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[Maintaining mechanism of species diversity of land plant communities].

The maintaining mechanism of species diversity of land plant communities is a key and advancing edge in biodiversity study. Botanists and ecologists have presented many hypotheses and theories with controversies, and no general theory system was available. In this paper, the problem was reviewed mainly on two scales. The first was big spatial scale, aiming at the physical and natural factors that affect the species diversity, including histories and ages of plant communities, gradient changes such as latitude gradient, water gradient, altitude gradient and soil nutrients gradient, area effect, and isolation; and the second was concentrated on a special plant community, and mainly discussed the relationships of biodiversity with biotic factors (primary productivity, relationship between species, and gap dynamics) and abiotic factors (succession, disturbance and spatial heterogeneity, and human activity).

Biodiversity↗

A field experimental study of lignin sand stabilizing material (LSSM) extracted from spent-liquor of straw pulping paper mills.

A new technique was introduced for sand stabilization and re-vegetation by use of lignin sand stabilizing material (LSSM). LSSM is a reconstructed organic compound with lignin as the most dominant component from the extracts of black-liquor issued by straw pulp paper mills. Unlike the polyvinyl acetate or foamed asphalt commonly used for dune stabilization, the new material is plant-friendly and can be used with virescence actions simultaneously. The field experimental study was conducted since 2001 in China's Northwest Ningxia Hui Autonomous Region and has been proved that LSSM is effective in stabilizing the fugitive dunes, making the arenaceous plants survive and the bare dune vegetative. The advisable solution concentration is 2% and the optimal field spraying quantity is 2.5 L/m2. The soil nutrients of the stabilized and greened dune, such as organic matter, available phosphorous and total nitrogen are all increased compared with the control treatment, which is certainly helpful to the growth of arenaceous plants. The technique is worthwhile to be popularized because it is provided not only a new method for desertification control but also an outlet for cleaning contaminants issued from the straw paper mills.

Lignin↗

Seasonal variation of nitrogen-concentration in the surface water and its relationship with land use in a catchment of northern China.

Surface waters can be contaminated by human activities in two ways: (1) by point sources, such as sewage treatment discharge and storm-water runoff; and (2) by non-point sources, such as runoff from urban and agricultural areas. With point-source pollution effectively controlled, non-point source pollution has become the most important environmental concern in the world. The formation of non-point source pollution is related to both the sources such as soil nutrient, the amount of fertilizer and pesticide applied, the amount of refuse, and the spatial complex combination of land uses within a heterogeneous landscape. Land-use change, dominated by human activities, has a significant impact on water resources and quality. In this study, fifteen surface water monitoring points in the Yuqiao Reservoir Basin, Zunhua, Hebei Province, northern China, were chosen to study the seasonal variation of nitrogen concentration in the surface water. Water samples were collected in low-flow period (June), high-flow period (July) and mean-flow period (October) from 1999 to 2000. The results indicated that the seasonal variation of nitrogen concentration in the surface water among the fifteen monitoring points in the rainfall-rich year is more complex than that in the rainfall-deficit year. It was found that the land use, the characteristics of the surface river system, rainfall, and human activities play an important role in the seasonal variation of N-concentration in surface water.

China↗

Surface N balances and reactive N loss to the environment from global intensive agricultural production systems for the period 1970-2030.

Abstract Data for the historical years 1970 and 1995 and the FAO-Agriculture Towards 2030 projection are used to calculate N inputs (N fertilizer, animal manure, biological N fixation and atmospheric deposition) and the N export from the field in harvested crops and grass and grass consumption by grazing animals. In most industrialized countries we see a gradual increase of the overall N recovery of the intensive agricultural production systems over the whole 1970-2030 period. In contrast, low N input systems in many developing countries sustained low crop yields for many years but at the cost of soil fertility by depleting soil nutrient pools. In most developing countries the N recovery will increase in the coming decades by increasing efficiencies of N use in both crop and livestock production systems. The surface balance surplus of N is lost from the agricultural system via different pathways, including NH3 volatilization, denitrification, N20 and NO emissions, and nitrate leaching from the root zone. Global NH3-N emissions from fertilizer and animal manure application and stored manure increased from 18 to 34 Tg x yr(-1) between 1970 and 1995, and will further increase to 44 Tg x yr(-1) in 2030. Similar developments are seen for N2O-N (2.0 Tg x yr(-1) in 1970, 2.7 Tg x yr(-1) in 1995 and 3.5 Tg x yr(-1) in 2030) and NO-N emissions (1.1 Tg x yr(-1) in 1970, 1.5 Tg x yr(-1) in 1995 and 2.0 Tg x yr(-1) in 2030).

Agriculture↗

[Adaptation strategies of seed germination and seedling growth to sand dune environment].

Sand dune plants possess many adaptation strategies to withstand sand environment, e. g., some desert plant seeds are dormant when matured, which helps them to escape from unfeasible environment conditions and be preserved as seed bank. The seed germination and seedling establishment of psammophytes need moderate sand burial, while excessive burial will inhibit seed germination and seedling emergence. Seeds without germination in deeper sand are in enforced dormancy, and form soil seed bank. Sand dune plant seedlings could tolerant finite sand burial by increasing the number of nodes per culm and elongating internodes. When the seedlings are partially buried, they could survive through the maintenance of photosynthesis organism. Once sand burial exceed the threshold of the plant, seedlings growth will be restrained and the growth ability even permanently lost. Other factors such as salt spray, insect herbivory, and lack of soil nutrients also affect seed germination and seedling establishment. The precipitation in desert and sand land is unpredictable and irregular. Sand erosion leads roots be exposed to the air and dehydrated to die. However, seedlings of some desert plant have the ability to tolerate desiccation for a period of time after germination. Once there is rain, the seedlings will rivive.

Adaptation, Physiological↗

The influence of a neotropical herbivore (Lamponius portoricensis) on nutrient cycling and soil processes.

The role of phytophagous insects in ecosystem nutrient cycling remains poorly understood. By altering the flow of litterfall nutrients from the canopy to the forest floor, herbivores may influence key ecosystem processes. We manipulated levels of herbivory in a lower montane tropical rainforest of Puerto Rico using the common herbivore, Lamponius portoricensis (Phasmatidea), on a prevalent understory plant, Piper glabrescens (Piperaceae), and measured the effects on nutrient input to the forest floor and on rates of litter decomposition. Four treatment levels of herbivory generated a full range of leaf area removal, from plants experiencing no herbivory to plants that were completely defoliated (>4,000 cm(2) leaf area removed during the 76-day study duration). A significant (P<0.05) positive regression was found between all measures of herbivory (total leaf area removed, greenfall production, and frass-related inputs) and the concentration of NO (3) (-) in ion exchange resin bags located in the litter layer. No significant relationship was found between any of the herbivory components and resin bag concentrations of NH (4) (+) or PO (4) (-) . Rates of litter decay were significantly affected by frass-related herbivore inputs. A marginally significant negative relationship was also found between the litter mass remaining at 47 days and total leaf area removed. This study demonstrated a modest, but direct relationship between herbivory and both litter decomposition and NO (3) (-) transfer to the forest floor. These results suggest that insect herbivores can influence forest floor nutrient dynamics and thus merit further consideration in discussions on ecosystem nutrient dynamics.

Animals↗

Impact of genetically modified crops and their management on soil microbially mediated plant nutrient transformations.

One of the potential environmental effects of the recent rapid increase in the global agricultural area cultivated with transgenic crops is a change in soil microbially mediated processes and functions. Among the many essential functions of soil biota are soil organic matter decomposition, nutrient mineralization and immobilization, oxidation-reduction reactions, biological N fixation, and solubilization. However, relatively little research has examined the direct and indirect effects of transgenic crops and their management on microbially mediated nutrient transformations in soils. The objectives of this paper are to review the available literature related to the environmental effects of transgenic crops and their management on soil microbially mediated nutrient transformations, and to consider soil properties and climatic factors that may affect the impact of transgenic crops on these processes. Targeted genetic traits for improved plant nutrition include greater plant tolerance to low Fe availability in alkaline soils, enhanced acquisition of soil inorganic and organic P, and increased assimilation of soil N. Among the potential direct effects of transgenic crops and their management are changes in soil microbial activity due to differences in the amount and composition of root exudates, changes in microbial functions resulting from gene transfer from the transgenic crop, and alteration in microbial populations because of the effects of management practices for transgenic crops, such as pesticide applications, tillage, and application of inorganic and organic fertilizer sources. Possible indirect effects of transgenic crops, including changes in the fate of transgenic crop residues and alterations in land use and rates of soil erosion, deserve further study. Despite widespread public concern, no conclusive evidence has yet been presented that currently released transgenic crops, including both herbicide and pest resistant crops, are causing significant direct effects on stimulating or suppressing soil nutrient transformations in field environments. Further consideration of the effects of a wide range of soil properties, including the amount of clay and its mineralogy, pH, soil structure, and soil organic matter, and variations in climatic conditions, under which transgenic crops may be grown, is needed in evaluating the impact of transgenic crops on soil nutrient transformations. Future environmental evaluation of the impact of the diverse transgenic crops under development could lead to an improved understanding of soil biological functions and processes.

Agriculture↗

Synthesis of HNO3 from organic matter and its influence on nutrient replenishment in forest soils.

Chemical analyses during a decade of bulk precipitation, throughfall, humus water and soil water in forest plots ranging from sand to silt of Pleistocene origin in Flanders (N-Belgium) prove that previous and present weathering is predominantly due to synthesis of HNO3 from soil organic matter. The HNO3 reacts with silicates and possibly PO4(3-) species, releasing Al, Fe, nutrient base cations and H2PO4-, and is transformed into NO3-. In all soils solubilized Al3+ is predominantly associated with NO3- and with some organic bases in the coarse-textured soils with undisturbed or previously plowed spodic B horizon. The amounts of ions leached, especially Al3+ and NO3-, are much higher in the sandy than in the silty soils as a result of a stronger neutralization of acidity in the silty soils. Nutrients, leached from the soil, have to be replenished in some way for a sustained forest growth. N- and S-species must come from the atmosphere. Basic cations and P-species have to be supplied out of the solid phase. The concentrations of NO3- in the soil water show that the liberation of these elements from the solid phase is performed by HNO3. Especially in the coarse-textured soils the need for NO3- is high. Therefore, instead of being a real hazard for the forest ecological system, supplementary addition of HNO3 or NH3 can be beneficial.

Belgium↗

Intraspecific litter diversity and nitrogen deposition affect nutrient dynamics and soil respiration.

Anthropogenic forces are concurrently reducing biodiversity and altering terrestrial nutrient cycles. As natural populations decline, genetic diversity within single species also declines. The consequences of intraspecific genetic loss for ecosystem functions are poorly understood, and interactions among intraspecific diversity, nitrogen deposition, and nutrient cycling are unknown. We present results from an experiment that simulated both a decline in biodiversity and an increase in nitrogen deposition. In soil microcosms, we tested effects of variation in intraspecific litter diversity and nitrogen deposition on soil respiration and nitrogen leaching. Increases in intraspecific litter diversity increased soil respiration overall, with the greatest increases in respiration occurring under high nitrogen deposition. Nitrogen deposition increased the amount of inorganic nitrogen leached, while the amount of dissolved organic nitrogen leached was correlated with initial litter chemistry (lignin concentration) and remained independent of litter diversity and nitrogen deposition treatments. Our results demonstrate the potential for losses in genetic diversity to interact with other global environmental changes to influence terrestrial nutrient cycles.

Ecosystem↗

Measurement of nutrients in green house soil with laser induced breakdown spectroscopy.

Laser-induced breakdown spectroscopy (LIBS) has been applied for the determination of nutrients in the green house soil samples. We determined appropriate spectral signatures of vital nutrients and calibrated the method to measure the nutrients in a naturally fertilized plot, cultivated with tomato and cucumber plants. From the calibration curves we predicted the concentrations of important nutrients such as Ca, K, P, Mg, Fe, S, Ni and Ba in the soil. Our measurements proved that the LIBS method rapidly and efficiently measures soil nutrients with excellent detection limits of 12, 9, 7, 9, 7, 10, 8 and 12 mg/kg for Ca, K, P, Mg, Fe, S, Ni and Ba respectively with a precision of approximately 2%, The unique features of LIBS for rapid sample analysis demonstrated by this study suggests that this method offers promise for precision measurements of soil nutrients as compared to conventional methods in short span of time.

Barium↗

Assessment and treatment of hydrocarbon inundated soils using inorganic nutrient (N-P-K) supplements: II. A case study of eneka oil spillage in Niger Delta, Nigeria.

Polluted soils from Eneka oil field in the Niger delta region of Nigeria were collected two months after recorded incidence of oil spillage as part of a two-site reclamation programme. The soils were taken on the second day of reconnaissance from three replicate quadrats, at surface (0-15 cm) and subsurface (15-30 cm) depths, using the grid sampling technique. Total extractable hydrocarbon content (THC) of the polluted soils ranged from 1.006 x 10(3)-5.540 x 10(4) mg/kg at surface and subsurface depths (no overlap in Standard Errors at 95% Confidence Level). Greenhouse trials for possible reclamation were later carried out using (NH(4))(2)SO(4), KH(2)PO(4) and KCl (N-P-K) fertilizer as nutrient supplements. Nitrogen as NO(3)-N and potassium were optimally enhanced at 2% (w/w) and 3% (w/w) of the N-P-K supplementation respectively. Phosphorus, which was inherently more enhanced in the soils than the other nutrients, maintained same level impact after 20 g treatment with the N-P-K fertilizer. Total organic carbon (%TOC), total organic matter (%TOM), pH and % moisture content all provided evidence of enhanced mineralization in the fertilizer treated soils. If reclamation of the crude oil inundated soils is construed as the return to normal levels of metabolic activities of the soils, then the application of the inorganic fertilizers at such prescribed levels would duly accelerate the remediation process. This would be, however, limited to levels of pollution empirically defined by such THC values obtained in this study. The data on the molecular compositional changes of the total petroleum hydrocarbon content (TPH) of the spilled-oil showed the depletion of the fingerprints of the n-paraffins, nC(8)-nC(10), and complete disappearance of C(12)-C(17) as well as the acyclic isoprenoid, pristane, all of which provided substantial evidence of degradation.

Ammonium Sulfate↗

Growth of Indigenous Rhizobium leguminosarum and Rhizobium meliloti in Soils Amended with Organic Nutrients.

The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with different cropping histories. Four additional soils were screened with a glucose amendment. Nutrient amendments stimulated growth of indigenous rhizobia, allowing subsequent replication of indigenous bacteriophages. Phage populations were enumerated by plating soil extracts on 19 R. leguminosarum and 9 R. meliloti indicator strains, including root nodule isolates from the soils assayed. On the basis of indirect phage analysis, all soils contained native rhizobia similar to one or more of the indicator strains, although not all indicator strains were detected in soil. All organic compounds stimulated growth of indigenous rhizobia, but the growth response varied for each rhizobial strain depending on the nutrient, the nutrient concentration, and the soil. Indigenous rhizobia readily utilized most organic compounds except phenylalanine, glycine, and aspartic acid. The ability of indigenous rhizobia to utilize a wide range of organic compounds as growth substrates in situ indicates their ability to successfully compete with other soil bacteria for nutrients in these soils.

Journal Article↗

Assessment of wool waste and hair waste as soil amendment and nutrient source.

A field and two container experiments were conducted to assess uncomposted wool and hair wastes as a nutrient source for crops and to evaluate their potential to improve soil biological and chemical properties. Overall, addition of wool or hair waste to soil increased yields of basil (Ocimum basilicum L. 'Trakia'), thorn apple (Datura innoxia Mill. 'Inka'), peppermint (Mentha x piperita L. 'Black Mitchum'), and garden sage (Salvia officinalis L. 'Desislava'), increased NH(4)-N and NO(3)-N in soil, increased total N (and protein) content in plant tissue, stimulated soil microbial biomass, and decreased mycorrhizae colonization of plant roots of thorn apple but not in basil. Wool and hair waste additions to soil altered slightly the content and composition of plant secondary metabolites (essential oils or alkaloids); however, overall the constituents remained within the "typical" range for the respective crops. Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis demonstrated that wool and hair wastes decompose slowly under field or greenhouse conditions, and act as a slow release S, N, P, and K fertilizer. These results, along with the measured concentrations of NO(3)-N in soil at harvest, suggest that the addition of wool or hair waste of only 3.3 g kg(-1) of soil may support two to five harvests or crops under greenhouse conditions and two to four field seasons in field production systems, and would improve soil biological and chemical characteristics. Further research is needed to optimize the rate of application of these waste materials to the nutrient requirements of specific crops to avoid nitrate leaching into the ground water. In addition, the effect of wool and hair waste on other environmental end points should also be further investigated before specific recommendations for growers are provided.

Absorptiometry, Photon↗

Ring-testing and field-validation of a terrestrial model ecosystem (TME)--an instrument for testing potentially harmful substances: effects of carbendazim on nutrient cycling.

The effect of the fungicide carbendazim (applied in the formulation Derosal) on nutrient cycling in soil was determined in Terrestrial Model Ecosystem (TME) tests and corresponding field-validation studies, which were performed in four different countries (United Kingdom, Germany, Portugal, and The Netherlands). The tests used different soil types, and lasted for 16 weeks. On three of the four sites, grassland soils were used while the fourth site had an arable soil. TMEs consisted of intact soil columns (diameter 17.5 cm; length 40 cm) and were taken from the site where the field study was performed. In the first series of TME tests, carbendazim applied at dosages unto 77.8 kg a.i./ha did not affect sulfate and phosphate concentrations in the top 15 cm soil layers. These nutrients were therefore no longer included in the second series of TME tests and the field-validation studies. Ammonium concentrations in the top soil layers of TMEs and field plots, and in the leachates of the TME columns did not show any effect of carbendazim treatment. Nitrate concentrations in soils and leachates did show some reduction at the highest treatment levels (77.8 kg a.i./ha in the first TME tests, 87.5 kg a.i/ha in the second TME tests and the field-validation studies). Since nitrate concentrations in both soils and leachates were correlated with soil moisture content, these effects could however, mainly be attributed to variations in soil moisture contents, and in some cases also indirectly to effects on earthworm activity. It is concluded that carbendazim, even at dosages as high as 87.5 kg a.i./ha, does not have a significant impact on soil nutrient cycling processes. Nutrient levels in TME tests and the field-validation studies generally showed similar patterns, thus confirming the predictive value of the TME test system.

Benzimidazoles↗

Characteristics of wood ash and influence on soil properties and nutrient uptake: an overview.

Wood industries and power plants generate enormous quantities of wood ash. Disposal in landfills has been for long a common method for removal. New regulations for conserving the environment have raised the costs of landfill disposal and added to the difficulties for acquiring new sites for disposal. Over a few decades a number of studies have been carried out on the utilization of wood ashes in agriculture and forestry as an alternative method for disposal. Because of their properties and their influence on soil chemistry the utilization of wood ashes is particularly suited for the fertility management of tropical acid soils and forest soils. This review principally focuses on ash from the wood industry and power plants and considers its physical, chemical and mineralogical characteristics, its effect on soil properties, on the availability of nutrient elements and on the growth and chemical composition of crops and trees, as well as its impact on the environment.

Hydrogen-Ion Concentration↗

Nutrient amendments in soil DNA stable isotope probing experiments reduce the observed methanotroph diversity.

Stable isotope probing (SIP) can be used to analyze the active bacterial populations involved in a process by incorporating 13C-labeled substrate into cellular components such as DNA. Relatively long incubation times are often used with laboratory microcosms in order to incorporate sufficient 13C into the DNA of the target organisms. Addition of nutrients can be used to accelerate the processes. However, unnatural concentrations of nutrients may artificially change bacterial diversity and activity. In this study, methanotroph activity and diversity in soil was examined during the consumption of 13CH4 with three DNA-SIP experiments, using microcosms with natural field soil water conditions, the addition of water, and the addition of mineral salts solution. Methanotroph population diversity was studied by targeting 16S rRNA and pmoA genes. Clone library analyses, denaturing gradient gel electrophoresis fingerprinting, and pmoA microarray hybridization analyses were carried out. Most methanotroph diversity (type I and type II methanotrophs) was observed in non-amended SIP microcosms. Although this treatment probably best reflected the in situ environmental conditions, one major disadvantage of this incubation was that the incorporation of 13CH4 was slow and some cross-feeding of 13C occurred, thereby leading to labeling of nonmethanotroph microorganisms. Conversely, microcosms supplemented with mineral salts medium exhibited rapid consumption of 13CH4, resulting in the labeling of a less diverse population of only type I methanotrophs. DNA-SIP incubations using water-amended microcosms yielded faster incorporation of 13C into active methanotrophs while avoiding the cross-feeding of 13C.

Carbon Isotopes↗

Solute response to changing nutrient loads in soil and walled ceramic cup samplers under continuous extraction.

This report evaluates a vacuum-assisted walled percolation sampler preconditioned in soil, and examines the dynamic response of leachate solutes. The 20-cm walled percolation sampler extracted soil water under continuous tension via a ceramic cup collector embedded in a silica flour layer, whose upper surface interfaced with field soil. In the laboratory, alternating solutions with high and low NO3-N (232 or 3.6 mg L(-1)), molybdate-reactive P (MRP) (1.75 or 0.0 mg L(-1)), K+ (568 or 3.6 mg L(-1)), and Br- (9.6 or 0.0 mg L(-1)) concentrations were delivered directly to the (i) sampler ceramic cup; (ii) silica flour bed surface, or (iii) 12-mm soil layer placed over the silica flour bed. For alternating input solutions delivered to the silica-flour bed surface, (i) solute breakthrough (95% equivalency) occurred in 4 pore volumes and was the same for both the high and low concentration input phases of the application, and (ii) concentrations of NO3-N, Br-, and MRP in cumulative extracted water volumes were within 5% of those in corresponding input volumes. Alternating nutrient loads from high to low levels in the fixed flow rate input waters caused excess MRP (1.6 times that in the high concentration MRP solution) to leach from the calcareous soil. The dynamic character of P transport in K-fertilized soils deserves further study and may have important environmental implications.

Automation↗