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Reproductive isolation between two closely related hummingbird-pollinated neotropical gingers.

Empirical estimates of the relative importance of different barriers to gene flow between recently diverged species are important for understanding processes of speciation. I investigated the factors contributing to reproductive isolation between Costus pulverulentus and C. scaber (Costaceae), two closely related hummingbird-pollinated understory Neotropical herbs. I studied broad-scale geographic isolation, microhabitat isolation, flowering phenology, overlap in pollinator assemblages, floral constancy by pollinators, mechanical floral isolation, pollen-pistil interactions, seed set in interspecific crosses, and postzygotic isolation (hybrid seed germination, greenhouse survival to flowering, and pollen fertility). Aside from substantial geographic isolation, I found evidence for several factors contributing to reproductive isolation in the sympatric portion of their geographic ranges, but the identity and relative strength of these factors varied depending on the direction of potential gene flow. For C. pulverulentus as the maternal parent, mechanical floral isolation was the most important factor, acting as a complete block to interspecific pollen deposition. For C. scaber as the maternal parent, microhabitat isolation, pollinator assemblage, mechanical floral isolation, and postpollination pollen-pistil incompatibility were important. Overall, prezygotic barriers were found to be strong, resulting in 100% reproductive isolation for C. pulverulentus as the maternal parent and 99.0% reproductive isolation for C. scaber as the maternal parent. Some postzygotic isolation also was identified in the F1 generation, increasing total isolation for C. scaber to 99.4%. The results suggest that ecological factors, including habitat use and plant-pollinator interactions, contributed to speciation in this system and evolved before extensive intrinsic postzygotic isolation.

Animals↗

Experimental infection of the neotropical malaria vector Anopheles darlingi by human patient-derived Plasmodium vivax in the Peruvian Amazon.

Malaria transmission from humans to mosquitoes is modulated by human host immune factors. Understanding mechanisms by which the human host response may impair parasite infectivity for mosquitoes has direct implications for the development of transmission-blocking vaccines. We hypothesized that despite a low transmission intensity of malaria in the Peruvian Amazon region of Iquitos, transmission-blocking immunity against Plasmodium vivax might be common, given an unexpectedly high proportion of asymptomatic parasitemic individuals in this region. To test this hypothesis, the ability of symptomatic P. vivax malaria patients to experimentally infect wild-caught outbred Anopheles darlingi mosquitoes was tested using the indirect membrane feeding technique. Only half (52/102) of P. vivax parasitemic patients successfully infected mosquitoes. Transmitters were more likely to have gametocytes (OR 6.35, P = 0.003), high parasitemia (OR 3.79, P = 0.024), and, in terms of basic clinical parameters, a slower pulse rate (mean +/- SD: 82.3 +/- 12.3 versus 88.7 +/- 13.5, P = 0.016) than non-transmitters. Log(10) gametocytemia and log(10) real-time reverse transcriptase Pvs25 PCR quantifying gametocytes were significantly and positively correlated with oocyst counts (correlation coefficient 0.505, R2 = 0.26, P = 0.001). These experiments are the first to establish a system of determining transmission patterns in experimental infection of outbred natural neotropical malaria vectors in the Amazon region. Patients with P. vivax inefficiently infect outbred An. darlingi mosquitoes, raising the possibility that some degree of naturally occurring transmission-blocking immunity is present on a population basis in the Peruvian Amazon, an area of low intensity of malaria transmission.

Adolescent↗

Aphid derived sugars in the neotropical sandfly--Lutzomyia peruensis.

It is known that a sugar meal is a prerequisite for the transmission of Leishmania by sandflies in the laboratory. Lutzomyia peruensis the proven vector of Leishmania peruviana, was caught by aspiration from crevices in rocks near Chaute in the Rimac Valley, Peru, cryopreserved and analysed for sugars using HPLC. The major sugars present are glucose and fructose as well as smaller amounts of sucrose, maltose, melibiose, turanose and a trisaccharide, probably raffinose. The results indicate that the major carbohydrate sugar meal of Lutzomyia peruensis is aphid honeydew. This is the first report of such behaviour in Neotropical sandflies.

Animals↗

Proteocephalus brooksi n. sp. (Cestoda:Proteocephalidae) in the neotropical freshwater fish Rhamdia guatemalensis (Siluriformes: Pimelodidae) from Lake Catemaco, Veracruz, Mexico.

Proteocephalus brooksi n. sp. is described from the neotropical pimelodid fish Rhamdia guatemalensis from Lake Catemaco, Veracruz, México. The new species is characterized by the "paramuscular" position of vitellaria, the cortical origin of uterine stem with development of medullar lateral branches, the alternated position of vagina (anteriorly and posteriorly to cirrus sac), and the absence of apical organ and vaginal sphincter. Proteocephalus brooksi most closely resembles Nomimoscolex matogrossensis from which it differs in a series of characters. The probable paraphyletic nature of both Proteocephalus and Nomimoscolex and the convergent evolution of the "paramuscular" location of vitellaria among proteocephalideans are suggested, and the need for a phylogenetic analysis of the group is emphasized. The new species is assigned to Proteocephalus pending such an analysis.

Animals↗

Egg maturation in neotropical malaria vectors: one blood meal is usually enough.

The requirement of more than one blood meal to complete a gonotrophic cycle is reported here to be infrequent among field-collected Anopheles (Nyssorhynchus), including Anopheles darlingi, South America's most important malaria vector. The overall frequencies of host-seeking females that did not develop eggs after one blood feeding were low for Anopheles darlingi (6.6%), Anopheles rondoni (5.0%), and Anopheles triannulatus (2.2%); the majority of wild-caught females that did not develop eggs after one blood meal were nulliparous. Laboratory-reared Anopheles albimanus and Anopheles albitarsis, other species of the same subgenus, were grown on enriched and impoverished larval diets to yield a range of adult sizes and to examine relationships between egg maturation, body size, and blood meal intake. 10.7% of nulliparous An. albimanus and 22.9% of An. albitarsis failed to mature eggs after sugar and one blood meal, and shorter wings and smaller blood meals predisposed females to require multiple blood feeds for egg maturation. Unmated An. albitarsis were significantly more likely than mated females not to develop eggs. Multiple blood meals within a single gonotrophic cycle appear to be less important in the life histories of neotropical Nyssorhynchus species compared to afrotropical malaria vectors.

Animals↗

Behavioral thermoregulation in a sexually and developmentally dichromatic neotropical primate, the black-and-gold howling monkey (Alouatta caraya).

Behavioral thermoregulation in primates may provide a means for the conservation of heat during periods of low ambient temperature and/or food shortage as well as a way to dissipate heat under hot conditions. This article focuses on behavioral thermoregulation in a sexually dichromatic primate, the black-and-gold howling monkey (Alouatta caraya). Two models have been proposed to explain the evolution of sexual dichromatism in this species: thermoregulation and sexual selection. Five hypotheses associated with thermoregulatory behaviors are tested. These are as follows: (1) energy-conserving postures are used mainly under low ambient temperatures; (2) sunny resting places are selected during periods of low temperature; (3) exposure of the less-insulated ventral region to sunlight decreases with increasing temperature; (4) black-colored adult males use energy-conserving postures, sunny places, and exposure of the ventral region to sunlight less frequently than do blonde-colored adult females; and (5) smaller individuals use energy-conserving postures, sunny places, and exposure of the ventral region to sunlight in significantly greater frequency than do larger individuals. Over a 12-month period, behavioral data were collected on a free-ranging habituated group of 15-17 howlers of all age-sex classes. Ambient temperature was measured each hour. The results indicate that during resting, howlers showed a consistent use of heat-conserving postures, showed a preference for sunny places, and exposed their ventral region to sunlight under low ambient temperatures. A preference for shady places, heat-dissipating postures, and exposure of the back were observed under high ambient temperatures. Despite sex differences in adult color patterns and differences in size between age classes, no significant age or sex differences in thermoregulatory behaviors were detected. Failure to confirm a thermoregulation model implies that sexual selection may be responsible for sexual dichromatism in this species.

Alouatta↗

Structure and morphogenesis of the uterus, placenta, and paraplacental organs of the neotropical disc-winged bat Thyroptera tricolor spix (Microchiroptera: Thyropteridae).

The structure and development of the female reproductive tract, fetal membranes and placental have not previously been recorded for any member of the family Thyropteridae. Recently implanted embryos were obtained in late January, limb-bud stages in March, and full-term fetuses in late May, suggesting a possible gestation length of approximately five months. It is likely, however, that Thyroptera experiences at least two breeding cycles per year. The uterus was narrowly bicornuate; the corpus uteri was unusually large and lacked the glandular density observed in the cornua. The cervix was long, pleated, and relatively aglandular. The oviducts opened at the apices of the cornua; oviductal papillae were absent. A bursa ovarii surrounded the ovary, but there was a small pore opening to the peritoneal cavity adjacent to the fimbriated end of the oviduct. Never more than a single embryo or fetus was present, and only a single corpus luteum was observed; thus Thyroptera, like most bats, is monovular. Ovoimplantation was interstitial; a decidua capsularis was present early but disappeared by the late limb-bud stage. The decidual reaction involved both glandular epithelium and stromal cells, but most of the decidua was destroyed by term. Amniogenesis was initiated after implantation, by cavitation. Primitive entoderm was formed precociously above, as well as below, the presumptive embryonic disc, and a thin extension of Reichert's membrane passed over the cell mass, separating it from the cytotrophoblast of the chorionic placenta. During the amniogenic period, the yolk-sac entoderm fused to the parietal trophoblast via an intervening Reichert's membrane, forming an extensive bilaminar omphalopleure; this was rapidly converted to a trilaminar structure in early post-implantation stages. An avascular chorio-vitelline relationship involved most of the chorionic wall in early post-implantation stages and persisted to term in the abembryonic hemisphere after the partial inversion of the yolk-sac roof in late presomite embryos. The invaginated yolk-sac roof (splanchnopleure) also persisted to term as a viable paraplacental component. A small sac-like allantois was formed between late presomite and early limb-bud stages but disappeared by the late limb-bud stage. Development of the definitive chorioallantoic placenta resembled that in other bats, but the maternal endothelium disappeared relatively early, and trophoblastic differentiation was precocious. The ultrastructural organization of the interhemal membrane was hemodichorial, and otherwise generally resembled the organization previously described in vespertilionid bats. Similarities and differences in the structure of the uterus, placenta, and paraplacental organs of Thyropteridae, in comparison with other families of bats, are discussed. On the basis of fetal membrane characteristics, the Thyropteridae show closer affinities with the Phyllostomatoidea than with the Vespertilionoidea, to which they are presently assigned.

Allantois↗

Retinofugal projections of the big brown bat, Eptesicus fuscus and the neotropical fruit bat, Artibeus jamaicensis.

Retinal connections were studied in Eptesicus fuscus and Artibeus jamaicensis using anterograde axonal degeneration and autoradiographic techniques following unilateral enucleations and uniocular injections of radioactive amino acids. Although each retina projected bilaterally to the brainstem, the number of silver grains in the emulsion of autoradiographs indicated that nearly all fibers in the optic nerve entered the contralateral optic tract. Ipsilaterally, a major portion of the projection ended in the suprachiasmatic nucleus; caudal to the suprachiasmatic nucleus, the amount of label was so small that individual silver grains were counted to determine the location and quantity of label in other ipsilateral nuclei. In both species the retinal projection terminated bilaterally in the suprachiasmatic, dorsal lateral geniculate, ventral lateral geniculate, and pretectal olivary nuclei and contralaterally in the posterior pretectal nucleus, superficial gray layers of the superior colliculus, and nuclei of the accessory optic system. In Eptesicus the projection to the nucleus of the optic tract ended contralaterally, and in Artibeus it ended in this nucleus bilaterally. The results of this study revealed a basic theme in the optic projection of the two ecologically different microchiropterans. The results differed, however, in that the projection was larger and visually related nuclei were better developed in Artibeus. Such variations are presumed to relate to eye size and the relative use of vision by the two chiropterans.

Animals↗

Multivariate analysis of organismal and habitat parameters in two neotropical primate communities.

This paper investigates the interrelations of some organismal and habitat parameters in two platyrrhine primate communities in French Guiana and Ecuadorian Amazonia. Fifteen habitat and organismal parameters and body weight were retained after a preliminary analysis, on the basis of frequent use and constraints reflected in anatomical specializations. The 16 parameters for 13 platyrrhine species were used for a series of principal components analyses (PCAs). All PCAs on separate or combined communities provided comparable results. The Atelinae, Ateles, Alouatta, and Lagothrix, were clustered together with Pithecia, associated with canopy, mature forest, frugivory, and (to a lesser degree) climb/suspensory locomotion. On the other hand, the three species of Cebus usually formed a loose cluster, located in the center of the cloud of species. Lastly, the Callitrichinae, involving two species of Saguinus and Cebuella, were relatively dispersed and frequently associated with liana forest, lianas, understory, and faunivory. However, Cebuella was consistently isolated and associated with liana forest, lianas, body weight, understory, and vertical supports. In this way, clusters appeared to conform to the major platyrrhine taxonomic groups, supporting the fact that basic ecological and behavioral adaptations have evolved in a phylogenetic context. In addition, the analyses revealed that body weight, forest type and layer, feeding behavior, and locomotion are the most important variables that have apparently shaped or driven the adaptive zones of extant platyrrhines.

Alouatta↗

Assessing parental effort in a Neotropical parrot: a comparison of methods.

Various behavioural or body condition indices are frequently used to measure avian parental effort. To determine which of the indices provide meaningful short-term assessments of parental effort, we used four of them concurrently on breeding green-rumped parrotlets, Forpus passerinus, and then compared the results with field metabolic rate (FMR) measurements obtained using the doubly labelled water (DLW) technique. Nest provisioning rate correlated significantly with FMR among both males and females, while proportion of time away from the nest was correlated with FMR in females but not in males. Adult mass change during the breeding cycle and feeding latency were both unrelated to FMR. Our results suggest researchers should be cautious in assuming that any indirect method of quantifying parental effort is reliable for a particular species. We also tested whether the relatively invasive DLW protocol causes potentially confounding changes in study animals' subsequent behaviour. Paired observations before and after DLW injection showed no measurable changes in parrotlet nest attendance patterns or nest provisioning rate. Copyright 1999 The Association for the Study of Animal Behaviour.

Journal Article↗

Acoustic interference limits call detection in a Neotropical frog Hyla ebraccata.

Problems1999 The Association for the Study of Animal Behaviourassociated with communication in noisy environments include detection, discrimination, and localization of appropriate signals. I investigated the effects of broadband background noise on call detection by female Copyright 1999 The Association for the Study of Animal Behaviour.

Journal Article↗

Horizontal transmission of Amblyospora albifasciati García and Becnel, 1994 (Microsporidia: amblyosporidae), to a copepod intermediate host and the neotropical mosquito, Aedes albifasciatus (Macquart, 1837).

The life cycle of Amblyospora albifasciati is characterized by three sporulation sequences involving the definitive mosquito host and a copepod intermediate host. Meiospores of A. albifasciati were infectious per os to adult females of the copepod Mesocyclops annulatus. All developmental stages in the copepod had unpaired nuclei, with sporulation involving the formation of a sporontogenic interfacial envelope and the production of a second type of uninucleate spore. These spores, formed in the ovaries of M. annulatus, were large, pyriform, and measured 10.4 x 4.8 microm. They infected Aedes albifasciatus larvae when ingested to initiate a sequence that involves schizogony and gametogony and ends with plasmogamy and nuclear association to form diplokaryotic meronts. Oval binucleate spores (9.3 x 3.1 microm) are formed in the adult mosquito and are responsible for vertical transmission to the filial generation.

Aedes↗