was found developing in the autogenous bone graft of the mandible.

Bone Transplantation↗
PubMed2004

Apoptosis distribution in the first molar tooth germ of the field vole (Microtus agrestis).

Apoptosis represents an important process in organ and tissue morphogenesis and remodeling during embryonic development. A role for apoptosis in shape formation of developing teeth has been suggested. The field vole is a useful model for comparative studies in odontogenesis, particularly because of its contrasting molar morphogenesis when compared to the mouse. However, little is known concerning apoptosis in tooth development of this species. Morphological (cellular and nuclear alterations) and biochemical (specific DNA breaks--TUNEL staining) characteristics of apoptotic cells were used to evaluate the temporal and spatial occurrence of apoptosis in epithelial and mesenchymal tissues of the developing first molar tooth germs of the field vole. Apoptotic cells were found in non-proliferating areas (identified previously) throughout bud to bell stages, particularly in the epithelium, however, scattered also in the mesenchyme. A high concentration of TUNEL positive cells was evident in primary enamel knots at late bud stage with increasing density of apoptotic cells until ED 16 when the primary enamel knot in the field vole disappears and mesenchyme becomes protruded in the middle axes of the bell forming two shallow areas with zig-zag located secondary enamel knots. Distribution of TUNEL positive cells corresponded with localisation of secondary enamel knots as shown using histological and 3D analysis. Apoptosis was shown to be involved in the first molar development of the field vole, however, exact mechanisms and roles of this process in tooth morphogenesis require further investigation.

Animals↗
PubMed1995

Relation between the trans-Golgi network and the Golgi stack on development of the Golgi apparatus of the ameloblast in developing rat molar tooth germs.

BACKGROUND: The problem of how the functional compartments of the Golgi apparatus organizes during cell differentiation to become a well-formed Golgi apparatus is as yet an unresolved issue. This study was designed to define the involvement of the trans-Golgi network (TGN) and the Golgi stack in organizing the Golgi apparatus. METHODS: The distribution of the TGN marker enzyme was examined in the ameloblast of developing rat molar tooth germs using cytochemistry with Co-enzyme A phosphatase (CoA Pase) and cytidine monophosphatase (CMPase). RESULTS: Typically formed Golgi apparatus was observed in the secretory ameloblast but not in the presecretory ameloblast. Organization of the Golgi apparatus through the presecretory ameloblast was noted. In the presecretory ameloblast, Golgi stacks of different sizes and clusters of small vesicles were located in the cytoplasm lateral to the nucleus. The saccules with enzymes marked for TGN were also observed in the cytoplasm lateral to the nucleus. These saccules were adjacent to the cluster of small vesicles and/or the Golgi stack. Upon cell differentiation, Golgi stacks were seen in line along the long axis of the cell, and the file of the stacks in the cytoplasm lateral to the nucleus was formed. The positive saccule was seen in a parallel line equal to the length of the Golgi stacks. CONCLUSIONS: In organizing the Golgi apparatus, the development process of the TGN and the Golgi stack appear to be different, and new Golgi stacks seem to be formed through the accumulation of small vesicles near the pre-existing TGN.

Ameloblasts↗