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At least 235 records · Page 13Linked to original sources
PubMed1993

A new method for the detection of viable cells in tissue sections using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT): an application in the assessment of tissue damage by surgical instruments.

This report describes a new method using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to visualize live viable tissue at the microscopic level. The visualization of the MTT stained tissue provides a metabolic assessment of tissue damage, and can be utilized as an extension of conventional hematoxylin-eosin (H & E) staining. In this report, several tissues were studied with MTT and H & E staining after incisions had been made by a variety of surgical dissecting instruments. A marked improvement in the detection of tissue damage was seen using MTT, regardless of how the damage was caused, i.e., physical, heat, or photon energy. In addition, a distinct zone of damage not noted on conventionally prepared and stained tissues is readily apparent. Thus MTT staining will have utility in both clinical and research studies, concerned with assessing the viability of tissues.

Animals↗
PubMed1997

[From the history of surgical instruments: 6. Sauerbruch's instruments for thoracic surgery].

The historical development of thoracic surgery, which was established in Germany in the years 1904/1905 and the armamentarium of the chest designed by Ferdinand Sauerbruch (1875-1951) are described. Sauerbruch designed between 1904 and 1920 many instruments to facilitate thoracic surgery, e.g. several rib retractors, a special rib shear for the first rib, a rib shear for all ribs other than the first. Furthermore he designed a special lung grasping forceps with rubber covered jaws, a lung compression forceps and a fenestrated lung retractor to facilitate lung resections.

Germany↗
PubMed1995

[History of surgical instruments: 2. The first atraumatic vascular clamp by E. Höpfner (1903)].

The author describes one of the first atraumatic vascular clamps specifically developed for vascular surgery in 1903, and used for temporary arterial occlusion. This forceps was about 15 cm long, and its spring steel jaws were separated in the middle, but approximated toward the ends. This clamp was developed by Edmund Höpfner (b. 1873) in 1903, at the Berlin University Hospital, department of Surgery, when he performed end-to-end anastomoses of divided arteries in dogs as part of his doctoral thesis. The Höpfner clamp was in common use in Germany until the 1960s.

Anastomosis, Surgical↗