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Habitat assessment of non-wadeable rivers in Michigan.

Habitat evaluation of wadeable streams based on accepted protocols provides a rapid and widely used adjunct to biological assessment. However, little effort has been devoted to habitat evaluation in non-wadeable rivers, where it is likely that protocols will differ and field logistics will be more challenging. We developed and tested a non-wadeable habitat index (NWHI) for rivers of Michigan, where non-wadeable rivers were defined as those of order >or=5, drainage area >or=1600 km2, mainstem lengths >or=100 km, and mean annual discharge >or=15 m3/s. This identified 22 candidate rivers that ranged in length from 103 to 825 km and in drainage area from 1620 to 16,860 km2. We measured 171 individual habitat variables over 2-km reaches at 35 locations on 14 rivers during 2000-2002, where mean wetted width was found to range from 32 to 185 m and mean thalweg depth from 0.8 to 8.3 m. We used correlation and principal components analysis to reduce the number of variables, and examined the spatial pattern of retained variables to exclude any that appeared to reflect spatial location rather than reach condition, resulting in 12 variables to be considered in the habitat index. The proposed NWHI included seven variables: riparian width, large woody debris, aquatic vegetation, bottom deposition, bank stability, thalweg substrate, and off-channel habitat. These variables were included because of their statistical association with independently derived measures of human disturbance in the riparian zone and the catchment, and because they are considered important in other habitat protocols or to the ecology of large rivers. Five variables were excluded because they were primarily related to river size rather than anthropogenic disturbance. This index correlated strongly with indices of disturbance based on the riparian (adjusted R2 = 0.62) and the catchment (adjusted R2 = 0.50), and distinguished the 35 river reaches into the categories of poor (2), fair (19), good (13), and excellent (1). Habitat variables retained in the NWHI differ from several used in wadeable streams, and place greater emphasis on known characteristic features of larger rivers.

Conservation of Natural Resources↗

Radioactive contamination in the upper part of the Techa river: stirring-up of bottom sediments and precipitation of suspended particles. Analysis of the data obtained in 1949-1951.

A hydrodynamic model of the upper part of the Techa river was developed on the basis of the river valley geometry as well as data of hydrological conditions and of the granulometric composition of bottom sediments. The model describes the transport of radioactivity by suspended sediments with different granulometric compositions (clay, silt) in the early 1950s. It includes the stirring-up of bottom sediments and the precipitation of suspended sediments as a function of water discharge rate and water level in the investigated part of the river. The results allow to specify the development of the river system contamination as a result of inflow of suspended sediments contaminated with radionuclides. In the period of liquid radioactive waste (LRW) discharges, the water of the Techa river contained a large fraction of finely dispersed particles of less than 5 micro m diameter. At the site of LRW discharge 80% of the discharged activity was adsorbed to these particles. Depending on the water flow, 40-80% of the suspensions precipitated at the bottom of subsequent sedimentation reservoirs. A total of about 1.6 MCi adsorbed to the suspended particles entered the open hydrographic system of the Techa river. The conclusion that the largest part of the activity was adsorbed on the suspended particles contradicts the assumption in the Techa river dosimetry system, TRDS-2000, that most of the released activity entered the Techa river in soluble form. For a correct reconstruction of the doses received by the Techa river population it is, therefore, essential to consider hydrodynamic models that take into account the transport of radionuclides adsorbed on the suspended particles.

Geologic Sediments↗

River habitat monitoring and assessment in Germany.

Whereas assessing the biological and chemical quality of water is a standard environmental procedure in many countries, the use of habitat survey methods that assess the ecomorphological quality of rivers is relatively new. In Europe, the EC Water Framework Directive requires such assessment from all EU Member States. In Germany, the first river habitat assessments were introduced in the late 1990 s. Each federal state develops its own river habitat map using the 'On-site Survey' and/or the 'Overview Survey'. The assessment describes the difference of the actual condition from a previously defined reference condition. In practice, a defined 'potential for restoration', a more realistic condition, makes restoration activities much easier and more successful. In Germany, the first River Habitat Map 2001 was published in 2002. The survey covered 33,000 km of river length, which equates to 10% of all rivers. A wide range from 'Undisturbed' (class 1) to 'Totally Disturbed' (class 7) river units exists; 77% of them are 'Clearly Disturbed' (class 4) or in worse condition. These result reflects extensive anthropogenic impact on the environment in general, but also past intense technical river 'improvements' that focused on the protection of settlements and traffic routes from flooding, better shipping conditions, the use of water power, and drainage of floodplains for agriculture and urban development. For comparability of survey results between EU Member States, a harmonization of national survey methods is in progress. A crucial point here is the definition of the reference condition for each river (near-natural conditions), since it influences the survey results.

Ecosystem↗

Environmental assessment of water-courses of the Turvo Limpo River basin at the Minas Gerais State, Brazil.

A study was performed to evaluate the environmental contamination in the Turvo Limpo River basin which receives effluent discharges from domestic (residential and commercial) activities. The watercourses examined were the São Bartolomeu Stream, Turvo Sujo River, and Turvo Limpo River, located in the Minas Gerais State, Brazil. Water samples were collected at the river-side and analyzed for evaluation of pollutant inputs. The pH, temperature, electrical conductivity, redox potential (Eh), dissolved oxygen (DO), total and settleable solids, visual color, hardness, chemical oxygen demand (COD), biochemical oxygen demand (BOD), chloride, total phosphate, total nitrogen, ammonia nitrogen, nitrate, total coliforms and E. coli, as well as the Cd, Pb, Cu, and Zn speciation were determined in the watercourses. The data obtained were compared with those of the Brazilian Environmental Standards and with data from non-contaminated areas. River water characteristics in some sites were far from the limit values established for superficial waters with satisfactory quality. For instance, the BOD values reached 411 mg L(-1) for a maximum limit of 10.0 mg L(-1), while the ammonia nitrogen concentration reached 28 mg L(-1) for a maximum limit of 13.3 mg L(-1). Some sites showed E. coli values above those of non-contaminated regions. Besides the effects of sewage discharges into the water-courses, agriculture activities and the use of the area for cattle husbandry influenced the quality of the river waters, for instance, the pH of a spring-water sample reached the value of 4.3. The São Bartolomeu Stream has been contributing to the deterioration of the water quality of the Turvo Sujo River, while the Turvo Limpo River has also been affected by anthropogenic discharges in the Turvo Sujo River. The speciation of Cd, Cu and Pb showed that these metals were mainly found in the particulate fraction (i. e., associated with the suspended material). Fifty five percent of the water samples showed labile Zn concentrations greater than that of the nonlabile Zn.

Brazil↗

Anthropogenic impacts on the distribution and biodiversity of benthic macroinvertebrates and water quality of the Langat River, Peninsular Malaysia.

A study of the impacts of anthropogenic activities on the distribution and biodiversity of benthic macroinvertebrates and water quality of the Langat River (Peninsular Malaysia) was conducted. Four pristine stations from the upstream and 4 stations at the downstream receiving anthropogenic impacts were selected along the river. For 4 consecutive months (March-June 1999), based on the Malaysian DOE (Malaysia Environmental Quality Report 2000, Department of Environment, Ministry of Science, Technology and Environment Malaysia. Maskha Sdn. Bhd. Kuala Lumpur, 86pp; Malaysia Environmental Quality Report 2001, Department of Environment, Ministry of Science, Technology and the Environment Malaysia) water quality index classes, the upstream stations recorded significantly (P<0.05) higher Biological Monitoring Working Party scores and better water quality indices than those of the downstream. The total number of macrobenthic taxa and their overall richness indices and diversity indices were significantly (P<0.05) higher at the upstream stations (54 taxa) than at the downstream stations (5 taxa). The upstream of the Langat River was dominated by Ephemeroptera and chironomid dipterans while other orders found in small quantities included Trichoptera, Diptera, Plecoptera, Odonata, Ephemeraptera, Coleoptera, and Gastropoda. On the other hand, the downstream of the river was mainly inhabited by the resistant Oligochaeta worms Limnodrilus spp. and Branchiodrilus sp. and Hirudinea in small numbers. The relationships between the physicochemical and the macrobenthic data were investigated by Pearson correlation analysis and multiple stepwise regression analysis. These statistical analyses showed that the richness and diversity indices were generally influenced by the total suspended solids and the conductivity of the river water. This study also highlighted the impacts of anthropogenic land-based activities such as urban runoff on the distribution and species diversity of macrobenthic invertebrates in the downstream of the Langat River. The data obtained in this study supported the use of the bioindicator concept for Malaysian rivers. Some sensitive (Trichopteran caddisflies and Ephemeraptera) and resistant species (Oligochaeta such as Limnodrilus spp.) are identified as potential bioindicators of clean and polluted river ecosystems, respectively, for Malaysian rivers.

Animals↗

Herbicide concentrations in the Mississippi River Basin-the importance of chloroacetanilide herbicide degradates.

The proportion of chloroacetanilide herbicide degradates, specifically the ethane sulfonic (ESA) and oxanilic (OA) acids, averaged 70% of the total herbicide concentration in samples from the Upper Mississippi River. In samples from the Missouri River and the Ohio River, the proportion of chloroacetanilide degradates in the total herbicide concentration was much less, 24% and 41%, respectively. The amount of tile drainage throughout the Mississippi River Basin appeared to be related to the occurrence and distribution of chloroacetanilide degradates in water samples. Pesticide concentrations in streams of the Mississippi River Basin have been well characterized. However, recent research demonstrates that in order to more fully understand the fate and transport of pesticides, the major pesticide degradates need to be included in the analysis. From March 1999 through May 2001, water samples from four major junctures of the Mississippi River Basin were collected and analyzed for a suite of herbicides and their degradate compounds. Each sampling site was selected to represent a major part of the Mississippi River: upper and lower Mississippi, Missouri and Ohio Rivers. Each basin has unique landscape variables, geology, hydrology, precipitation, and land use, which is reflected in the pesticide content at the most downstream sample site near the mouth of the Mississippi River. Atrazine was the most frequently detected herbicide (detected in 97% of the samples), followed by metolachlor (60%), and acetochlor (31%). The most frequently detected degradates were metolachlor ESA (69%), followed by deethylatrazine (62%), metolachlor OA (37%), and alachlor ESA (37%). Metolachlor ESA was detected more frequently than its parent compound (69 vs. 60%), as was alachlor ESA (37 vs. 9%). After an improvement was made in the analytical method, metolachlor ESA was detected in every sample, metolachlor OA in 89% of the samples, alachlor ESA in 84%, acetochlor ESA in 71%, and acetochlor OA in 66%.

Acetamides↗

Impact of a highly contaminated river on a more important hydrologic system: changes in organic markers.

The Fensch River is a tributary of the Moselle River. It is a highly contaminated river that drains an industrial area. The objective of this preliminary study is to determine its impact on the Moselle River by analysing the extractable organic matter (EOM) coming from the sediments at the molecular scale. EOM is described in term of aromatic hydrocarbons, aliphatic hydrocarbons and polar compounds. EOM coming from Fensch River sediments is mainly composed of anthropogenic molecules. Aromatic hydrocarbons are dominated by parent Polycyclic Aromatic Hydrocarbons (PAHs) underlining the pyrogenic origin of this fraction. Aliphatic hydrocarbons consist of diagenetic hopanes and a broad UCM that are characteristics of thermal mature organic matter. Upstream the confluence the EOM of the Moselle River is mainly from vegetal origin. It is composed of high molecular weight n-alkanes with an odd over even predominance, degredation products of phytol and stigmasterol. The occurrence of PAHs and diagenetic hopanes underlines that the Moselle River is already contaminated before the confluence. The Fensch River input drastically changes the EOM of the Moselle River. Amount of PAHs is doubled and the fingerprints of both aliphatic hydrocarbons and polar compounds highlight the combination of both natural and anthropogenic sources.

Coal Tar↗

Design of a monitoring network and assessment of the pollution on the Lerma river and its tributaries by wastewaters disposal.

While the 2005 progress report of the United Nations Millennium Development Goals stresses out the need of a dramatic increase in investment to meet the sanitation target in the third world, it is important to anticipate about some parallel negative impacts that may have this optimistic programme (extension of sewer networks without sufficient treatment works). Research was initiated on Lerma River (Mexico), subjected to many rejects disposal, to design a monitoring network and evaluate the impact of wastewaters on its water quality. The discharges was inventorized, geo-positioned with a GPS and mapped, while the physico-chemical characteristics of the river water, its tributaries and main rejects were evaluated. Microtox system was used as an additional screening tool. Along the 60 km of the High Course of Lerma River (HCLR), 51 discharges, with a diameter or width larger than 0.3 m (including 7 small tributaries) were identified. Based on the inventory, a monitoring network of 21 sampling stations in the river and 13 in the important discharges (>2 m) was proposed. A great similitude was found between the average characteristics of the discharges and the river itself, in both the wet and dry seasons. Oxygen was found exhausted (<0.5 mg/L) almost all along the high course of the river, with COD and TDS average levels of 390 and 1980 mg/L in the dry season, against 150 and 400 mg/L in the wet season. In the dry season, almost all the sites along the river revealed some toxicity to the bacteria test species (2.9 to 150 TU, with an average of 27 TU). Same septic conditions and toxicity levels were observed in many of the discharges. Four of the six evaluated tributaries, as well as the lagoon (origin of the river), were relatively in better conditions (2 to 8 mg/L D.O., TU<1) than for the Lerma, acting as diluents and renewal of the HCLR flow rate. The river was shown to be quite a main sewer collector. The high surface water contamination by untreated wastewaters that is depicted in this research should be taken into account in the Millennium Goals strategies, by promoting treatment plan works simultaneously, when sewer networks in the third world would extend.

Aliivibrio fischeri↗

A molecular phylogeny of aquatic gastropods provides a new perspective on biogeographic history of the Snake River Region.

Mitochondrial DNA sequences of aquatic gastropods of the subgenus Pyrgulopsis (Natricola) were analyzed to test a commonly accepted hypothesis concerning the early history of the Snake River in the northwestern US. Distributions of Natricola and other regional biota were previously used to infer that the Snake River flowed to the Pacific through southeastern Oregon and northern California during the Neogene prior to its capture by the Columbia River in the late Pliocene (2 Ma). A molecular phylogeny based on partial sequences of COI and NDI (1149 bp) indicates that the Natricola clade is restricted to the modern Snake-Columbia River Basin and the Oregon Lakes region whereas northern California populations previously assigned to this subgenus belong to other lineages. The Natricola clade is not deeply subdivided into Oregon Lakes and Snake River Basin units consistent with late Pliocene fragmentation of the hypothesized paleodrainage, but instead is shallowly structured and contains multiple transitions among these two geographic areas. The strongly supported sister relationship between Natricola and a species from northwest Nevada (P. imperialis) is consistent with a recent proposal that the ancestral Snake River did not flow through southeast Oregon but instead flowed south to the Humboldt River. Within the context of this hypothesis, the multiple transitions between the Snake River Basin and the Oregon Lakes region that occurred within Natricola may be attributed to a late Pleistocene connection between these areas that was unrelated to the early course of the Snake River.

Animals↗

Transport of suspended-sediment-bound toxaphene in the Mississippi River.

Suspended sediment samples from approximately 30 locations along the Mississippi River and six of its major tributaries were collected during July and August 2002 and March 2003 to investigate the distribution and transport of toxaphene in the Mississippi River. The concentration of toxaphene was measured, and the load of toxaphene carried by the river at each site was calculated using water discharge and suspended sediment concentrations. Results indicate that toxaphene is widespread throughout the Mississippi River Basin with the highest concentrations and loads observed for samples collected in the Lower Mississippi River. Among the tributaries, the Yazoo River carried the largest load, amounting to approximately 21% of the estimated load carried by the Mississippi River just below Vicksburg, MS. The majority of the contamination is most likely from nonpoint source runoff from agricultural lands. We also found evidence of a localized toxaphene source in the vicinity of Memphis, perhaps coming from the Wolf River. From our data we estimate that between 200 and 1000 kg of toxaphene were released into the Gulf of Mexico in 2002 from the main stem of the Mississippi River.

Agriculture↗

Response of sulfate concentration and isotope composition in Icelandic rivers to the decline in global atmospheric SO2 emissions into the North Atlantic Region.

This study presents the changes in dissolved sulfate concentration and isotope composition of Icelandic river waters between the peak of SO2 emissions in the United States and Europe and the present. Chloride concentration in Icelandic rivers has not changed much since 1972. The overall average change from 1972-1973 to 1996-2004 was -3%, indicating insignificant sea-salt contribution changes. More than 99% of the river-dissolved sulfur was in the form of sulfate. There are three main sources for dissolved sulfate in the rivers: rocks, sea-salts, and anthropogenic. Total dissolved sulfate, tdSO4(2-), and non-sea-salt sulfate, nssSO4(2-), decreased in all of the rivers from the early 1970s to 1996-2004. The percentage decrease varies from 13% to 65%. The decrease is smallest in rivers were there is considerable rock-derived dissolved SO4(2-). The overall average decrease was 39% for tdSO4(2-) and 46% for nssSO4(2-). The anthropogenic sulfate fraction has declined making most of the river waters delta34S values of sulfate higherthrough time. The overall decline in river sulfate and increase in delta34S, while SO2 emissions from Iceland has been increasing, demonstrates the response of river chemistry in the remote North Atlantic to the decline in man-made emissions of SO2 in North America and Europe.

Air Pollutants↗

Occurrence and behaviour of 4-nonylphenol in river water of Germany.

An analytical method composed of solid-phase-extraction (SPE) followed by gas chromatography-mass spectrometry (GC-MS) has been developed to investigate the occurrence and the behaviour of the endocrine disruptor 4-nonylphenol (4-NP) in river water of Germany. 16 Para-isomers of the complex mixture of 4-NP isomers have been resolved in the SPE-extracts of river water samples. The mass spectra indicate the presence of four distinct groups of isomers. The different groups are characterised by different base peaks of the mass spectra dependent on the substitution of the alpha-carbon on the alkyl chain. The different isomers show (i) alpha-methyl, alpha-ethyl (base peak 149), (ii) alpha-dimethyl (base peak 135), (iii) alpha-methyl, alpha-propyl, alpha-butyl (base peak 121) and (iv) alpha-methyl, alpha-propyl, alpha-butyl (base peak 107) configuration of the alkyl chain. The recovery of the unbranched compound 4-n-NP was > or = 90%. Detection limit and determination limit of the whole analytical procedure were 6 ng L(-1) and 25 ng L(-1), respectively with a relative standard deviation of 11.7%. The concentration of the sum of the 4-NP isomers in all samples of river water ranged from 28 ng L(-1) (Elbe River) to 1220 ng L(-1)(Oder River). In a period of a higher river water level, a higher amount of 4-NP was detected in the Oder River than in a period of a lower water level. Therefore dilution was not the determinant factor of the 4-NP concentration in river water. The diurnal dynamics of the sewage treatment works (STWs) have to be considered as an influencing factor for the presence of 4-NP in river water.

Environmental Monitoring↗

Modeling possible cooling-water intake system impacts on Ohio River fish populations.

To assess the possible impacts caused by cooling-water intake system entrainment and impingement losses, populations of six target fish species near power plants on the Ohio River were modeled. A Leslie matrix model was constructed to allow an evaluation of bluegill, freshwater drum, emerald shiner, gizzard shad, sauger, and white bass populations within five river pools. Site-specific information on fish abundance and length-frequency distribution was obtained from long-term Ohio River Ecological Research Program and Ohio River Sanitation Commission (ORSANCO) electrofishing monitoring programs. Entrainment and impingement data were obtained from 316(b) demonstrations previously completed at eight Ohio River power plants. The model was first run under a scenario representative of current conditions, which included fish losses due to entrainment and impingement. The model was then rerun with these losses added back into the populations, representative of what would happen if all entrainment and impingement losses were eliminated. The model was run to represent a 50-year time period, which is a typical life span for an Ohio River coal-fired power plant. Percent changes between populations modeled with and without entrainment and impingement losses in each pool were compared to the mean interannual coefficient of variation (CV), a measure of normal fish population variability. In 6 of the 22 scenarios of fish species and river pools that were evaluated (6 species x 5 river pools, minus 8 species/river pool combinations that could not be evaluated due to insufficient fish data), the projected fish population change was greater than the expected variability of the existing fish population, indicating a possible adverse environmental impact. Given the number of other variables affecting fish populations and the conservative modeling approach, which assumed 100% mortality for all entrained fish and eggs, it was concluded that the likelihood of impact was by no means assured, even in these six cases. It was concluded that in most cases, current entrainment and impingement losses at six Ohio River power plants have little or no effect at the population level.

Animals↗

Quantifying ground water inputs along the Lower Jordan River.

The flow rate of the Lower Jordan River has changed dramatically during the second half of the 20th century. The diversion of its major natural sources reduced its flow rate and led to drying events during the drought years of 2000 and 2001. Under these conditions of low flow rates, the potential influence of external sources on the river discharge and chemical composition became significant. Our measurements show that the concentrations of chloride, calcium, and sodium in the river water decrease along the first 20-km section, while sulfate and magnesium concentrations increase. These variations were addressed by a recent geochemical study, suggesting that ground water inflow plays a major role. To further examine the role of ground water, we applied mass-balance calculations, using detailed flow rate measurements, water samplings, and chemical analyses along the northern (upstream) part of the river. Our flow-rate measurements showed that the river base-flow during 2000 and 2001 was 500 to 1100 L s(-1), which is about 40 times lower than the historical flow rates. Our measurements and calculations indicate that ground water input was 20 to 80% of the river water flow, and 20 to 50% of its solute mass flow. This study independently identifies the composition of possible end-members. These end-members contain high sulfate concentration and have similar chemical characteristics as were found in agricultural drains and in the "saline" Yarmouk River. Future regional development plans that include the river flow rate and chemistry should consider the interactions between the river and its shallow ground water system.

Environmental Monitoring↗

Temporal and spatial distributions of sediment total organic carbon in an estuary river.

Understanding temporal and spatial distributions of naturally occurring total organic carbon (TOC) in sediments is critical because TOC is an important feature of surface water quality. This study investigated temporal and spatial distributions of sediment TOC and its relationships to sediment contaminants in the Cedar and Ortega Rivers, Florida, USA, using three-dimensional kriging analysis and field measurement. Analysis of field data showed that large temporal changes in sediment TOC concentrations occurred in the rivers, which reflected changes in the characteristics and magnitude of inputs into the rivers during approximately the last 100 yr. The average concentration of TOC in sediments from the Cedar and Ortega Rivers was 12.7% with a maximum of 22.6% and a minimum of 2.3%. In general, more TOC accumulated at the upper 1.0 m of the sediment in the southern part of the Ortega River although the TOC sedimentation varied with locations and depths. In contrast, high concentrations of sediment contaminants, that is, total polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), were found in sediments from the Cedar River. There was no correlation between TOC and PAHs or PCBs in these river sediments. This finding is in contradiction to some other studies which reported that the sorption of hydrocarbons is highly related to the organic matter content of sediments. This discrepancy occurred because of the differences in TOC and hydrocarbon source input locations. It was found that more TOC loaded into the southern part of the Ortega River, while almost all of the hydrocarbons entered into the Cedar River. This study suggested that the locations of their input sources as well as the land use patterns should also be considered when relating hydrocarbons to sediment TOC.

Carbon↗

Considerations on the influence of extreme events on the phosphorus transport from river catchments to the sea.

In this paper, results from rivers of different sizes in Romania, Hungary and Austria are presented. The paper shows the dynamics of extreme events and their contribution to the total P and suspended solids transported in these rivers. Special attention is paid to the influence of the size of the catchment and the event probability on the relative contribution of a single event to the total loads transported in the river. Further, the development of phosphorus loads along the Danube River at a flood event is shown. From the results it can be concluded that there is no immediate influence of high flow and flood events in upstream parts of the Basin on the transport of phosphorus from the catchment to the receiving Sea. Particle-bound phosphorus is mobilised from the catchment (through erosion) and the river bottom to a high extent at high flow events and transported at peak discharges to downstream, where retention by sedimentation of particles takes place. On the one hand this retention is a transport to flooded areas. In this case it can be considered as more or less long term retention. On the other hand sedimentation takes place in the riverbed, in case the tractive effort of the river is reduced. In this second case the P-pool in the sediments of the sedimentation area will be increased. If anaerobic conditions in the sediment appear, part of the phosphorus will be transformed to soluble ortho-phosphate and will continuously contribute to the phosphorus transport to the receiving sea. Part of the P-retained in the river sediment will be mobilised by resuspension at the next biggest high flow event. Altogether, these alternating processes of suspension, transport, export to flooded areas or sedimentation in the river bed with partly solution and partly resuspension at the next event decrease the share of the phosphorus transport during high flow events on the total loads transported in the more downstream parts of a catchments as compared to the more upstream parts. In the year of occurrence of an extreme flood event the P-transport of this year is dominated by the flood event. As an average over many years the contribution of high flow events to the total P-transport still may be between 7 and 20% in smaller catchments (around 1,000 km2). In a big catchment (e.g. river Danube) much smaller contributions of flood events on the total P-transport can be expected as an average over many years.

Austria↗

[Calculation of environmental flows in river reaches based on ecological objectives].

Based on the identifying the ecological objectives, environmental flows in river reaches is calculated after the relation between parameters of objectives, and river discharges is determined. The ecological objectives are determined in a two step process: theobjective will be determined for the critical period of the year, then the temporal variation will be defined. Considering the compatibility between the different kinds of environmental flow requirements, the strictest objective is settled to be the ecological objectives for the critical period of the year. The temporal variation of the natural river discharge monthly is settled to be the temporal variation of ecological objectives. In the studies of the environmental flows in the river reaches downstream for Guanting reservoir in the Yongding River of Haihe River Basin, the requirements of velocities for spawning of the fish in April are regarded as the ecological objectives in the most critical period because this also is the period of highest water demand for irrigation. The relation between objectives and river discharge is identified using historical data at the river station. The results indicate that the minimum, medium and ideal lever of annual environmental flow requirements are 1.56 x 10(8) m3, 5.97 x 10(8) m3 and 11.02 x 10(8) m3, about 7.19%, 27.51% and 50.78 % of the natural river discharge respectively. The ratio of water requirements monthly should be 20 % in the flood period (Aug.) and be 20% in biological propagation period in spring (Apr. - Jun.).

Conservation of Natural Resources↗

Toxicity evaluation of waters from a tributary of the River Po using the 7-Day Ceriodaphnia dubia test.

Seven-day toxicity tests with Ceriodaphnia dubia were used to assess ambient water toxicity in the River Lambro, a tributary of the River Po, and in the corresponding stretch of the main river located downstream from their confluence. About once a month, toxicity tests were conducted on water samples of the River Lambro, using as dilution water the water of the main river collected upstream from the confluence. With a lower frequency, the downstream stretch of the River Po was tested for ambient toxicity at four sites located at 6, 11, 16, and 21 km from the emission of the tributary. The River Lambro demonstrated variable toxicity in different time periods, although the most frequent effects were sublethal, on both reproduction and growth. Ammonia, nickel, and zinc can be indicated as possible toxicants. Acute toxic effects to C. dubia were observed with spring samples only, when the action of some pesticides, likely insecticides, have to be taken into account. Tests conducted on downstream water gave limited results, seemingly because the dilution capacity of the River Po reduced Lambro toxicity to a level close to and often below the detection limit of the 7-day test. Accordingly, toxic effects at downstream sites could only be observed on the growth of C. dubia, which was found to be the most sensitive endpoint. Fairly good agreement was found between predictions based on toxicity tests of the River Lambro and the effects observed for downstream samples.

Ammonia↗