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PubMed1990

Chromatographic determination of amines in biological fluids with special reference to the biological monitoring of isocyanates and amines. IV. Determination of 1,6-hexamethylenediamine in human urine using capillary gas chromatography and selective ion monitoring.

A capillary gas chromatographic (GC) method was developed for the determination of 1,6-hexamethylenediamine (HDA) in hydrolysed human urine. The method was based on a derivatization procedure with heptafluorobutyric anhydride. The amides formed were determined using capillary GC with selected ion monitoring in the chemical ionization mode with ammonia as reagent gas. The overall recovery was 34% for a concentration of 100 micrograms/l of HDA in urine. The minimum detectable concentration in urine was below 0.5 microgram/l. The precision of the method was 5% (n = 9). Deuterium-labelled HDA [H2NC2H2(CH2)4C2H2NH2] was used as the internal standard. A male subject was exposed to hexamethylene diisocyanate (HDI) for 7.5 h in a test chamber. The average air concentration of HDI was ca. 30 micrograms/m3, which corresponds to ca. 85% of the threshold limit value in Sweden (35 micrograms/m3). The half time of urinary levels of HDA was ca. 1.4 h and more than 90% of the urinary elimination was completed within 4 h after the exposure. The amount of HDA excreted in urine was ca. 10 micrograms, corresponding to ca. 10% of the estimated inhaled dose of HDI.

Amines↗
PubMed2002

[Various mammalian tissues contain cell membrane-bound amine oxidase termed semicarbazide-sensitive amine oxidase (SSAO, EC 1.4.3.6)].

Recently it has been demonstrated a role of fat-cell SSAO on glucose transport and GLUT4 translocation to the cell surface. Many compounds have been identified as relatively selective SSAO inhibitors, but those currently available also inhibit monoamine oxidase (MAO, EC 1.4.3.4). In this study, inhibitory properties of a haloamine, 2-bromoethylamine (2-BEA), to guinea pig and rat lung-bound SSAO have been studied. 2-BEA could not inhibit both forms of MAO, but it competitively inhibited rat lung SSAO activity with a Ki value of 2.5 microM without preincubation and after preincubation, the mode of inhibition changed to be non-competitive. Dialysis and dilution experiments with 2-BEA-pretreated preparations resulted in no recovery of SSAO activity. A decreased rate of SSAO inhibition under N2 atmosphere to that obtained under O2 was produced after 2-BEA treatment, suggesting that oxidised intermediate was necessary for its inhibition. The plot of 1/k' vs 1/2-BEA intersected on the y-axis indicate that the inhibition by 2-BEA is not affinity-labeling agent, but a suicide inhibitor of SSAO.

Amine Oxidase (Copper-Containing)↗