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Results for “Complement Fixation Tests”

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At least 235 records · Page 13Linked to original sources
PubMed1991

[New human polyomavirus: comparison of hemagglutination inhibition, ELISA and complement fixation tests for BK virus serology].

In this study, 110 sera samples taken from healthy persons were tested for BK virus antibodies by using hemagglutination inhibition (HI), ELISA and complement fixation (CF) tests to detect the sensitivity, specificity and agreement of these tests. The results of each individual test was compared with others for sensitivity and specificity. Specificity found to be 96% when HI and CF tests were compared. Sensitivity also found to be 92.7% when CF and ELISA tests were compared. Agreement of HI and ELISA tests were 88%.

Antibodies, Viral↗
PubMed1985

Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of Campylobacter jejuni antibodies, and comparison with a complement fixation test (CFT).

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of total anti-Campylobacter immunoglobulins in human sera. In this assay disintegrated Campylobacter bacteria were used as the antigen. Absorption tests including other possibly enteropathogenic bacterial species showed that the ELISA system displayed a high immunological specificity for Campylobacter. Using this ELISA it was found that in about 80% of Campylobacter patients these Campylobacter antibodies are produced to almost maximal levels within 8 days after onset of disease, and that they may persist for at least 4 months. Indeed, Campylobacter antibodies were demonstrated at low levels in a large number of control sera. However, accepting an antibody titre of 1:640 as indicative of Campylobacter infection, the statistical sensitivity of the ELISA system was 77% and the specificity 95%. In an epidemiological survey a high association was demonstrated between the severity of Campylobacter-related symptoms and antibody titre values. Assessment of Campylobacter antibody titres by means of this ELISA and by a complement fixation test in 92 sera from index patients and contacts with and without symptoms showed a high association of results.

Antibodies, Bacterial↗
PubMed1967

Methods of preparing supplementing fractions from fresh unheated bovine sera for use in modified direct complement-fixation tests.

TWO METHODS OF PREPARING PARTIALLY PURIFIED, RELATIVELY STABLE SUPPLEMENTING FACTOR FROM FRESH BOVINE SERUM ARE DESCRIBED: gel filtration through Sephadex G-25, and anion exchange chromatography on a diethylaminoethyl (DEAE) cellulose column. In both procedures, an active, reconstituted precipitate prepared by dialysis of fresh unheated normal serum in the cold for 18 hours against phosphate buffer pH 6.2, 0.02 M, serves as the starting material. The Sephadex G-25 column is equilibrated with acetate buffer pH 5.4, 0.2 M. The most actively-supplementing material appears in the eluates in which the pH has risen to 7.5 or higher. For the DEAE cellulose chromatography a gradient system is used: initial phosphate buffer 0.03 M, pH 8.0, limiting buffer Na H(2)PO(4), 0.3 M. The greater part of the supplementing activity is eluated between pH 5.6 and 6.0, although some of the earlier fractions are also reactive. Pooled active eluates stored in the frozen state for nine months or longer maintained their supplementing titre in modified complement-fixation tests of two bacterial antigen-bovine antibody systems.

Animals↗
PubMed1987

Single radial complement fixation test using NP-containing plates: a simple and sensitive method for the detection of influenza infection.

The single radial complement fixation (SRCF) test using nucleoprotein (NP) was compared with the haemagglutination inhibition (HAI) test in the course of the evaluation of the protective efficacy of influenza vaccine. In the case of persons who were not vaccinated, the percent incidence of infection was almost the same (which confirmed by HAI test or by SRCF test), whereas in the case of vaccinees, there was a significant difference between the incidence confirmed by HAI (7.9%) and SRCF (19.4%) tests. The SRCF test was shown to be a simple and sensitive method for detection of a significant rise in antibody titre against influenza virus.

Antibodies, Viral↗
PubMed1981

Complement fixation tests for equine piroplasmosis (Babesia equi and B caballi) performed in the UK during 1976 to 1979.

The results of complement fixation (CF) test for equine piroplasmosis on sera from horses destined for international movement from Great Britain and Ireland are presented and analysed. No horses born and continuously resident in the British Isles were found carrying CF antibodies to either Babesia equi or B caballi. Positive animals were found to have association with the following countries where known tick vectors occur: Spain, Portugal, Belgium, France, Poland, USSR and Arabian Gulf countries. Data on the persistence of CF antibodies in animals subjected to repeated testing showed that some animals lost their CF titre within a few months of their first test while others retained a titre for periods up to 13 months of B caballi and 28 months for B equi. Some individuals regained titres on subsequent tests after becoming negative and the results suggest that removal from exposure to infection or, even, treatment does not necessarily eliminate a CF titre within a predictable time limit.

Animals↗