[New solvents and solvent combinations for chromatography of dinitrophenylaminoacids on fine texture paper].
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Thin-layer chromatography and high-performance liquid chromatography (HPLC) with linear photodiode array detection (LPDA) were used to separate impurities from two commercial dyes. Gravity flow liquid chromatography was used to purify reference standards of the dyes. Normal phase HPLC with LPDA detection was used to determine purities of submitted samples by comparing responses to those of the reference standards. Electron impact mass spectrometry and nuclear magnetic resonance were utilized to confirm structures of the dyes and their impurities.
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The phase diagram of the system ethyl cellulose-chloroform-ethane diol has been studied and the differing phase regions determined. The effect of temperature of these regions is recorded. The phase diagram is used to determine the optimal conditions for the preparation of microcapsules with a phenobarbitone core and the size distribution of the microcapsules is recorded.
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