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Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems.

Samples from 2427 cattle, 661 goats, 104 sheep, 98 camels and 82 horses were screened for brucella infections by the Rose Bengal Test and positive reactors confirmed by the complement fixation test. In cattle, the highest individual seroprevalence was in dairy herds kept under the intensive husbandry system, with an individual prevalence of 8.2% and unit (herd) seroprevalence of 35.9%. This was followed by the pastoral husbandry system in the Western Lowlands with 5.0% individual but a higher unit (vaccination site) prevalence of 46.1%. The lowest was in the mixed crop-livestock system in the Southern Highlands with individual 0.3% and unit (village) prevalence of 2.4%. In sheep and goats, no positive animals were detected in the mixed crop-livestock areas. In the Eastern Lowlands individual prevalences of 3.8% (goats) and 1.4% (sheep) and unit prevalence of 33.3% (goats) and 16.7% were found, while 14.3% of individual goats and 56.3% of the units in the Western Lowlands were positive. No positive horses were found. The present study documents the first serological evidence of Brucella spp. infection in camels (3.1%) in Eritrea.

Animal Husbandry↗

Genetic legacy effects in a mungbean-wheat rotation reveal potential to breed for system-level yield gains.

Legume crops provide protein-rich food, serve as critical disease breaks in cereal rotations, and contribute to soil fertility through symbiotic nitrogen fixation. However, crop improvement programs typically focus on within-crop performance rather than system-level benefits. We hypothesize that legacy effects (the influence of one crop's genotype on subsequent crop performance) are under genetic control and could be targeted in breeding programs. To test this, we evaluated how 309 genetically diverse mungbean genotypes influenced subsequent wheat performance. The mungbean panel was grown, followed by a single wheat cultivar sown in the same plots. Remarkably, wheat yield varied by nearly 1 t ha-1 (2.52-3.49 t ha-1), depending solely on the preceding mungbean genotype. Legacy effects showed moderate heritability (H2: 0.43-0.65), suggesting untapped genetic potential for breeding. However, these estimates were derived from a single site and season and require validation across environments. Analyses of mungbean traits, soil properties, and volatile organic compounds identified root architecture, symbiotic nitrogen fixation, and the soil microbiome as potential contributors to legacy effects, although these mechanisms remain to be tested directly. Haplotype mapping identified genomic regions in mungbean associated with wheat yield and, to a lesser extent, grain protein, revealing trade-offs between within-crop performance and legacy effects. Genetic simulations based on empirically derived marker effects compared genomic selection strategies targeting mungbean yield, wheat yield, or both simultaneously. A selection strategy placing equal weight on mungbean yield and subsequent wheat yield (50:50 weighting) achieved simultaneous gains in both crops (19.5% and 7.6%), highlighting the potential to breed for system-level productivity with reduced input requirements.

crop rotations↗

Suggestions for crops grown in controlled ecological life-support systems, based on attractive vegetarian diets.

Assuming that crops grown in controlled ecological life-support systems (CELSS) should provide a basis for meals that are both nutritious and attractive (to taste and vision), and that CELSS diets on the moon or Mars or in space-craft during long voyages will have to be mostly vegetarian, a workshop was convened at the Johnson Space Center, Houston, Texas, U.S.A. on 19 to 21 January, 1994. Participants consisted of trained nutritionists and others; many of the approximately 18 presenters who discussed possible diets were practicing vegetarians, some for more than two decades. Considering all the presentations, seven conclusions (or points for discussion) could be formulated: nutritious vegetarian diets are relatively easily to formulate, vegetarian diets are healthy, variety is essential in vegetarian diets, some experiences (e.g., Bios-3 and Biosphere 2) are relevant to planning of CELSS diets, physical constraints will limit the choice of crops, a preliminary list of recommended crops can be formulated, and this line of research has some potential practical spinoffs. The list of crops and the reasons for including specific crops might be of interest to professionals in the field of health and nutrition as well as to those who are designing closed ecological systems.

Crops, Agricultural↗

Modelling for precision weed management.

Recently, the need for the development of weed management systems with a reduced dependency on herbicides has increased because of concern about environmental side-effects and cost. The development of such systems requires new strategies based on improvements with respect to (1) prevention, (2) decision making and (3) weed control technology. For the development of improvements in all three aspects, quantitative understanding of weed population dynamics and crop-weed interactions is needed. Models that integrate the available quantitative knowledge can be used to design preventive measures, to develop long-term and short-term strategies for weed management, to assist in decision making to determine if, when, where and how weeds should be controlled and to identify new opportunities for weed control. Ecophysiological simulation models for crop-weed competition simulate growth and production of species in mixtures, based on ecophysiological processes in plants and their response to the environment. Such models help improve insight into the crop-weed system and can be used for purposes such as the development of simple predictive yield-loss models, threshold levels or the design of competitive crop plant types. For strategic weed management decisions, preventive measures and the identification of new opportunities for weed control, quantitative insight into the dynamics and spatial patterns of weed populations is also required. The complexity of the process and the long-term character of weed population dynamics make the use of models necessary. Different modelling approaches have been developed and are described briefly. Opportunities to use the available knowledge and models to improve weed management are discussed. Weeds occur in patches and their sensitivity to herbicides changes strongly with developmental stage, making precision techniques for herbicide application in time and space an option for reducing herbicide use. Limitations related to insight in biological processes as well as the state of technological development are discussed.

Agriculture↗

Description of the life-cycle of the pearl millet rust fungus--Puccinia substriata var. penicillariae with a proposal of reducing var. indica to a synonym.

Pearl millet (Pennisetum glaucum) is a major staple food crop in the drier parts of the old world, like Africa and India. Recently, its cultivation became more widespread in no-tillage crop farming systems in central Brazil, but it is also being used for cultivation during the dry season in other areas in Brazil. An emerging problem for the wider adoption of this crop in Brazil is the damage caused by the rust Puccinia substriata. This fungal disease is among the worst limitations for this crop worldwide. The rust occurring in Brazil was initially identified as the P. substriata var. penicillariae, but little information on the taxonomy and life-cycle of this rust is available in Brazil. The life-cycle of this rust variety remains somewhat obscure and the connection between the telial stage on pearl millet and the aecial stage on Solanaceae has never been experimentally demonstrated. Natural infection and inoculations under controlled conditions allowed for a complete description of all stages of this rust and the elucidation of its life-cycle, confirming that Solanum aethiopicum and Solanum melongena are aecial hosts. This coincidence for the alternate host and the limited and ambiguous morphological basis for the distinction of the varieties penicillariae and indica support the view that they are synonyms. Var. indica should be regarded as a late synonym of var. penicillariae.

Basidiomycota↗

Behavior of 14C-atrazine in Argentinean topsoils under different cropping managements.

Atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine) behavior was studied in four surface soils during incubations in laboratory conditions. Soils were chosen in relation to their cropping management (tillage and no tillage) and crop rotation system (continuous soybean [Glycine mar (L.) Merr.] and maize (Zea mays L.)-soybean rotation). A natural soil under brushwood was sampled as a reference. Atrazine use in field conditions was associated with maize cropping, thus only one soil received atrazine every other year. Atrazine behavior was characterized through the balance of 14C-U-ring atrazine radioactivity among the mineralized fraction, the extractable fraction, and the nonextractable bound residues. Soil organic matter capacity to form bound residues was characterized using soil size fractionation. Accelerated atrazine mineralization was only observed in the soil receiving atrazine in field conditions. Atrazine application every other year was enough to develop a microflora adapted to triazine ring mineralization. Bound residue formation was rapid and increased with soil organic matter content. The coarsest soil size fractions (2000-200 and 200-50 microm) containing the nonhumified organic matter presented the highest capacity to form bound residues. No effect of tillage system was observed, probably because of the uniform sampling depth at 20 cm, hiding the stratification pattern of soil organic matter in non-tilled soils.

Agriculture↗

[Identification and determination of unknown chemicals in crops by dual-column GC system equipped with MS and ECD].

A dual-column GC system equipped with MS and ECD, using two identical columns (HP-1701), one connected to the MS and one to the ECD, has been developed. The carrier gas flow rate was adjusted to give the same retention time of each peak in both columns. Unknown peaks were detected and identified in 7 samples of crops using this system. It was confirmed that the unknown peaks were 5 pesticides, i.e., bromopropylate and others. The recoveries and CV of bromopropylate from spinach and Komatsuna were good. The results of determination of bromopropylate by both MS and ECD were the same. Unknown chemicals can be easily identified using GC equipped with MS and ECD, and this system should be useful in the field of food hygiene.

Crops, Agricultural↗

Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies.

This report describes the stable expression of a medically important antibody in the staple cereal crops rice and wheat. We successfully expressed a single-chain Fv antibody (ScFvT84.66) against carcinoembryonic antigen (CEA), a well characterized tumor-associated marker antigen. scFv constructs were engineered for recombinant antibody targeting to the plant cell apoplast and ER. Up to 30 microg/g of functional recombinant antibody was detected in the leaves and seeds of wheat and rice. We confirmed that transgenic dry seeds could be stored for at least five months at room temperature, without significant loss of the amount or activity of scFvT84.66. Our results represent the first transition from model plant expression systems, such as tobacco and Arabidopsis, to widely cultivated cereal crops, such as rice and wheat, for expression of an antibody molecule that has already shown efficacy in clinical applications. Thus, we have established that molecular pharming in cereals can be a viable production system for such high-value pharmaceutical macromolecules. Our findings provide a strong foundation for exploiting alternative uses of cereal crops both in industrialized and developing countries.

Animals↗

Ruminant nutrition from an environmental perspective: factors affecting whole-farm nutrient balance.

Nutrient budgeting strategies focus primarily on recycling manure to land as fertilizer for crop production. Critical elements for determining environmental balance and accountability require knowledge of nutrients excreted, potential nutrient removal by plants, acceptable losses of nutrients within the manure management and crop production systems, and alternatives that permit export of nutrients off-farm, if necessary. Nutrient excretions are closely related to nutrient intake and can be predicted by subtracting predicted nutrients in food animal products exported from the farm from total nutrients consumed. Intensifying crop production with double- or triple-cropping often is necessary for high-density food animal production units to use manure without being forced to export manure or fertilizer coproducts to other farms. Most manures are P-rich relative to N largely because of 1) relatively large losses of volatilized NH3, most of it converted from urea in urine, 2) denitrification losses in soil under wet, anaerobic conditions, and 3) ability of many crops to luxury-consume much more N than P. Most soils bind P effectively and P usually is permitted to accumulate, allowing for budgets to be based on N. However, P budgeting may be required in regions where surface runoff of P contributes to algae growth and eutrophication of surface waters or where soil P increases to levels of concern. Research is needed to determine whether dietary P allowances can be lowered without detriment to animal production or health in order to lower P intake and improve N:P ratios in manure relative to fertilization needs.

Animal Nutritional Physiological Phenomena↗

Plastic cup traps equipped with light-emitting diodes for monitoring adult Bemisia tabaci (Homoptera: Aleyrodidae).

Equipping the standard plastic cup trap, also known as the CC trap, with lime-green light-emitting diodes (LED-plastic cup trap) increased its efficacy for catching Bemisia tabaci by 100%. Few Eretmocerus eremicus Rose and Zolnerowich and Encarsia formosa Gahan were caught in LED-plastic cup traps. The LED-plastic cup traps are less expensive than yellow sticky card traps for monitoring adult whiteflies in greenhouse crop production systems and are more compatible with whitefly parasitoids releases for Bemisia nymph control.

Animals↗

Mitigation using a tandem construct containing a selectively unfit gene precludes establishment of Brassica napus transgenes in hybrids and backcrosses with weedy Brassica rapa.

Transgenic oilseed rape (Brassica napus) plants can interbreed with nearby weedy Brassica rapa, potentially enhancing the weediness and/or invasiveness of subsequent hybrid offspring. We have previously demonstrated that transgenic mitigation effectively reduces the fitness of the transgenic dwarf and herbicide-resistant B. napus volunteers. We now report the efficacy of such a tandem construct, including a primary herbicide-resistant gene and a dwarfing mitigator gene, to preclude the risks of gene establishment in the related weed B. rapa and its backcross progeny. The transgenically mitigated and non-transgenic B. rapa x B. napus interspecific hybrids and the backcrosses (BC(1)) with B. rapa were grown alone and in competition with B. rapa weed. The reproductive fitness of hybrid offspring progressively decreased with increased B. rapa genes in the offspring, illustrating the efficacy of the concept. The fitness of F(2) interspecific non-transgenic hybrids was between 50% and 80% of the competing weedy B. rapa, whereas the fitness of the comparable T(2) interspecific transgenic hybrids was never more than 2%. The reproductive fitness of the transgenic T(2) BC(1) mixed with B. rapa was further severely suppressed to 0.9% of that of the competing weed due to dwarfism. Clearly, the mitigation technology works efficiently in a rapeseed crop-weed system under biocontainment-controlled environments, but field studies should further validate its utility for minimizing the risks of gene flow.

Brassica napus↗

Mapping the potential distribution of Phlebotomus martini and P. orientalis (Diptera: Psychodidae), vectors of kala-azar in East Africa by use of geographic information systems.

The distribution of two principal vectors of kala-azar in East Africa, Phlebotomus martini and Phlebotomus orientalis were analysed using geographic information system (GIS) based on (1) earth observing satellite sensor data: Normalized Difference Vegetation Index (NDVI) and midday Land Surface Temperature (LST) derived from advanced very high resolution radiometer (AVHRR) of the global land 1km project of United States Geological Survey (USGS), (2) agroclimatic data from the FAO Crop Production System Zone (CPSZ) of the Intergovernmental Authority on Development (IGAD) sub-region, and (3) the FAO 1998 soils digital map for the IGAD sub-region. The aim was to produce a predictive risk model for the two vectors. Data used for the analysis were based on presence and absence of the two species from previous survey collections in the region (mainly Ethiopia, Kenya and Somalia). Annual, wet season and dry season models were constructed. Although all models resulted in more than 85% positive predictive values for both species, the best fit for the distribution of P. martini was the dry season composite (NDVI 0.07-0.38 and LST 22-33 degrees C) with a predictive value of 93.8%, and the best fit for P. orientalis was the wet season composite (NDVI -0.01 to 0.34 and LST 23-34 degrees C) with a predictive value of 96.3%. The two seasonal composites models derived from satellite data were largely similar with best fit models developed based on the CPSZ climate data: average altitude (12-1900m), average annual mean temperature (15-30 degrees C), annual rainfall (274-1212mm), average annual potential evapotranspiration (1264-1938mm) and readily available soil moisture (62-113mm) for P. martini; and average altitude (200-2200m), annual rainfall (180-1050mm), annual mean temperature (16-36 degrees C) and readily available soil moisture (67-108mm) for P. orientalis. Logistic regression analysis indicated LST dry season composite of the satellite data, average altitude, mean annual temperature and readily available soil moisture of the CPSZ data as the best ecological determinants for P. martini while LST annual composite was the only important ecological determinant for P. orientalis. Spearman's rank correlation revealed several factors to be important determinants for the distribution of the two vectors. None of the soil types analysed appeared to be important determinant for the two species in East Africa, unlike in Sudan where P. orientalis is mainly associated with eutric vertisol (black cotton clay soil).

Africa, Eastern↗

[Root growth characteristics of soybean and Faba bean at their seedling stage].

Soybean and faba bean are the two main legumes widely intercropped with cereals (e. g., wheat or maize) in western China, but have significantly different performance in intercropping system. Compared to soybean, faba bean has a stronger competitive ability. Considering that underground interspecific interaction makes a significant contribution to the advantage of intercropping system, a pot experiment was conducted to compare the differences in root growth and morphological parameters between soybean and faba bean at their seedling stage. A PVC tube, with 10 cm in diameter and 50 cm in depth, was filled with quartz and used to grow the crops. Root systems were scanned by a scanner, and analyzed by WinRHIZO on days 14, 21, 28, 35 and 42 after seedling emergence. The results indicated that on the 42nd day after seedling emergence, the root surface area of faba bean was 2.61 times larger than that of soybean. Soybean roots distributed in the whole layer of quartz on the 14th day, but most faba bean roots only distributed in its top layer. Soybean roots were thinner, and most of them were 0.25 approximately 1.0 mm in diameter, while faba bean roots were thicker, and their diameter was mostly between 0.5 approximately 1.5 mm. All of these facts resulted in a higher absorption capacity and nutrient use efficiency of faba bean, which could be one of the reasons that faba bean had a stronger competitive capacity than soybean.

Plant Roots↗

[Effects of inoculation with Rhizobium leguminosarum biovar trifolii on wheat cultivated in clover crop rotation agricultural soil in Morocco].

One hundred strains of Rhizobium leguminosarum bv. trifolii were isolated from roots of wheat cultivated in rotation with clover in two different regions of Morocco. The isolates were first screened for their effect on the growth of the cultivar Rihane of wheat cultivated in an agricultural soil under greenhouse conditions. After 5 weeks of growth, 14 strains stimulating the fresh or dry matter yield of shoots were selected and used in a second pot inoculation trial performed with two different agricultural soils. The results show that the strains behaved differently according to the soil used. In the loamy sand Rabat, strain IAT 168 behaved potentially like a plant growth promoting rhizobacteria (PGPR), as indicated by the 24% increases (P < 0.1) observed in wheat shoot dry matter and grain yields. In the silty clay Merchouch, no PGPR activity was observed, and 6 strains showed a significant deleterious effect on yields. These observations suggest that it is very important in a crop rotation system to choose a R. leguminosarum bv. trifolii strain that is effective with clover and shows PGPR activity with wheat to avoid deleterious effects on wheat yields.

Crops, Agricultural↗

Development of alternative strategies for management of soilborne pathogens currently controlled with methyl bromide.

The current standard treatment for management of soilborne pests in some high-value crop production systems is preplant fumigation with mixtures of methyl bromide and chloropicrin. With the impending phase-out of methyl bromide, the agricultural industries that rely on soil fumigation face the need for development of alternative pest management strategies. To maintain farm productivity, immediate term research has focused on evaluation of alternative fumigants, modification of current crop production practices to accommodate their use, and improvement of application technologies to reduce the environmental effects of fumigant applications. Longer-term research goals have focused on developing a more integrated approach for pest management that incorporates the use of cultural practices to reduce pathogen pressure, host resistance to disease, and biological approaches for stimulating plant growth and control of root diseases.

Hydrocarbons, Brominated↗

A geographic information system forecast model for strategic control of fasciolosis in Ethiopia.

A geographic information system (GIS) forecast model based on moisture and thermal regime was developed to assess the risk of Fasciola hepatica, a temperate species, and its tropical counterpart, Fasciola gigantica, in Ethiopia. Agroecological map zones and corresponding environmental features that control the distribution and abundance of the disease and its snail intermediate hosts were imported from the Food and Agriculture Organization (FAO) Crop Production System Zones (CPSZ) database on east Africa and used to construct a GIS using ATLAS GIS 3.0 software. Base temperatures of 10 degrees C and 16 degrees C were used for F. hepatica and F. gigantica, respectively, to calculate growing degree days in a previously developed climate forecast system that was modified to allow use of monthly climate data values. The model was validated by comparison of risk indices and environmental features to available survey data on fasciolosis. Monthly Fasciola risk indices of four climatic regions in Ethiopia were used to project infection transmission patterns under varying climatic conditions and strategic chemotherapeutic fasciolosis control schemes. Varying degrees of F. hepatica risk occurred in most parts of the country and distinct regional F. hepatica transmission patterns could be identified. In the humid west, cercariae-shedding was predicted to occur from May to October. In the south it occurred from April to May and September to October, depending on the annual abundance of rain. In the north-central and central regions, risk was highest during heavy summer rains and pasture contamination with metacercariae was predicted to occur during August-September, except in wet years, when it may start as early as July and extend up to October. At cooler sites above altitude of 2800 m, completion of an infection cycle may require more than a year. Fasciola gigantica risk was present in the western, southern and north-central regions of the country at altitudes of 1440-2560 m. However, a transmission cycle could be completed in a single year only at elevations below 1700 m. The greatest risk of F. gigantica infection was in the humid western region. Regional strategic chemotherapy schemes of two or three treatments per year were developed. Results suggest that the model can be extrapolated to all CPSZ in the country and adapted for use in control of other vector-borne diseases of economic and public health importance.

Animals↗

Use of Geographic Information Systems in the development of prediction models for onchocerciasis control in Ethiopia.

A risk assessment model was developed for onchocerciasis distribution and its control in Ethiopia using Geographic Information System (GIS) methods. GIS data analysis was done to generate 3 separate risk models using selected environmental features of (1) earth observing satellite data on Normalized Difference Vegetation Index (NDVI) and midday Land Surface Temperature (LST) prepared from AVHRR sensor data of the Global land 1-km project for the years 1992 and 1995, (2) FAO agroclimatic databases from the Crop Production System Zone (CPSZ) of the Intergovernmental Authority on Development (IGAD) sub-region of East Africa, and (3) a climate-based forecast index based on the growing degree days (GDD) and water budget concepts. Parasitological data used for the analysis were published and unpublished reports of onchocerciasis surveillance made between 1969 and 2000 in various parts of the country. Analysis of queries based on 1992 and 1995 annual wet and dry season data of the Global land 1-km project resulted in a predictive value of 95.1%, 94.0% and 96.3%, respectively, using data values extracted from buffers centered on sites above 5% prevalence. The model based on CPSZ data predicted an endemic area that best fit the distribution of sites over 5% prevalence; the query was based on CPSZ values of average altitude (442-2134 m), annual mean temperature (18-28 degrees C), annual rainfall (822-1980 mm), annual potential evapotranspiration (1264-1938 mm), rain minus potential evapotranspiration (-124 - 792 mm), average NDVI x 100 (2000-5000) and average terrain percent slope (9-34). The climate-based model based on GDD and water-budget predicted high risk to severe risk areas in the western and southwestern parts of the country. All three of the models predicted suitable areas for the transmission of onchocerciasis outside known endemic areas, suggesting the need for ground-based validation and potential application to current community-directed treatment programs with ivermectin (CDTI) for control of onchocerciasis in Ethiopia.

Agriculture↗

Genotypic and environmental effects on lupin seed composition.

Currently, white lupin (Lupinus albus L.) is gaining importance due to its high nitrogen fixation capability and potential in sustainable crop production systems. Even though research conducted in Australia, Chile, Germany, New Zealand, and Portugal has indicated lupin's positive potential as human and animal food, such information from Virginia and adjoining areas of the United States is not available. In addition, effects of growing environment and genotypes on lupin seed composition need to be characterized to evaluate lupin's potential as a food and feed crop. Towards this end, seed of 12 lupin genotypes produced in Maine (USA) and Virginia (USA) were compared to determine genotypic and environmental effects on contents of protein, sugar, oil, various fatty acids, amino acids, and minerals. The protein content of dry seed was not affected by growing environment. However, growing environment had significant effects on contents of total sugar, amino acids, oil, fatty acids, and minerals. Significant variation existed among 12 lupin genotypes for various traits when composition of seed produced in Virginia was evaluated. The results indicated that site-specific evaluation of adapted lupin genotypes for chemical composition should be included in efforts to evaluate lupin's overall potential as a food or feed crop.

Amino Acids↗