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PubMed2005

Effect of extrusion of corn and feed form on feed quality and growth performance of poultry in a tropical environment.

Two experiments were conducted to study the effect of corn extrusion and feed form on feed quality and growth performance of broilers. In experiment 1, whole corn was extruded at a water flow rate of 16, 19, and 22 L/h. The experimental design was a completely randomized design with 3 replications for each treatment. The extrusion process significantly decreased (P < 0.01) ether extract, crude fiber, and moisture content of corn. Increased water flow significantly decreased enzyme susceptibility from 56 to 47% (P < 0.01), but increased the bulk density of extrudate from 134 to 224 g/L (P < 0.01). However, there was a trend for lower total energy consumption (P < 0.01) and extruding production rate (P < 0.05) when corn was processed at the water flow of 22 L/h. In Exp. 2, 1,600 broilers (initially BW of 41 g) were used to determine the effect of extrudate and feed form on the growth performance in poultry during a 42-d growth assay. There were 50 chicks per pen and 8 pens per treatment. Treatments were arranged in a 2 x 2 factorial arrangement with the main effect of feed form and the main effect of 25% processed corn inclusion. Treatments were 1) conventional corn mash, 2) conventional corn pelleted feed, 3) +25% extruded corn mash, and 4) +25% extruded corn pelleted feed. Chicks fed pelleted feed, regardless of processing of corn, had a greater BW and feed conversion than those fed mash feed diet (P < 0.001). Extruded corn mash decreased the growth performance. Results suggested that optimal growth rate and feed conversion of birds raised in a tropical environment given extruded corn might be achieved by steam pelleting.

Animal Feed↗

PubMed2005

Predicting effective caloric value of nonnutritive factors: III. Feed form affects broiler performance by modifying behavior patterns.

Two trials of an experiment were conducted to confirm the relationships among effective caloric value (ECV) of the diet, net energy for gain (NEg), BW, feed conversion ratio (FCR), and broiler behavior. Further, we sought to examine such factors with benefits of pelleting, including feed form history (pellets vs. mash) in females from 2 strains of commercial broilers. Composition of gain was measured on a sample of birds in both trials. In trial 1 birds were reared to 23 d on feed in crumble form, when the birds were provided a feed in pellet or mash form for 7 d. Pelleting the feed increased ECV and total NEg, while decreasing eating and increasing resting behavior. Significant correlations (P < 0.05) among resting, NEg, and ECV occurred. In trial 2, birds were reared to 23 d on a crumble diet and then fed diet in mash or pellet form to 36 d. At 37 d of age, half of the birds from each strain and feed form history combination were switched to the alternative feed form. Interactions of strain by grower feed form were present for BW, initial fat, and body energy content indicating that pelleted feed was required for optimum broiler performance of 1 strain. Grower feed form by finisher feed form interactions were present and demonstrated that birds switched from pellets or mash to the alternate feed form consumed more feed in less time than birds that remained on their previous feed form. Significant correlations were observed in both trials between behaviors and FCR and ECV, whereas NEg reflected these differences in trial 1 but not trial 2. Regression analysis indicated that FCR and subsequently ECV were best predicted by lean gain, whereas NEg was best predicted by fat gain. Further, regression analysis established interactive equations in which ECV was predicted (R2 > 0.99) by eating and resting behavior. The results of these trials indicate that the effects of feed form are caused by a modification of behavior patterns, that ECV is responsive to such behavior changes, and that ECV is an effective estimator of the relative caloric value of genetic, management, and husbandry influences.

Adipose Tissue↗

PubMed2006

Effects of particle size and physical form of diets on mast cell numbers, histamine, and stem cell factor concentration in the small intestine of broiler chickens.

The aim of this study was to investigate the hypothesis that particle size and diet form may affect the growth of mast cells and histamine release from the small intestine of broiler chickens. A total of 288, day-old male broiler chicks were randomly allocated to 1 of 4 corn-soy diets in a 2 x 2 factorial design. The factors included particle size (coarse vs. fine) and physical form (mash vs. pellet). The birds were housed in 90 x 60 cm pens containing 12 birds, and each treatment contained 6 replicate pens of birds from d 1 to 22. On d 22, 6 broilers from each treatment were slaughtered. Tissues from the small intestine (duodenum, jejunum, and ileum) were obtained to quantify mast cells using the toluidine blue staining technique. The results showed that mast cells in the jejunum were concentrated in the upper part of the villus in birds fed the coarsely ground mash diet, whereas mast cells were evenly distributed throughout the intestine in birds fed the other 3 diets. The number of mast cells was significantly lower in the duodenum (P = 0.04), jejunum (P < 0.01), and ileum (P = 0.01) of birds fed coarsely ground diets compared with finely ground diets, and there was no difference in mast cell numbers between birds fed mashed or pelleted diets at any site in the intestine. The histamine content (P = 0.02) and stem cell factor concentration (P = 0.03) were markedly lower in the jejunum of birds that were fed coarsely ground diets compared with finely ground diets. The stem cell factor concentration in the duodenum (P < 0.01) and jejunum (P = 0.05) was higher in birds fed pelleted compared with mash diets. The overall results of this experiment suggest that particle size and diet form affect mast cell number and histamine content in the small intestine by regulation of stem cell factor concentration.

Animal Feed↗

PubMed2000

Urea hydrogen peroxide reduces the numbers of lactobacilli, nourishes yeast, and leaves no residues in the ethanol fermentation.

Urea hydrogen peroxide (UHP) at a concentration of 30 to 32 mmol/liter reduced the numbers of five Lactobacillus spp. (Lactobacillus plantarum, L. paracasei, Lactobacillus sp. strain 3, L. rhamnosus, and L. fermentum) from approximately 10(7) to approximately 10(2) CFU/ml in a 2-h preincubation at 30 degrees C of normal-gravity wheat mash at approximately 21 g of dissolved solids per ml containing normal levels of suspended grain particles. Fermentation was completed 36 h after inoculation of Saccharomyces cerevisiae in the presence of UHP, even when wheat mash was deliberately contaminated (infected) with L. paracasei at approximately 10(7) CFU/ml. There were no significant differences in the maximum ethanol produced between treatments when urea hydrogen peroxide was used to kill the bacteria and controls (in which no bacteria were added). However, the presence of L. paracasei at approximately 10(7) CFU/ml without added agent resulted in a 5.84% reduction in the maximum ethanol produced compared to the control. The bactericidal activity of UHP is greatly affected by the presence of particulate matter. In fact, only 2 mmol of urea hydrogen peroxide per liter was required for disinfection when mashes had little or no particulate matter present. No significant differences were observed in the decomposition of hydrogen peroxide in normal-gravity wheat mash at 30 degrees C whether the bactericidal agent was added as H(2)O(2) or as urea hydrogen peroxide. NADH peroxidase activity (involved in degrading H(2)O(2)) increased significantly (P = 0.05) in the presence of 0.75 mM hydrogen peroxide (sublethal level) in all five strains of lactobacilli tested but did not persist in cells regrown in the absence of H(2)O(2). H(2)O(2)-resistant mutants were not expected or found when lethal levels of H(2)O(2) or UHP were used. Contaminating lactobacilli can be effectively managed by UHP, a compound which when used at ca. 30 mmol/liter happens to provide near-optimum levels of assimilable nitrogen and oxygen that aid in vigorous fermentation performance by yeast.

Carbamide Peroxide↗

PubMed1992

Monoclonal antibody solution hybridization assay for detection of mouse hepatitis virus infection.

A monoclonal antibody solution hybridization (MASH) assay was developed to detect fecal excretion of mouse hepatitis virus (MHV). The assay used a biotinylated cDNA probe to detect viral RNA target sequences by hybridization in solution, capture of hybrids on the solid phase with antibiotin antibody, and immunoassay with an enzyme-labelled monoclonal antibody specific for DNA-RNA hybrids. The MASH assay was used to monitor the time course of enterotropic MHV excretion after oronasal inoculation. Infectivity of the inoculated mice was simultaneously monitored with sentinel animals. The MASH assay detected MHV excretion in all inoculated mice, with the highest mean excretion levels occurring from day 3 through day 9 postinoculation. Mean excretion then decreased gradually to below detection limits by day 21 postinoculation. Sentinels became infected on exposure to inoculated mice up to but not after day 21 postinoculation. Infected sentinel mice showed a time course of virus excretion similar to that of inoculated mice. These results indicate that the MASH assay is useful for rapid, sensitive, and specific detection of MHV in clinical specimens from laboratory mice.

Animals↗

PubMed2004

Overexpression of lunatic fringe does not affect epithelial cell differentiation in the developing mouse lung.

The Notch/Notch-ligand pathway regulates cell fate decisions and patterning in various tissues. Several of its components are expressed in the developing lung, suggesting that this pathway is important for airway cellular patterning. Fringe proteins, which modulate Notch signaling, are crucial for defining morphogenic borders in several organs. Their role in controlling cellular differentiation along anterior-posterior axis of the airways is unknown. Herein, we report the temporal-spatial expression patterns of Lunatic fringe (Lfng) and Notch-regulated basic helix-loop-helix factors, Hes1 and Mash-1, during murine lung development. Lfng was only expressed during early development in epithelial cells lining the larger airways. Those epithelial cells also expressed Hes1, but at later gestation Hes1 expression was confined to epithelium lining the terminal bronchioles. Mash-1 displayed a very characteristic expression pattern. It followed neural crest migration in the early lung, whereas at later stages Mash-1 was expressed in lung neuroendocrine cells. To clarify whether Lfng influences airway cell differentiation, Lfng was overexpressed in distal epithelial cells of the developing mouse lung. Overexpression of Lfng did not affect spatial or temporal expression of Hes1 and Mash-1. Neuroendocrine CGRP and protein gene product 9.5 expression was not altered by Lfng overexpression. Expression of proximal ciliated (beta-tubulin IV), nonciliated (CCSP), and distal epithelial cell (SP-C, T1alpha) markers also was not influenced by Lfng excess. Overexpression of Lfng had no effect on mesenchymal cell marker (alpha-sma, vWF, PECAM-1) expression. Collectively, the data suggest that Lunatic fringe does not play a significant role in determining cell fate in fetal airway epithelium.

Animals↗

PubMed2006

Characteristics and modeling of Escherichia coli growth in pouched food.

Characteristics of the growth kinetics of Escherichia coli cells in pouched mashed potatoes under various conditions were studied with a mathematical model. Bacterial cells were inoculated in sterile mashed potatoes and then sealed in vinyl pouches, in which a very small amount of air was included. The growth curves of cells in the pouched mashed potatoes at constant temperature (12-34 degrees C) were sigmoidal with time on a semi-logarithmic plot and were successfully described with a new logistic model recently developed by us. The rate constant of growth showed a highly linear relationship to the temperature with the square-root model, and the lag period was longer at lower temperatures. The growth curve in glass tubes containing a large volume of air was similar to that in pouches, showing that the rate of growth was not affected by the volume of the surrounding air. The growth curves in pouched mashed potatoes were very similar to those in nutrient broth or on the surface of nutrient agar, which we previously reported. These results suggested that the growth kinetics of the bacterial cells under various conditions of rich nutrition might be almost identical, and can be described with a simple growth model like ours.

Escherichia coli↗

PubMed1986

Egg quality and hen performance responses to protein-calcium deficiency, cafeteria feeding, and cage density.

Single Comb White Leghorn (SCWL) hens were given feeds (2975 kcal ME/kg) that were marginally deficient in protein (15%) and calcium (3.0%). Negative control was as a mash, whereas an experimental mixture had the same formulation, but 5 of the 15% protein was soybean pellets in place of the meal and 2 of the 3% calcium was oyster shell flakes. A complete cafeteria system acted as a positive control and provided pellets, oyster shell, and a 10% protein-1% calcium mash (2975 kcal ME/kg) separately in a three-compartment trough. Feed treatments were imposed from 24 to 40 weeks of age on hens kept one and two/cage. Performance effects, because of the feed treatments, were not apparent until after 8 weeks of experimentation. Production was sustained by the pellet-oyster shell feed mixture when birds given the complete mash declined. Feeds had immediate effects on egg quality. Egg shell deformation and albumen Haugh values were highest with the complete mash and lowest for the cafeteria system, while the complete mixture was generally between these extremes. Hens kept two/cage consumed more feed than those one/cage and responded similarly to each treatment, but the reduced performance associated with increased density was not alleviated.

Albumins↗

PubMed2004

[Reduction of reconstruction time and data volume of sinogram by angular compression for clinical three-dimensional whole body FDG-PET].

In positron emission tomography (PET), the large number of lines of responses in three-dimensional (3D) acquisition mode creates a high volume of sinogram data and increases reconstruction time in iterative reconstruction. We tried to decrease sinogram data volume by reducing the number of views using angular compression and then evaluated the accuracy of this mashed mode. Three methods were compared, conventional mode (CONV), X2 mashed mode (X2: two adjacent projection angles are added together), and X4 mashed mode (X4: four adjacent angles added). A point source of (18)F was used to measure spatial resolution. A hot spot phantom made of 6 hot spheres (10-38 mm in diameter) within water of 20 cm in diameter was scanned to evaluate the recovery coefficient (RC). A lung-heart-liver phantom made of homogeneous radioactive myocardium, a spherical hot mass in the lung (10 mm in diameter), and background activity in the liver was scanned to evaluate the homogeneity of the myocardial wall. The quality of the reconstructed images was evaluated in terms of the normalized mean square error (NMSE), Bull's eye map, profile curve, and peak value of the spherical hot mass. The reconstruction times of X2 and X4 were one-half and one-quarter, respectively, of that of CONV. In terms of spatial resolution, FWHM of CONV, X2, and X4 were, 4.26, 4.33, and 4.48 (mm) at the center, 4.81, 5.68, and 8.73 tangentially, and 8.01, 8.19, and 8.27 radially at R=200 mm, respectively. RC was similar for all methods. The NMSE values of X2 and X4 compared with CONV were 0.0003 and 0.0014, respectively. In the hot mass, these methods showed almost the same profile curves, although the peak value of X4 was only -1.95% less than that of CONV. Although the result of spatial resolution of X4 was slightly degraded, image quality and physical performance were good. Therefore, the X4 mashed mode used with angular compression was considered clinically useful.

Humans↗

PubMed1991

[Effects of sodium chloride on destruction of microorganisms by microwave heating in potatoes].

To assess the destructive effect of different sodium chloride concentrations (0, 0.3, 1.5, and 3%) on microorganisms with microwave heating, strains of Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella enteritidis, Escherichia coli and Bacillus cereus were used to inoculate a mashed potato preparation. After microwave heating for 1 min at 800 W, resulted in temperatures and rate of destruction of bacteria significantly higher (p less than 0.05) in the core than on the surface of mashed potato when no salt was added. Except for B. cereus, microorganisms inoculated into mashed potato, with no added salt, could be completely destroyed by 2 min of microwave heating. Core temperatures and rate of destruction of microorganisms in the mashed potato decreased significantly (p less than 0.05) with increase in concentration of added salt. The results also indicate that bacterial species differ in their susceptibility to microwave inactivation. In particular, S. aureus, which exhibited a level of survival that was greater than that of any other species tested here, seemed notably resistant to microwave treatment for 2 min while V. parahaemolyticus was highly susceptible.

Bacteria↗

PubMed1986

Aetiology of food-related oral lesions in chickens.

Brown Leghorn chickens fed on a mash diet developed ulcerated oral lesions with extensive epithelial erosion and large colonies of bacteria. Some birds had lesions by six weeks old and by 30 weeks oral lesions were present in all birds fed on a mash diet. These lesions occur infrequently in birds fed a pelleted diet and the relatively extensive lesions shown by birds on a mash diet heal quickly (in many cases within two weeks) when the birds are transferred to a pelleted diet. The cause of the lesion is unknown but there was no evidence for food impaction, mechanical damage to the epithelium, specific dietary constituents or blocked salivary ducts. The lesions may be due to poor oral hygiene which results from the lack of mechanical stimulation of the oral epithelium to which fine particles of mash adhere.

Animal Feed↗

PubMed1984

Effect of different starchy foods in composite meals on gastric emptying rate and glucose metabolism. I. Comparisons between potatoes, rice and white beans.

Thirteen healthy subjects participated in the study. Three meals of equal energy content consisting of hamburgers and mashed potatoes, polished rice or white beans were studied. The various meals were labelled with 2.6 MBq 51Cr and gastric emptying was studied by a movable NaI (T1) detector. Blood glucose and plasma insulin were measured after an overnight fast and after ingestion of the testmeal. Gastric emptying of mashed potatoes was faster than that of polished rice (P less than 0.02) and of white beans (P less than 0.05). Blood glucose and plasma insulin responses to the meal with mashed potatoes were greater than to that with polished rice (P less than 0.01), which also produced a greater glucose response than that with white beans (P less than 0.01). The correlation coefficient (rs) between difference in gastric emptying and difference in blood glucose or plasma insulin response was 0.95 (P less than 0.01) when comparing meals of mashed potatoes and polished rice. No correlation was found between gastric emptying and blood glucose or plasma insulin after the white bean meal. We conclude that glucose and insulin responses to potatoes and rice are strongly related to gastric emptying rate, but other factors dominate the control of these responses to white beans.

Adult↗

PubMed2026

Clinical Trial: Phase 3 Trial of Resmetirom Versus Placebo in Metabolic Dysfunction-Associated Steatohepatitis-Reanalysis of Fibrosis Stage 2-3 Subset.

BACKGROUND: Resmetirom is an oral thyroid hormone receptor beta agonist clinically used to treat metabolic dysfunction-associated steatohepatitis (MASH) among adults with stage F2 or F3 fibrosis. AIMS: Because the pivotal, 52-week, randomized, controlled, phase 3 MAESTRO-NASH trial (once-daily oral resmetirom 80 or 100 mg or placebo) included patients with F1, F2, or F3 fibrosis, we conducted a post hoc analysis aimed at assessing treatment response in the subset of patients with stages F2 and F3 fibrosis, consistent with the approved label population. METHODS: Co-primary end points were MASH resolution (hepatocellular ballooning score 0, lobular inflammation score ≤ 1, and ≥ 2-point nonalcoholic fatty liver disease activity score [NAS] reduction from baseline) with no fibrosis worsening, and ≥ 1-stage fibrosis improvement with no NAS worsening at Week 52. RESULTS: Among 917 patients with F2 or F3 fibrosis, metabolic risk factor prevalence was high (hypertension, 78.0%; dyslipidemia, 71.1%; type 2 diabetes, 67.0%). MASH resolution was achieved by 25.7% in the 80-mg group, 29.9% in the 100-mg group, and 9.5% in the placebo group (p < 0.0001 for both comparisons with placebo). Respective percentages with fibrosis improvement were 26.5%, 28.9%, and 17.3% (p < 0.01 for both comparisons). From baseline to Week 24, low-density lipoprotein cholesterol decreased by 11.7% and 13.7% in the 80- and 100-mg resmetirom groups, respectively, and increased by 2.3% with placebo (p < 0.0001 for both comparisons). No new safety signals emerged. CONCLUSION: Results among patients with F2 and F3 fibrosis were consistent with the primary MAESTRO-NASH analysis population, demonstrating efficacy and safety of resmetirom after 52 weeks.

Humans↗

PubMed1998

Development of LBP110 expression by neural crest-derived enteric precursors: migration and differentiation potential in ls/ls mutant mice.

Neural crest-derived cells acquire a 110-kD laminin-binding protein (LBP110) when they colonize the murine bowel. Laminin stimulates LBP110-expressing cells to develop as neurons. We have followed the development of LBP110 by neural crest-derived cells as they enter the gut of control and ls/ls mutant mice. The expression of neurofilament and choline acetyltransferase was used as markers of a neuronal phenotype. Tyrosine hydroxylase was used as a marker for the mash-1-dependent lineage of enteric precursors, while calcitonin gene-related peptide was used as a marker for the mash-1-independent lineage of crest-derived cells. A subset of cells expressing LBP110 was located along the vagi at E10 at cervical and thoracic levels. At E12, cells expressing LBP110 extended from the foregut to the midgut. The expression of neurofilament protein lagged behind that of LBP110 by about 0.5 day and then became coincident with LBP110 immunoreactivity. By E15, cells doubly labeled with antibodies to LBP110 and neurofilament protein were located along the entire extent of the bowel up to but not including the terminal colon. By E16, both the proximal and terminal colon contained cells expressing LBP110 and neurofilaments. The pattern of immunoreactivity could not be distinguished between ls/ls and control animals prior to E16. By E16, when the terminal colon of control animals contained many cells expressing LBP110 and neurofilaments, the terminal colon of ls/ls animals lacked cells expressing these proteins; nevertheless, structures outside of the terminal colon were heavily endowed with cells expressing LBP110 and neurofilaments. These ectopically located cells derived from both mash-1-dependent and -independent lineages of crest-derived precursors.

Animals↗

PubMed1993

Prior exposure to low or high fat milk enhances naloxone anorexia in rats.

Opioid peptides have been implicated in reward-related components of eating, especially with fatty and sweetened foods. This study examined the effect of pre-exposure of rats to milk on the subsequent anorexic effect of naloxone in a separate daily mash intake test. Rats were given constant access to either skimmed or whole milk for 15 days, in addition to normal diet. A third (control) group received only the normal diet. Subsequently, all groups received 0, 0.125, 0.5 and 1.0 mg/kg naloxone prior to their daily mash intake test. Naloxone caused dose-dependent reductions in mash intake in all groups, but this effect was significantly greater in the group with whole milk than in controls, with the group with skimmed milk intermediate. Daily intakes of milk were similar with skimmed and whole milk, and milk intake in separate two-bottle choice tests provided no evidence for overall preferences for either solution. These results suggest that prior exposure to milk enhances opioid involvement in feeding, and possible mechanisms for this are discussed.

Animals↗

PubMed2000

Midline nasal tissue influences nestin expression in nasal-placode-derived luteinizing hormone-releasing hormone neurons during development.

Neurons differentiating into the luteinizing hormone-releasing hormone (LHRH) neuroendocrine phenotype are derived from the nasal placode. Cells within the vomeronasal organ anlage that turn on LHRH gene and peptide expression subsequently migrate into the forebrain where they influence reproductive function. The molecular and cellular cues regulating differentiation and migration of these cells are unknown. Discovery of developmental markers can indicate proteins directing or associated with differentiation. Analysis of such markers after manipulation of external cues can elucidate important extracellular differentiation signals. Embryonic LHRH neurons were examined in vivo for Mash-1 and nestin, two factors that delineate precursor populations in PNS and forebrain CNS cells. Nestin, but not Mash-1, was detected in early expressing LHRH cells in the vomeronasal organ anlage. These results were duplicated in LHRH neurons maintained in vitro in nasal explants. Such LHRH cells expressed nestin mRNA but not Mash-1 mRNA and were also negative for three other olfactory epithelial developmental transcription factors, Math4A, Math4C/neurogenin1, and NeuroD mRNA. Experimental manipulation of nasal explants revealed dual expression of nestin protein and LHRH in cells proximal to the vomeronasal organ anlage that was dependent upon midline cartilaginous/mesenchymal tissues. Prolonged nestin expression in LHRH cells after midline removal is consistent with nasal midline tissues modulating differentiation of LHRH neurons from the nasal placode.

Animals↗

PubMed1992

The effect of dietary sodium fluoride on internal organs, breast muscle, and bones in captive American kestrels (Falco sparverius).

In 1982, 29 7-day-old American kestrel (Falco sparverius) chicks from captive stock were randomly assigned to one of three dietary regimens: (1) 10 birds were fed daily with cockerel mash (0 ppm of F-: control birds); (2) 10 birds were fed daily with cockerel mash containing 1,120 ppm of F-; (3) 9 birds were fed daily with cockerel mash containing 2,240 ppm of F-. Growth of the kestrels was not significantly affected by NaF in their diet. No significant differences were found among the 3 groups for length of duodenum, jejunum and ileum. Rectum was longer as more fluoride was added to the diet. Weights of adrenals, brain, gizzard, spleen, heart, kidneys, liver, pancreas, and pectoral muscle were not significantly affected by treatment, although kidneys, spleen and adrenals tended to become lighter. Percent bone ash was significantly (P less than 0.05) increased, while bone breaking strength was significantly (P less than 0.05) decreased by treatment.

Animals↗

PubMed1996

Behavioural evidence that d-fenfluramine-induced anorexia in the rat is not mediated by the 5-HT1A receptor subtype.

These studies investigated the involvement of the 5-HT1A receptor in mediating d-fenfluramine-induced anorexia in the rat. Non-deprived, d-fenfluramine-treated (3.0 mg/kg) rats consumed a reduced amount of a palatable wet mash and showed a temporal advance in the behavioural sequence consistent with satiety. Thus, rats treated with d-fenfluramine ceased feeding and began resting before corresponding controls. Pretreatment with the selective 5-HT1A receptor antagonist WAY-100,635 (1.0 mg/kg) had no effect on either the reduced mash consumption or behavioural satiety sequence of d-fenfluramine-treated animals at a dose which was found to attenuate the anorexia induced by the 5-HT1A receptor agonist 8-OH-DPAT (0.5 mg/kg). Pretreatment with the non-selective 5-HT antagonist metergoline (1.0 mg/kg) attenuated the d-fenfluramine-induced reduction of mash consumption and the advanced offset of feeding. Metergoline pretreatment had no effect on the advanced onset of resting observed in d-fenfluramine-treated animals. These data suggest that d-fenfluramine reduces food intake, perhaps by enhancing satiety, via a mechanism which does not involve the 5-HT1A receptor subtype. The implications of these results to the utility of the behavioural satiety sequence as a measure of postprandial satiety are discussed.

8-Hydroxy-2-(di-n-propylamino)tetralin↗