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Impact seeding and reseeding in the inner solar system.

Assuming that asteroidal and cometary impacts onto Earth can liberate material containing viable microorganisms, we studied the subsequent distribution of the escaping impact ejecta throughout the inner Solar System on time scales of 30,000 years. Our calculations of the delivery rates of this terrestrial material to Mars and Venus, as well as back to Earth, indicate that transport to great heliocentric distances may occur in just a few years and that the departure speed is significant. This material would have been efficiently and quickly dispersed throughout the Solar System. Our study considers the fate of all the ejected mass (not just the slowly moving material), and tabulates impact rates onto Venus and Mars in addition to Earth itself. Expressed as a fraction of the ejected particles, roughly 0.1% and 0.001% of the ejecta particles would have reached Venus and Mars, respectively, in 30,000 years, making the biological seeding of those planets viable if the target planet supported a receptive environment at the time. In terms of possibly safeguarding terrestrial life by allowing its survival in space while our planet cools after a major killing thermal pulse, we show via our 30,000- year integrations that efficient return to Earth continues for this duration. Our calculations indicate that roughly 1% of the launched mass returns to Earth after a major impact regardless of the impactor speed; although a larger mass is ejected following impacts at higher speeds, a smaller fraction of these ejecta is returned. Early bacterial life on Earth could have been safeguarded from any purported impact-induced extinction by temporary refuge in space.

Earth, Planet↗

Probing the domain structure of FtsZ by random truncation and insertion of GFP.

Random transposon-mediated mutagenesis has been used to create truncations and insertions of green fluorescent protein (GFP), and Venus-yellow fluorescent protein (YFP), in Escherichia coli FtsZ. Sixteen unique insertions were obtained, and one of them, in the poorly conserved C-terminal spacer, was functional for cell division with the Venus-YFP insert. The insertion of enhanced GFP (eGFP) at this same site was not functional; Venus-YFP was found to be superior to eGFP in other respects too. Testing the constructs for dominant negative effects led to the following general conclusion. The N-terminal domain, aa 1-195, is an independently folding domain that can poison Z-ring function when expressed without a functional C-terminal domain. The effects were weak, requiring expression of the mutant at 3-5 times the level of wild-type FtsZ. The C-terminal domain, aa 195-383, was also independently folding, but had no activity in vivo. The differential activity of the N- and C-terminal domains suggests that FtsZ protofilament assembly is directional, with subunits adding primarily at the bottom of the protofilament. Directional assembly could occur by either a treadmilling or a dynamic instability mechanism.

Bacterial Proteins↗

Perceptions of beauty in Renaissance art.

The Renaissance was a cultural revolution that spread from Florence, in 1400, throughout Italy and into the rest of Europe. Its impetus was the philosophy of Humanism, which strove to resurrect and emulate the literature and art of the ancient Greeks and Romans. Artists had previously been limited to formulaic religious iconography. They now began to reproduce descriptions of classical paintings and copy the antique statues that were being newly rediscovered. The Renaissance artist's perception of beauty was therefore determined by his philosophical environment, his visual experience (the 'period eye'), the demands of his patrons and by attempts to enhance his professional status in society to equal that of poets and architects. The image of Venus portrayed by Botticelli as the idealization of beauty in Renaissance Florence is significantly different from the Venus portrayed by the German artist, Lucas Cranach. The northern European Venus is much less voluptuous than her Italian counterpart but is still inspired by humanist principals and retains considerable sexuality. Raphael's paintings epitomise the idealization of female beauty of this period but, by his own admission they were rarely based on real models. Often the same facial type was repeated in many different paintings. Indeed Renaissance portrait artists tended to avoid realistic interpretation, emphasizing instead the positive attributes of their subjects, both physical and political. Thus, Bronzino's Portrait of a Young Man not only depicts his subject's idealized appearance but also his scholarship, background and potential. The depiction of beauty in Renaissance art is shown to be more complex than a mere photograph-like representation of sexuality or of a person's physical appearance. Instead, Renaissance art created physically perfect images resulting from scholarly expectation, the artist's ambitions and his developing skills.

Journal Article↗

High-resolution calculations of asteroid impacts into the Venusian atmosphere.

We present results from a number of 2D high-resolution hydrodynamical simulations of asteroids striking the atmosphere of Venus. These cover a wide range of impact parameters (velocity, size, and incidence angle), but the focus is on 2-3 km diameter asteroids, as these are responsible for most of the impact craters on Venus. Asteroids in this size range are disintegrated, ablated, and significantly decelerated by the atmosphere, yet they retain enough impetus to make large craters when they meet the surface. We find that smaller impactors (diameter <1-2 km) are better described by a "pancaking" model in which the impactor is compressed and distorted, while for larger impactors (>2-3 km) fragmentation by mechanical ablation is preferred. The pancaking model has been modified to take into account effects of hydrodynamical instabilities. The general observation that most larger impactors disintegrate by shedding fragments generated from hydrodynamic instabilities spurs us to develop a simple heuristic model of the mechanical ablation of fragments based on the growth rates of Rayleigh-Taylor instabilities. Although in principle the model has many free parameters, most of these have little effect provided that they are chosen reasonably. In practice the range of model behavior can be described with one free parameter. The resulting model reproduces the mass and momentum fluxes rather well, doing so with reasonable values of all physical parameters.

Atmosphere↗

Inorganic chemistry of O2 in a dense primitive atmosphere

A simple steady-state photochemical model is developed in order to determine typical molecular oxygen concentrations for a comprehensive range of primitive abiotic atmospheres. Carbon dioxide is assumed to be the dominant constituent in these atmospheres since CO2 photodissociation may potentially result in the enhancement of the O2 partial pressure. The respective effects of the H2O content, temperature, eddy diffusion coefficient and UV flux on the results are investigated. It is shown that for any pressure at the surface, the partial pressure of molecular oxygen does not exceed 10 mbar. The peculiar case of a runaway greenhouse which has possibly taken place on Venus is qualitatively envisaged. Although O2 is basically absent in the present Venus atmosphere, a transient presence in a primitive stage cannot be ruled out. Possible mechanisms for O2 removal in such an atmosphere are reviewed. At the present stage, we think that the detection of large O2 amounts would be at least a good clue for the presence of life on an extrasolar planet.

Atmosphere↗

A sulfur-based survival strategy for putative phototrophic life in the venusian atmosphere.

Several observations indicate that the cloud deck of the venusian atmosphere may provide a plausible refuge for microbial life. Having originated in a hot proto-ocean or been brought in by meteorites from Earth (or Mars), early life on Venus could have adapted to a dry, acidic atmospheric niche as the warming planet lost its oceans. The greatest obstacle for the survival of any organism in this niche may be high doses of ultraviolet (UV) radiation. Here we make the argument that such an organism may utilize sulfur allotropes present in the venusian atmosphere, particularly S(8), as a UV sunscreen, as an energy-converting pigment, or as a means for converting UV light to lower frequencies that can be used for photosynthesis. Thus, life could exist today in the clouds of Venus.

Atmosphere↗

In vivo imaging and differential localization of lipid-modified GFP-variant fusions in embryonic stem cells and mice.

The visualization of live cell behaviors operating in situ combined with the power of mouse genetics represents a major step toward understanding the mechanisms regulating embryonic development, homeostasis, and disease progression in mammals. The availability of genetically encoded fluorescent protein reporters, combined with improved optical imaging modalities, have led to advances in our ability to examine cells in vivo. We developed a series of lipid-modified fluorescent protein fusions that are targeted to and label the secretory pathway and the plasma membrane, and that are amenable for use in mice. Here we report the generation of two strains of mice, each expressing a spectrally distinct lipid-modified GFP-variant fluorescent protein fusion. The CAG::GFP-GPI strain exhibited widespread expression of a glycosylphosphatidylinositol-tagged green fluorescent protein (GFP) fusion, while the CAG::myr-Venus strain exhibited widespread expression of a myristoyl-Venus yellow fluorescent protein fusion. Imaging of live transgenic embryonic stem (ES) cells, either live or fixed embryos and postnatal tissues demonstrated that glycosylphosphatidyl inositol- and myristoyl-tagged GFP-variant fusion proteins are targeted to and serve as markers of the plasma membrane. Moreover, our data suggest that these two lipid-modified protein fusions are dynamically targeted both to overlapping as well as distinct lipid-enriched compartments within cells. These transgenic strains not only represent high-contrast reporters of cell morphology and plasma membrane dynamics, but also may be used as in vivo sensors of lipid localization. Furthermore, combining these reporters with the study of mouse mutants will be a step forward in understanding the inter- and intracellular behaviors underlying morphogenesis in both normal and mutant contexts.

Animals↗

The 'Pseudorhabdosynochus cupatus group' (Monogenea: Diplectanidae) on Epinephelus fasciatus, E. howlandi, E. rivulatus and E. merra (Perciformes: Serranidae) off New Caledonia, with descriptions of Pseudorhabdosynochus cyathus n. sp. and P. calathus n. sp.

Pseudorhabdosynochus cupatus (Young, 1969) is characterised by small lamellosquamodiscs made up of central telescopic rings and peripheral rows of rodlets, and a sclerotised vagina in the shape of a thin-walled coiled tube with three accessory chambers. Two new species of Pseudorhabdosynochus are herein described from epinepheline fish off New Caledonia, South Pacific, and are considered part of the 'P. cupatus group'. P. cyathus n. sp. from Epinephelus howlandi has lamellosquamodiscs made up of four telescopic rings and four rows of separate rodlets; there are dorsal tegumental scales. This species was found only in young hosts; older hosts harbour P. venus Hinsinger & Justine, 2006. P. calathus n. sp. from Epinephelus rivulatus has lamellosquamodiscs made up of four telescopic rings and five rows of separate rodlets; the tegument is smooth. The three species, P. cupatus, P. calathus and P. cyathus, are strictly species specific; although morphologically very similar, they can be distinguished by features of the tegumental scales and measurements of the vagina and haptoral hard parts. P. melanesiensis (Laird, 1958) from E. merra is close to the 'P. cupatus group' according to the morphology of its sclerotised vagina but is distinguished by its squamodiscs which lack telescopic rings. Rare specimens found in E. merra are tentatively attributed to P. coioidesis Bu et al., 1999. A paratype of P. coioidesis is figured for comparison. E. merra, E. fasciatus, E. rivulatus and E. howlandi each have a Pseudorhabdosynochus species of the P. cupatus lineage: these are the dominant species in each host. In addition, E. merra, E. fasciatus and E. howlandi harbour a rare species of Pseudorhabdosynochus, respectively P. cf. coioidesis, P. caledonicus Justine, 2005 and P. venus; these rare species are morphologically unrelated to each other and to the 'P. cupatus group'.

Animals↗

Relationship between copper speciation in sediments and bioaccumulation by marine bivalves of Taiwan.

This paper evaluates the relationships between copper species in sediments and accumulation by the purple clam (Hiatula diphos) and venus clam (Gomphina aeguilatera) collected from the field and culture (aquaculture) ponds in the polluted coastal area of Lukang, Taiwan. Sediment was sampled along with the molluscs, including oysters (Crassostrea gigas), purple clams (Hiatula diphos), rock-shells (Thais clavigera), venus clams (Gomphina aeguilatera), and hard clams (Meretrix lusoria), from two unique environments of Lukang during the period from August 1993 to July 1994. The data indicate that the total copper concentrations in sediments from culture ponds (185 microg g(-1)) was higher than those of the field (44.0 microg g(-1)). Copper species in sediments were analyzed by a sequential leaching technique. Results show that concentrations of various copper species in the sediments are in the range of 1.14 +/- 0.59 to 13.2 +/- 22.4 microg g(-1) and 0.36 +/- 0.24 to 133 +/- 36.7 microg g(-1) for the two environments, respectively. Also the exchangeable copper in sediment from culture ponds was 15 times higher than that from the field. In addition, the sum of exchangeable and copper carbonates had the highest percentages of copper in both the pond sediment (86.6 %) and the field sediment (50.7 %). Maximum copper concentrations (309 +/- 35.1 microg g(-1)) in oysters were much higher than those in the other benthic organisms by about 4-127 times. Similarly, the data also showed that copper concentrations in Thais clavigera were 12-32 times higher than those in other benthic organisms. Copper concentrations in various benthic organisms differed significantly (p < 0.05) from that in Thais clavigera. This capacity makes Thais clavigera a potential candidate for monitoring copper in marine sediments. In terms of copper species, the best correlation was generally obtained between copper carbonates in sediments and copper concentrations in Hiatula diphos (r = 0.886*). A strong multiple regression correlation (p < 0.05, r2 = 0.7894) also indicates that the copper carbonates may dominate as the available form of copper to Hiatula diphos from various environments in the Lukang coastal area under natural physicochemical conditions.

Journal Article↗

Records of polychlorinated biphenyls (PCBs) in sediments of four remote Chilean Andean Lakes.

Sediment cores from four Chilean lakes along the Andes Chain (Chungará, Laja, Castor and Venus) were analysed in order to investigate PCB concentrations and distributions in sediment samples. Sediment cores were analysed for PCBs using gas chromatography (GC-ECD/MS) and radioisotopically dated using 210Pb. Organic carbon content (OC) and 210Pb fluxes were also measured. Results showed that sediment PCB concentrations (ngg(-1) d.w.) at Lake Chungará (1.2 +/- 1) in northern Chile, Laja (5 +/- 4) in central, and in Lake Castor (3.5 +/- 4) in southern Chile (the eastern side of the Andes Mountain) were lower than sediments collected from Lake Venus (64 +/- 30) located in southern Chile (the western side) which contained 15-fold higher concentrations of PCBs. The percentage (%) of organic carbon was variable and showed a high range of values in the sediment fluctuating from 2% (Lake Laja) to 22% (Lake Chungará). Analysis of 210Pb fluxes, presented a decrease trend following Lake Laja>Castor>Chungará with a positive correlation with rainfall at each site. Sedimentation rates in Lake Castor (1846 gm(-2)yr(-1)) were higher than at Chungará (748 gm(-2)yr(-1)) and Lake Laja (508 gm(-2)yr(-1)). Focusing factor (FF) is used as a tool to elucidate PCB input in the aquatic ecosystem. FF were lower (<1) for the shallower lakes (Lakes Chungará and Castor). This study provides background levels of PCBs at remote lakes in Chile. Differences in geographical characteristics (orographic effect) might play an important role in the arrival of PCBs, particularly into the southern lakes. PCB fluxes indicated deposition of PCBs in recent sediments is higher than in previous years with peaks of PCB between 1991 and 1998. The continuing increase of PCB inputs in remote Chilean lakes, could be associated with long range atmospheric transport (LRAT).

Carbon↗

Distribution of organotin compounds in the bivalves of the Aegean Sea, Greece.

Five bivalve species--Mytilus galloprovinciallis (Mediterranean mussels), Venus gallina (stripped venus), Modiola barbatus L. (bearded horse mussels), Pecten jacobeus (scallops) and Callista chione (hard clams)--were collected from seven areas in Aegean Sea, Greece, between August 2001 and January 2003 and analyzed for organotins (OTs). The concentrations (as geometric means) found were 17.1 ng g-1 for tributyltin (TBT), 18.8 ng g-1 for dibutytltin (DBT), 7.8 ng g-1 for monobutyltin (MBT) and 13.0 ng g-1 for triphenyltin (TPhT) (wet weight), which are at similar or lower levels than those reported worldwide. Studying OTs distribution between different bivalve species, lower concentrations were observed in mediterranean mussels, possibly due to their growth in water column (grown on sea net pens in mussel farms), in contrast to the free-ranging species, collected from fishing grounds. Concentrations of the OTs in the examined bivalves varied seasonally.

Animals↗

HVJ-envelope vector for gene transfer into central nervous system.

To overcome some problems of virus vectors, we developed a novel non-viral vector system, the HVJ-envelope vector (HVJ-E). In this study, we investigated the feasibility of gene transfer into the CNS using the HVJ-E both in vitro and in vivo. Using the Venus reporter gene, fluorescence could be detected in cultured rat cerebral cortex neurons and glial cells. In vivo, the reporter gene (Venus) was successfully transfected into the rat brain by direct injection into the thalamus, intraventricular injection, or intrathecal injection, without inducing immunological change. When the vector was injected after transient occlusion of the middle cerebral artery, fluorescence due to EGFP gene or luciferase activity could be detected only in the injured hemisphere. Finally, luciferase activity was markedly enhanced by the addition of 50 U/ml heparin (P<0.01). Development of efficient HVJ-E for gene transfer into the CNS will be useful for research and clinical gene therapy.

Animals↗

Study of paralytic shellfish poisoning toxin profile in shellfish from the Mediterranean shore of Morocco.

Since 1992, a monitoring program for bivalve molluscs contaminated by algal toxins was established at different stations along the Mediterranean Moroccan shores. The monitored stations were tested every 2 weeks. The presence of toxicity was determined using the mouse bioassay method. Toxin profile was carried out by HPLC/FD in selected contaminated tissues. According to the outcomes of this surveillance from 1994 to 1999, reliable information on toxicity of shellfish was obtained. They indicate that PSP is a recurrent toxicity in molluscs along the Mediterranean shore of Morocco. It has been noted a difference of PSP accumulation among individual shellfish. The cockle (Achanthocardia tuberculatum) presents toxicity throughout the year, while other specimens from the same area such as clam (Callista chione), warty venus (Venus gallina) and marine beans (Donax trunculus) accumulate it seasonally from January to April, after which they depurate the toxin. Moreover, the study of toxin profiles among individual shellfish was undertaken. It was found that shellfish presented a complex profile pointing to contamination by Gymnodinium catenatum.

Animals↗

Naturally occurring mutations of the extracellular Ca2+-sensing receptor: implications for its structure and function.

The extracellular Ca2+-sensing receptor is a member of the G-protein-coupled receptor family 3 in which agonists bind to a dimeric Venus-flytrap domain in the extracellular portion of the receptor. How agonist binding to this domain leads to activation of the seven-transmembrane domain is a major unresolved question. Information derived from the three-dimensional structure of the Venus-flytrap domain of the related metabotropic glutamate type 1 receptor, and from naturally occurring mutations of the Ca2+-sensing receptor identified in subjects with familial hypocalciuric hypercalcemia and autosomal dominant hypocalcemia offers new insights into the mechanism of receptor activation, and into the mechanism of action of allosteric modulators of the receptor.

Amino Acid Sequence↗

Opening and closing motions in the periplasmic vitamin B12 binding protein BtuF.

BtuF is the periplasmic binding protein (PBP) in the vitamin B(12) uptake system in Escherichia coli where it is associated with the ABC transporter BtuCD. When the ligand binds, PBPs generally display large conformational changes, commonly termed the Venus flytrap mechanism. BtuF belongs to a group of PBPs that, on the basis of crystal structures, does not appear to display such behavior. Using 480 ns multicopy molecular dynamics simulations of apo and holo forms of the protein, we investigate the dynamics of BtuF. We find BtuF to be more flexible than previously assumed, displaying clear opening and closing motions which are more pronounced in the apo form. The protein behavior is compatible with a PBP functional model that postulates a closed conformation for the ligand-bound state, whereas the empty form fluctuates between open and closed conformations. Elastic network normal-mode analysis suggests that all BtuF-like PBPs are capable of similar opening and closing motions. It also makes the typical Venus flytrap domain motions a likely common means of how PBP-ABC transporter interaction could occur.

Escherichia coli↗

Optical efficiencies of lightning in planetary atmospheres.

Spacecraft observations show that the presence of lightning activity is not confined to the terrestrial atmosphere, but is also found in the atmospheres of Venus, Jupiter and Saturn. Lightning activity may also occur in Titan's thick atmosphere. Calculations show that lightning produces a significant fraction of the nitric oxide that reacts with the ozone and chlorine compounds in the terrestrial stratosphere. In the atmosphere of the primordial Earth, lightning could have been the major source of many of the molecules required for the formation of life. To determine the effects of lightning activity in the atmospheres of other planets from spacecraft images requires a knowledge of the optical properties of the lightning discharge. Here we report the first simulations of lightning in planetary atmospheres by laser-induced plasmas. These simulations show that the fraction of the energy in lightning discharge channels that is radiated in the visible spectrum is similar for Earth, Venus and Titan, but quite different for Jupiter. One implication of our results is that the amount of trace gases produced by lightning in the jovian atmosphere must be larger than previously estimated.

Astronomy↗

Discovery of an aurora on Mars.

In the high-latitude regions of Earth, aurorae are the often-spectacular visual manifestation of the interaction between electrically charged particles (electrons, protons or ions) with the neutral upper atmosphere, as they precipitate along magnetic field lines. More generally, auroral emissions in planetary atmospheres "are those that result from the impact of particles other than photoelectrons" (ref. 1). Auroral activity has been found on all four giant planets possessing a magnetic field (Jupiter, Saturn, Uranus and Neptune), as well as on Venus, which has no magnetic field. On the nightside of Venus, atomic O emissions at 130.4 nm and 135.6 nm appear in bright patches of varying sizes and intensities, which are believed to be produced by electrons with energy <300 eV (ref. 7). Here we report the discovery of an aurora in the martian atmosphere, using the ultraviolet spectrometer SPICAM on board Mars Express. It corresponds to a distinct type of aurora not seen before in the Solar System: it is unlike aurorae at Earth and the giant planets, which lie at the foot of the intrinsic magnetic field lines near the magnetic poles, and unlike venusian auroras, which are diffuse, sometimes spreading over the entire disk. Instead, the martian aurora is a highly concentrated and localized emission controlled by magnetic field anomalies in the martian crust.

Journal Article↗

A transgenic mouse model for monitoring endoplasmic reticulum stress.

Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. Although the connection between ER stress and some disease-related proteins has been studied using animal models of these diseases, no in vivo data concerning ER stress are available. Here we report a new method for monitoring ER stress in vivo, based on XBP-1 mRNA splicing by inositol requiring-1 (IRE-1) during ER stress. The stress indicator was constructed by fusing XBP-1 and venus, a variant of green fluorescent protein. During stress, the spliced indicator mRNA is translated into an XBP-1-venus fusion protein, which can be detected by its fluorescence. We used transgenic animals expressing the ER stress indicator to show that it can be used to monitor physiological and pathological ER stress in vivo.

Animals↗