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Attachment theory and brief psychotherapy: applying current research to clinical interventions.

Recent advances in attachment research provide a framework for defining the content and process of brief psychotherapy with adults. Attachment theory emphasizes a number of issues crucial to therapeutic work. Specifically, attachment relationships are enduring components of a individual's pattern of interpersonal behaviours. Functionally, attachment relationships address security needs. Insecure attachment arises from a representational model based on feared loss of the attachment figure, which predisposes the individual to have little confidence in the attachment figure's availability, responsiveness, and permanence. Behavioural responses to insecure attachment can lead to specific patterns of interpersonal relationships which, in turn, strengthen the representational model. Thus, a relatively stable, self-reinforcing system evolves and results in a consistent inability to experience security within attachment relationships. In this article, the authors describe the current framework for understanding adult attachment relationships and present clinical vignettes illustrating the saliency of attachment theory to common clinical presentations. The goal of the clinical intervention is defined as increasing the "permeability" of the individual's working model of attachment through affective and cognitive re-assessment of attachment experiences and expectations. Finally, the utility of this approach in evaluating the outcome and effectiveness of psychotherapy is highlighted.

Adult↗

Modulation of integrin-mediated attachment of chondrocytes to extracellular matrix proteins by cations, retinoic acid, and transforming growth factor beta.

Articular cartilage chondrocytes are surrounded by an abundant extracellular matrix (ECM) and the interactions between chondrocytes and ECM proteins have important effects on chondrocyte function. In this study, attachment assays were used to examine integrin-mediated attachment of chondrocytes to fibronectin, matrix Gla protein (MGP), and type II collagen. A cyclic peptide containing the Arg-Gly-Asp integrin recognition sequence was capable of blocking attachment of chondrocytes to all three ECM proteins including collagen. Cations were required for chondrocyte attachment and provided a mechanism to differentially regulate attachment to ECM proteins. Mg2+ and Mn2+ supported attachment to all three ECM proteins but Ca2+ did not support attachment to collagen and was less effective than Mg2+ or Mn2+ in chondrocyte attachment to MGP. Treatment of chondrocytes with retinoic acid for the first 3 days of culture, prior to use in attachment assays, resulted in a decrease in the attachment to collagen but not to fibronectin or MGP. Seven days of retinoic acid treatment resulted in decreased attachment to fibronectin, MGP, and collagen. Treatment with 100 pM TGF-beta for 18 h stimulated attachment to all three proteins. TGF-beta treatment was able to at least partially overcome the inhibitory effects of retinoic acid. These results demonstrate several important mechanisms by which chondrocyte-ECM interactions may be modulated. The differential effects of retinoic acid and TGF-beta on attachment correlate with their known effects on matrix synthesis, which suggests a link between matrix synthesis and integrin expression in chondrocytes.

Animals↗

Attachment types of the long head of the biceps tendon to the glenoid labrum and their relationships with the glenohumeral ligaments.

PURPOSE: To clarify the attachment types of the long head of the biceps tendon to the glenoid labrum and their relationships with the glenoid attachment of the glenohumeral ligaments. TYPES OF STUDY: Anatomic study in cadavers. METHODS: Using 101 cadaver shoulders, the glenoid attachment types of the biceps tendon and the location of the glenoid origin of the glenohumeral ligaments were assessed macroscopically and then histologically on the sagittal section. RESULTS: The glenoid attachment types of the biceps tendon were defined as entirely-posterior in 28 shoulders (27.7%), posterior-dominant in 56 shoulders (55.4%), equal in 17 shoulders (16.8%), and entirely anterior in none (0%). In all shoulders, the superior and middle glenohumeral ligaments were attached between 12 and 1 o'clock (average, 12:36 o'clock), whereas the inferior glenohumeral ligament (IGHL) showed greater variation. In the posterior-dominant and equal types, the IGHL was attached relatively high (average 2:49 o'clock for the posterior-dominant type and 1:49 o'clock for the equal type). In the entirely posterior type, the IGHL was attached low, between 4 and 5 o'clock (average, 4:32 o'clock). There was a significant difference between them (P < .001). Histologic examination showed that in the posterior-dominant and equal types, the fibers of the IGHL directly linked to the fibers of the biceps tendon, whereas in the entirely posterior type no such direct connections were observed. However, in all types, the fibers of the biceps tendon were attached to the posterior labrum. CONCLUSIONS: The labral attachment of the long head of biceps tendon was posterior regardless of its macroscopic appearance. The macroscopic attachment pattern of the biceps tendon resulted from the different attachment height of the IGHL. The attachment site of the IGHL is lower than 4 o'clock in the entirely posterior type, whereas it is higher than 4 o'clock in other types. CLINICAL RELEVANCE: The present study provides useful information about the original attachment site of the IGHL during Bankart repair.

Anthropometry↗

Time dependent properties of bovine meniscal attachments: stress relaxation and creep.

It has been suggested that the success of a meniscal replacement is dependent on several factors, one of which is the secure fixation and firm attachment of the replacement to the tibial plateau [Chen, M.I., Branch, T.P., et al., 1996. Is it important to secure the horns during lateral meniscal transplantation? A cadaveric study. Arthroscopy 12(2), 174-181; Alhalki, M.M., et al., 1999. How three methods for fixing a medial meniscal autograft affect tibial contact mechanics. American Journal of Sports Medicine 27(3), 320-328; Haut Donahue, T.L., et al., 2003. How the stiffness of meniscal attachments and meniscal material properties affect tibio-femoral contact pressure computed using a validated finite element model of the human knee joint. Journal of Biomechanics 36(1), 19-34]. The complex loading environment in the knee lends itself to different loading environments for each meniscal attachment. We hypothesize that the creep and stress relaxation characteristics of the horn attachments will be different for the anterior versus posterior, and medial versus lateral attachments. To test this hypothesis, the stress relaxation and creep characteristics of the meniscal horn attachments were determined. The stress relaxation properties of load/stress at the end of the test, and the load/stress relaxation rate demonstrated no significant statistical differences between the attachments. Unlike the stress relaxation properties, the creep properties demonstrated some significant differences amongst the attachments. The normalized displacement at the end of the test, normalized creep rate and strain creep rate for the lateral anterior attachment were significantly different than those of the medial posterior attachment (p<0.05). The two anterior attachments had significantly different strains at the end of the test, as well as significantly different creep strain rates (p<0.05). The two attachments of the medial meniscus revealed no significant differences between any of the creep properties measured (p>0.05). The time dependent properties obtained in this experiment provide insight into the behavior of meniscal horn attachments under various loading situations. The results indicate that a suitable meniscal replacement may require different properties for the lateral and medial horns.

Animals↗

Variations in holdfast attachment mechanics with developmental stage, substratum-type, season, and wave-exposure for the intertidal kelp species Hedophyllum sessile (C. Agardh) Setchell.

Biomechanical models that describe physical and biological interactions on wave-exposed shores typically assume that a species' attachment properties are similar between seasons and sites. We tested this assumption using Hedophyllum sessile to investigate how macroalgal biomechanical attachment properties vary with developmental stage, substratum-type, season, and wave-exposure. Hedophyllum sessile is an intertidal kelp species that is able to survive in wave-exposed areas in the Northeast Pacific. For both juveniles and adults, holdfast attachment force and strength were measured at a wave-exposed and wave-protected site in Barkley Sound, British Columbia, Canada. Substratum and wave-exposure effects on attachment properties were tested in juvenile populations. Adult populations were sampled prior to (in July 1996) and after (in November 1996) a series of storms. Site and seasonal wave-exposure effects on attachment properties were tested in these adult populations. Comparisons to known attachment properties of other temperate macroalgal species were also made. Causes for these patterns are discussed but were not isolated in these studies. Juveniles' attachment properties differed on different substrata types and between wave-exposures, with the highest attachment forces and the most attached juveniles in articulated coralline algal turfs. Adult attachment is firm ( approximately 100 N), but relatively weak ( approximately 0.07 MNm(-2)). Adult attachment did not vary with site wave-exposure, but there was a shift within each site to more resistant holdfasts after a series of early winter storms. Seasonal increases in storm swells correlated to more thallus tattering and selected against large, loose holdfasts. The data presented here suggest that results from holdfast attachment field studies in one season cannot be extrapolated to another due to a complex set of dynamics. This is the first documentation of seasonal patterns in macroalgal attachment properties.

Journal Article↗

Attachment and coping with chronic disease.

BACKGROUND: In this clinical study, attachment theory was applied to research in the field of coping with chronic disease. The approach was to integrate concepts of coping within a framework of attachment theory. It was hypothesised that attachment concepts have an influence on coping strategies, and that they may predict the subjective emotional and physical health status during the course of medical treatment. METHOD: One hundred fifty patients were investigated with an adult attachment interview (AAPR coding system) and a coping interview (Bernese Coping Modes). Self-reported coping modes, social support, the subjective health status and quality of life were also assessed by self-report measures at two or more sampling points of measurement. Three subsamples of patients, suffering from (a) breast cancer, (b) chronic leg ulcers and (c) alopecia, were studied in order to include a broad range of subjective impairment caused by a disease. RESULTS: Findings indicate a moderate statistical effect of attachment patterns on coping strategies when controlling the influence of confounding variables. Insecure attachment was related to less flexible coping. Coping strategies also differed between the different types of insecure attachment; however, there were differences depending on the perspective of the coping behaviour (self- vs. observer ratings) as well. From the observer perspective, ambivalently attached individuals showed more negative emotional coping while avoidantly attached individuals showed more diverting strategies. In the self-report, ambivalently attached patients revealed hyperactivating tendencies in their coping behaviour while avoidantly attached individuals revealed deactivating tendencies. CONCLUSION: As a conclusion, two levels of coping should be differentiated. One level strongly corresponds with affect regulation, in particular the regulation of attachment-related emotions and concerns, while the other level shows a stronger tendency to outwardly oriented coping. A more secure attachment might be considered to be an important inner resource in the emotional adaptation to chronic diseases.

Adaptation, Psychological↗

Enhanced attachment of acanthamoeba to extended-wear silicone hydrogel contact lenses: a new risk factor for infection?

PURPOSE: To establish if silicone hydrogel (S-H) contact lenses could be a risk factor for Acanthamoeba infection by facilitating the attachment of trophozoites to their surface and transfer to the cornea and to determine the effect Acanthamoeba culture technique, patient wear, and Pseudomonas biofilm coating have on attachment to the S-H lens. DESIGN: Experimental material study. PARTICIPANTS AND CONTROLS: Attachment to a S-H lens was compared with that of a conventional hydrogel control lens. Sixteen replicates were carried out for both lens types under each test condition. METHODS: Unworn S-H (PureVision; Bausch & Lomb, Kingston-Upon-Thames UK) and conventional hydrogel (Acuvue; Vistakon, Johnson & Johnson, Jacksonville, FL USA) lens quarters were incubated for 90 minutes in suspensions of liquid or plate-cultured Acanthamoeba castellanii trophozoites. Unworn, worn, and Pseudomonas biofilm coated S-H and hydrogel quarters were incubated for 90 minutes with plate-cultured trophozoites. MAIN OUTCOME MEASURES: Trophozoites attached to one surface of each lens quarter were counted by direct light microscopy. Logarithmic transformation of data allowed the use of a parametric analysis of variance. RESULTS: Lens polymer had a significant effect on attachment (P < 0.001), with higher numbers of trophozoites attaching to the S-H lens. Culture technique also had a significant effect on attachment (P = 0.013), with higher numbers of liquid-cultured organisms attaching to both lens types. A significant increase in attachment was demonstrated with worn and Pseudomonas biofilm-coated hydrogel lenses (P < 0.001); however, this difference was not seen with the S-H lens. CONCLUSIONS: Acanthamoebal attachment to the S-H lenses was significantly greater than to the conventional hydrogel. Liquid-cultured trophozoites demonstrated a higher affinity for the lenses tested. Wear and bacterial biofilm coating had no effect on attachment to S-H lenses. The increased attachment found with the S-H lens may be an inherent characteristic of the polymer or a side effect of the surface treatment procedure to which the lenses are exposed. It is possible that S-H lenses are at greater risk of promoting Acanthamoeba infection if exposed to the organism because of the enhanced attachment characteristic of this new material.

Acanthamoeba↗

Salicylate inhibition of Acanthamoeba attachment to contact lenses: a model to reduce risk of infection.

PURPOSE: Acanthamoeba attachment (adsorption) to hydrogel contact lenses is enhanced by Pseudomonas aeruginosa biofilm. The effect of sodium salicylate on Acanthamoeba attachment to biofilm-coated and uncoated hydrogel lenses was investigated. DESIGN: Experimental study. PARTICIPANTS AND CONTROLS: A minimum of 16 replicates were used for each test condition; a control condition using clean lenses without biofilm was included. METHODS: Four groups of hydrogel contact lenses (etafilcon A) were pretreated with P. aeruginosa to form a biofilm. In addition, two more groups remained untreated. Quartered lenses of all six groups were then incubated in a suspension of A. castellanii trophozoites. Two batches of lenses had either 3 or 30 mM sodium salicylate added to the bacterial suspension (stage 1 intervention). Two other batches of lenses had salicylate added to the amoebal suspension (stage 2 intervention). One of the batches, which had a stage 1 intervention, had salicylate added at the second stage as well. The remaining batches received no salicylate exposure and included lenses with and without biofilm coating. MAIN OUTCOME MEASURE: The outcome measure in this study was the number of Acanthamoeba trophozoites attached, per square centimeter, to the hydrogel surfaces. RESULTS: Biofilm coating from P. aeruginosa gave a significantly increased attachment of A. castellanii trophozoites to the contact lens. When introduced at a first (biofilm) stage, second (trophozoite attachment) stage, or with intervention at both stages, 30 mM sodium salicylate reduced amoebal attachment to the hydrogel lens. When applied to both stages and when applied at stage 2 to the biofilm coated contact lenses, 3 mM sodium salicylate reduced amoebal attachment. The 3 mM concentration was not effective for the lower level of amoebae attachment to uncoated (nonbiofilm) lenses. CONCLUSIONS: Sodium salicylate successfully reduced amoebal trophozoite attachment to hydrogel lenses. This was the result of one of the following possibilities or a combination thereof: inhibition of biofilm formation; a direct effect on the amoebae; an alteration in the biofilm-amoebal attachment and resulting modification of the hydrogel lens surface. The results of this study suggest the major action is at stage 2 (on amoebal attachment to lenses) and favors alteration of the biofilm-amoebal attachment mechanism. This study demonstrates salicylate's potential benefit as a component of contact lens care solutions, designed to reduce microbial attachment and the risk of infection.

Acanthamoeba↗

[Attachment security and quality of life in atopic dermatitis].

OBJECTIVE: Attachment style is an important variable of influence on general and disease specific quality of life. Insecure attachment may go along with increased autonomic vulnerability and with compromised social support and coping resources. Patients with atopic dermatitis often report interactions of the status and severity of their disease with psychic wellbeing. It was the purpose of the present study to clarify the influence of attachment style and social support on the quality of life in probands with atopic dermatitis. We hypothesized that securely attached test persons with severe skin affection feel less constrained in their quality of life, because they may "buffer" strains from the disease more successfully due to better social support deriving from more satisfying relationships. METHOD: We carried through a cross-sectional study on 124 probands with atopic dermatitis of varying severity, assessed with self report (Neurodermitis Severity Index, NSI) and in part by clinical dermatological ratings (NSI/SCORAD). Attachment style was captured with the Relationship Scales Questionnaire (RSQ), social support with the F-SOZU (K-22), and quality of life with the Skindex-29. RESULTS: We found slightly more insecurely attached individuals in the study sample than it was to be expected from representational healthy samples. Securely attached participants have no lower severity of their disease, but show a higher amount of social support. Irrespective of severity, securely attached persons feel less limited in their quality of life compared to insecurely attached participants. In their symptom based quality of life both feel equally limited. Severity predicts quality of life: contrary to our expectation of a buffering effect of attachment security this association is closer in securely attached probands. CONCLUSION: Suffering from atopic dermatitis may go along with more attachment insecurity. The unexpected stronger association between severity and quality of life in securely attached individuals can be interpreted as a more direct reaction to their skin condition in the absence of other encumbering factors (relationship problems etc.).

Adaptation, Psychological↗

The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.

This study was undertaken to determine the roles of individual alpha/beta 1 integrin heterodimers in promoting cellular interactions with the different attachment-promoting domains of laminin (LN). To do this, antibodies to the integrin beta 1 subunit or to specific integrin alpha subunits were tested for effects on cell attachment to LN, to elastase fragments E1-4 and E1, derived from the short arms and core of LN's cruciform structure, and to fragment E8 derived from the long arm of this structure. The human JAR choriocarcinoma cells used in this study attached to LN and to fragments E1 and E8. Attachment to E1-4 required a much higher substrate coating concentration, suggesting that it is a poor substrate for JAR cell attachment. The ability of cells to attach to different LN domains suggested the presence of more than one LN receptor. These multiple LN receptors were shown to be beta 1 integrin heterodimers because antibodies to the integrin beta 1 subunit inhibited attachment of JAR cells to LN and its three fragments. To identify the individual integrin alpha/beta 1 heterodimers that mediate interactions with these LN domains, mAbs specific for individual beta 1 heterodimers in human cells were used to study JAR cell interactions with LN and its fragments. An anti-alpha 6/beta 1-specific mAb, GoH3, virtually eliminated cell attachment to E8 and partially inhibited attachment to E1 and intact LN. Thus the major alpha 6/beta 1 attachment domain is present in fragment E8. An alpha 1/beta 1-specific mAb (S2G3) strongly inhibited cell attachment to collagen IV and partially inhibited JAR attachment to LN fragment E1. Thus, the alpha 1/beta 1 heterodimer is a dual receptor for collagen IV and LN, interacting with LN at a site in fragment E1. In combination, the anti-alpha 1- and anti-alpha 6-specific antibodies completely inhibited JAR cell attachment to LN and fragment E1. Thus, the alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers each function as LN receptors and act together to mediate the interactions of human JAR choriocarcinoma cells with LN.

Antibodies, Monoclonal↗

Plaque-induced gingival inflammation in the absence of attached gingiva in dogs.

The purpose of this experiment was to evaluate the effect of plaque infection on gingival units (1) with or without support of attached gingiva and (2) with different height of the attachment apparatus. 7 beagle dogs were used. Prior to the initiation of the study 4 different types of "dentogingival" units had been established in each dog by the use of excisional and grafting procedures, namely (1) normal non-operated free gingival units supported by a wide zone of attached gingiva and normal height of the attachment apparatus, (2) regenerated free gingival units supported by loosely attached alveolar mucosa and normal height of the attachment apparatus, (3) regenerated free gingival units supported by loosely attached alveolar mucosa and reduced height of the attachment apparatus and (4) regenerated free gingival units supported by a wide zone of attached gingiva and reduced height of the supporting apparatus. A baseline examination involved assessments of plaque, gingivitis, gingival exudate, probing depth, clinical attachment level, position of the "soft tissue margin" and width of attached gingiva. Following this examination 2 of the dogs were scheduled for biopsy and sacrificed. The remaining 5 dogs were for 40 days placed on a diet regimen which allowed plaque accumulation. The clinical examination was repeated and biopsies sampled at the end of this period. Following preparation the biopsy material was subjected to histometric and morphometric analysis. The results showed that the free gingiva which regenerated following surgical excision of the entire gingiva or following soft tissue grafting, was in most respects, clinically as well as histologically, similar to the "normal" free gingiva. The data obtained after 40 days of plaque accumulation did not reveal any differences between the various "dentogingival" units regarding size and apical extension of the infiltrated portion of the connective tissue. It was concluded that a free gingival unit which is supported by loosely attached alveolar mucosa is not more susceptible to inflammation than a free gingival unit which is supported by a wide zone of attached gingiva.

Animals↗

Attachment of Actinomyces naeslundii to human buccal epithelial cells.

A standardized assay was used to measure the attachment of Actinomyces naeslundii ATCC 12104 to washed human buccal epithelial cells. Treatment of the A. naeslundii cells with hyaluronidases, wheat germ lipase, protease, trypsin, heat, or sonic oscillation significantly reduced their ability to attach to epithelial cells. Treatment of the epithelial cells with the above enzymes did not influence the attachment of A. naeslundii. Extraction of A. naeslundii with NaOH also significantly reduced the ability of the bacterium to attach to human buccal epithelial cells. The neutralized and dialyzed NaOH extract contained both carbohydrate and protein substances in a ratio of about 1:1. Adding this extract back to the extracted bacterial cells partially restored their ability to attach to epithelial cells. When the NaOH extract was preincubated with epithelial cells and residual extract was removed by washing, attachment of normal A. naeslundii was partially blocked. The ability of the extracted material to block attachment was significantly reduced when treated with hyaluronidases or with wheat germ lipase. Treatment with heat, protease, or trypsin did not significantly reduce the ability of the extracted materials to block attachment. Pretreatment of the epithelial cells with hyaluronic acid or chondroitin sulfate also reduced subsequent attachment of normal A. naeslundii cells. Pretreatment of epithelial cells with dextrans, proteins, or unpure mannose did not influence subsequent attachment of A. naeslundii. Pretreatment of A. naeslundii with galactose and lactose significantly inhibited attachment to normal epithelial cells. The results suggest that the attachment of A. naeslundii to human buccal epithelial cells may involve mucopolysaccharides similar to hyaluronic acid on the surface of the bacterial cells. Other attachment mechanisms may also be operative.

Actinomyces↗

Pneumocystis carinii attachment increases expression of fibronectin-binding integrins on cultured lung cells.

Pneumocystis carinii is an extracellular pathogen which requires attachment to alveolar epithelial cells for growth and replication. Previous studies have demonstrated that the extracellular matrix protein fibronectin (Fn) facilitates attachment of P. carinii to lung cells. This study addresses the role of cell surface Fn receptors (integrins) as mediators of P. carinii attachment and demonstrates the effect of P. carinii attachment on integrin expression on cultured lung cells. To determine the role of Fn-binding integrins in P. carinii attachment, attachment of 51Cr-labelled P. carinii organisms to the lung epithelial cell line A549 was quantified in the presence or absence of anti-integrin antibodies. Antibodies to the alpha v and alpha 5 integrin subunits significantly inhibited P. carinii attachment, while addition of antibody to the alpha subunit of a non-Fn-binding integrin, alpha 2, did not affect P. carinii attachment. To further investigate the role of Fn-binding integrins in P. carinii attachment, the effect of P. carinii attachment on expression of the alpha v and alpha 5 integrin subunits was determined. A549 cells incubated with either P. carinii organisms or with the P. carinii major surface glycoprotein gp120 demonstrated a 3- to 10-fold increase in expression of the alpha 5 integrin subunit; however, neither P. carinii nor gp120 affected the expression of alpha v integrin. Furthermore, the effects of P. carinii on A549 cell alpha 5 integrin expression were attenuated by the addition of an anti-gp120 antibody which blocks P. carinii attachment to A549 cells. Therefore, P. carinii attachment to lung epithelial cells appears to be mediated by alpha v- and alpha 5-containing integrins expressed on the epithelial cell surface, and P. carinii attachment results in increased expression of the alpha 5 integrin subunit.

Animals↗

Fear of intimacy in women: relationship between attachment styles and depressive symptoms.

BACKGROUND: Preoccupied and fearful attachment have both been associated with depressive symptoms, but it is unclear which attachment style is primarily associated with major depression. While preoccupied attachment denotes a focus on relationships as a source of self-validation, fearful attachment involves an avoidance of relationships for fear of rejection. Present research aims to investigate the relative influence of fearful and preoccupied attachments on self-reported depressive symptoms within both a student and a clinical sample and to examine these associations separately for male and female participants. SAMPLING AND METHODS: All participants completed the Relationship Questionnaire and the Beck Depression Inventory. Study 1 assessed attachment ratings and self-reported severity of depression for 71 patients diagnosed with major depression. Study 2 similarly assessed 245 university students, and study 3 compared attachment ratings of the two samples. RESULTS: Both Pearson correlations and partial correlations were examined for both samples. Fearful attachment was associated with self-reported depression severity for females with depression, but attachment styles were unrelated to depression severity for males with depression (study 1). For female university students, both fearful and preoccupied attachment styles were significantly related to depression severity, but again there were no associations for male university students when partial correlations were utilised (study 2). Study 3 revealed significantly higher levels of fearful attachment for women with depression compared with female university students and no significant differences for males overall. CONCLUSIONS: For females, the avoidance of intimacy for fear of rejection was associated with a broad spectrum of depressive symptoms. Results highlight gender differences in depressive experiences, and have implications for research into the effects of fearful attachment on social support and the therapeutic alliance. Limitations to this study include the reliance on self-report measures of attachment and depression severity, the small number of male participants in the clinical sample, and the use of a cross-sectional rather than longitudinal design.

Adult↗

Loss of alveolar attachments in smokers. A morphometric correlate of lung function impairment.

We studied post-mortem 9 nonsmokers' lungs and 9 smokers' lungs as well as 14 surgical smokers' lungs to examine the possible relationship of the number of alveolar attachments with airways inflammation and with lung function. Alveolar attachments are the alveolar walls radially attached to the small airways, and any discontinuity or rupture of these alveolar walls was considered abnormal. Normal and abnormal attachments were counted in nonsmokers and smokers and expressed as number of attachments, distance between attachments, and percentage of abnormal attachments. Although internal small airways diameter and mean linear intercept were not significantly different between smokers of either group and nonsmokers, significant differences in number of attachments (p less than 0.001), distance between attachments (p less than 0.01), and percentage of abnormal attachments (p less than 0.01) were found. The 3 indexes of alveolar attachments correlated significantly with the score for airways inflammation and with the elastic recoil pressure in smokers. No significant correlation with any other lung function test was found. We conclude that smokers have fewer alveolar attachments than do nonsmokers, and that the loss of alveolar attachments represents an early stage in the destruction of lung parenchyma, and is probably linked to inflammation of the small airways. Because of the strategic situation of this lesion, it could be responsible in part for the loss of elastic recoil seen in the initial stages of chronic obstructive pulmonary disease.

Aged↗

Attachment characteristics of bovine bronchial epithelial cells to extracellular matrix components.

Attachment of cells to extracellular matrix (ECM) plays an important role in the regulation of cell growth and differentiated function. We hypothesized that bronchial epithelial cells preferentially attach to ECM proteins and utilize specific receptors for ECM proteins. Bronchial epithelial cells were obtained from bovine lung by protease digestion. Both freshly isolated and cultured bronchial epithelial cells were plated onto plastic petri dishes coated with bovine serum albumin, type I collagen, type IV collagen, fibronectin, laminin, ECM synthesized by cultured bronchial epithelial cells, or uncoated. Freshly isolated cells demonstrated significant attachment to ECM but weak attachment to other matrix proteins. Cultured bronchial epithelial cells attached well to ECM; however, they had relatively increased attachment to type I collagen, type IV collagen, fibronectin, and laminin compared to freshly isolated cells. To determine whether the attachment of bronchial epithelial cells is arginine-glycine-aspartic acid (RGD)-mediated, an RGD-containing peptide known to block attachment mediated by many integrin receptors was added to the media (400 micrograms/ml). There was no inhibition of attachment of freshly isolated cells; however, there was significant but not complete inhibition of the attachment of the cultured cells to type IV collagen, laminin, and fibronectin, but not to type I collagen or ECM. Thus, freshly isolated bronchial epithelial cells readily adhere to ECM, and the attachment does not appear to be mediated by RGD-dependent receptors. Cultured bronchial epithelial cells demonstrate increased attachment to component proteins of ECM, and this attachment is, in part, to RGD-dependent receptors.

Animals↗

Cell attachment activity of cementum proteins and mechanism of endotoxin inhibition.

Cementum occupies a unique anatomical location where soft connective tissues of the periodontium are attached to root surfaces. Cell attachment properties of proteins present in cementum were studied. Human and bovine cementum were extracted with 0.5 mol/L CH3COOH followed by 4 mol/L guanidine, and proteins were separated by ion-exchange chromatography and SDS-polyacrylamide gel electrophoresis. Cells were labeled with radioactive amino acids and added to tissue-culture plastic plates incubated with cementum proteins, and attachment was measured. Results showed that cementum proteins promoted the attachment of smooth muscle cells, endothelial cells, and fibroblasts, but not epithelial cells. Fibroblasts attached more efficiently than other cell types, and they manifested spreading with re-organization of actin filaments. No attachment occurred to plates incubated with endotoxin from A. actinomycetemcomitans. Fewer fibroblasts attached to plates treated with cementum proteins in the presence of endotoxin, but cells pre-treated with endotoxin attached normally. Attachment was not inhibited when plates were incubated first with attachment proteins and then with endotoxin; however, it was decreased when endotoxin or bovine serum albumin preceded cementum proteins. Cementum proteins with Mr 68,000, 61,000, 55,000, and 36,000 (p68, p61, p55, and p36, respectively) manifested attachment activity, while protein(s) with Mr 23,000-24,000 did not. Western blots revealed that guanidine extracts contained three bands cross-reacting with anti-bovine sialoprotein-II antibody, but the p61, p55, and p36 were negative. We conclude that cementum contains bovine sialoprotein-II and at least four other fibroblast attachment proteins, and that they do not support epithelial cell attachment.(ABSTRACT TRUNCATED AT 250 WORDS)

Actinobacillus↗

Attachment of shiga toxigenic Escherichia coli to beef muscle and adipose tissue.

Shiga toxigenic Escherichia coli (STEC) serotypes are important foodborne pathogens that cause gastrointestinal disease worldwide. An understanding of how STEC strains attach to surfaces may provide insight into the potential persistence of and contamination with STEC in food environments. The initial attachment of a selection of STEC serotypes to beef muscle and adipose tissue was evaluated for isolates grown in planktonic and sessile culture. Initial experiments were performed to determine whether attachment differed among STEC strains and between the two modes of growth. Viable counts were obtained for loosely and strongly attached cells, and the strength of attachment (Sr) was calculated. All bacterial isolates grown in sessile culture attached in higher numbers to muscle and adipose tissue than did bacteria in planktonic cultures. For all attachment assays performed, mean concentrations for loosely attached cells were consistently higher than concentrations for strongly attached cells. The mean concentrations for strongly attached bacteria for planktonic and sessile cultures were significantly higher (P < 0.05) on adipose than on muscle tissue. However, some strains of STEC, particularly those from sessile culture, did not differ in their attachment to muscle or adipose tissue. Sr values were not significantly different (P > 0.05) among STEC isolates for all assays. No correlation was found between bacterial hydrophobicity and surface charge values (previously determined) and production of surface structures, viable counts, and Sr values. STEC grown in planktonic and sessile culture seems to behave differently with respect to attachment to muscle and adipose tissue. Cells in sessile culture may have a greater potential to strongly attach to meat surfaces.

Adipose Tissue↗