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PubMed1993

Correlation of ultrasound assessment of endometrial growth and plasma steroid concentrations during superovulation for in vitro fertilization.

Endometrial and follicular development were investigated by ultrasound in 25 normally cycling women who received clomiphene citrate and human menopausal gonadotrophin to induce multiple follicular development for in vitro fertilization. Ultrasonic determination of endometrial thickness and reflectivity grading and follicular number were correlated with daily concentrations of estradiol (E2) and progesterone (p) in peripheral serum. Serum E2 showed a better positive correlation with endometrial thickness than with total number of developing follicles. There was a significant inverse correlation between endometrial thickness and plasma p concentration. The E2 value per follicle and E2/p ratio were both weakly correlated respectively with endometrial thickness. These data indicate that ultrasound determination of endometrial thickness is a useful ultrasonic parameter for monitoring ovarian function for in vitro fertilization. However, endometrial thickness should always be combined with total number of developing follicles, in order to reach decisions concerning timing of oocyte recovery.

Adult↗
PubMed1994

The effect of the phase of the menstrual cycle and the birth control pill on athletic performance.

Investigators are not in agreement on the effects of either the phase of the menstrual cycle, or the administration of OCAs on athletic performance. It appears, however, that apart from subtle changes in some variables, for most women there is no significant effect. Medals have been won and world records set in any phase of the menstrual cycle, and also by women taking OCAs. In terms of documentation of cycle phase, newer hormonal measurement techniques such as the levels of urinary luteinizing hormone (LH) to detect ovulation or salivary progesterone, should make it easier in the future to obviate the methodologic difficulties encountered in earlier studies. Further studies should also focus on the midcycle estradiol surge as well, in order to determine the relative contributions of estrogen and progesterone to any observed performance changes. Given the possibility that some cardiovascular, respiratory, and metabolic variables may change slightly during the course of a regular ovulatory menstrual cycle, it behooves researchers who are using women as subjects in other types of studies to standardize the menstrual cycle phase in which they are tested, in order to eliminate any possible confounding effects due to hormonal variation. Regarding the effects of oral contraceptives on performance, any conclusions from the studies to date are complicated by the proliferation of preparations currently on the market. Further studies are needed on monophasic, biphasic and triphasic formulations, including OCAs with the newer progestins (desogestrel, gestodene and norgestimate), as well as the progesterone-only agents (both oral and injectable). Prospective double blind randomized studies must be done, using a proper control group. The difficulty with this technique, however, is that women in the control group will inevitably be in various phases of the cycle, so accurate hormonal documentation is also essential in order to correctly interpret the findings. Just as the past few decades have seen a significant advancement in the participation of women in sports, future years should bring an enhanced scientific knowledge base about the interactions of the special hormonal considerations of the exercising woman throughout her reproductive life cycle.

Adult↗
PubMedDate not supplied

[In assisted reproduction technology there is not systemic age limitation].

The age limit imposed upon women who which to undergo ART is unfair. Of course success rates decrease strongly for women over 40, and women must be informed of it. But if the ovarian reserve of these women is normal, and if they answer correctly to ovarian stimulation, there is no good reason to refuse them ART.

Adult↗
PubMed1994

Re-examining steroid hormone metabolites as ovulation markers using monoclonal antibodies.

Plasma progesterone and plasma estradiol levels are commonly used to monitor ovulation in women although for the adequate documentation of ovulation the expense and discomfort of multiple venipuncture sampling may be required. Accurate and definitive information on ovulation in women can be obtained by the simple measurement of metabolites of progesterone and estradiol in early morning urine samples. These analyses have been made possible by the use of our own highly specific monoclonal antibodies to both pregnanediol-3-glucuronide (P-3-G) and estrone-3-glucuronide (E-3-G) and the development of simple, direct, automated enzyme-linked immunosorbent assays. Sequential sampling and the generation of ovulation profiles allows detection of ovulation and identification of the infertile/fertile phases of the cycle for either planned pregnancies or natural family planning. Aberrations in ovulation are easily detected as is documentation of the transition to menopause. The use of sequential, spaced, early morning urine samples for P-3-G and E-3-G allows accurate assessment of ovulatory function rather than relying on the usual single plasma sampling. The data presented also show that the direct determination of plasma pregnanediol-3-glucuronide in plasma is as informative as plasma progesterone measurement.

Adult↗
PubMed1991

Comparison of a rapid, quantitative and automated assay for urinary luteinizing hormone (LH), with an LH detection test, for the prediction of ovulation.

The prediction of ovulation is necessary for oocyte aspiration in a spontaneous cycle and can be reliably achieved only by measuring luteinizing hormone (LH). Since radioimmunoassays of LH take too long for repeated measurements on the same day, we evaluated the possibility of adapting a rapid and fully automated assay of serum LH for use with urine samples. The study group comprised spontaneously ovulating women (38 cycles) who requested artificial insemination. Their serum oestradiol (E2) levels, ultrasound profile (US) and thrice daily urinary LH levels were determined from day 10 of their menstrual cycle. These patients were followed until US signs of follicular rupture were recorded. In all patients, a well-defined LH peak was measured in the urine. This peak lasted 12-15 h and was followed in 35 cycles (no US available for 3) by follicular rupture 9-51 h later. The data were grouped according to the time of the LH peak on day 0. Patients experiencing an LH peak between 0300 h and 0700 h on day 0 had significantly lower levels of E2 on day 0 compared to those with an LH peak between 2200 h and midnight. This is due to the fact that in the patients with an LH peak between 0300 h and 0700 h, E2 levels were already decreasing (from day 1 to day 0), whereas in those with the LH peak between 2200 h and midnight E2 levels were still increasing on the morning of day 0. We conclude that the 30-min IMX LH assay is a reliable, rapid and readily acceptable method for measuring urinary LH and for the prediction of ovulation.

Adult↗
PubMed1983

Reproductive traits of Holsteins and Jerseys. Effects of age, milk yield, and clinical abnormalities on involution of cervix and uterus, ovulation, estrous cycles, detection of estrus, conception rate, and days open.

Two hundred and twelve Holstein and Jersey cows were in a study to determine factors that affected reproductive traits. First ovulation occurred about 3 wk postpartum, and interval to first ovulation was greater in cows that had clinical abnormalities postpartum than in normal cows. Jerseys producing more milk ovulated sooner postpartum than lower producing herdmates. Involution of cervix and uterus occurred later postpartum in cows that had clinical problems postpartum. Involution of genital tract occurred later postpartum in older cows and sooner postpartum in cows that had higher milk yields. Duration of first postpartum estrous cycle was 4 days less than for second postpartum cycle. Percentages of estrous cycles detected by standing estrus were 43 and 73% for Holsteins and Jerseys. Estrous detection rates were highest for cows that produced slightly above the mean milk yield and did not differ between cows in highest and lowest milk production quartiles. First detected estrus and days to first insemination occurred later postpartum in Holsteins as milk yield deviation from herdmates increased, regardless of sign. In Jerseys, days to first insemination and days open increased linearly as milk yield increased. Days to first insemination and conception were greater in cows with postpartum clinical problems. Conception rate at first insemination postpartum increased in proportion to concentration of progesterone in blood samples collected during 12 days before first insemination. Overall, clinical problems at parturition and postpartum lowered reproductive performance in both breeds. There was a slight antagonism between milk yield and reproductive performance (days open) in Jerseys but not in Holsteins.

Age Factors↗
PubMed1995

[Value of the Clearplan Ovulation Test in sterility treatment].

Detection of ovulation is an essential step in sterility treatment. Determination of the luteinising hormone is the most reliable method, which is generally performed by serum analysis. In this study, we proved the reliability of LH analysis in urine. 44 cycles of 35 volunteers with regular ovulatory cycles were examined. From day 11 on, daily LH measurements were performed on serum and urine. Ovulation was established by ultrasound monitoring of follicular development and/or by progesterone detection in the luteal phase. The sensitivity was 89% for serum analysis and 97.5% for urine analysis. The specificity was 100% for both methods. Hence, LH detection in urine is a reliable and secure method for ovulation determination. As an advantage over serum analysis, it requires less consultations, thereby offering a less expensive and stress reduced treatment.

Adult↗
PubMed1987

Correlation between the endocrine profile of ovulation and the ultrasonically detected 'double contour' of the preovulatory follicle.

The exact prediction of ovulation is becoming more important in the management of infertile couples. The routine methods used to monitor ovulation are plasma levels of estradiol and luteinizing hormone, and ultrasonic scanning of graafian follicle diameter. Ovulation is known to occur at a wide range of follicular diameters and it cannot be used as a single predictor of ovulation. In a previous study we described the preovulatory double contour of the dominant follicle occurring a few hours before ovulation. The purpose of this study was to correlate this sign with the hormonal profile to determine whether it can be used as a single parameter in predicting ovulation. The results demonstrate a close relationship between the LH peak and the appearance of the double contour which can be used as a reliable predictor of impending ovulation.

Estradiol↗
PubMed1993

Characterization of the periovulatory period in superovulated heifers.

Heifers (n=31) were superovulated with an FSH-P/cloprostenol regimen, and at 12 and 24 hours after the onset of estrus they were inseminated. Blood sampling for LH analyses and ultrasound scanning of the ovaries were performed at 4-hours intervals. The scanning, at which the first and last ovulations were recorded, was performed at 22.7 +/- 1.5 (mean +/- SD) and 31.0 +/- 1.5 hours after the LH peak, respectively. An average of 7.8 +/- 1.0 ovulations was monitored when the first ovulations were detected, while 2.8 +/- 0.7 ovulations occurred later. At 16 hours after detection of the first ovulations the oviducts were flushed and 5.6 +/- 0.5 fertilized and 2.3 +/- 0.3 unfertilized ova were isolated per animal. The fertilized ova displayed spherical pronuclei of synchronous development, and polyspermic penetration was not seen. At 24 hours after detection of the first ovulations the content of the remaining 3.3 +/- 0.5 nonovulatory follicles > 8 mm per animal was aspirated. Expanded cumulus investment was found in 69.4% of the oocytes, while 22.4% had abstricted the first polar body.

Journal Article↗
PubMed1991

The effects of dietary protein on age and weight at the onset of puberty in Brown Swiss and Zebu heifers in the tropics.

The effects of dietary protein level on age and weight at onset of puberty were studied in the Mexican tropics using 12 Brown Swiss and 12 Zebu heifer calves in a completely randomized block design. The calves were weaned at 3 mo of age. They were confined in individual pens and fed either an adequate protein (12.8%) or a high protein (16.4%) diet ad libitum through the onset of puberty, which was defined as the first ovulation. To detect the first ovulation, heifers were palpated at 8 mo of age and then every 2 wk. At the detection of a developing follicle or any behavioral sign of estrus, they were palpated daily. Ovulation was confirmed by palpation of a corpus luteum. A blood sample for the determination of plasma progesterone concentration was taken with each palpation. There was a significant effect of breed on age at the onset of puberty, which was 9.4 and 12.3 mo for the Brown Swiss and Zebu heifers, respectively. Weight at puberty was 233 kg, independent of breed. Dietary protein had no effect on the onset of puberty. Brown Swiss heifers reach puberty earlier than Zebu heifers. Heifers in the tropics fed according to NRC recommendations reach puberty at ages comparable to those in temperate climate. This suggests that, if managed properly, calving age for Brown Swiss and Zebu heifers can be 24 and 27 mo, respectively. This is a considerable improvement from 36 to 48 mo of age now common in the area of study.

Journal Article↗
PubMed2005

Transient expression of CC chemokine TECK in the ovary during ovulation: its potential role in ovulation.

PROBLEM: Chemokine thymus-expressed chemokine (TECK), which is expressed exclusively in the thymus and small intestine, plays a critical role in T-cell development. Our previous study revealed its expression in the ovary also. This study investigated its ovarian expression during ovulatory process. METHOD OF STUDY: Super-ovulation was induced in young female CD1 mice by equine chorionic gonadotropin (eCG) and human chorionic gonadotropic (hCG). Ovarian TECK expression during ovulation was determined by: (1) reverse transcriptase-polymerase chain reaction (RT-PCR) at mRNA level, (2) Western blot and immunohistology at the protein level, and (3) leukocyte infiltration assay at the bioactive level. RESULTS: A transient, high-level expression of TECK in murine ovaries at the mRNA level during hCG-induced ovulation was detected. Sequencing of directly cloned PCR product confirmed the ovarian expression of TECK. The peak expression of TECK was observed at 10-12 hr post-hCG injection; real-time PCR revealed an 800-fold increase during its expression peak over 0 hr. The expressed ovarian TECK protein was readily detectable by Western blot. Immunohistochemistry localized TECK expression to the ovarian interstitial tissue surrounding, or in the theca layer of the mature follicles undergoing ovulatory process. Expression of TECK receptor, the CC chemokine receptor (CCR9) was also detected in the ovulating ovaries. Using in vitro leukocyte infiltration assay, we first demonstrated that ovaries undergoing the ovulatory process were able to selectively chemoattract mononuclear cells. Importantly, neutralization of TECK by the antibody resulted in a 85% reduction in the chemotactic activities of the ovaries. CONCLUSION: This study suggested that ovarian expression of TECK is under a tight hormonal regulation, and expressed TECK may be responsible for recruitment of mononuclear cells into the ovary to participate in the ovulatory process.

Animals↗
PubMed2003

Follicular size and response to Ovsynch versus detection of estrus in anovular and ovular lactating dairy cows.

In a commercial dairy herd, 316 lactating Holsteins were studied to determine the percentage of anovular cows, to examine follicular sizes in anovular cows, and to compare synchronized ovulation (Ovsynch) versus detection of estrus on fertility of ovular and anovular cows. Ultrasonography examinations at 47 to 53 d and at 54 to 60 d postpartum were used to measure follicles and to classify cows as ovular or anovular. Anovular cows were identified as those with no detectable luteal tissue by ultrasonography and by low progesterone in blood samples collected weekly. Anovular cows included 28% of 122 primiparous cows and 15% of 194 multiparous cows. Of 64 anovular cows, 20% had follicles > or = 25 mm that might be considered cystic (4% of total cows), 58% had 15- to 24-mm follicles, and 22% had 9- to 14-mm follicles. Cows identified as ovular and anovular were randomly assigned within cyclic status to one of two artificial insemination (AI) strategies: 1) AI after detected estrus during 21 d, or 2) timed AI after a 10-d Ovsynch protocol. Weekly ultrasonography continued for 21 d to detect ovulations. For the Ovsynch sub-groups, 97% of ovular and 94% of anovular cows ovulated after the second GnRH injection. Within 21 d, spontaneous ovulations for the detection of estrus sub-groups were 42% of anovular cows vs. 89% of ovular cows. Conception rates were greater for ovular cows regardless of treatment, but conception rates between respective Ovsynch and estrus detection groups for ovular (32%, 35%) or anovular (9%, 11%) cows were similar. Although 20% of lactating cows were not cyclic by about 60 d postpartum, nearly all ovulated following Ovsynch. However, anovular cows had lower conception than ovular cows whether inseminated after detected estrous or after Ovsynch.

Animals↗
PubMed2006

Using cervical mucus and other easily observed biomarkers to identify ovulation in prospective pregnancy trials.

To help fulfil the objectives of prospective studies of pregnancy and human development, such as the National Children's Study, there is a need to pinpoint ovulation and conception in a cohort of women. This paper discusses the suitability of using natural and easily observed markers of ovulation for detection of the most likely day of ovulation and conception. Of the biomarkers supported by clinical data, the use of cervical mucus is emphasised as it appears most promising. A considerable amount of data suggests that observation of changes in vaginal discharge from cervical mucus can closely approximate the day of ovulation, is easy to learn and suitable for a large group of women. While this approach could also be cost-effective, a significant drawback may be the learning phase which may take 2-3 instructional sessions for women to fully identify recurring changes across the menstrual cycle.

Biomarkers↗
PubMed2006

Successful timing of ovulation using deslorelin (Ovuplant) is labour-saving in mares aimed for single ai with frozen semen.

To minimize the number of matings/inseminations, controlled ovulation has been practised since a long time ago. A potent short-term implant, releasing the GnRH analogue deslorelin (Ovuplant((R))) has been used in Australia and North America for several years for hastening the ovulation time in mares, but the product is not registered on the European market. This study was aimed to investigate: (1) ovulation time in mares implanted with Ovuplant when the largest follicle was 42 mm or more in size, (2) repeatability of ovulation time in successive oestruses when treated with Ovuplant, (3) pregnancy rate after single insemination with frozen-thawed semen near ovulation. This study included 11 mares, and altogether 17 timed ovulations. Follicular growth and ovulation were determined by palpation per rectum and by ultrasonography in the morning (at 7:00 hours) every second day until observation of a follicle of at least 42 mm in diameter. Then the mares were re-examined in the afternoon (at 19:00 hours), and an Ovuplant was inserted in the mucosa of the vulva. For detection of ovulation, the mares were palpated and ultrasounded repeatedly from 36-42 h after the insert. The mares were inseminated with frozen-thawed semen once at ovulation. All mares ovulated at 36-48 h after treatment and 94% at 38-42 h after treatment. The six mares that were treated at two oestruses ovulated at 39.9 and 39.7 h, respectively. Five of 11 mares (45.4%), inseminated with frozen-thawed semen at the first oestrous cycle were pregnant day 14-16 after ovulation. Using this protocol, there is no need of palpation/ultrasonography during night hours, and examination at 36 and 41 h after implantation might be enough for estimation of ovulation time.

Animals↗