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Human locomotion on snow: determinants of economy and speed of skiing across the ages.

We explore here the evolution of skiing locomotion in the last few thousand years by investigating how humans adapted to move effectively in lands where a cover of snow, for several months every year, prevented them from travelling as on dry ground. Following historical research, we identified the sets of skis corresponding to the 'milestones' of skiing evolution in terms of ingenuity and technology, built replicas of them and measured the metabolic energy associated to their use in a climate-controlled ski tunnel. Six sets of skis were tested, covering a span from 542 AD to date. Our results show that: (i) the history of skiing is associated with a progressive decrease in the metabolic cost of transport, (ii) it is possible today to travel at twice the speed of ancient times using the same amount of metabolic power and (iii) the cost of transport is speed-independent for each ski model, as during running. By combining this finding with the relationship between time of exhaustion and the sustainable fraction of metabolic power, a prediction of the maximum skiing speed according to the distance travelled is provided for all past epochs, including two legendary historical journeys (1206 and 1520 AD) on snow. Our research shows that the performances in races originating from them (Birkebeiner and Vasaloppet) and those of other modern competitions (skating versus classical techniques) are well predicted by the evolution of skiing economy. Mechanical determinants of the measured progression in economy are also discussed in the paper.

Adult↗

Mechanical properties of granular media, including snow, investigated by a low-frequency forced torsion pendulum

The oscillating probe of a low-frequency forced torsion pendulum is immersed into various granular media, such as natural sand, glass beads, and granular snow. A first layer of particles is in general solidly bound to the probe surface. The principle of operation and a rheological model are presented. The measured dynamic moduli systematically show a peak of the loss factor and a step in the absolute modulus. The effect of moisture-induced aging in glass beads of small size and the effect of sintering of ice grains in snow are investigated. The response of the pendulum is determined by the long-range statistical properties of force chains opposing the rotation of the pendulum, and by the tribological processes that take place at the grain contacts.

Journal Article↗

Snow-mold-induced apoplastic proteins in winter rye leaves lack antifreeze activity

During cold acclimation, winter rye (Secale cereale L.) plants secrete antifreeze proteins that are similar to pathogenesis-related (PR) proteins. In this experiment, the secretion of PR proteins was induced at warm temperatures by infection with pink snow mold (Microdochium nivale), a pathogen of overwintering cereals. A comparison of cold-induced and pathogen-induced proteins showed that PR proteins accumulated in the leaf apoplast to a greater level in response to cold. The PR proteins induced by cold and by snow mold were similar when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and examined by immunoblotting. Both groups of PR proteins contained glucanase-like, chitinase-like, and thaumatin-like proteins, and both groups exhibited similar levels of glucanase and chitinase activities. However, only the PR proteins induced by cold exhibited antifreeze activity. Our findings suggest that the cold-induced PR proteins may be isoforms that function as antifreeze proteins to modify the growth of ice during freezing while also providing resistance to the growth of low-temperature pathogens in advance of infection. Both functions of the cold-induced PR proteins may improve the survival of overwintering cereals.

Journal Article↗

Morphological possibilities in general crystallography. Snow crystals.

Morphological features of snow crystals are analyzed on the basis of concepts of a general crystallography, where point groups of infinite order are possible. The observations are first formulated in a set of rules, leading to a macroscopic growth lattice and to continuous growth boundaries. Both are brought in connection with two-dimensional integral invertible transformations. Families of boundaries are considered, labeled by a set of indices restricted by selection rules and generalizing the law of rational indices. These properties are indicated graphically on a sample of 12 natural snow crystals. Their geometric and arithmetic properties are summarized in a table.

Journal Article↗

The genetic legacy of Mother Goose--phylogeographic patterns of lesser snow goose Chen caerulescens caerulescens maternal lineages.

By using the polymerase chain reaction to amplify and sequence 178 bp of a rapidly evolving region of the mtDNA genome (segment I of the control region) from 81 individuals, approximately 11% of the variation present in the lesser snow goose Chen caerulescens caerulescens L. mitochondrial genome was surveyed. The 26 types of mtDNA detected formed two distinct mitochondrial clades that differ by an average of 6.7% and are distributed across the species range. Restriction analysis of amplified fragments was then used to assign the mtDNA of an additional 29 individuals to either of these clades. Within one major clade, sequence among mtDNAs was concordant with geographic location. Within the other major clade the degree of sequence divergence among haplotypes was lower and no consistent geographic structuring was evident. The two major clades presumably result from vicariant separation of lesser snow geese during the Pleistocene.

Animals↗

Deuterium content of snow cores from Sierra Nevada area.

The relative deuterium content was measured on 37 snow cores collected in April 1969 in the Sierra Nevada. The deuterium content varies inversely with altitude of collection (approximately 40 per mil per 1000 meters) but is unrelated to latitude. The altitude relationship is particularly well defined west of the crest of the range but is not well defined east of the crest. However, samples from east of the crest tend to be depleted by about 10 to 15 per mil relative to samples collected at the same elevation west of the crest. We propose that the deuterium content of snow cores, collected so as to include the total winter's precipitation, can be used as a climatic indicator to compare the climate of one winter with that of another.

Altitude↗

Highly active microbial communities in the ice and snow cover of high mountain lakes.

An exploratory study carried out in Pyrenean and Alpine lakes shows that a rich, active microbial community lives in the slush layers of the winter cover of such lakes in spite of the low temperature and the seasonal occurrence of the habitat. Bacteria were very diverse in morphology, with filaments reaching up to 100 (mu)m long; flagellates, both autotrophic (chrysophytes, cryptophytes, dinoflagellates, and volvocales) and heterotrophic, and ciliates were abundant, reaching biovolume values up to 2.7 x 10(sup6) (mu)m(sup3) ml(sup-1). Species composition was very variable, with dominance depending on date and depth. Although many species were typical of lake plankton communities, some were restricted to the slush, for instance the predatory ciliates Dileptus sp. and Lacrymaria sp., and others were restricted to the surface pools, such as the snow algae Chlamydomonas nivalis. Microbial biomasses and usually bacterial and algal activities were greater in the slush layers than in the lake water. Photosynthesis rate in the upper cover layers reached values up to 0.5 (mu)g of C liter(sup-1) h(sup-1), and high bacterial activities up to 226 pmol of leucine incorporated liter(sup-1) h(sup-1) and 25 pmol of thymidine incorporated liter(sup-1) h(sup-1) were measured. For most species, lake water flooding the ice and snow cover could provide an inoculum. Differential growth depending on the environmental conditions (nutrients, organic matter, light) of a particular slush layer could provide dominance of different groups or species. However, there was no obvious colonizing mechanism for those species not appearing either in plankton or in communities on top of the snowpack.

Journal Article↗

Alpha- and beta-Proteobacteria control the consumption and release of amino acids on lake snow aggregates.

We analyzed the composition of aggregate (lake snow)-associated bacterial communities in Lake Constance from 1994 until 1996 between a depth of 25 m and the sediment surface at 110 m by fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes of various specificity. In addition, we experimentally examined the turnover of dissolved amino acids and carbohydrates together with the microbial colonization of aggregates formed in rolling tanks in the lab. Generally, between 40 and more than 80% of the microbes enumerated by DAPI staining (4',6'-diamidino-2-phenylindole) were detected as Bacteria by the probe EUB338. At a depth of 25 m, 10.5% +/- 7.9% and 14.2% +/- 10.2% of the DAPI cell counts were detected by probes specific for alpha- and beta-Proteobacteria. These proportions increased to 12.0% +/- 3.3% and 54.0% +/- 5.9% at a depth of 50 m but decreased again at the sediment surface at 110 m to 2.7% +/- 1.4% and 41.1% +/- 8.4%, indicating a clear dominance of beta-Proteobacteria at depths of 50 and 110 m, where aggregates have an age of 3 to 5 and 8 to 11 days, respectively. From 50 m to the sediment surface, cells detected by a Cytophaga/Flavobacteria-specific probe (CF319a) comprised increasing proportions up to 18% of the DAPI cell counts. gamma-Proteobacteria always comprised minor proportions of the aggregate-associated bacterial community. Using only two probes highly specific for clusters of bacteria closely related to Sphingomonas species and Brevundimonas diminuta, we identified between 16 and 60% of the alpha-Proteobacteria. In addition, with three probes highly specific for close relatives of the beta-Proteobacteria Duganella zoogloeoides (formerly Zoogloea ramigera), Acidovorax facilis, and Hydrogenophaga palleroni, bacteria common in activated sludge, 42 to 70% of the beta-Proteobacteria were identified. In the early phase (<20 h) of 11 of the 15 experimental incubations of aggregates, dissolved amino acids were consumed by the aggregate-associated bacteria from the surrounding water. This stage was followed by a period of 1 to 3 days during which dissolved amino acids were released into the surrounding water, paralleled by an increasing dominance of beta-Proteobacteria. Hence, our results show that lake snow aggregates are inhabited by a community dominated by a limited number of alpha- and beta-Proteobacteria, which undergo a distinct succession. They successively decompose the amino acids bound in the aggregates and release substantial amounts into the surrounding water during aging and sinking.

Alphaproteobacteria↗

Enzyme-linked immunosorbent assays for Snow Mountain and Norwalk agents of viral gastroenteritis.

Enzyme-linked immunosorbent assays (ELISAs) for antigen detection and blocking ELISAs for serum antibody rises were developed for the Snow Mountain and Norwalk agents of viral gastroenteritis. The ELISAs were as sensitive as the existing radioimmunoassays and were specific for the Snow Mountain or Norwalk agent. The blocking ELISAs detected the same number of significant rises in antibodies to these agents as did the existing blocking radioimmunoassays.

Antibodies, Viral↗

Expression, self-assembly, and antigenicity of a snow mountain agent-like calicivirus capsid protein.

Virus-like particles were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of MX virus, a Mexican strain of human calicivirus. These recombinant MX (rMX) particles were morphologically similar to recombinant Norwalk virus (rNV) particles as observed under an electron microscope and contained a single capsid protein with a molecular weight of 57,000, which was slightly smaller than that of rNV. This protein was immunoprecipitated by sera from volunteers infected with the Snow Mountain agent, but it reacted weakly with sera from volunteers infected with NV. This protein did not react with hyperimmune antisera from animals immunized with rNV in the rNV antigen enzyme immunoassay (EIA). Seroresponses were detected from volunteers infected with Snow Mountain agent and Hawaii agent when the rMX particles were used as antigen in an EIA. This EIA also detected an immune response in the sera of child from whom the MX virus was isolated, and a high prevalence of antibody to MX virus was found in the sera of a cohort of Mexican children.

Animals↗

Snow sports injuries in Scotland: a case-control study.

OBJECTIVES: To examine the incidence and patterns of snow sports injuries at the three largest commercial ski areas in Scotland and to identify factors associated with injury risk. METHODS: A prospective case-control study of all injured people at Cairngorm, Glenshee, and Nevis Range ski areas during the 1999-2000 winter season. Personal details, snow sports related variables, diagnosis, and treatment were recorded. Control data were collected at random from uninjured people at all three areas. Random counts were performed to analyse the composition of the on slope population. RESULTS: A total of 732 injuries were recorded in 674 people. Control data were collected from 336 people. The injury rate for the study was 3.7 injuries per 1000 skier days. Alpine skiers comprised 67% of the on slope population, snowboarders 26%, skiboarders 4%, and telemark skiers 2%. Lower limb injuries and sprains were the commonest injuries in alpine skiers and skiboarders. Snowboarders sustained more injuries to the upper limb and axial areas. Skiboarders and snowboarders had a higher incidence of fractures. After adjustment for other variables, three factors were all independently associated with injury: snowboarding (odds ratio (OR) 4.07, 95% confidence interval (CI) 1.65 to 10.08), alpine skiing (OR 3.82, CI 1.6 to 9.13), and age <16 years (OR 1.9, CI 1.14 to 3.17). More than five days of experience in the current season and at least one week of experience in total had a protective effect against injury. CONCLUSIONS: Despite a change in the composition of the alpine population at Scottish ski areas, the overall rate and pattern of injury are similar to those reported previously in comparable studies. Several factors are associated with an increased risk of injury and should be targeted in future injury prevention campaigns.

Adolescent↗

Patterns of improvement in spirometry, bronchial hyperresponsiveness, and specific IgE antibody levels after cessation of exposure in occupational asthma caused by snow-crab processing.

Thirty-one workers with occupational asthma caused by snow-crab processing were assessed by a long-term follow-up on three occasions at mean +/- SD intervals of 12.8 +/- 5.4, 31.4 +/- 6.3, and 64.4 +/- 6.3 months after leaving work. The diagnosis of work-related asthma was initially confirmed in all of them by specific inhalation challenges at the workplace or by laboratory inhalation of snow-crab boiling water (n = 24) or by serial monitoring of airway caliber and bronchial responsiveness to histamine at work and off work (n = 7). Total duration of work-related exposure was 12.8 +/- 5.6 months (range, 3 to 21 months), and the duration of symptoms after onset was 6.8 +/- 4.2 months (range, 1 to 18 months). At the time of diagnosis, all 31 subjects required medication for asthma, 11 had a FEV1 less than or equal to 85% predicted, and all subjects had a PC20 less than or equal to 16 mg/ml. Twelve of 25 serum samples assessed showed high levels of specific immunoglobulin E (IgE) antibodies to crab meat and/or boiling water. At the time of the first follow-up, there was a reduction in the number of subjects still requiring medication, with a significant reduction in FEV1, and a PC20 less than or equal to 16 mg/ml. However, no further change was observed afterwards. Similarly, the mean FEV1 and FEV1/FVC improved significantly from the time of diagnosis to the first follow-up (p less than 0.01), with a plateau thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

Veno-occlusive disease in snow leopards (Panthera uncia) from zoological parks.

Livers from 54 snow leopards, 4 days to 23 years old, that had died in 23 US zoos, were evaluated histopathologically to determine if the hepatic fibrosis, which has been noted to be prevalent in this species, was due to chronic active hepatitis from hepadnaviral infection, Ito cell proliferation, or hemosiderosis. Forty-two of 54 snow leopards had subintimal vascular fibrosis with partial or total occlusion of central and sublobular veins (veno-occlusive disease) of unknown origin. All 21 leopards older than 5 years were affected. Four leopards had chronic active hepatitis, and 12 leopards had cholangiohepatitis; but these lesions were not connected anatomically to central and sublobular venous fibrosis. Hepatocellular and Kupffer cell siderosis and Ito cell proliferation were prevalent and often coexisted with perisinusoidal, central, and sublobular venous fibrosis; but fibrosis was present in leopards without siderosis or Ito cell proliferation. The pattern and prevalence of veno-occlusive disease in these leopards was similar to that reported in captive cheetah (Acinonyx jubatus), suggesting that a common extrinsic factor may cause the majority of hepatic disease in these large felid animals in captivity.

Animals↗

Orientation of snow buntings (Plectrophenax nivalis) close to the magnetic north pole

Orientation experiments were performed with first-year snow buntings (Plectrophenax nivalis) during their autumn migration in a natural near-vertical geomagnetic field approximately 400 km away from the magnetic north pole. Migratory orientation of snow buntings was recorded using two different techniques: orientation cage tests and free-flight release experiments. Experiments were performed under clear skies, as well as under natural and simulated complete overcast. Several experimental manipulations were performed including an artificial shift of the E-vector direction of polarized light, depolarization of incoming light and a 4 h slow clock-shift experiment. The amount of stored fat proved to be decisive for the directional selections of the buntings. Fat individuals generally chose southerly mean directions, whereas lean birds selected northerly headings. These directional selections seemed to be independent of experimental manipulations of the buntings' access to visual cues even in the local near-vertical magnetic field. Under clear skies, the buntings failed to respond to either a deflection of the E-vector direction of polarized light or an experimental depolarization of incoming skylight. When tested under natural as well as simulated overcast, the buntings were still able to select a meaningful mean direction according to their fat status. Similarly, the free-flight release test under complete overcast resulted in a well-defined southsoutheast direction, possibly influenced by the prevailing light northwest wind. Clock-shift experiments did not yield a conclusive result, but the failure of these birds to take off during the subsequent free-flight release test may indicate some unspecified confusion effect of the treatment.

Journal Article↗

Dirofilaria immitis infection of a snow leopard (Uncia uncia) in a Japanese zoo with mitochondrial DNA analysis.

Three dog heartworms (Dirofilaria immitis) were detected in the lumen of the right cardiac ventriculus and of the pulmonary artery of a captive female snow leopard (Uncia uncia) that died of pancreatic carcinoma at a zoo in Japan. Neither clinical respiratory nor circulatory symptoms caused by the heartworm infection were observed. The filarial worms were identified as D. immitis from the morphologic characteristics of the esophagus, the presence of faint longitudinal ridges on the cuticular surface, the situation of vulva posterior to the esophagus, and the measurements of the body. The heartworms from the snow leopard were identical to that of D. immitis from dogs in the sequence of the cytochrome oxidase I region in the mitochondrial DNA. This host record is the first of D. immitis in U. uncia.

Animals↗

Retrieval of the ultraviolet effective snow albedo during 1998 winter campaign in the French Alps.

A measurement campaign was carried out in February 1998 at Briançon Station, French Alps (44.9 degrees N, 6.65 degrees E, 1,310 m above sea level) in order to determine the UV effective snow albedo that was retrieved for both erythemal and UV-A irradiances from measurements and modeling enhancement factors. The results are presented for 15 cloudless days with very variable snow cover and a small snowfall in the middle of the campaign. Erythemal irradiance enhancement due to the surface albedo was found to decrease from approximately +15% to +5% with a jump to +22% after the snowfall, whereas UV-A irradiance enhancement decreased from 7% to 5% and increased to 15% after the snowfall. Thesevalues fit to effective surface albedos of 0.4, 0.1, and 0.5 for erythemal, and to effective albedos of 0.25, 0.1, and 0.4 for UV-A irradiances, respectively. An unexpected difference between the effective albedos retrieved in the two wavelength regions can be explained by the difference of the environment contribution.

Journal Article↗

Structural modeling of snow flea antifreeze protein.

The glycine-rich antifreeze protein recently discovered in snow fleas exhibits strong freezing point depression activity without significantly changing the melting point of its solution (thermal hysteresis). BLAST searches did not detect any protein with significant similarity in current databases. Based on its circular dichroism spectrum, discontinuities in its tripeptide repeat pattern, and intramolecular disulfide bonding, a detailed theoretical model is proposed for the 6.5-kDa isoform. In the model, the 81-residue protein is organized into a bundle of six short polyproline type II helices connected (with one exception) by proline-containing turns. This structure forms two sheets of three parallel helices, oriented antiparallel to each other. The central helices are particularly rich in glycines that facilitate backbone carbonyl-amide hydrogen bonding to four neighboring helices. The modeled structure has similarities to polyglycine II proposed by Crick and Rich in 1955 and is a close match to the polyproline type II antiparallel sheet structure determined by Traub in 1969 for (Pro-Gly-Gly)(n). Whereas the latter two structures are formed by intermolecular interactions, the snow flea antifreeze is stabilized by intramolecular interactions between the helices facilitated by the regularly spaced turns and disulfide bonds. Like several other antifreeze proteins, this modeled protein is amphipathic with a putative hydrophobic ice-binding face.

Amino Acid Sequence↗