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The tempering of medical anthropology: troubling natural categories.

This article reviews an approach in medical anthropology that commenced in the early 1980s and that continues to the present day in which biomedical knowledge and practices are systematically incorporated into anthropological analyses. Discussion then focuses on contributions made by feminists and medical anthropologists to the literature on medicalization and resistance, illustrating how the ethnographic approach has been crucial in critically reconceptualizing and situating these concepts historically and cross-culturally. The concept of local biologies is introduced in the third section of the article in creating the argument that the coproduction of biologies and cultures contributes to embodied experience, which, in turn, shapes discourse about the body. Subjective reporting at menopause provides an illustrative case study of local biologies in action. The final part of the article takes up the question of the moral economy of scientific knowledge. Comparative ethnographic work in intensive care units in Japan and North America reveals how a moral economy is put into practice in connection with brain-dead bodies and the procurement of organs from them. Medical anthropological contributions to policy making about biomedical technologies is briefly considered in closing.

Anthropology, Cultural↗

Ruminant nutrition from an environmental perspective: factors affecting whole-farm nutrient balance.

Nutrient budgeting strategies focus primarily on recycling manure to land as fertilizer for crop production. Critical elements for determining environmental balance and accountability require knowledge of nutrients excreted, potential nutrient removal by plants, acceptable losses of nutrients within the manure management and crop production systems, and alternatives that permit export of nutrients off-farm, if necessary. Nutrient excretions are closely related to nutrient intake and can be predicted by subtracting predicted nutrients in food animal products exported from the farm from total nutrients consumed. Intensifying crop production with double- or triple-cropping often is necessary for high-density food animal production units to use manure without being forced to export manure or fertilizer coproducts to other farms. Most manures are P-rich relative to N largely because of 1) relatively large losses of volatilized NH3, most of it converted from urea in urine, 2) denitrification losses in soil under wet, anaerobic conditions, and 3) ability of many crops to luxury-consume much more N than P. Most soils bind P effectively and P usually is permitted to accumulate, allowing for budgets to be based on N. However, P budgeting may be required in regions where surface runoff of P contributes to algae growth and eutrophication of surface waters or where soil P increases to levels of concern. Research is needed to determine whether dietary P allowances can be lowered without detriment to animal production or health in order to lower P intake and improve N:P ratios in manure relative to fertilization needs.

Animal Nutritional Physiological Phenomena↗

Efficacy of sucrose and milk chocolate product or dried porcine solubles to increase feed intake and improve performance of lactating sows.

Two experiments were conducted to determine the voluntary feed intake and performance of lactating sows fed diets containing a sucrose/milk chocolate product (MCP) blend (Exp. 1) or dried porcine solubles (DPS; Exp. 2). Dried porcine solubles is a coproduct of heparin extraction from porcine small intestines. In Exp. 1, mixed-parity sows (n = 108) at two research centers were assigned to a corn-soybean-meal-based diet formulated to contain 0.9% total lysine or a similar diet that contained 4% sucrose and 2% MCP on an as-fed basis. Sows were allowed ad libitum access to dietary treatments from the day of farrowing until pigs were weaned at approximately 21 d postpartum. Diet had no significant effect on voluntary feed intake of sows during lactation, backfat depth, or postweaning interval to estrus, but it had variable effects on body weight changes. Inclusion of the sucrose/MCP blend in diets elicited a 2% improvement in litter weaning weight at one research center and a 6% depression in litter weaning weight at the other center (diet x research center, P < 0.05). Litter size throughout lactation was unaffected by dietary treatment. In Exp. 2, mixed-parity sows (n = 119) at two research centers were assigned to corn-soybean meal-based diets formulated to contain 0.9% total lysine with 0, 1.5, or 3.0% added DPS. Sows were assigned to dietary treatments within research center, farrowing group, and parity at parturition. Dried porcine solubles tended to increase (P < 0.10) total feed consumed in the first 9 d of lactation and average daily feed intake over the entire lactation (6.03, 6.53, and 6.30 kg) for sows fed 0, 1.5, and 3.0% DPS, respectively. Litter size and weight on d 18 of lactation were not affected by concentration of DPS in the diet. Days from weaning to estrus and percentage of sows displaying estrus were not influenced by diet. We conclude that inclusion of the sucrose/MCP blend in the diet for lactating sows had no consistent effect on voluntary feed intake of sows and weight gain of nursing pigs. Inclusion of DPS at 1.5 or 3.0% tended to improve feed intake of lactating sows but had no significant influence on litter performance.

Animal Feed↗

Slow-release urea and highly fermentable sugars in diets fed to lactating dairy cows.

This experiment was designed to test the inclusion of highly fermentable sugars (FS) in dairy rations and their interactions with a slow-release urea (SU) product. The FS are a blend of liquid coproducts from the corn milling and cheese industries, and the SU is calcium chloride urea. Eight multiparous and 4 primiparous Brown Swiss cows (117 +/- 46 d in milk) were blocked by parity and utilized in a multiple Latin square design. Basal diets were formulated for 16.6% crude protein and 1.55 Mcal/kg of net energy for lactation and contained 35% of dietary dry matter as corn silage, 15% alfalfa hay, 34% of a concentrate mix containing varying proportions of ground shelled corn and soybean meal, and 16% of a constant concentrate premix. The premix consisted of equal proportions of corn distillers grains, soybean hulls, expeller soybean meal, vitamins, and minerals across all diets. Diets contained either no supplemental FS (NFS) or FS (8.64% RationMate) and either no SU (NSU) or SU (0.61% Ruma Pro) in a 2 x 2 factorial arrangement of treatments. Feeding FS tended to decrease milk production compared with feeding NFS. Milk fat percentage was increased for cows fed FS compared with NFS. Feeding SU decreased dry matter intake and increased feed efficiency compared with cows fed NSU. Dietary treatment had no effect on energy-corrected milk, milk fat yield, milk protein percentage, or milk urea N. Feeding FS increased the molar proportion of ruminal butyrate and decreased the molar proportion of propionate; however, no other effects were observed on ruminal fermentation. No interactions between FS and SU were observed. It was concluded that the replacement of corn and soybean meal with dietary FS increased milk fat percentage and that the replacement of soybean meal with SU significantly improved feed efficiency.

Animal Feed↗

Microbial lipopeptides induce the production of IL-17 in Th cells.

Naive Th cells can be directed in vitro to develop into Th1 or Th2 cells by IL-12 or IL-4, respectively. In vivo, chronic immune reactions lead to polarized Th cytokine patterns. We found earlier that Borrelia burgdorferi, the spirochaete that causes Lyme disease, induces Th1 development in alpha beta TCR-transgenic Th cells. Here, we used TCR-transgenic Th cells and oligonucleotide arrays to analyze the differences between Th1 cells induced by IL-12 vs those induced by B. burgdorferi. Transgenic Th cells primed with peptide in the presence of B. burgdorferi expressed several mRNAs, including the mRNA encoding IL-17, at significantly higher levels than Th cells primed with peptide and IL-12. Cytometric single-cell analysis of Th cell cytokine production revealed that IL-17 cannot be categorized as either Th1 or Th2 cytokine. Instead, almost all IL-17-producing Th cells simultaneously produced TNF-alpha and most IL-17(+) Th cells also produced GM-CSF. This pattern was also observed in humans. Th cells from synovial fluid of patients with Lyme arthritis coexpressed IL-17 and TNF-alpha upon polyclonal stimulation. The induction of IL-17 production in Th cells is not restricted to B. burgdorferi. Priming of TCR-transgenic Th cells in the presence of mycobacterial lysates also induced IL-17/TNF-alpha coproduction. The physiological stimulus for IL-17 production was hitherto unknown. We show here for the first time that microbial stimuli induce the expression of IL-17 together with TNF-alpha in both murine and human T cells. Chronic IL-17 expression induced by microbes could be an important mediator of infection-induced immunopathology.

Animals↗

T1/ST2 expression is enhanced on CD4+ T cells from schistosome egg-induced granulomas: analysis of Th cell cytokine coexpression ex vivo.

Th cells are categorized into subsets based on the cytokine production of in vitro-differentiated Th populations. For in vivo-differentiated Th subsets, little is known about the heterogeneity of cytokine production in single cells. We recently described a molecule, T1/ST2, that is preferentially expressed on the surface of Th2 cells. Here we combined high-gradient magnetic cell separation with four-color single-cell cytometry to analyze simultaneously three intracellular cytokines and T1/ST2 surface expression on CD4+ cells from lungs containing granulomas induced by Schistosoma mansoni eggs. T1/ST2 was highly up-regulated on CD4+ T cells from hepatic granulomas and granulomatous lungs. T1/ST2+ cells from granulomatous lungs preferentially produced type 2 cytokines ex vivo. In the total CD4+ population, coexpression of type 1 and type 2 cytokines occurred frequently. However, such coproduction was drastically reduced in T1/ST2+ cells compared with T1/ST2- cells. Coexpression of type 1 and type 2 cytokines was also rare in cells simultaneously producing two cytokines of one type. These findings indicate that individual CD4+ T cells in vivo have different levels of commitment to a certain Th phenotype. Coexpression of two type 2 cytokines or production of one type 2 cytokine together with surface expression of T1/ST2 indicate advanced commitment to the Th2 phenotype.

Animals↗

[Transfection with the E1A and E1B-19kDa oncogenes does not prevent rat embryo fibroblasts from cell cycle arrest after gamma-radiation].

Introduction of the E1A early region of the human adenovirus type 5 impairs the ability of mammalian cells to arrest the cell cycle at G1/S after damage. Two-parameter fluorescent-activated cell sorting (FACS) with iododeoxyuridine revealed the radiation-induced G1/S arrest in rat embryo fibroblasts transformed with the complementing E1A + E1B-19 kDa oncogenes. This was due to selective inhibition of CycIE/Cdk2-associated kinase activity, while activities of type 2 kinase and of CyclA/Cdk2 complexes remained unchanged. The inhibitor of G1-phase cyclin kinases, p21/Waf1, was accumulated and interacted with target kinases both in normal and in transformed cells after irradiation. As shown by immunoprecipitation, p21/Waf1 formed complexes with the E1A on coproducts in the transformants, which possibly accounted for its functional inactivation. Kinase modification in cyclin-kinase complexes was assumed to play a key role in regulation of cyclin-dependent kinases in the transformants with inactivated p21/Waf1.

Adenovirus E1A Proteins↗

Association of human polyomavirus JCV with colon cancer: evidence for interaction of viral T-antigen and beta-catenin.

Infection of the gastrointestinal tract by the human polyomavirus, JCV, which has been frequently detected in raw urban sewage, can occur via intake of contaminated water and food. In light of earlier reports on the tumorigenecity of JCV, we investigated the presence of the JCV genome and the expression of viral proteins in a collection of 27 well-characterized epithelial malignant tumors of the large intestine. Results from gene amplification revealed the presence of the viral early genome in 22 of 27 samples. Expression of the viral oncogenic early protein, T-antigen, and the late auxiliary protein, Agnoprotein, was observed in >50% of the samples. The absence of the viral capsid protein in the tumor cells excludes productive replication of the virus in neoplastic cells. Laser capture microdissection confirmed the presence of the JCV genome and expression of T-antigen in precancerous villous adenomas and regions of invasive adenocarcinoma. The ability of JCV T-antigen to interact with beta-catenin and the nuclear detection of beta-catenin in T-antigen-positive cells suggests dysregulation of the Wnt pathway in the tumor cells. The coproduction of T-antigen and beta-catenin in colon cancer cells enhanced transcription of the c-myc promoter, the downstream target of beta-catenin. These observations provide evidence for a possible association of JCV with colon cancer and suggest a novel regulatory role for T-antigen in the deregulation of the Wnt signaling pathway through beta-catenin in tumors of the gastrointestinal tract.

Adenocarcinoma↗

Production of carbohydrases by Sclerotium rolfsii.

Coproduction of alpha-amylase, beta-amylase, amyloglucosidase, cellulase, xylanase, pectinase and beta-galactosidase by Sclerotium rolfsii was studied on various polysaccharides. Starch induced alpha-amylase, beta-amylase, amyloglucosidase and beta galactosidase; cellulose induced cellulase, xylanase, pectinase and beta-galactosidase; and pectin induced pectinase and beta-galactosidase. None of the enzymes studied except beta-galactosidase were induced on xylan. Group controlled mechanism for production of carbohydrases by Sclerotium rolfsii is suggested.

Enzyme Induction↗

Co-production dynamics and time dollar programs in community-based child welfare initiatives for hard-to-serve youth and families.

Hard-to-serve youth and families residing in high-poverty communities often have multiple, interlocking needs. These needs necessitate complex service models. The complex model described in this article combines a unique approach to wraparound services with a coproduction framework and related theories. The model aims to improve outcomes for vulnerable youth and their families, simultaneously strengthening communities by employing residents and engaging participants in community service. Examples derived from current pilot projects illustrate co-production's importance for other child welfare initiatives.

Child↗

A novel on-site system for the treatment of pharmaceutical laboratory wastewater by supercritical water oxidation.

For the on-site treatment of laboratory waste, we have been developing a compact-sized reaction system for the treatment of laboratory wastewater using supercritical water oxidation (SCWO) technology. Pharmaceutical laboratory wastewater is one of the most difficult wastewaters to treat because of its high concentration of halogenated organic compounds. We proposed a new cascade process in which two reactors are consecutively combined, carrying out hydrolysis in the first reactor followed by SCWO in the second reactor, for the complete removal of halogenated organic compounds. Dichloromethane was chosen as a representative model of chlorinated compounds. There have been many previous studies on the hydrolysis of dichloromethane, which results in the coproduction of formaldehyde and HCl. However, there has been less investigation on the kinetics of formaldehyde oxidation in supercritical water. In this study, we focus on the oxidation of formaldehyde in supercritical water with and without a catalyst. As a result, formaldehyde can be completely decomposed at 400 degrees C and 25 MPa within a very short contact time in a heterogeneous system with a MnO(2) catalyst.

Drug Compounding↗

A new murine CD4+ T cell subset with an unrestricted cytokine profile.

CD4+ T cell clones were derived from mice immunized to keyhole limpet hemocyanin to characterize the cytokine profiles of newly isolated clones. Surprisingly, several of the clones had an unrestricted profile, producing IL-2, IL-3, IL-4, IFN-gamma, and TNF after either Con A or Ag stimulation. The coproduction of IL-2 and IL-4 was confirmed at the mRNA level. Subclones were derived which contained RNA transcripts for, as well as secreted, both IL-2 and IL-4 thus confirming the clonality of the original T cell clones. CD4+ T cell clones that expressed an unrestricted cytokine profile upon Con A stimulation were also isolated from mice immunized to other Ag (hen egg lysozyme, OVA, or type II collagen). These data indicate that CD4+ T cell clones newly isolated from immunized mice do not necessarily segregate into the Th1 and Th2 subsets. We propose this new murine CD4+ cell subset with an unrestricted pattern of cytokine production be called Th0.

Animals↗

Gene 19 of bacteriophage T7. Overexpression, purification, and characterization of its product.

Gene 18 and 19 proteins of bacteriophage T7 are essential for DNA maturation and packaging. The phage capsid is the site of both maturation and packaging of T7 DNA. Both gene 18 and 19 proteins bind to capsid intermediates during DNA packaging but are not found in mature virions, suggesting that they play a direct role in the enzymatic mechanisms of DNA maturation and packaging. As part of an effort to reconstitute T7 DNA maturation and packaging with purified components, we have cloned and overexpressed T7 gene 19 in Escherichia coli. Gene 19 has been inserted downstream from the bacteriophage PL promoter controlled by the temperature-sensitive lambda repressor encoded by c1857. Upon thermal induction, most of the overproduced gene 19 protein is insoluble and inactive. However, by attenuation of the expression of gene 19 from the PL promoter, significant levels of soluble and active gene 19 protein are produced. Soluble gene 19 protein can be monitored by its ability to complement extracts of T7-infected cells for packaging of exogenous DNA. We have used this assay to monitor the activity of gene 19 protein during purification. The native protein is a monomer of molecular weight 66,000. We have also tested for the formation of a stable complex between gene 18 and 19 proteins. Coproduction of gene 18 and 19 proteins has no effect on either the solubility or activity of gene 19 protein, despite the fact that gene 18 protein is produced at at least 10-fold greater rates. Furthermore, we find no evidence for any interaction between soluble gene 18 and 19 proteins in extracts or between the purified proteins.

Electrophoresis, Polyacrylamide Gel↗

[Behavior of the large fragment of DNA polymerase I (the Klenow fragment) during fractionation of a cell-free extract of E. coli MRE-600].

Distribution of the DNA polymerase I large fragment (Klenow fragment) was studied during fractionation of the E. coli MRE-600 cell-free extract with polyethylenimine. On the basis of the results obtained a simple procedure is proposed that enables the Klenow fragment to be obtained as a coproduct of DNA polymerase I, RNA polymerase, polynucleotide phosphorylase, nucleotide kinases with acetokinase and nucleoside deoxy-ribosyltransferase in the framework of a combined technological scheme.

Chemical Fractionation↗

Inhibition of growth but not differentiation of normal and leukemic myeloid cells by methylthioadenosine.

Methylthioadenosine (MTA), a coproduct of polyamine biosynthesis, is known to inhibit proliferation in a variety of cell culture systems. In this paper, we show that while MTA inhibits the growth of the human promyelocytic cell line HL-60, it does not interfere with retinoic acid-induced granulocytic or phorbol ester-induced monocytic differentiation of these cells. MTA also inhibits proliferation induced by colony stimulating activity of normal human granulocytic precursor cells grown in suspension culture but does not suppress terminal differentiation of these cells. In contrast to the lack of effect of MTA on granulocytic differentiation which we report here, others have shown that MTA prevents terminal differentiation of murine erythroleukemia cells. That MTA is a normal cellular constituent which inhibits proliferation but not differentiation of normal granulopoietic cells and may have opposing effects on immature cells of erythroid lineage suggests a possible role for this compound in the regulation of hematopoiesis. In addition, MTA may be useful for studying the process of differentiation in the absence of cell proliferation in granulopoietic cells.

Adenosine↗

Styrene use and occupational exposure in the plastics industry.

The commercial production of styrene was started in the 1930s. Currently 7 million tons of styrene a year is made worldwide by the catalytic dehydrogenation of ethylbenzene or by a process in which styrene is produced as a coproduct with propylene oxide. An estimated 62% of the styrene monomer is consumed in the manufacture of polystyrene, 12% in acrylonitrile-butadiene-styrene resins, 17% in styrene-butadiene rubber and latex, 7% in unsaturated polyester resins, and 2% in other applications. Occupational exposure to styrene occurs in monomer production and polymerization plants, during the fabrication of plastic products from monomeric or partly prepolymerized styrene, and during the transportation and handling of liquid styrene. Due to unreacted residual monomer or thermal degradation of the polymer, low concentrations of styrene can be detected during the use of plastic products. The most extensive and intensive exposure occurs in the reinforced plastics industry, in which over 200,000 workers are exposed to a styrene concentration typically ranging from 20 to 300 ppm.

Air Pollutants↗

Coinduction of nitric oxide synthase and argininosuccinate synthetase in a murine macrophage cell line. Implications for regulation of nitric oxide production.

In macrophages and other cell types, bacterial lipopolysaccharide and certain cytokines stimulate nitric oxide (NO) production via expression of the inducible isoform of nitric oxide synthase (NOS). Citrulline, which is the coproduct of NOS-catalyzed metabolism of arginine, can be recycled to arginine by the action of argininosuccinate synthetase and argininosuccinate lyase, which are present at high levels in hepatocytes and renal tubular cells but normally at very low levels in other cell types such as macrophages. The present study demonstrates that lipopolysaccharide and interferon-gamma, which induce NOS in the murine macrophage cell line RAW 264.7, also coinduce activity and mRNA for argininosuccinate synthetase, which is limiting for arginine synthesis. Argininosuccinate lyase activity and mRNA abundance are unaffected. Induction of argininosuccinate synthetase is not blocked by NG-monomethyl-L-arginine, a potent inhibitor of NOS, indicating that argininosuccinate synthetase induction is not the consequence of depleting cellular arginine levels by NOS. Because plasma levels of arginine are limiting for NO synthesis, enhanced cellular capacity to regenerate arginine from citrulline could play a significant role in regulating NO production, especially under conditions where the inducible isoform of NOS is expressed.

Amino Acid Oxidoreductases↗

Sodium nitroprusside evokes the release of immunoreactive calcitonin gene-related peptide and substance P from dorsal horn slices via nitric oxide-dependent and nitric oxide-independent mechanisms.

The results of behavioral studies suggest that nitric oxide (NO) participates in certain spinal mechanisms that contribute to hyperalgesia. Additionally, previous studies indicate that the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P (iSP) is increased in the dorsal horn of the spinal cord during hyperalgesia. Therefore, the aim of this study was to determine whether NO acts to enhance peptide release in the dorsal horn of rats using an in vitro superfusion technique. Sodium nitroprusside (SNP) was used as an NO donor. The results of this study indicate that SNP caused a dose-related, calcium-dependent increase in the release of iCGRP and iSP from dorsal horn slices of the rat spinal cord. Furthermore, pretreatment with SNP reduced the ability of capsaicin to evoke the release of either peptide, suggesting that a target for SNP exists on certain capsaicin-sensitive primary afferent terminals. In addition to increasing peptide release, SNP also caused a significant five to sixfold increase in the levels of immunoreactive guanosine 3',5'-monophosphate (i-cGMP) in the dorsal horn. This SNP-evoked increase was significantly decreased by the guanylate cyclase inhibitor methylene blue in a dose-dependent manner. In addition, the release of iCGRP was also significantly reduced in the presence of methylene blue, although the relationship between peptide release and i-cGMP production remains unclear. Sodium nitroprusside-evoked peptide release was significantly reduced in the presence of hemoglobin (an oxide radical scavenger), suggesting that the drug effect was due to the generation of NO. However, the release of iCGRP and iSP was also evoked by sodium ferricyanide (the coproduct of SNP) and by 7-d-old, photoinactivated SNP.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗