Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “AEROMONAS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 253 records · Page 14Linked to original sources
PubMed1990

Human serum antibody response to the presence of Aeromonas spp. in the intestinal tract.

A bacterial agglutination assay, a toxin-neutralizing assay, and an enzyme-linked immunosorbent assay (ELISA) were used to compare antibodies against intestinal Aeromonas strains in serum samples from healthy carriers (n = 6), from patients with acute (n = 15) or chronic (n = 8) gastroenteritis, from patients with gastroenteritis caused by other enteropathogenic bacteria (n = 3), and from healthy blood donors (n = 50). Evaluation of the bacterial agglutination assay showed that it was not very useful. The sensitivity of the ELISA in patients with acute or chronic aeromonas-associated diarrhea was 30% (7 of 23 patients were positive), whereas the specificity was 74% (13 of 50 healthy donors were positive). Positive results in the ELISA correlated with immunoglobulin M and immunoglobulin G responses to lipopolysaccharides of homologous Aeromonas strains, as determined by gel immunoradioassay and Western immunoblot analysis. The sera showed cross-reactions with heterologous Aeromonas strains and with Escherichia coli strains. The toxin-neutralizing assay was positive in 5 of 11 patients who had developed acute severe diarrhea associated with cytotoxin-producing Aeromonas strains (46% sensitivity), whereas only 3 of 50 healthy donors had low serum titers of cytotoxin-neutralizing antibodies (94% specificity). All five patients were over 60 years of age. Cytotoxin-neutralizing activity was not observed in the sera of other groups of patients with aeromonads in their feces. We concluded that the three different serologic assays were not consonant with one another and that only the toxin-neutralizing assay distinguished patients with acute diarrhea from other groups of patients.

Adolescent↗
PubMed1991

Aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens.

Previous DNA hybridization studies established 12 Aeromonas genospecies, from which nine phenotypic species have been proposed: Aeromonas hydrophila, A. sobria, A. caviae, A. media, A. veronii, A. schubertii, A. salmonicida, A. eucrenophila, and A. jandaei. We have delineated a new Aeromonas genospecies, A. trota, on the basis of 13 strains isolated primarily from fecal specimens from southern and southeastern Asia. All strains were highly related to the proposed type strain, AH2 (ATCC 49657T): 51 to 100% (60 degrees C) and 49 to 99% (75 degrees C), with 0.2 to 2.2 divergence. AH2 was only 16 to 41% (60 degrees C) related to all other Aeromonas type strains and DNA group definition strains. The unique profile of A. trota includes negative reactions for esculin hydrolysis, arabinose fermentation, and the Voges-Proskauer test, positive reactions for cellobiose fermentation, lysine decarboxylation, and citrate utilization, and susceptibility to ampicillin, as determined by the broth microdilution MIC method and the Bauer-Kirby disk diffusion method (10 micrograms). Nine of the A. trota strains were from a single study of 165 geographically diverse aeromonads. This finding questions the efficacy of screening fecal specimens for Aeromonas spp. with ampicillin-containing media and suggests a previously unrecognized prevalence of this new species.

Aeromonas↗
PubMed1996

Expression of the chitinase III gene of Aeromonas sp. no. 10S-24 in Escherichia coli.

The chitinase III gene of Aeromonas sp. No. 10S-24 was expressed in Escherichia coli. Production of chitinase III by E. coli was 3-fold higher than that of chitinases in the culture supernatant of Aeromonas sp. The enzyme from E. coli was purified and characterized. The molecular weight of the chitinase III from E. coli was estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to be 55,000. This agreed with the calculated molecular weight of the mature chitinase III (54,111). However, it was different from that of the enzyme from Aeromonas sp. It showed that the chitinase III from Aeromonas sp. had an additional sugar chain, causing the higher molecular weight. Chitinase III from E. coli had almost the same enzymatic properties as chitinase III from Aeromonas sp., but the specific activity of the chitinase III from E. coli is slightly higher than that of the native chitinase III. Both enzymes hydrolyzed chitosan (80% deacetylated) well.

Aeromonas↗
PubMedDate not supplied

Presence of Aeromonas spp in water from drinking-water- and wastewater-treatment plants in Mexico City.

The frequency of Aeromonas spp in three wastewater-treatment plants (WWTPs) and two drinking-water plants (DWPs) in México City was determined. Samples were taken throughout a year by the Moore's swab technique. A total of 144 samples were obtained from WWTPs and 96 from DWPs of both incoming and outflowing water. Aeromonas spp was isolated in 31% of the samples, from both kinds of sources. The technique used for the isolation of the pathogen was suitable for samples with high associate microbiota content and for those with a scarce microbial content. The presence of mesophilic-aerobic, coliform, and fecal-coliform organisms was investigated to determine whether there was any correlation with the presence of Aeromonas spp. Most samples from WWTP, which did not comply with the Mexican standards, had the pathogen, and some of the samples from the outflow of the DWP, which were within the limits set by the Mexican standards, also had Aeromonas spp. Most samples containing Aeromonas spp. had concentrations below 0.1 ppm residual chlorine, and the strains were resistant to 0.3 ppm, which supports the recommendation to increase the residual chlorine concentration to 0.5 to 1.0 ppm, as recommended by the Mexican standards.

Aeromonas↗
PubMed1999

Prevalence and characteristics of Aeromonas species isolated from processed channel catfish.

From August 1994 to May 1995, 238 channel catfish fillets collected from three processing plants in the Mississippi Delta at four time periods were tested for the presence of Aeromonas species. Identification of Aeromonas spp. was accomplished using an automated Vitek bioassay system with gram-negative and nonfermenter cards. Approximately 36.1% were positive for A. hydrophila, 35.7% for A. sobria, and 10.9% for A. caviae. All three Aeromonas spp. were found in all three processing plants, and the incidence of A. hydrophila contamination appeared to be higher in summer than other seasons. Eighty-six percent of the Aeromonas isolates were hemolytic on 5% sheep blood agar plates. Most isolates were susceptible to chloramphenicol, neomycin, streptomycin, and trimethoprim-sulfamethoxazole and resistant to ampicillin and bacitracin. Results suggest that Aeromonas spp. are prevalent in processed channel catfish, and most isolates are hemolytic and resistant to ampicillin and bacitracin.

Aeromonas↗
PubMed1995

An alternative bacteriological medium for the isolation of Aeromonas spp.

Two solid bacteriologic media were compared for cultivating Aeromonas spp. from piscine sources: the Rimler-Shotts (RS) medium and a starch-glutamate-ampicillin-penicillin-based medium (SGAP-10C) used for the recovery of Aeromonas spp. from water samples. The selective and differential capacities of the media were assessed March through October 1992 by recovery rate and phenotype of 99 isolates representing 15 genera of bacteria. Recovery frequency of Aeromonas spp. (n = 62) was similar at 97% on RS and 95% on SGAP-10C. The SGAP-10C medium proved to be more specific than RS toward Aeromonas species (P < or = 0.005). Use of SGAP-10C at 24 C for 48 hr offers a better choice for the laboratory recovery of Aeromonas spp. from clinical fish specimens.

Aeromonas↗
PubMed2001

[Aeromonas species infection with severe clinical manifestation in okinawa, Japan -association with gas gangrene-].

We experienced two patients having Aeromonas species infection with severe clinical manifestations. The one patient was a 15-year-old high school girl student, who had been healthy in her school life, was admitted to the hospital with a sudden onset of left thigh muscle pain and swelling. She subsequently went into septic shock and died one day after admission. Pathological examination on autopsy revealed massive gas formation, skin bullas and ulcers, and extensive severe soft tissue damage throughout the body. Also, all the specimens, including brain, liver, spleen, thigh muscle, and blood in cardiac cavity, were positive for A. veronii biovar sobria. The other patient was 35-year-old man, who suffered from multiple bone fractures during the work in the harbor. One day after admission, he became febrile and went into septic shock. With the presumptive diagnosis of sepsis and gas gangrene, amputation of left thigh was performed. The exudate and aspirate of the amputated portion were repeatedly positive for A. hydrophila. Through the surveillance in Okinawa, Kagoshima, Miyazaki, and Kumamoto Prefectures, a total of 426 isolates from blood cultures were collected in the period from August, 1999 to February, 2000. Of these, 14 isolates (3.3%) were the species of Aeromonas. Of 14 isolates of Aeromonas, 13 were reported from Okinawa and the remaining one was from Kumamoto. Most patients had underlying diseases, particularly liver diseases including liver cirrhosis. The mortality rate was extremely high at 62.5%, and the patients died in short terms after blood culture became positive. With these, Aeromonas species infection is unique to Okinawa, and positive blood culture for Aeromonas species potentially indicates a high-risk, particularly among the patients with underlying diseases.

Adolescent↗
PubMedDate not supplied

Direct detection of diarrheagenic Aeromonas from faeces by polymerase chain reaction (PCR) targeting aerolysin toxin gene.

Detection of diarrheagenic Aeromonas specific aerolysin toxin (Aer) gene by PCR based assay and isolation, identification of diarrhea causing Aeromonas from faeces by culture methods were carried out in the Division of Active Surveillance, National Institute of Cholera and Enteric Diseases (NICED), Kolkata, India for a period of 12 months. Out of 602 faecal samples collected from patients with acute diarrhea admitted in Infectious Diseases (ID) Hospital, Kolkata, 68 (11.29%) samples were found to be possessing Aer gene by PCR technique. The conventional culture methods using selective media yielded only 64 (10.6%) Aeromonas strains from the same faecal samples. The different Aeromonas species possessing Aer gene identified by PCR based technique include A. hydrophila (55.8%), A. caviae (17.6%), A. veronii (10.2%), A. schubertii (4.4%), A. jandaei (2.9%) and A. trota (8.8%). The isolation and identification of Aeromonas by routine culture did not detect enterotoxigenic A. trota present in four diarrheal faecal samples. The failure of the growth of enterotoxigenic A. trota on selective media may be attributed to the ampicillin susceptibility of those strains. The quality control studies revealed that PCR method for the direct detection of Aer gene from the faeces has the sensitivity of 100% and specificity of 98%.

Adolescent↗
PubMed2004

Phenotypic and genotypic identification of Aeromonas spp. isolated from a chlorinated intermittent water distribution system in Lebanon.

Aeromonas spp. were detected in samples collected from both untreated groundwater and treated drinking water in Lebanon. Aeromonas spp. levels ranged between 2 and 1,100 colonies per 100 ml in the intake underground well and between 3 and 43 colonies per 100 ml in samples from the distribution system. Samples positive for Aeromonas spp. from the network had a free chlorine level ranging between 0 and 0.4 mg l(-1). Multiple antibiotic-resistance was common among the isolated aeromonads; all were resistant to amoxycillin while 92% showed resistance to cephalexin. Haemolysis on blood agar was detected in 52% of the isolates recovered from the distribution network and 81% of isolates from the untreated underground source. The Biolog microbial identification system assigned identities to all of the isolated presumptive aeromonads (at least at the genus level), which was not the case with the API 20NE strips. Differences at the species level were observed when results from the Biolog system were compared with identification based on the MicroSeq 500 16S rDNA sequence analysis. The presence of Aeromonas spp. in drinking water can be an important threat to public health, thus greater awareness of Aeromonas strains as potential enteropathogens is warranted.

Aeromonas↗
PubMed1991

Significance of sinking particles in the distribution of motile Aeromonas during the winter season.

The role of sinking particles in the distribution of motile Aeromonas species was studied during the winter season. Various environmental parameters and microbial populations were investigated to elucidate the relationship with motile aeromonads. Motile Aeromonas species were enumerated by most probable number technique with alkaline peptone water as the enrichment medium and modified pril-xylose ampicillin agar as the plating medium. Aeromonas species were isolated in a water column in any one of the two procedures but sediment and plankton samples exhibited an irregular isolation pattern for these organisms. Aeoromonas species were continuously isolated in sinking particles with the highest counts during January. Of the 206 isolates identified, three known motile Aeromonas species were observed of which A. caviae accounted for 51.4% of the total. Toxin characterization showed that 20% of the strains produced haemolysin as well as cytotoxin, and A. hydrophila was highly toxigenic. Statistical analyses revealed that nutrients govern the distribution of Aeromonas. It may be that riverine discharge influences the distribution of motile aeromonads in this environment.

Aeromonas↗
PubMed1990

[Aeromonas species as cause of diarrhea and infections outside the gastrointestinal tract in The Netherlands].

In a national survey in the period May 1986-December 1987, Aeromonas was isolated from 277 out of 16,857 (1.6%) samples of watery, bloody or mucous stool, from patients with diarrhoea. There was a clear seasonal pattern (less than 1% in winter, up to 3% in summer). A. caviae was isolated most frequently (49%), followed by A. sobria (35%) and A. hydrophila (15%). Some non-identifiable strains were isolated as well. Aeromonas were isolated in particular from faeces of patients aged over 70 years (predominantly A. sobria) or under 5 years (predominantly A. caviae). In 67% Aeromonas was isolated as the only possible bacterial cause of diarrhoea, but in 33% other enteropathogenic bacteria were found as well (17% Campylobacter, 14% Salmonella, 2% Shigella). In addition, all Aeromonas isolates were collected which were obtained in normal diagnostic activities in the participating laboratories, among others from blood, from pus or wound fluid, from faecal samples which did not meet the above mentioned criteria. A. caviae was the dominant species in 'other' faeces and 'various' body sites but was not isolated from blood. The results of this study do not indicate that routine examinations for Aeromonas in faeces of patients with diarrhoea are necessary.

Adolescent↗
PubMedDate not supplied

[Are Aeromonas species a cause of diarrheal diseases in Yugoslavia?].

Isolation and identification of Aeromonas spp. have been performed in 1,610 stools in the period from January 1987 to April 1988. Of 1300 stool specimens taken from the patients with diarrheal syndrome (540 stools of children and 760 stools of adults) Aeromonas spp. were isolated only in 4 patients (0.30%, P less than 0.001). Positive stools were found in 3 adult patients and one child. In 310 examined stools of healthy adult persons Aeromonas spp. were not isolated. A significantly higher incidence of Aeromonas species has been observed in different kind of water. They were isolated in 58.3% in waste water, in 56.2% in river water and in 22.2% in well water. The results have shown that Aeromonas species are not important causative agents of diarrheal syndrome in our country but their importance for public health regarding their high distribution in the nature necessitate their further studies.

Aeromonas↗
PubMed1988

[The occurrence of antibiotic-resistant Aeromonas and Vibrio species in river water].

262 samples of river water had been analysed for the occurrence of antibiotic resistant Aeromonas and Vibrio strains. The bacteria were cultured on selective media containing the antimicrobial chemotherapeutic agents oxytetracycline, chloramphenicol, streptomycin, ampicillin, kanamycin, gentamycin or trimethoprim. Although it was not possible to identify antibiotic resistant bacteria of the genus Vibrio, resistant strains of Aeromonas hydrophila with a wide variety of resistance patterns could be cultured in many cases. R plasmids were found by means of conjugation in 21.2% of the 826 tested Aeromonas hydrophila strains. They were characterized with the aid of the fin- and pili test. The majority of the R plasmids were fin-negative, and the sex pili could be assigned strikingly often to sex pilus group C with the phages we used. 15 strains of Aeromonas hydrophila had been tested in agarose gel electrophoresis on plasmid DNA. In 11 Aeromonas hydrophila strains with transferable and in 3 from 4 strains with non transferable multiple antibiotic resistance plasmid DNA with 100 Megadalton had been found.

Aeromonas↗
PubMed1986

[Diarrhea caused by Aeromonas in Ivory Coast].

The authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with Aeromonas in Ivory-Coast. The results relate to a study realized from January 1982 to July 1985. Out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. Aeromonas caviae is the most frequent species (50%) compared to 27.3% for Aeromonas sobria, and 22.7% for Aeromonas hydrophila.

Adolescent↗
PubMed1980

[Studies of lipase and antilipase on different strains of the genera Serratia, Aeromonas and Vibrio (author's transl)].

Lipase and antilipase were studied in the following genera: Serratia, Aeromonas and Vibrio. Numerous comparative investigations showed that the Jipase of Serratia marcescens is not of a uniform antigenic structure as not all antilipase-sera of Serratia marcescens have the ability to neutralize the lipase of all studied strains of this species. Cross-reactions were seen between the genera Serratia and Aeromonas. A special reaction of the antilipase sera of Aeromonas hydrophila and Aeromonas punctata could be demonstrated on the lipase of one strain of Serratia marcescens. Those Aeromonas sera could neutralize the lipase of one strain of Serratia marcescens.

Aeromonas↗
PubMed1980

Enterotoxigenicity of aeromonas strains in suckling mice.

The enterotoxigenicity of 170 Aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. The strains were grown on Syncaye culture medium covered with sterilized membrane for Kiil-kidney. The culture supernatants were inoculated orally. Ileal loop dilatation was compared to that produced by the international standard enterotoxic Escherichia coli B7A (O148 : H28) and B2C (O6 : H16) strains. Of the 87 Aeromonas hydrophila strains 69, of the 76 Aeromonas punctate subsp. caviae strains 9, the 6 Aeromonas punctata subsp. punctata strains 5, and 1 Aeromonas salmonicida subsp. achromogenes gave a positive reaction in the test.

Aeromonas↗
PubMed1994

Oral challenge with Aeromonas in protein-malnourished mice.

The lack of an animal model for Aeromonas-associated diarrhoea has hindered progress toward understanding the pathogenesis of this potentially important enteric infection. Protein-malnourished mice were challenged orally with Aeromonas strains to determine if diminished levels of resistance would allow the induction of a diarrhoeal response. The 15 Aeromonas spp. faecal isolates used for challenge included 7 A. caviae, 4 A. hydrophila, 1 A. sobria bv. sobria, and 1 A. sobria bv. veronii from patients with diarrhoea, and 2 A. caviae from healthy volunteers. All had at least 1 known virulence marker, with the exception of 1 strain. Mice on the protein deficient diet had lost an average of 23% of their initial body weight at the time of challenge. Although mice consumed 10(8) cfu per day for a minimum of 4 days, none became ill due to Aeromonas spp. ingestion. Aeromonas spp. were isolated from 75% of faecal cultures obtained 7 days after initial challenge, indicating bowel colonization had occurred.

Aeromonas↗
PubMed1994

Aeromonas peritonitis.

Five new cases of peritonitis caused by Aeromonas species are reported, and 29 others described in the literature are reviewed. Males predominated (71%), and the mean age was 56.9 years. Acquisition was nosocomial in 20% of the cases. All patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [CAPD]), and 9% had an intestinal perforation. Symptoms were similar to those of peritonitis caused by other pathogens, with the exception of diarrhea, which occurred in 25% of cases. Blood cultures were positive in 74% of the cases. The species isolated were Aeromonas hydrophila (27), Aeromonas sobria (5), and Aeromonas caviae (2). The overall case-fatality rate was 57%. Three strains were resistant to cotrimoxazole. Aeromonas species should be taken into account as a cause of peritonitis in patients with cirrhosis or who are undergoing CAPD.

Adolescent↗