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[Formation of cellular structures and interneuronal connections in an organotypic culture of newborn mouse hippocampal tissue].

The cytoarchitectonic structures and fibres pathways of the newborn mouse hippocampus were studied in vitro. After 2-3 weeks in vitro it was possible to see on transverse slices of the hippocampus main fascicles (alvear tract, perforant path, axons of granular cells) and cytoarchitectonic formations (pyramidal cell layer of hippocampus, infra- and supra-pyramidal lymbs of dentate gyrus granular cells). The postnatal processes of histogenesis are suggested to result in subsequent differentiation of the cytoarchitectonic formations and in the systemic growth of nerve fibres in the hippocampus explants. Thus, organo-typic explants of the hippocampus represent a useful model systems for electrophysiological studies in systemic interneuronal connections formed de novo as a result of postnatal development in vitro.

Animals↗

[Possibility of the development of cellular structures on the surface of a vascular plexus demonstrated by scanning electron microscopy].

Using scanning electron microscopy, possible ways of the appearance of macrophages on the epithelial surface of the cerebral vascular plexus in rats were studied. The data obtained support the theory which postulates that macrophages are formed in the blood flow or the connective tissue and that subsequently they penetrate through the epithelium of the vascular plexus onto its surface having contact of the cerebrospinal fluid. The structural stages of this process are also reflected in the presented findings.

Animals↗