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PubMed1986

[Polish standard for anti-Brucella abortus serum for agglutination as the basis for the standardization of the antigen for the rose bengal plate test].

The aim of the study was to test out the usability of Polish Standard of anti-Brucella abortus serum in agglutination for standardization and control of antigen commercial manufacturing. Polish Standard of anti-Brucella abortus serum in agglutination tube test (PSaBaS-SAT) was separated on Sefadeks G-200 gel to determine the content of anti-Brucella antibodies of IgM class, characteristic of the first stage of infection, and IgG antibodies typical for chronic disease. Serological examinations of the fractions obtained, revealed significant domination of specific IgG antibodies over IgM. Visible activity of IgG in RBPT (Rose Bengal Plate Test) and in other serological test used in Poland to recognize brucellosis, was observed. It enables us to use PSaBaS-SAT in the determination of agglutinability of acid antigen as the antibodies of this class are commonly recognized to be a specific indicator of active brucellosis on the contrary to IgM which may be of non specific character. It is suggested that additional testing of agglutinability of acidic antigen, compared to IgM antibodies is not necessary and increases the cost of studies. Moreover, the examination of animals twice a year, using RBPT gives slight probability to find animals in their first stage of infection (high level of IgM in serum) as the only ones in the population studied.

Agglutination Tests↗