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Thin-layer chromatography-densitometry and liquid chromatography analysis of alkaloids in leaves of Papaver somniferum under stress conditions.

The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accuracy of the thin-layer chromatography method for quantitative analysis is limited. Without purification of samples the background is too noisy. Column liquid chromatography is a sensitive and relatively inexpensive method that allows precise quantitative determination of the alkaloid content.

Alkaloids↗
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Metoclopramide and ureteric colic.

The pain-reducing property of metoclopramide (Primperan) was compared with that of a narcotic combination drug (Spasmofen) in a double-blind study on 40 patients with ureteric colic. The tested drugs had equal pain-reducing capacity and no serious side-effects were noticed. Metoclopramide appears to be an alternative when inhibitors of prostaglandin synthesis or narcotics are contraindicated.

Adult↗