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PubMed2002

CSDBase: an interactive database for cold shock domain-containing proteins and the bacterial cold shock response.

CSDBase (http://www.chemie.uni-marburg.de/~csdbase/) is an interactive Internet-embedded research platform providing detailed information on proteins containing the cold shock domain (CSD). It consists of two separated database cores, one dedicated to CSD protein information, and one to provide a powerful resource to relevant literature with emphasis on the bacterial cold shock response. In addition to detailed protein information and useful cross links to other web sites, CSDBase contains computer-generated CSD structure models for most CSD-containing protein sequences available at NCBI non-redundant protein database at the time of CSDBase establishment. These models were calculated on the basis of known crystal and/or NMR structures using SWISS-MODEL and can be downloaded as PDB structure coordinate files for viewing and for manipulation with other software tools. CSDBase will be regularly updated and is organized in a compact form providing user friendly interfaces to both database cores which allow for easy data retrieval.

Amino Acid Sequence↗
PubMed1977

Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.

The matrix (M) protein of the H2N2 virus A/Ann Arbor/6/60 may be distinguished from M protein of several H3N2 viruses and A/New Jersey/76 (HSWINI) by SDS acrylamide gel electrophoresis using a discontinuous buffer system. The smallest RNA (RNA 8) of the A/Ann Arbor/6/60 virus may be distinguished from RNA 8 of several H3N2 viruses by acrylamide gel electrophoresis in 3% or 3-6% gels in the absence of urea, if electrophoresis is done at 30 to 36 degrees C or 20 degrees C respectively. Ten clones of conditionally-lethal temperature-sensitive (ts) mutants were studied, which derived their cold-adaption and ts genes from mutant A/Ann Arbor/6/60, and their haemagglutinin from the H3N2 virus A/Scotland/840/74. Each clone was found to derive its M protein from A/Ann Arbor/6/60 mutant, and its RNA 8 from A/Scotland/840/74. The only assignment of genes 7 and 8 consistent with these findings for the recombinants is that in each parent virus (and in the recombinants) gene 7 codes for M protein, and gene 8 for NS protein. Furthermore, it may be concluded from the results that the biologically important ts lesions in the A/Ann Arbor/6/60 mutant parent are not present in the NS gene. In addition to the recombinants of A/Ann Arbor/6/60 and A/Scotland/840/74, five independent ts/cold-adapted recombinants of A/Ann Arbor/6/60 mutant with H3N2 and HSWINI wild-type viruses were examined, and all were found to contain the M protein of the A/Ann Arbor/6/60 mutant parent. This is suggestive that M protein may be at least partially responsible for the cold-adaptation and/or ts properties of the A/Ann Arbor/6/60 mutant and the recombinants.

Adaptation, Biological↗
PubMed1999

The interaction between cold and light controls the expression of the cold-regulated barley gene cor14b and the accumulation of the corresponding protein.

We report the expression of the barley (Hordeum vulgare L.) COR (cold-regulated) gene cor14b (formerly pt59) and the accumulation of its chloroplast-localized protein product. A polyclonal antibody raised against the cor14b-encoded protein detected two chloroplast COR proteins: COR14a and COR14b. N-terminal sequencing of COR14a and expression of cor14b in Arabidopsis plants showed that COR14a is not encoded by the cor14b sequence, but it shared homology with the wheat (Triticum aestivum L.) WCS19 COR protein. The expression of cor14b was strongly impaired in the barley albino mutant an, suggesting the involvement of a plastidial factor in the control of gene expression. Low-level accumulation of COR14b was induced by cold treatment in etiolated plants, although cor14b expression and protein accumulation were enhanced after a short light pulse. Light quality was a determining factor in regulating gene expression: red or blue but not far-red or green light pulses were able to promote COR14b accumulation in etiolated plants, suggesting that phytochrome and blue light photoreceptors may be involved in the control of cor14b gene expression. Maximum accumulation of COR14b was reached only when plants were grown and/or hardened under the standard photoperiod. The effect of light on the COR14b stability was demonstrated by using transgenic Arabidopsis. These plants constitutively expressed cor14b mRNAs regardless of temperature and light conditions; nevertheless, green plants accumulated about twice as much COR14b protein as etiolated plants.

Amino Acid Sequence↗
PubMed1991

Effect of prior administration of cold saline on pain during propofol injection. A comparison with cold propofol and propofol with lignocaine.

A single-blind, randomised, controlled study was undertaken to compare the efficacy of three methods of preventing pain during injection of propofol on induction of anaesthesia. Patients were allocated randomly to receive unmodified propofol, propofol with 0.05% lignocaine, propofol at 4 degrees C and unmodified propofol preceded by 10 ml of 0.9% saline at 4 degrees C. Prior injection of cold saline reduced the incidence of pain and discomfort significantly (22%) compared with unmodified propofol (75%; p less than 0.005) and was similar to that after cold propofol (33%) and propofol with lignocaine (44%). There was no significant difference between the treatment groups.

Adult↗
PubMed1993

Waldenström's macroglobulinaemia presenting with cold urticaria and cold purpura.

A case of Waldenström's macroglobulinaemia presenting with non-specific dermatological symptoms (cold urticaria and cold purpura) is described in a 37-year-old female patient. Progressive telangiectasia and spider naevi were also observed. Involvement of the skin may be explained by the presence of macroglobulins with cryoprotein-like qualities. This facilitated an early diagnosis.

Adult↗
PubMed1973

Cold-sensitive Pseudomonas RNA polymerase. I. Characterization of the host dependent cold-sensitive restriction of phage CB3.

Analysis of bacteriophage CB3 infection of Pseudomonas aeruginosa strain PAT2 establishes that phage induced changes in net macromolecular synthesis are absent at nonpermissive phage growth temperatures (32 C). Alterations which are evident in the PAT2 strain at 37 C or in the fully permissive strain, PAO1C, at either warm or cold temperatures do not occur in PAT2 at low temperatures. CB3 DNA synthesis and the degradation of host DNA to approximately 78S components occur at 37 C, but are absent in PAT2 at 20 C. Nevertheless, attachment of phage DNA to host cytoplasmic material occurs under permissive and nonpermissive conditions. This binding of phage DNA at 20 C is identical in nature to phage DNA bound at 37 C. Thus, the conditional cold-sensitive PAT2 host function in the growth of CB3 is expressed subsequent to membrane binding of the infecting genomes but prior to the onset of the initiation of CB3 DNA synthesis, the inhibition of host DNA synthesis, and the transient depression in RNA synthesis which occurs in permissive cells.

Bacterial Proteins↗
PubMed1991

Heat debt during cold air exposure before and after cold water immersions.

Acclimation to cold can manifest itself in several different ways, insulative and metabolic being the most common. Bittel (J. Appl. Physiol. 62: 1627-1634, 1987) has demonstrated that heat debt, which encompasses both heat production and heat loss, can be used as a unitary index of acclimation. However, conflicting results are obtained if heat debt is calculated using a mean-weighted body temperature (Tb) vs. the change of body heat content through the integration of heat storage (S). The present study examines the determination of heat debt by three methods of calculation, the first based on Tb and the other two based on S where heat losses are measured in one and predicted in the other. Data were obtained from five healthy young males exposed to 10 degrees C air for 2 h on four different occasions. The first two exposures provided control data, while the last two were performed after 5 and 10 days, respectively, of daily immersions in 15 degrees C water to induce acclimation. The variability in response between the control exposures was as large as that among the other exposures. Although the method of calculation using Tb indicated that subjects were close to a thermal balance after 2 h of cold air exposure, this contrasted sharply with the result of the other two methods that indicated heat debt was still increasing steadily. The latter two methods are considered more accurate for transient heat debt calculation. Although cases of individual acclimation were found, these were different among the subjects, resulting in pooled responses that indicated no group acclimation by means of any of the three methods of calculation.(ABSTRACT TRUNCATED AT 250 WORDS)

Adaptation, Physiological↗
PubMed1977

Cold urticaria. Recognition and characterization of a neutrophil chemotactic factor which appears in serum during experimental cold challenge.

Sera were obtained from the venous effluents of cold-challenged arms of patients with idiopathic cold urticaria without plasma or serum cryoproteins; these sera exhibited increased neutrophil chemotactic activity without alterations of the complement system. A two- to fourfold augmentation of the base-line neutrophil chemotactic activity of serum from the immersed extremity began within 1 min, peaked at 2 min, and returned to base-line levels within 15 min, whereas there was no change in the serum chemotactic activity in the control arm. The augmented chemotactic activity in the serum specimens from the challenged arm of each patient appeared in a high molecular-weight region, as assessed by the difference in activity recovered after Sephadex G-200 gel filtration of the paired lesional and control specimens. Sequential purification of this high molecular-weight activity by anion- and cation-exchange chromatography revealed a single peak of activity at both steps. The partially purified material continued to exhibit a high molecular weight, being excluded on Sepharose 4B, and had a neutral isoelectric point. The partially purified material showed a preferential chemotactic activity for neutrophilic polymorphonuclear leukocytes, required a gradient for expression of this function, and exhibited a capacity to deactivate this cell type. This active principle, termed high molecular-weight neutrophil chemotactic factor, exhibited a time-course of release that could be superimposed upon that of histamine and the low molecular-weight eosinophil chemotactic factor and may represent another mast cell-derived mediator.

Chemotaxis, Leukocyte↗
PubMed1997

Raynaud's phenomenon in vibration syndrome: the impact of cold feet on skin temperature and vasomotion of the hand after immersion in cold water.

Patients with vibration syndrome, suffering from Raynaud's phenomenon, are sensitive to cold. Rewarming time, after local cooling, is delayed. The present study evaluated whether rewarming of the hand after cooling is influenced by the temperature of the feet. In five Japanese patients (former forest workers) with vibration syndrome, suffering from Raynaud's phenomenon, and in five healthy controls, temperature changes of the hand after cooling were registered under the two test situations (on different days) with the feet immersed in water of 35 degrees C or 20 degrees C, respectively. In both patients and controls (in both groups, in four of five cases) rewarming of the hand after cooling was faster when the feet were immersed in cold water, compared with when the feet were immersed in warm water. In this test situation, the systemic thermoregulative counterreaction appears to be more important for rewarming of the hand after cooling than a possible synchronous passive reaction accompanying warming of the feet. A deliberate training of the systemic counterreaction may prove beneficial for patients with Raynaud's phenomenon.

Adult↗
PubMed1995

Changes in motoneuron membrane potential and reflex activity induced by sudden cooling of isolated spinal cords: differences among cold-sensitive, cold-resistant and freeze-tolerant amphibian species.

The effects of sudden cooling of the spinal cord were studied in three species of amphibians--a cold-sensitive tropical toad (Bufo marinus), a cold-resistant, aquatic, hibernating frog (Rana pipiens, northern leopard frog) and a freeze-tolerant frog (Rana sylvatica, wood frog). Ventral root (motoneuron) potentials were recorded from isolated, hemisected spinal cords of each species mounted in a sucrose-gap recording apparatus and superfused with HCO3(-)-buffered Ringer's solution at room temperature (21 degrees C). In the toad, sudden cooling to 6-8 degrees C produced large, sustained motoneuron depolarizations that returned slowly to baseline levels and were accompanied by extensive paroxysmal activity. Larger, but shorter-lasting, motoneuron depolarizations associated with only a limited amount of paroxysmal activity were generated by rapid cooling of the leopard frog spinal cord. Small, brief motoneuron depolarizations followed by a hyperpolarization, or hyperpolarizations not preceded by depolarizations, were seen in cooled wood frog spinal cords. The wood frog displayed a large amount of spontaneous motoneuron activity, but little paroxysmal activity in response to sudden cooling. Following prolonged cooling, rewarming the spinal cords of all three species resulted in motoneuron hyperpolarizations that slowly decayed towards the baseline value. The amplitude of the rewarming-induced response was larger and longer in toad motoneurons than in leopard frog and wood frog motoneurons. At room temperature, a single supramaximal dorsal root stimulus evoked a depolarizing ventral root potential in toad and leopard frog motoneurons that was decreased in amplitude and prolonged when the spinal cords were cooled to 8 degrees C or below. In contrast, at room temperature, the ventral root reflex in the wood frog was followed by a distinct hyperpolarization. Cooling the wood frog spinal cord only slightly reduced the amplitude of the ventral root potential. In contrast, the evoked hyperpolarization was blocked by sudden cooling and also by the addition of dihydro-ouabain to the Ringer's solution. The motoneuron hyperpolarizations induced by sudden cooling in the wood frog were converted to depolarizations when Cl- in the superfusate was replaced with isethionate. The depolarizations elicited by sudden cooling were reduced by the addition of kynurenate in all three species. A dose-response curve generated by short applications of L-glutamate demonstrated that wood frog motoneurons were less sensitive than leopard frog motoneurons to L-glutamate. In summary, three species of amphibians, differing in their adaptations to the temperature of their environments, vary in their responses to sudden reductions in temperature. The relationship of these responses to their environmental adaptations remains to be determined.

Animals↗
PubMed1992

[Waldenstrm macroglobulinemia presenting in the form of cold urticaria and cold purpura].

A Waldenström macroglobulinemia case, manifested in the form of non-specific skin-symptoms (cold urticaria and cold purpura) is described in a 37 years old female patient. As new skin-symptoms progressive telangiectasia and naevi aranei could be observed. Involvement of skin may be explained by macroglobulins with kryoprotein properties, making early diagnosis possible at the same time.

Adult↗
PubMed1988

Effects of cold acclimation, cold exposure, and palatability on postprandial thermogenesis in rats.

Groups of rats were maintained at 24 degrees C and 4 degrees C (WA, CA). At each temperature one group was fed a pelleted stock diet, while another was fed in addition, 3 kJ of palatable food at 1-h intervals for a total of 8 h during the day. After 2 weeks the thermogenic response to both 3 kJ and 30 kJ of palatable food was determined by measuring oxygen consumption over a period of 3 h. Food intake revealed two phases of postprandial thermogenesis. During the initial cephalic phase which lasted 30 min the O2 consumption increased by about 40 percent with both test meals and the response was comparable for WA and CA rats tested at the same temperature. During the subsequent gastrointestinal phase the response was proportional to the size of the meal and the CA rats were always more responsive than the WA rats specially when tested at 4 degrees C. The results suggest that postprandial thermogenesis during the initial cephalic phase is related to food palatability and that cold exposure and cold acclimation cause an enhanced response during the second phase of meal thermogenesis.

Acclimatization↗
PubMed1987

[Effect of cold adaptation and brief exposure to cold on the resistance of animals to hypoxic hypoxia].

Cold adaptation of white rats (45 days, 2 degrees C) considerably increased their resistance against hypoxic hypoxia as indicated by an increase of life duration under hypoxia, a decrease in seizure frequency, and the absence of changes in monoamine oxidase catalytic properties typical for hypoxia. Cold stress, however, reduced the animals resistance against hypoxic hypoxia.

Acclimatization↗
PubMed1979

[Studies on medico-legal diagnosis in cold district. 3. Autopsy findings of the victims of cold exposure (author's transl)].

The authors have investigated the autopsy findings of 8 bodies died from cold. The conclusions are as follows: Pink colour of the blood in the left ventricle or the pulmonary veins, pink colour of the lungs and pink patches of the skin are fairly pathognomic findings of death from cold. Seven out of eight bodies have presented at least one of these findings. Subserous or submucous hemorrhage is less pathognomic and has been presented less frequently.

Adult↗
PubMed1977

Cold-induced vasodilatation and peripheral blood flow under local cold stress in man at altitude.

The cold-induced vasodilatation (CIVD) response was studied on 17 lowlanders (20-30 years) at Delhi, using a water bath maintained at 4 degrees+/-0.2 degreesC. The temperatures were measured at the tip of the index finger, centre of the palm, over a prominent wrist vein, and orally. Then the subjects were air-lifted to an altitude of 3500 m, where the measurements were repeated at weekly intervals for a period of 3 weeks. After this, they were flown back and retested. For comparison, the study at altitude was done on 10 acclimatised lowlanders and on 10 high-altitude natives. The peripheral blood flow under a local cold stress was calculated. Three types of CIVD responses--viz. typical hunting, proportional control, and continuous rewarmin--were observed. At altitude, the pattern was more or less the same but there was a significant reduction in the response. The responses of the natives were more or less identical to those of the lowlanders at sea level, and responses of the acclimatized lowlanders were better than the fresh inductees, but much less than the natives. The changes in peripheral blood flow was in accordance with the CIVD response.

Acclimatization↗
PubMed1995

[The effect of EDTA on the impulse activity of cold thermoreceptors and mechanical cold receptors in rabbits under deep skin cooling].

The EDTA restored the bioelectrical activity and increased its rate in majority of the skin cold thermoreceptors and mechano-cold receptors of the nasolabial area after deep cooling of the skin in anesthetised rabbits. The data obtained suggest that a decrease in the calcium level in the blood lowers the temperature thresholds of the activity of these receptors.

Animals↗
PubMed1999

Photoassociative Spectroscopy and Formation of Cold Molecules in Cold Cesium Vapor: Trap-Loss Spectrum versus Ion Spectrum.

We report on the experimental spectra of all the optically accessible long-range attractive molecular states of the Cs2 dimer below the 6s2S1/2 + 6p2P3/2 dissociation limit by molecular photoassociation of cold Cs atoms. The spectra are obtained by the usual trap-loss method as well as by pulsed-laser photoionization of Cs2 molecules into Cs+2 ions. The two spectra present markedly different features. While the 1g, 0(+)u, and 0(-)g vibrational progressions are present in the trap-loss spectrum, the Cs+2 ion spectrum presents only the 0(-)g and 1u vibrational progressions. Those states (0(-)g and 1u) lead to the formation of the translationally cold Cs2 ground state molecules at temperatures in the 100 µK range, to our knowledge the lowest molecular temperature reported up until now. The C3 asymptotic coefficients for the 0(+)u and 1g states are determined through a fit of the experimental energy levels. Copyright 1999 Academic Press.

Journal Article↗
PubMed1989

[Comparison of cold blood cardioplegia and cold crystalloid cardioplegia with or without magnesium].

Blood cardioplegia has recently been advocated as a superior method of myocardial protection, but its temperature is important, and it has not been compared to a magnesium containing crystalloid cardioplegia. This study was undertaken to compare the protective effect of cold blood cardioplegia (BCP) and cold crystalloid cardioplegia with or without magnesium (MgKCP and KCP). Fourty-five patients were undertaken prosthetic cardiac valve replacement with left ventricular volume overload from valvular regurgitation. They were divided into three groups for the difference of the cardioplegic solution. Intraoperatively, myocardial temperature and the tissue PCO2 was monitored, and the content of calcium and magnesium of right atrium was measured before and after aortic cross clamping. Postoperatively, left ventricular stroke work index and serum CPK-MB isozyme activity were measured at 3, 6, 24 and 48 hours after weaning from cardiopulmonary bypass. The increase of the tissue PCO2 during aortic cross clamping and its recovery at ten minutes after reperfusion were significantly suppressed in BCP. The retension of the tissue calcium after reperfusion and calcium to magnesium ratio were significantly low in MgKCP. The recovery of cardiac pump function and decrease of CPK-MB were superior in MgKCP.

Cardioplegic Solutions↗