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At least 235 records · Page 13Linked to original sources

Myelopoiesis in lymph nodes of mice. Enhancement effect by the injection of trypan blue or India ink after birth.

Myelopoiesis in lymph nodes was studied in newborn mice under normal conditions and after treatment with trypan blue and carbon particles. In the mouse lymph node, myelopoiesis normally takes place between the fourth and twelfth day after birth, then decreases while an intense proliferation of the lymphocytic population simultaneously takes place. Myelopoiesis manifests itself as clusters of cells, mainly of the neutrophilic line, located around post-capillary venules (PCV's) in the medulla. The injection of trypan blue or carbon particles (India ink) causes an enhancement of myelopoiesis and a delay in the development of the lymphocytic population. Myelopoiesis includes not only granulopoiesis but also the formation of erythroid clusters and occasional megakaryocytes. Three possibilities should be considered to explain the enhancement of myelopoiesis: 1) blockade of the reticuloendothelial system caused by overloading with particulate matter; 2) induction of humoral myelopoiesis-stimulating factors; and 3) stimulation of pluripotent stem cells through dye injection.

Animals↗

A warning device which indicates the stoppage of paper in an ink-writing recorder.

A simple and inexpensive warning device is described which indicates when the paper has stopped moving beneath the pens of an inkwriting polygraph. The device operates on the principle that the electrical resistance of the paper under the recorder pen is markedly reduced when the paper stops moving and is made wetter by the ink. A buzzer sounds within a few seconds of the paper stopping and so reduces the possibility of failing to record otherwise irretrievable information, for example during all-night recordings of the EEG in sleep research.

Electronics, Medical↗

The india ink injection technique to assess the depth of experimental burn wounds.

A detailed description of the India ink injection technique for assessment of experimental burn wound depth is presented with emphasis upon special technical manipulations. Assessment of the burn depth is mandatory for the establishment of a burn wound model as well as to reproduce consistent data among different investigators.

Animals↗

Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase.

This paper describes the optimisation of a screen-printing water-based carbon ink containing cobalt phthalocyanine (CoPC) and glucose oxidase (GOD) for the fabrication of a glucose biosensor. To optimise the performance of the biosensor, the loadings of the electrocatalyst (CoPC) and enzyme (GOD) were varied. It was found that the maximum linear range was achieved with a CoPC loading of 20% (m/m, relative to the mass of carbon) and a GOD loading of 628 U per gram of carbon. In our studies we chose to employ chronoamperometry, as this technique is commonly used for commercial devices. The optimum operating applied potential was found to be +0.5 V, following an incubation period of 60 s. The optimum supporting electrolyte was found to be 0.05 M phosphate buffer at pH 8.0, which resulted in a linear range of 0.2-5 mM, the former represents the detection limit. The sensitivity was 1.12 microA mM(-1). The effect of temperature was also investigated, and it was found that 40 degrees C gave optimal performance. The resulting amperometric biosensors were evaluated by measuring the glucose concentrations for 10 different human plasma samples containing endogenous glucose and also added glucose. The same samples were analysed by a standard spectrophotometric method, and the results obtained by the two different methods were compared. A good correlation coefficient (R(2) = 0.95) and slope (0.98) were calculated from the experimental data, indicating that the new devices hold promise for biomedical studies.

Biosensing Techniques↗

Concentrated hydroxyapatite inks for direct-write assembly of 3-D periodic scaffolds.

Hydroxyapatite (HA) scaffolds with a 3-D periodic architecture and multiscale porosity have been fabricated by direct-write assembly. Concentrated HA inks with tailored viscoelastic properties were developed to enable the construction of complex 3-D architectures comprised of self-supporting cylindrical rods in a layer-by-layer patterning sequence. By controlling their lattice constant and sintering conditions, 3-D periodic HA scaffolds were produced with a bimodal pore size distribution. Mercury intrusion porosimetry (MIP) was used to determine the characteristic pore size and volume associated with the interconnected pore channels between HA rods and the finer pores within the partially sintered HA rods.

Biocompatible Materials↗

Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge.

Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 degrees C, both isolates revealed that N2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 degrees C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source.

Azotobacter↗

Synthesis of myo-inositol 1,2,3-tris- and 1,2,3,5-tetrakis(dihydrogen phosphate)s as a tool for the inhibition of iron-gall-ink corrosion.

Two myo-inositol phosphates, myo-inositol 1,2,3-tris(dihydrogen phosphate) and myo-inositol 1,2,3,5-tetrakis(dihydrogen phosphate), have been synthesised in several steps from myo-inositol (in Chem. Abstr.: d-myo-inositol) in the form of their sodium salts. They were shown to prevent iron-gall-ink decay in cellulose items at the same level as phytic acid dodecasodium salt.

Corrosion↗

Enzymatic versus chemical deinking of non-impact ink printed paper.

Enzymatic versus chemical deinking is examined for MOW and photocopy prints. Several enzymatic preparations and two fibre/ink particle separation methods are tested. Deinking was monitored by image analysis and standard pulp and paper characterisation procedures. The effectiveness of the fibre/ink particle separation method depends on the ink particle's size: for smaller particles a washing step is recommended whereas for larger particles, the use of flotation is necessary. The enzymatic treatment is a competitive alternative for MOW and photocopy paper deinking. However, the process requires the selection of an adequate enzymatic preparation for each paper grade.

Biodegradation, Environmental↗

Ink-jet printing for micropattern generation of laminin for neuronal adhesion.

In order to achieve defined adhesion and neurite outgrowth, the growth substrate must be patterned in an appropriate way. We utilised ink-jet printing by means of a piezo-based microdispenser to create defined line patterns of a polymer with typical dimensions of 100 microm width on glass, silicon, gold and carbon substrates. Vinnapas, a co-polymer of vinyl acetate and ethylene, was mixed with the extracellular matrix protein laminin to achieve neuronal adhesion on the surface of the patterns. It could be demonstrated that the laminin entrapped in the polymer lines can be recognised by a specific antibody. Adhesion of embryonic chicken forebrain neurones is following the prepared lines, and identity of adhering cells could be shown by neurofilament staining. These findings open the route for the generation of complex small neuronal arrays and for the electrochemical investigation of the obtained neuronal matrix.

Animals↗

The role of zeolites in wastewater treatment of printing inks.

The adsorption of residual organic pollutants from flocculated printing ink wastewater onto several synthetic zeolites was investigated as a finishing method for additional reduction of TOC. The nonselective removal of total organic content was studied. The amount of adsorbed organics was largest for ZSM-5 and NH4-Beta while the other zeolites studied showed lower efficiency, suggesting that adsorption is independent of pore structure. The adsorption rates of organic pollutants were fast. Although the TOC removal increases with increasing amount of zeolite, because of the necessity of additional filtration to lower turbidity to required levels, 5.0 g/L of zeolite was found to be optimum. The 88% reduction of TOC obtained with a single flocculation treatment was improved with the combination of flocculation and adsorption with ZSM-5 which resulted in the overall TOC efficiency of 95%. The addition of zeolites in decantated supernatant water, obtained after flocculation, was also studied in order to assess the effect of floc on zeolite capacity. A decrease in adsorption capacity occurred only if a coagulant concentration less than optimal was applied. Removal efficiency then decreased by around 10%. It was concluded that flocculation followed by adsorption with zeolites is an effective treatment method for this kind of wastewater.

Adsorption↗

Monitoring and characterization of ink vehicle autoxidation by inverse gas chromatography.

It is now well-established that increased usage of vegetable oils in offset ink formulas aggravates the deinking problems in recycling plants during summer months. The seasonal loss of brightness of recycled paper has been ascribed to increased bonding of oxidatively aged prints to the paper surface. The progress of the oxidative aging of soya bean and linseed oils was followed by inverse gas chromatography. We first report here the rate and extent of vegetable oil oxidation, by measuring the changes in Kovats retention index as a function of the oxidation time. We then characterized the physicochemical changes accompanying the oxidation of vegetable oils from measurements of the partial heat of mixing in the infinite dilution regime.

Chromatography, Gas↗

Ink used as first aid treatment of a scald.

We report here a case of a childhood scald injury where immediate first aid consisted of the application of fountain pen ink. Continuing education is required to inform parents of the use of cold water in the immediate first aid treatment of scalds.

Burns↗

Dynamics of water-soluble carbon substances and microbial populations during the composting of de-inking paper sludge.

Composting is an alternative method to dispose of de-inking paper sludge (DPS). Today, few studies have investigated the water-soluble carbon (WSC) substances as indicators of the decomposition process and the microbial changes taking place during the composting of DPS. Accordingly, the goal is to study their dynamics during the composting of DPS at three nitrogen levels, 0.6%, 0.7% or 0.9% total N, using mechanical turning. The changes in WSC substances, microbial biomass carbon (MBC) and, total and DPS microbial populations were monitored during 24 weeks. Also, microorganisms were identified and tested for the production of selected enzymes. Regardless of N treatments, the dynamic of WSC substances indicated that cellulose and hemicellulose fractions of DPS fibers were mainly biodegraded during the first 8 weeks while the more resistant carbon (C) fractions were biodegraded thereafter. MBC also evolved regardless of N treatments but was correlated to WSC substances. Its high values decline mostly after 12 weeks indicating the exhaustion of this source of C energy for microbial growth and the stabilisation of DPS organic matter. The dynamic and identified microorganisms were comparable to those observed in other composting processes. However, the results pointed out that those mostly implicated in the hydrolysis of DPS fibers were the thermophilic actinomycetes and fungi and, by comparison to the 0.6% or 0.7% N treatment, they decreased in presence of the 0.9% N treatment. Most microorganisms were hemicellulolytic bacteria, while actinomycetes and fungi were capable of degrading a wide variety of substrates. Overall, dynamics of WSC substances and microbial populations indicated that during composting, DPS decomposition obey a two phase decay while, contrary to the lowest N treatment, the 0.9% N treatment has slowed down this process by harming the important microbial populations implicated in the degradation of DPS fibers.

Actinobacteria↗

Edge transfer lithography using alkanethiol inks.

Edge lithographic patterning techniques are based on the utilization of the edges of micrometer-sized template features for the reproduction of submicrometer structures. Edge transfer lithography (ETL) permits local surface modification in a single step by depositing self-assembled monolayers onto a metal substrate selectively along the feature edges of an elastomeric stamp. In this report two stamp designs are described that now allow for the use of alkanethiol inks in ETL and their use as etch resists to reproduce submicrometer structures in gold. Anisotropically modified stamps are shown to combine the potential for very high-resolution patterning with the versatility and simplicity of microcontact printing.

Alkanes↗

India ink pinprick assessment of age-related changes in the cricoarytenoid joint (CAJ) articular surfaces.

Age-related changes in the articular surfaces of the cricoarytenoid joints (CAJ) of 12 human Caucasian male larynges (6 age 19 to 30 years; 6 age 50 to 80 years) were investigated. Differences in color, roughness, ossification, and surface fiber organization were studied. These were correlated with changes in collagen fiber arrangement on the articular surfaces determined by an India ink pinprick technique. Consistent patterns of orientation of collagen fibers in CAJ surfaces were identified in young and old groups. Older articular surfaces exhibited extensive fibrillation and ossification, suggesting that articular cartilage undergoes alteration in ground substance and/or fiber structure as a function of age. CAJ cartilage changes in older males may limit range of motion of arytenoid cartilages and reduce degree and extent of vocal fold closure. These structural changes may produce negative functional consequences during voice production such as diminished vocal quality and reduced vocal intensity due to air leakage through incompletely or loosely approximated vocal folds.

Adult↗

Phase of molecular ink in nanoscale direct deposition processes.

We report the first observation of a phase transition in a nanoscale direct deposition process. This transition involves the melting of molecular ink layers in dip-pen nanolithography, and it is observed by measuring the temperature dependence of the growth rate of the deposited pattern. The results are interpreted using a diffusion equation approach in conjunction with a "double-molecular-layer" model of the adsorbed molecules on the atomic force microscope tip. The theory provides a qualitative explanation for the dependence of the pattern growth rate on solvent and adsorbed water as well as on temperature.

Adsorption↗

Electrophoretic ink using urea-formaldehyde microspheres.

A kind of electronic ink, which is supported on indium-tin-oxide (ITO) glass before polymerization, is prepared using urea and formaldehyde resin as wall materials, in which TiO(2) modified with PMMA are dispersed in tetrachloroethylene (TCE) using a mixture of oil blue dyes and charge control additive (span80). Rotary viscometer, Fourier transform infrared spectroscopy (FT-IR) and optical microscopy are used to characterize the particles, respectively. The electrophoretic mobility of the microcapsules to electric field is also investigated with two parallel electrodes.

Capsules↗

Intercostal nerve block: spread of india ink injected to the rib's costal groove.

Three and five millilitre of india ink were injected bilaterally into the costal groove of the 9th or 10th ribs of 14 cadavers. The spread was observed and the costal groove of the rib injected was dissected, with the one above and one below. Nunn and Slavin's study (1980) of similar injections in two cadavers indicated that an injection of one costal groove blocked not only the intercostal nerve of that groove, but at least the one above and below it. The present study verified the author's previous report that only the intercostal nerve in the costal groove of the rib injected is anaesthetized.

Humans↗