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Chemical and physical changes following co-composting of beef cattle feedlot manure with phosphogypsum.

Nitrogen (N) loss during beef cattle (Bos taurus) feedlot manure composting may contribute to greenhouse gas emissions and increase ammonia (NH(3)) in the atmosphere while decreasing the fertilizer value of the final compost. Phosphogypsum (PG) is an acidic by-product of phosphorus (P) fertilizer manufacture and large stockpiles currently exist in Alberta. This experiment examined co-composting of PG (at rates of 0, 40, 70, and 140 kg PG Mg(-1) manure plus PG dry weight) with manure from feedlot pens bedded with straw or wood chips. During the 99-d composting period, PG addition reduced total nitrogen (TN) loss by 0.11% for each 1 kg Mg(-1) increment in PG rate. Available N at the end of composting was significantly higher for wood chip-bedded (2180 mg kg(-1)) than straw-bedded manure treatments (1820 mg kg(-1)). Total sulfur (TS) concentration in the final compost increased by 0.19 g kg(-1) for each 1 kg Mg(-1) increment in PG rate from 5.2 g TS kg(-1) without PG addition. Phosphogypsum (1.6 g kg(-1) P) addition had no significant effect on total phosphorus (TP) concentration of the final composts. Results from this study demonstrate the potential of PG addition to reduce overall N losses during composting. The accompanying increase in TS content has implications for use of the end-product on sulfur-deficient soils. Co-composting feedlot manure with PG may provide an inexpensive and technologically straightforward solution for managing and improving the nutrient composition of composted cattle manure.

Alberta↗

Salmonella survival in manure-treated soils during simulated seasonal temperature exposure.

Addition of animal manure to soil can provide opportunity for Salmonella contamination of soil, water, and food. This study examined how exposure of hog manure-treated loamy sand and clay soils to different simulated seasonal temperature sequences influenced the length of Salmonella survival. A six-strain cocktail of Salmonella serovars (Agona, Hadar, Heidelberg, Montevideo, Oranienburg, and Typhimurium) was added to yield 5 log cfu/g directly to about 5 kg of the two soils and moisture adjusted to 60 or 80% of field capacity (FC). Similarly, the Salmonella cocktail was mixed with fresh manure slurry from a hog nursery barn and the latter added to the two soils at 25 g/kg to achieve 5 log cfu/g Salmonella. Manure was mixed either throughout the soil or with the top kilogram of soil and the entire soil volume was adjusted to 60 or 80% FC. Soil treatments were stored 180 d at temperature sequences representing winter to summer (-18, 4, 10, 25 degrees C), spring to summer (4, 10, 25, 30 degrees C), or summer to winter (25, 10, 4, -18 degrees C) seasonal periods with each temperature step lasting 45 d. Samples for Salmonella recovery by direct plating or enrichment were taken at 0, 7, and 15 d post-inoculation and thereafter at 15-d intervals to 180 d. Salmonella numbers decreased during application to soil and the largest decreases occurred within the first week. Higher soil moisture, manure addition, and storage in the clay soil increased Salmonella survival. Salmonella survived longest (> or = 180 d) in both soils during summer-winter exposure but was not isolated after 160 d from loamy sand soil exposed to other seasonal treatments. For all but one treatment decimal reduction time (DRT45d) values calculated from the first 45 d after application were < or = 30 d and suggested that a 30-d delay between field application of manure in the spring or fall and use of the land would provide reasonable assurance that crop and animal contamination by Salmonella would be minimized.

Animals↗

Evaluation of zeolite for control of odorants emissions from simulated poultry manure storage.

Poultry operations are associated with emissions of aerial ammonia (NH3), volatile organic compounds (VOCs), and odor, and the magnitude of emissions is influenced by manure management practices. As a manure treatment additive, zeolites have been shown to have the potential to control NH3. Because of their properties it is also expected that zeolites could effectively adsorb VOCs and odor. The effectiveness of zeolite in controlling odor and VOCs was qualitatively evaluated in this controlled laboratory study involving simulated poultry manure storage. In the first two trials, zeolite was topically applied on nearly fresh laying hen manure at the rates of 0, 2.5, 5, and 10% (by weight). In the third trial, zeolite was topically applied at 5% with each addition of fresh manure into the storage vessel. Headspace samples from the emission vessels were collected with solid phase microextraction (SPME) and analyzed on a multidimensional-gas chromatograph-mass spectrometry-olfactometry (MDGC-MS-O) system for identification and prioritization of poultry manure odorants. Acetic acid, butanoic acid, isovaleric acid, indole, and skatole were consistently controlled in the headspace, with the reduction rate being proportional to the zeolite application rate. Dimethyl trisulfide and phenol were consistently generated, and with a few exceptions, the rate of generation was proportional to the application rate. Average reduction of the odor caused by all odorants evaluated with SPME-GC-O was 67% (+/-12%) and 51% (+/-26%) for the two topical applications, respectively, while no significant reduction of VOCs and odor was detected for the layered application.

Animals↗

Thermal pretreatment of the solid fraction of manure: impact on the biogas reactor performance and microbial community.

Application of thermal treatment at 100-140 degrees C as a pretreatment method prior to anaerobic digestion of a mixture of cattle and swine manure was investigated. In a batch test, biogasification of manure with thermally pretreated solid fraction proceeded faster and resulted in the increase of methane yield. The performances of two thermophilic continuously stirred tank reactors (CSTR) treating manure with solid fraction pretreated for 40 minutes at 140 degrees C and non-treated manure were compared. The digester fed with the thermally pretreated manure had a higher methane productivity and an improved removal of the volatile solids (VS). The properties of microbial communities of both reactors were analysed. The specific methanogenic activity (SMA) test showed that both biomasses had significant activity towards hydrogen and formate, while the activity with the VFA - acetate, propionate and butyrate - was low. The kinetic parameters of the VFA conversion revealed a reduced affinity of the microbial community from the CSTR fed with thermally pre-treated manure for acetate, propionate and butyrate. The bacterial and archaeal populations identified by t-RLFP analysis of 16S rRNA genes were found to be identical in both systems. However, a change in the abundance of the species present was detected.

Animals↗

Effect of scraping frequency in a freestall barn on volatile nitrogen loss from dairy manure.

The objective of this investigation was to evaluate the effect of scraping frequency (2x vs. 6x daily) on N volatilization from manure on the floor of a dairy free-stall barn. Three trials (crossover design) were conducted in the summers of 2001 and 2002, and in the winter of 2003. Nitrogen volatilization was estimated from the change in the N:P ratio in excreta at the time of excretion to the time when manure was scraped from the barn. Total N loss was considered a maximum estimate of NH3-N loss, because small amounts of nonammonia N may be volatilized. Nitrogen was determined after manure subsamples were lyophilized; P content was measured by direct current emission spectroscopy of ashed subsamples of manure. Lactating dairy cows were fed high-protein (18.5 to 19.3% crude protein), alfalfa-based diets. Average milk yield was 31.9 (SD = 7.4) kg/d. Scraping frequency had no effect on N loss in summer 2001. An average of 41% of excreted N, or 238 +/- 19.0 g of N/d per cow, was volatilized. For the trial in summer 2002, nitrogen volatilization was reduced from 50% of the excreted N with 2x to 46.7% with 6x, equivalent to 265 and 248 g of N lost/d per cow, respectively. Scraping had no effect on N volatilization during the winter trial. An average of 17.7% of excreted N was volatilized during the winter, equivalent to 109 +/- 11.0 g of N lost/d per cow. Scraping frequency of manure had little or no effect on N loss from manure in a freestall barn. Nitrogen loss during the winter was less than half of the loss during the summer.

Air Pollutants↗

Bacterial counts associated with sawdust and recycled manure bedding treated with commercial conditioners.

Bacteria counts associated with untreated organic bedding materials were compared with those of bedding treated with either an alkaline commercial bedding conditioner, acidic commercial bedding conditioner, or hydrated lime. Bedding materials were recycled manure and kiln-dried sawdust. The effects of bedding treatments on bacteria counts differed between bedding types. Each of the bedding treatments significantly reduced bacteria in recycled manure prior to use. The alkaline conditioner and hydrated lime effectively inhibited bacteria in recycled manure for 1 d. Bedding counts and teat swabs of cows housed on recycled manure treated with the alkaline conditioner were reduced on d 2. The use of the acid conditioner in recycled manure had little effect on bacteria in bedding. Sawdust differed from recycled manure in that bacteria in untreated sawdust prior to use were minimal, and populations increased rapidly during the first 2 d after use as bedding. The acid conditioner had a bacteriostatic effect in sawdust, evident by the reduction of bacteria on d 2. The alkaline conditioner and hydrated lime did not alter bacteria counts in sawdust compared with untreated sawdust. Antibacterial activity of each conditioner deteriorated between d 2 and d 6 in both beddings. The antibacterial activities of conditioners were related to the pH of bedding materials. The use of commercial bedding conditioners initially reduced bacterial counts; however, the antibacterial effects had diminished between d 2 and 6 after use in bedding.

Animals↗

Persistence of Escherichia coli and Salmonella in surface soil following application of liquid hog manure for production of pickling cucumbers.

Liquid hog manure is routinely applied to farm land as a crop fertilizer. However, this practice raises food safety concerns, especially when manure is used on fruit and vegetable crops. The objectives of this project were to evaluate the persistence of Escherichia coli and Salmonella in surface soil after application of liquid hog manure to fields where pickling cucumbers were grown and to verify the microbiological quality of harvested cucumbers. Mineral fertilizers were replaced by liquid hog manure at various ratios in the production of pickling cucumbers in a 3-year field study. The experimental design was a randomized complete block comprising four replicates in sandy loam (years 1, 2, and 3) and loamy sand (year 3). Soil samples were taken at a depth of 20 cm every 2 weeks after June application of organic and inorganic fertilizers. Vegetable samples were also taken at harvest time. Liquid hog manure, soil, and vegetable (washed and unwashed) samples were analyzed for the presence of Salmonella and E. coli. An exponential decrease of E. coli populations was observed in surface soil after the application of manure. The estimated average time required to reach undetectable concentrations of E. coli in sandy loam varied from 56 to 70 days, whereas the absence of E. coli was estimated at 77 days in loamy sand. The maximal Salmonella persistence in soil was 54 days. E. coli and Salmonella were not detected in any vegetable samples.

Animals↗

Phosphorus transport in overland flow in response to position of manure application.

Phosphorus (P) loss in overland flow varies with spatial distribution of soil P, management, and hydrological pathways. The effect of flow time, flowpath length, and manure position on P loss in overland flow from two central Pennsylvania soils packed in boxes of varying length (0.5, 1.0, 1.5, 2.75, and 4.0 m long x 15 cm wide x 5 cm deep) were examined by collecting flow samples at 5-min intervals for 30 min (50 mm h(-1) rainfall) without and with 75 kg P ha(-1) applied as swine (Sus scrofa) manure over 0.5 m of the box slope length at distances of 0 to 3.5 m from the downslope collection point. Dissolved reactive P concentration was more closely related to the proportion of clay in sediment of overland flow before (r = 0.98) than after (r = 0.56) manure application. This was attributed to the transport of larger, low-density particles after applying manure. The concentration of dissolved and particulate P fractions decreased with increasing flowpath length, due to dilution rather than sorption of P by surface soil during overland flow. Total P loss (mainly as particulate P) from the Watson channery silt loam (fine-loamy, mixed, active, mesic Typic Fragiudult) was more than from Berks channery silt loam (loamy-skeletal, mixed, active, mesic Typic Dystrudept), even with manure applied. Thus, while P loss in overland flow is affected by where manure is applied relative to flowpath length, initial soil P concentration should not be discounted when looking at areas of potential P loss within a watershed.

Environmental Monitoring↗

Anaerobic digestion of olive oil mill effluents together with swine manure in UASB reactors.

Combined anaerobic digestion of olive oil mill effluent (OME) with swine manure, was investigated. In batch experiments was shown that for anaerobic degradation of OME alone nitrogen addition was needed. A COD:N ratio in the range of 65:1 to 126:1 was necessary for the optimal degradation process. Furthermore, it was found that methane productions rates during digestion of either swine manure alone or OME alone were much lower than the rates achieved when OME and manure were digested together. Admixing OME with manure at a concentration of 5 to 10% OME resulted in the highest methane production rates. Using upflow anaerobic sludge blanket (UASB) reactors, it was shown that codigestion of OME with swine manure (up to 50% OME) was successful with a COD reduction up to 75%. The process was adapted for degradation of OME with stepwise increase of the OME load to the UASB reactor. The results showed that the high content of ammonia in swine manure, together with content of other nutrients, make it possible to degrade OME without addition of external alkalinity and without addition of external nitrogen source. Anaerobic treatment of OME in UASB reactors resulted in reduction of simple phenolic compounds such as mequinol, phenyl ethyl alcohol and ethyl methyl phenol. After anaerobic treatment the concentration of these compounds was reduced between 75 and 100%. However, the concentration of some degradation products such as methyl phenol and ethyl phenol were detected in significantly higher concentrations after treatment, indicating that the process has to be further optimised to achieve satisfactory removal of all xenobiotic compounds.

Agriculture↗

[Integrated effect of incorporation with different organic manures on CH4 and N2O emissions from rice paddy].

Field experiment was conducted in 2001 rice growing season to understand the effect of organic manure application on CH4 and N2O emissions. Five treatments of fertilizer including urea and NH4HCO3 used as chemical fertilizer (CF), chemical fertilizer + pig manure (CF + PM), chemical fertilizer + cow manure (CF + CM), chemical fertilizer + rapeseed cake (CF + RC) and chemical fertilizer + wheat straw (CF + WS) were dedicated to this experiment. Results from the field observations indicated that application of the organic manure did not change the seasonal pattern of CH4 and N2O emissions but significantly affected the seasonal amounts. Seasonal amount of CH4 emission from the treatments of CF + CM, CF + RC and CF + WS was respectively 45%, 252% and 250% higher than that from the treatment of CF, but the application of pig manure (CF + PM) did not significantly influence the CH4 emission. The N2O emissions, on the other hand, were reduced by 18%, 21% and 18% for the treatments of CF + PM, CF + CM and CF + WS, respectively. Incorporation of rapeseed cake enhanced the N2O emission, yielding an increment of 22%. On a time scale of 20-year period, the integrated global warming potential (GWP) of CH4 + N2O released during the rice growing season for the treatments of CF + RC, CF + WS and CF + CM was respectively 154%, 136% and 17% higher than that for the CF treatment, while a reduction of 10% was obtained for the CF + PM treatment. The calculation of the GWP per unit rice grain yield suggested that the application of pig manure took the slight advantages not only in reducing the GWP but also in improving the grain yield.

Manure↗

[Change of farmyard manure application in Shaanxi Province].

The change of farmyard manure (FYM) application in Shaanxi Province since 1986 was analyzed. The proportion of farmyard manure application to total nutrient decreased gradually with years, and was only 46.9% in 2000, which was reduced by 23% compared to the figure in 1986. Soil fertility was gradually reduced, and potassium shortage appeared in farmlands of some regions. The farmyard manure were consumed mainly on grain crops in terms of overall amount of manure inputs, bu application rates of manure to fruit trees and vegetables were higher than that of grain crops in terms of per unit area. Consumption of manure obviously increased in apple since middle of 1990's. The reason of the FYM consumption decrease was also analyzed, and some suggestions were made.

China↗

Effect of manure application timing, crop, and soil type on phosphorus leaching.

Phosphorus (P) leaching losses from manure applications may be of concern when artificial drainage systems allow for hydrologic short-cuts to surface waters. This study quantified P leaching losses from liquid manure applications on two soil textural extremes, a clay loam and loamy sand soil, as affected by cropping system and timing of application. For each soil type, manure was applied at an annual rate of 93 800 L ha(-1) on replicated drained plots under maize (Zea mays L.) in early fall, late fall, early spring, and as a split application in early and late spring. Manure was applied on orchardgrass (Dactylis glomerata L.) in split applications in early fall and late spring, and early and late spring. Drain water was sampled at least weekly when lines were flowing, and outflow rate and total P content were determined. High P leaching losses were measured in the clay loam as soon as drain lines initiated flow after manure application. Flow-weighted mean P leaching losses on clay loam plots averaged 39 times higher (0.504 mg L(-1)) than those on loamy sand plots (0.013 mg L(-1)), and were above the USEPA level of concern of 0.1 mg L(-1). Phosphorus losses varied among application seasons on the clay loam soil, with highest losses generally measured for early fall applications. Phosphorus leaching patterns in clay loam showed short-term spikes and high losses were associated with high drain outflow rates, suggesting preferential flow as the main transport mechanism. Phosphorus leaching from manure applications on loamy sand soils does not pose environmental concerns as long as soil P levels remain below the saturation level.

Agriculture↗

[Bioleaching of fly ash from municipal solid waste incinerator using sewage sludge and pig manure as culture media].

A mixed culture of Acidihiobacillus ferrooaidans and Acidihiobacillus thiooxidans was used to leach heavy metals from municipal solid waste incineration fly ash (MSWI fly ash). This study explored the possibility of using sewage sludge or pig manure as nutrients for supporting the growth of the leaching bacteria and allowing metal solubilization like a synthetic mineral medium. In contrast to pig manure, there is a high ability for acidification of the fly ash and solubilization of toxic metals using sewage sludge at the same content. After 15 d of bioleaching, the following removal efficiencies were obtained for the treatment with the addition of 1% sewage sludge: Cd 88.1%; Zn 78.7%; Cu 69.6%, whereas their removal efficiencies for the treatment with the addition of 1% pig manure were 82.4%, 73.5% and 60.0%, respectively. Results demonstrate that the inhibition by sewage sludge DOM is much more significant than by pig manure DOM at the same concentration level. The dissolved organic carbon in excess of 400 and 150 mg/L was inhibitory to the bacterial growth using sludge DOM and manure DOM, respectively. Compared with sewage sludge, pig manure contained a higher fraction of DOM with molecular size <1000, which led to its higher toxicity.

Acidithiobacillus↗

[Influence of straw manuring on the qualitative properties of the organic soil substances of a Lehm-Braunschwarzerde (author's transl)].

It is reported on the influence of a long-term field trial with straw manuring at different terms and different nitrogen fertilization steps on the qualitative properties of the organic soil substance. Straw manuring in autumn increased the carbon content in the soil. Increasing amounts of nitrogen intensified this effect. Straw manuring in spring only with simultaneously applied quantities of 100 and 200 kg N/ha, respectively, brought about an increased content of organic substances in soil. With straw manuring in autumn, fractionation of the organic substance in soil showed a smaller amount of humic acids. Increasing nitrogen addition reduced this amount still more than in the plots with straw manuring in spring. The quota of fulvic acids increased. Therefore, the higher content of organic substances, obtained with straw manuring in autumn, is based on an increase of the pre-stages of humic acids and non-humic matter.

Benzopyrans↗

Development of a hydrophilic interaction chromatography-mass spectrometry assay for spectinomycin and lincomycin in liquid hog manure supernatant and run-off from cropland.

A specific and sensitive analytical method was developed to extract and quantify spectinomycin and lincomycin in liquid hog manure supernatant and simulated rainfall run-off from manure-treated cropland. Sample extracts were prepared using solid-phase extraction (SPE) employing a weak cation-exchange resin (Oasis WCX) for extraction of spectinomycin. An Oasis HLB cartridge was used for extraction of lincomycin. Hydrophilic interaction chromatography (HILIC) was used to obtain the necessary separation of the two antibiotics from interfering compounds and to provide baseline separation. Analytes were detected using atmospheric pressure chemical ionization tandem mass spectrometry. Extraction recoveries were 77% for lincomycin and 84% for spectinomycin in liquid hog manure supernatant, and 89% for lincomycin and 95% for spectinomycin in run-off water. The corresponding limits of quantitation were 6.0 and 0.040 microg l(-1) for spectinomycin and lincomycin, respectively, in liquid hog manure supernatant and 0.2 and 0.008 microg l(-1) for spectinomycin and lincomycin, respectively, in run-off from manure treated cropland. The method is suitable for monitoring the environmental fate and transport of these two antibiotics in both liquid hog manure and agricultural field run-off.

Animals↗

Quantification of veterinary antibiotics (sulfonamides and trimethoprim) in animal manure by liquid chromatography-mass spectrometry.

A fast and cost effective method was developed to extract and quantify residues of veterinary antimicrobial agents (antibiotics) in animal manure by liquid-liquid extraction and liquid chromatography-mass spectrometry. The compounds investigated include six sulfonamides, one metabolite, and trimethoprim. The method was performed without sample clean up. Recoveries from spiked manure slurry samples (spike level = 1 mg/kg) were as follows: sulfaguanidine (52%), sulfadiazine (47%), sulfathiazole (64%), sulfamethazine (89%), its metabolite N4-acetyl-sulfamethazine (88%), sulfamethoxazole (84%), sulfadimethoxine (51%), and trimethoprim (64%). Relative standard deviations of the recoveries were less than 5% within the same day and less than 20% between days. The limit of quantification was below 0.1 mg/kg liquid manure slurry for all compounds and calibration curves obtained from extracts of spiked samples were linear up to a level of 5 mg/kg liquid manure, except for trimethoprim (0.01-0.5 mg/kg). Analysis of six grab samples taken in Switzerland from manure pits on farms where medicinal feed had been applied revealed total sulfonamide concentrations of up to 20 mg/kg liquid manure.

Animals↗

The effects of application of poultry manure to crude oil polluted soils on maize (Zea mays) growth and soil properties.

An investigation of the effects of application of poultry manure to crude oil polluted soils on the growth of maize and soil properties was carried out under natural conditions at the Ambrose Alli University, Ekpoma, Nigeria. Potted maize plants were treated to crude oil pollution at four different levels (0, 25, 50 and 75 mL) and amended with poultry manure at four rates of application (0, 50, 100 and 150 kg ha(-1)) two weeks after pollution. Results obtained showed that percent survival rate, plant height and dry matter yield decreased with increase in crude oil contamination. For example % seed germination decreased from 93 to 0% as crude oil increased from 0 to 75 mL without poultry manure application while plant height decreased from 97 to 20 cm. However when amended with poultry manure, statistical analysis showed that the highest rate of crude oil application (75 mL) and the 150 kg ha(-1) rate poultry manure application affected maize growth, dry matter yield and soil properties significantly. For example at the 75 mL crude oil application, plant height increased from 20 to 149 cm as level of manure applied increased from 0 to 150 kg ha(-1) while dry matter yield increased from 27 to 58 g.

Animals↗

Phosphorus fractions in manure from growing pigs receiving diets containing micronized peas and supplemental enzymes.

Different livestock feeds manipulations have been reported to reduce the total P concentration in manure. Information on the influence of these dietary manipulation strategies on the forms of P in manure is, however, limited. This study was, therefore, conducted to investigate the effect of diet manipulation through feed micronization and enzyme supplementation on the forms of P in swine manure. Eight growing pigs were fed four diets: barley-raw pea (BRP), barley-micronized pea (BMP), barley-raw pea with enzyme (BRPE), and barley-micronized pea with enzyme (BMPE) in a 4 x 4 Latin square design. Because we are interested in the effect of enzyme cocktail and pea micronization on manure P, we did not reduce the non-phytate P with enzyme addition in this study. The fecal material and urine were collected and analyzed for total P. Fecal material was fractionated to determine the total P in H2O-, NaHCO3-, NaOH-, and HCl-extractable fractions. The total P in the residual fractions was also determined. About 98% of the total P excreted by the pigs was found in the fecal material. Inclusion of micronized pea in pig diet did not have any significant effect (p > 0.1) on either the total P or the different P fractions in the manure. The labile P (the sum of H2O-P and NaHCO3-P) was significantly reduced (p < 0.05) by the addition of enzyme to swine diets. Pigs fed the BRPE and BMPE had 14 and 18% lower labile P, respectively, compared with pigs fed the BRP. Enzyme addition to pig diets reduced not only the total P in manure, but also the labile P fraction, which is of great environmental concern. Thus, the potential of P loss to runoff and the subsequent eutrophication can be reduced by enzyme addition to pig diets.

Animal Feed↗