The diagnosis of ringworm infection in small animals.
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A survey of 568 horses in training and 2,535 horses on breeding farms has shown 32 percent of the horses in training to be clinically affected while only 1.1 percent of breeding horses were affected with pathogenic dermatophytes. The majority of lesions on racing horses were located on the girth areas. Trichophyton equinum var. autotrophicum, M. canis and M. equinum were found to be restricted to racing horses only. M. gypseum occurred in racing, riding and breeding horses.
The epidemiology of dermatomycocis due to Trichophyton equinum var autotrophicum was studied in a number of thoroughbred stables in south-east Queensland. The significant factors in the epidemiology were defined. The infection was readily transmitted, particularly by infected saddle-girths, on which the fungus could survive for 12 months. Mild abrasion from the saddle during work favoured the development of lesions and prolonged the recovery period. A pronounced age and seasonal incidence of the disease was demonstrated as young horses under the age of 3 years were most susceptible, and the majority of cases occurred in periods of high humidity.
We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of the vaccine strain of the Czech live vaccine Bioveta. Comparative investigations were carried out with the vaccine strain of the Russian live vaccine LTF 130 and Trichophyton verrucosum field strains. Growth of the vaccine strains was faster than growth of the field strains on solid media and in broth. Later the vaccine strains formed many microconidia. In experimentally infected guinea pigs the virulence of the vaccine strain Bioveta was highly dependent on the infectious dose. At 10(1) CFU ml-1 (according to the number of CFUs in the Russian live vaccine) the vaccine strain Bioveta was nearly avirulent. Resuspended according to the manufacturer's instructions (at least 10(10) CFU ml-1) virulence of the vaccine strain Bioveta could be observed.
Isolants of nine species of Trichophyton, one of Epidermophyton, and four of Microsporum were assayed for elastase activity. The species or isolants with elastase activity were obtained from patients with inflammatory ring-worm infection. In Nannizzia fulva (M. fulvum), plus-mating-type strains were elastase-positive and minus-mating-type strains elastase-negative. A genetic study of mating type and elastase activity indicated a monogenic basis for both mating type and elastase activity.
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