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Effects of a broad spectrum and biorational insecticides on parasitoids of the Nantucket pine tip moth (Lepidoptera: Tortricidae).

We examined effects of aerial application of acephate (Orthene), Bacillus thuringiensis variety kurstaki Berliner (Foray), and tebufenozide (Mimic) on larval/pupal parasitoids of the Nantucket pine tip moth, Rhyacionia frustrana (Comstock), in the southwestern Georgia coastal plain. Parasitism of tip moths in acephate-treated plots was significantly lower than in untreated plots. Bacillus thuringiensis and tebufenozide showed no significant effects on parasitism. A tachinid, Lixophaga mediocris Aldrich, comprised a significantly greater proportion of emerging parasitoids in acephate-treated than in untreated control plots, whereas a chalcidid, Haltichella rhyacioniae Gahan, was less abundant in the acephate-treated plots. Acephate has a negative, but somewhat species-specific, impact on tip moth parasitism.

Animals↗

Susceptibility of male oriental fruit moth (Lepidoptera: Tortricidae) populations from New Jersey apple orchards to azinphosmethyl.

Toxicological responses to azinphosmethyl of male Oriental fruit moth, Grapholita molesta (Busck), from five commercial orchards in which control failures had occurred were examined for azinphosmethyl resistance and for potential resistance mechanisms by using topical pheromone trap bioassay and compared with a reference population that had no history of control failure and had received little selection pressure. The 1998 field survey indicated moderate level of resistance to azinphosmethyl (2.7-4.1-fold); slopes of regressions lines (2.47-2.76) indicate genetically heterogeneous populations. An approximate twofold decline was observed between the fourth flight of 1998 and the first flight of 1999, suggesting the presence of unstable resistance in moths collected from these study sites. The 1999 field surveys indicated lower levels of tolerance to azinphosmethyl. The resistance ratios ranged from 1.17 to 1.86 during the first flight of 1999 and 1.24-2.64 during the fourth flight of 1999. Steep slopes of the concentration-response lines during 1999 season indicated the presence of genetically homogeneous populations with some exceptions. A 1.5-2.0-fold increase was observed between the first and fourth flights of 1999, indicating that resistance can build up during the growing season. S,S,S,-tri-n-butyl phosphorotrithioate (DEF), but not piperonyl butoxide, significantly enhanced the toxicity of azinphosmethyl, suggesting that enhanced metabolism by esterases is involved in the tolerance of azinphosmethyl in moths collected from these study sties.

Animals↗

Integrated control of oriental fruit moth (Lepidoptera: Tortricidae) in peach orchards using insecticide and mating disruption.

The efficacy of an integrated and a conventional oriental fruit moth, Grapholita molesta (Busck), control program was compared using 4-ha blocks of peach at three Niagara Peninsula farms during 1997-1999. In the integrated program, chlorpyrifos was used to control first-generation larvae and mating disruption using Isomate M100 pheromone dispensers was used to control the second and third generations. In the conventional program, chlorpyrifos was used to control first-generation larvae and pyrethroids were used to control larvae of the later generations. The average release rate of pheromone was 23.7-26.4 mg/ha/h over a period of 86-91 d. The pheromone treatment reduced the capture of moths in pheromone-baited traps on average by 98%, suggesting a high level of disruption. The integrated program provided control of oriental fruit moth similar to the control provided by a conventional program. The mean percentage of peach shoots infested with first- and second-generation larvae, and fruit infested with third-generation larvae was not significantly greater in the integrated-program blocks during the 3-yr study. The elimination of insecticide sprays from the integrated-program blocks did not result in an increase in damage caused by plant bugs. The incidence of damage caused by other pests was negligible in both the integrated and conventional blocks.

Animals↗

Effect of cold treatment on survival and development of codling moth (Lepidoptera: Tortricidae) in cherry.

'Bing' cherries, Prunus avium L., were obtained from an organic orchard and a conventional commercial orchard. The two groups were examined separately in replicated tests infested with each fruit initially infested with a first-instar codling moth, Cydia pomonella (L.). To simulate commercial postharvest holding conditions, the treatments were 0 (control), 1, 2, 4, 7, 10, and 14 d cold storage at 3.3 degrees C. The fruits were examined three or more times to determine larval survival, life stage, fruit condition, and fungal disease. Survival of first instars was affected only by cold storage durations of > or = 7 d. When infested with codling moth larvae, both organic and conventionally grown cherries quickly deteriorated from fungal diseases. The rate of moth development was estimated from the surviving larvae and was significantly different between organic and conventionally grown cherries for all instars except the second.

Animals↗

The cost of slowing the spread of the gypsy moth (Lepidoptera: Lymantriidae).

Beginning in 1992, the Slow The Spread (STS) pilot project was initiated to target gypsy moth (Lymantria dispar L.) spread rate reduction by controlling populations in the transition zone. The project uses intensive monitoring techniques, with pheromone-baited sticky moth traps, to detect low-level populations and target them for eradication. The primary objective of the pilot project was to evaluate the feasibility of using integrated pest management techniques to slow the spread of gypsy moths over a large geographical area. In this study, the cost of STS pilot project activities in 1993-1995 was investigated. A cost accounting system was developed and used as a framework to collect the cost data and to investigate cost patterns and characteristics. Total expenditures of STS activities for 1993-1995 were 7,685.2 million dollars. Per unit cost was 49.67 dollars per trap with the direct cost component being 35.03 dollars per trap. Trapper labor and vehicle expense accounted for approximately 90% of this direct cost. Per unit cost for treatment activities was found to average 27.86 dollars per treated acre. In general, the STS pilot project is labor intensive, specifically the trapping component. From 1993-1995, 59% of total project expenditures were spent on trapping activities, 28% on pesticide treatments, and 13% on data management. A trapper productivity rate regression model is described.

Animals↗

Effect of rearing strategy and gamma radiation on field competitiveness of mass-reared codling moths (Lepidoptera: Tortricidae).

We compared the field competitiveness of sterile codling moth, Cydia pomonella (L.), males mass-reared through diapause or standard production protocols and treated with either 150 or 250 Gy of gamma radiation. Evaluations were performed during spring and summer 2003 by using field release-recapture tests. Released males were recaptured using traps baited with synthetic pheromone or with virgin females. In addition, mating tables baited with virgin females were used in the summer to assess the mating competitiveness of the released moths. Field performance of released males was significantly improved by rearing through diapause and by lowering the dose of radiation used to treat the insects. These effects were observed during spring when evening temperatures were relatively cool and in summer when evening temperatures were high. These effects were observed regardless of the sampling method (i.e., capture in pheromone-baited traps, virgin female-baited traps, or in mating tables). There were significant interactions between larval rearing strategy and radiation dose with respect to day of recapture. The effect of rearing strategy on male performance was observed immediately after release, whereas the effect of dose of radiation was usually delayed by 2-3 d. In general, the best treatment for improving codling moth male field performance was a combination of rearing through diapause and using a low dose of radiation (150 Gy). The difference in performance when insects were treated with 150 or 250 Gy was greater when males had been reared using standard (nondiapause) rearing protocols, suggesting that diapause rearing may attenuate some of the negative effects of the higher doses of radiation.

Animals↗

Impact of ant predation and heat on carob moth (Lepidoptera: Pyralidae) mortality in California date gardens.

Dates, Phoenix dactylifera L., undergo a natural fruit abscission during the summer in California date gardens. Many of the abscised dates become lodged in the date bunch, and we demonstrated that carob moth, Ectomyelois ceratoniae (Zeller), prefer to use these dates as a reproduction host compared with dates that fall to the ground. We also found that abscised fruit shaken onto the ground had significantly fewer live carob moth larvae than fruit that remained in bunches in the tree. Mortality in the dropped fruit was attributed to predation by two native ant species, the fire ant Solenopsis aurea Wheeler, and the California harvester ant, Pogonomyrmex californicus (Buckley), in concert with extreme summer ground temperatures. Dates that fell in the full sunlight rapidly increased in temperature, which resulted in larvae either exiting the fruit (exposing them to ants) or dying in the fruit. Removal of abscised dates from bunches may provide a possible management strategy for carob moths in California date gardens.

Animals↗

Small plot trials documenting effective mating disruption of oriental fruit moth by using high densities of wax-drop pheromone dispensers.

In 2004 field experiments, we compared the effectiveness of various deployment densities of 0.1-ml paraffin wax drops containing 5% pheromone versus Isomate M-Rosso "rope" dispensers for disruption of Grapholita molesta (Busck). Treatments were evaluated in 0.05-ha (12-tree) plots of 'Delicious' apples receiving regular maintenance according to growers' standards, but not sprayed with insecticides. The application densities of 0.1-ml wax drops were 3 per tree (820/ha), 10 per tree (2,700/ha), 30 per tree (8,200/ha), and 100 per tree (27,300/ha). Wax drops were compared with 3-ml dispensers of pheromone-containing paraffin wax or Isomate M-Rosso ropes at 1.8 per tree (500/ha) and untreated control plots. Treatments were applied before the start of each of three moth generations. Orientational disruption, as measured by inhibition of moth captures in pheromone-baited delta traps, was greatest in plots that received 100 drops per tree (99.2%) and 30 drops per tree (99.4%). More than 55% of tethered, virgin females were mated in control plots after one night of deployment. However, no mating was recorded at the two highest application densities of wax drops where orientational disruption of traps exceeded 99%. Mating ranged from 7 to 20% among the other treatments, including Isomate rope dispensers. G. molesta males were observed closely approaching pheromone dispensers in plots containing ropes and wax drops, documenting competitive attraction between synthetic pheromone sources and feral females. The majority of observed G. molesta males approached within 60 cm of wax drops or pheromone ropes and departed within 20 s by flying upwind. Thirty wax drops per tree yielded higher mating disruption of G. molesta than did Isomate M-Rosso dispensers deployed at the recommended rate of 500/ha (1.8 per tree). Measurement of release rates confirmed behavioral data indicating that paraffin wax dispensers would need to be applied once per G. molesta generation in Michigan. Paraffin wax drops are a promising technology for moth mating disruption. They are cheaper and easier to produce, require less total pheromone per annual application, and produce better mating disruption at appropriate deployment densities compared with Isomate M-Rosso dispensers under high G. molesta population densities. The cost-effectiveness of this approach will require an appropriate mechanized applicator for wax drops.

Animals↗

Higher densities of distributed pheromone sources provide disruption of codling moth (Lepidoptera: Tortricidae) superior to that of lower densities of clumped sources.

Field experiments quantified the effect of synthetic pheromone release-site density and distribution on 1) orientational disruption of male codling moths, Cydia pomonella (L.) (Lepidoptera: Tortricidae), to pheromone-baited traps; and 2) fruit injury. A clustering test varied pheromone release-site density from 0 to 1,000 Isomate-C Plus dispensers per ha while maintaining the total number of dispensers at 1,000. Percentage of orientational disruption of pheromone-baited traps increased significantly as a function of increasing density of release sites. Fruit injury decreased as the density of release sites increased and was lowest in plots treated with Isomate-C Plus dispensers distributed as 1,000 point sources per ha. We also manipulated point source density of 0.1-ml paraffin-wax drops containing 5% codlemone [(E,E)-8,10-dodecadien-1-ol], and thus the total amount of pheromone deployed per hectare. The percentage of disruption of traps baited with either 1.0- or 0.1-mg codlemone lures increased with increasing density of wax drops deployed. Both trapping and field observations confirmed that wax drops were attractive to male codling moths, suggesting that disruption was mediated by competitive attraction. Development of dispensers that can be mechanically applied at high densities has potential to improve the efficacy and economics of codling moth disruption at high population densities.

Animals↗

Hydroprene prolongs developmental time and increases mortality in wandering-phase Indianmeal moth (Lepidoptera: Pyralidae) larvae.

Wandering phase Indianmeal moth, Plodia interpunctella (Hübner), larvae were exposed to the label rate of hydroprene (1.9 x 10(-3) mg [AI] /cm2) sprayed on concreted petri dishes. Larvae were exposed for 1, 3, 6, 12, 18, 24, and 30 h and maintained at 16, 20, 24, 28, and 32 degrees C and 57% RH until adult emergence. Larval developmental time and mortality were significantly influenced by temperature and exposure intervals. Maximum developmental time (47.2 +/- 1.3 d) occurred at 16 degrees C, and the minimum developmental time (7.0 +/- 0.5 d) occurred at 32 degrees C. Larval mortality generally increased at all of the five tested temperatures as exposure period increased. The greatest mortality (82.0 +/- 0.1%) occurred when larvae were exposed for 30 h at 28 degrees C, and minimum mortality (0.0 +/- 0.5%) occurred at 16 degrees C when larvae were exposed for 1 h. The relationships between temperature, exposure period, and developmental time were described by polynomial models, based on lack-of-fit tests. Hydroprene has potential to be an effective alternative to conventional insecticides in surface treatments for Indianmeal moth management. Response-surface models derived from this study can be used in simulation models to estimate the potential consequences of hydroprene on Indianmeal moth population dynamics.

Animals↗

Space travel shortens diapause in gypsy moth eggs.

Field-collected and laboratory-reared gypsy moth eggs were exposed to microgravity, cosmic radiation, sub-freezing temperatures, unusual vibrations, and other extraterrestrial phenomena while they were sealed for 6 days, in January, in a Get-Away-Special (GAS) canister in the open bay of a NASA earth-orbiting spacecraft, the Columbia. Insects were not exposed to light after preparation for and during space flight. Under field conditions, out-of-doors, the eggs should have hatched in April, after 3-4 months of chilling temperatures and should not have hatched after the 6 days of chilling to -11 degrees C during flight in the Columbia spacecraft. However by April 1, more than 4000 larvae had hatched from eggs that had travelled in space, as opposed to approximately 350 from a similar number of control, earthbound eggs. These results indicate that the period of a circannual rhythm in field- and lab-reared insects had been shortened, presumably as result of exposure to microgravity, other factors associated with space flight, and/or conditions of outer space. These results suggest that it may be possible to develop methods for rearing the gypsy moth year round, without the necessity of three months chilling interspersed in the development process. This, in turn, would facilitate production of large numbers of insects for sterile male release or for use as a rearing medium for parasites, predators and pathogens of the gypsy moth.

Animals↗

[Optimization model of spatial population structure: example of poplar moth laying eggs on leaves].

The authors analyze spatial distribution and survival of populations of poplar moth Litchcolletis populifoliella Tr. on its feeding plant--balsam poplar Populus balsamifera. Imago of the moth glue its eggs on the leaves thus determining the future location of their offspring on the host plant. Spatial distribution of eggs on leaf surface and distribution of leaves according egg numbers are not random. On the short distance from each egg the average number of eggs is less, than it should be in case of random distribution. While this distance increases up to some particular value the occurrence of eggs is higher than random. Thus, the eggs of moth are located by groups on the leaf surface. Within each group eggs are situated not very close to each other, this allowing larvae to lower competition for common resource. It is suggested that on the same feeding plant individuals have different interactions: competition, caused by limited quantity of resource and cooperation that is necessary to resist leaf defensive (antibiosis) reaction.

Animals↗

Antennal-specific pheromone-degrading aldehyde oxidases from the moths Antheraea polyphemus and Bombyx mori.

Female moths produce blends of odorant chemicals, called pheromones. These precise chemical mixtures both attract males and elicit appropriate mating behaviors. To locate females, male moths must rapidly detect changes in environmental pheromone concentration. Therefore, the regulation of pheromone concentration within antennae, their chief organ of smell, is important. We describe antennal-specific aldehyde oxidases from the moths Antheraea polyphemus and Bombyx mori that are capable of catabolizing long chain, unsaturated aldehydes such as their aldehyde pheromones. These soluble enzymes are associated uniquely with male and female antennae and have molecular masses of 175 and 130 kDa, respectively. The A. polyphemus aldehyde oxidase has been localized to the olfactory sensilla which contain the pheromone receptor cell dendrites. These same sensilla contain a previously described sensilla-specific esterase that degrades the acetate ester component of A. polyphemus pheromone. We propose that sensillar pheromone-degrading enzymes modulate pheromone concentration in the receptor space and hence play a dynamic role in the pheromone-mediated reproductive behaviors of these animals.

Aldehyde Oxidase↗

The inhibitory effect of a neuropeptide, ManducaFLRFamide, on the midgut activity of the sphingid moth, Agrius convolvuli.

The effect of ManducaFLRFamide, a neuropeptide originally isolated from the central nervous system of a sphingid moth, Manduca sexta, was examined on the midgut motility of another sphingid moth, Agrius convolvuli. The peptide was shown to suppress the spontaneous contractions of the isolated gut in adult Agrius at low concentrations (> or = 10(-10) M). Immunoreactivity against anti-FMRFamide antiserum was also detected in the brain-suboesophageal ganglion and the retrocerebral neurohaemal organs of the moth. These observations suggest that ManducaFLRFamide, or its closely-related analog, is a neuropeptide which regulates the activity of the digestive tract in Agrius.

Amino Acid Sequence↗

The moth as an allusion to (symbol of?) mother.

The meanings of the image of the moth are examined. The use of the moth as both victim and predator, with allusive and symbolic reference to parent and child, is elucidated. My emphasis is on the equation of the moth by children with their intrapsychic registration of a destructive yet vulnerable parent (usually mother) whom the child both wants to destroy and feels it cannot live without. This simple thesis is made use of chiefly to explicate aspects of the life and works of the great American writer, Elizabeth Bishop.

Animals↗

Cold hardiness adaptations of codling moth, cydia pomonella

The cold hardiness adaptations of natural and laboratory reared populations of the codling moth, Cydia pomonella, were examined. Hemolymph, gut, and whole body supercooling points (SCPs), 24-h LT50s, polyhydroxy alcohol concentrations, hemolymph freezing points, and hemolymph melting points were determined. Nondiapausing codling moth larvae do not have appreciable levels of ice nucleators in the hemolymph or gut. Whole body supercooling points were higher than hemolymph supercooling points. For nondiapausing larvae, LT50s were significantly higher than both the whole body and the hemolymph supercooling points, indicating the presence of chill sensitivity. As the larvae left the food source and spun a cocoon, both hemolymph and whole body SCPs decreased. Diapause destined larvae had significantly lower hemolymph SCPs than nondiapausing larvae, but whole body SCPs were not significantly different from nondiapausing larvae of the same age. The LT50s of diapause destined and diapausing larvae were significantly lower than that of nondiapausing larvae. Codling moths are freezing intolerant, with LT50s close to the average whole body supercooling point in diapause destined and diapausing larvae. The overwintering, diapausing larvae effectively supercool to avoid lethal freezing by removal of ice nucleators from the gut and body without appreciable increase of antifreeze agents such as polyols or antifreeze proteins.

Journal Article↗

Mitochondrial DNA variation among worldwide populations of gypsy moths, Lymantria dispar.

Gypsy moth populations from Japan, mainland Asia, Europe, Tunisia, and North America were analyzed for variation in mitochondrial DNA (mtDNA) sequences from three gene regions. These samples resolve into four groups, representing gypsy moths from (1) Okinawa, Japan, (2) Hokkaido, Japan, (3) Honshu and Kyushu, Japan and mainland Asia, and (4) Europe, Tunisia, and North America. Some patterns of geographic variation observed for mtDNA (for example, the distinctiveness of gypsy moths from Hokkaido, Japan) coincide with those observed by Goldschmidt from analyses of morphology, life history, and intersexuality. Other patterns (relative sequence homogeneity across Asia, Honshu, and Kyushu and reduced levels of variation in mainland Japan) do not.

Animals↗

The metathoracic wing-hinge chordotonal organ of an atympanate moth, Actias luna (Lepidoptera, Saturniidae): a light- and electron-microscopic study.

The structure of a simple chordotonal organ, the presumed homologue of the noctuoid moth tympanal organ, is described in the atympanate moth, Actias luna. The organ consists of a proximal scolopidial region and a distal strand, which attaches peripherally to the membraneous cuticle ventral to the hindwing alula. The strand is composed of elongate, microtubule-rich cells encased in an extracellular connective tissue sheath. The scolopidial region houses three mononematic, monodynal scolopidia, each comprised of a sensory cell, scolopale cell, and attachment cell. The dendritic apex is octagonally shaped in transverse section, its inner membrane lined by a laminated structure reminiscent of the noctuoid tympanal organ 'collar'. A 9 + 0-type cilium emerges from the dendritic apex, passes through both the scolopale lumen and cap, and terminates in an extracellular space distal to the latter. Proximal extensions of the attachment cell and distal prolongations of the scolopale cell surrounding the cap are joined by an elaborate desmosome, with which is associated an extensive electron-dense fibrillar plaque. Within the scolopale cell, this plaque constitutes the scolopale 'rod' material. The data are discussed in terms of both the organ's potential function, and its significance as the evolutionary prototype of the noctuoid moth ear.

Animals↗