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At least 217 records · Page 12Linked to original sources

Mainstream smoke constituent yields and predicting relationships from a worldwide market sample of cigarette brands: ISO smoking conditions.

The study objective is evaluation of a benchmark approach for predicting mainstream smoke constituent machine-yields for conventional cigarette brands from worldwide markets. Results for ISO smoke yields support the validity of benchmarking when brands, for which yields are to be predicted, have design characteristics within boundaries established by the exploratory brands. Yields of ISO-method mainstream smoke constituents were generally well described by weighted least squares regression relationships with ISO tar (R2>0.80 and coefficient p values <0.05). The impact of the varied chemical composition of cigarette tobaccos from different regions on smoke constituent yields was recognized. Mainstream smoke nitrogen oxides and tobacco-specific nitrosamine (TSNA) yield prediction relationships improved by including tobacco nitrate or TSNA concentration factors in respective independent parameters. For carbon-filter brands, inclusion of a carbon factor improved the predicting relationships for several vapor-phase constituents. Relationships were validated with a subset of additional validation brands. Greater than 90% of the validation brands' smoke chemistry yields were within the 95% prediction intervals. Average differences between measured and predicted yields were generally within the range of one to two measurement standard deviations. The estimation methods proposed relate to machine-smoking conditions and are not intended to reflect the actual exposure of any given consumer to smoke constituents.

Benchmarking↗

Estimating biomass yield coefficients for autotrophic ammonia and nitrite oxidation from batch respirograms.

Kinetic characterization of biological processes via batch respirometry requires an accurate estimate of the biomass yield coefficient because it provides the stoichiometric link between biomass synthesis, substrate consumption and oxygen uptake. Expressions for biomass yield coefficients describing autotrophic ammonia and nitrite oxidation were derived from a mechanistically based electron balanced equation. We demonstrate that applying the conventional expression used to calculate the heterotrophic biomass yield results in erroneous estimates for the autotrophic biomass yield. Yield coefficients for autotrophic NH4(+)-N to NO2(-)-N oxidation and NH4(+)-N to NO3(-)-N oxidation were overestimated by 27 to 36%. Due to correlation between the maximum specific growth rate and the biomass yield, the error in yield values propagated in 30 to 40% overestimates of the maximum specific growth rate coefficient for NH4(+)-N oxidation determined from batch respirograms. Therefore, it is essential to employ the correct expression to estimate the autotrophic biomass yield coefficient from batch respirograms due its inadvertent impact on subsequent parameter estimation.

Ammonia↗

[Factors related to diagnostic yield and complications of transbronchial biopsy].

To study the diagnostic yield of transbronchial biopsy (TBB) in lung diseases of different ethiology, as well as to examine several factors implicated in diagnostic yield and complications of TBB. Retrospective study of a series of 172 patients (98 women and 74 men) undergoing TBB. Clinical variables, x-ray and CT patterns and technical factors related to TBB were analyzed in relation to diagnostic yield and definitive diagnosis. We recorded the main complications and studied the possible mechanisms implicated in their appearance. The results of TBB were classified as follows: 1) diagnostic TBB (42.8%); 2) nonspecific TBB (21.1%); absence of parenchyma (NP) (9.7%); 4) normal parenchyma (23.4%); 5) incorrect diagnosis (2.9%). Overall yield was 43.6% but rose to 52% when NP and no infiltrative pneumopathies were excluded. The main findings were infections (23.4%), neoplasms (19.4%), sarcoidosis (14.2%), idiopathic pulmonary fibrosis (IPF) (17.7%); and other (23.4%). Significant differences in diagnosis were found for age, sex, time of evolution prior to TBB, and x-ray and CT patterns. Complications and pneumothorax appeared more frequently in IPF, neoplasms and infections, although the differences were non significant. Diagnostic value depended mainly on type of disease and ranged from 60% for sarcoidosis to 24% for IPF (p < 0.05), with no differences related to age, sex, presence of immunosuppression, number of TBB or tolerance to exploration. Yield was higher, however, for patients with no loss of radiographic pulmonary volume (56.3% versus 37.5%) and with absence of a CT reticular pattern (44.7% versus 27.3%). Type of opacites also influenced yield (53.4% for ground glass versus 26.7% for reticular patterns), as did distribution of lesions (8.4% for peripheral patterns versus 48.3% for diffuse patterns and 50% for peribronchio-vascular patterns). The site of TBB was the only factor showing clearly significant differences in yield (100% in mild lobe versus 29.5% in upper lobes). Tolerance was poorer in older patients (63.5 +/- 10 versus 52.2 +/- 17 years) and with greater function abnormalities (%FEV1: 48.2 +/- 16.8 versus 70.2 +/- 17.1). Poor tolerance of exploration was associated with the appearance of complications (16.6% vs 6.3) and pneumothorax (25% versus 6.8%). The diagnostic yield of TBB was mainly influenced by the type of disease studied. The various imaging techniques were fundamental for establishing preliminary diagnoses and degree of evolution of a specific cases. In our series, tolerance of the technique was a reliable predictor of complications and the presence of post-biops pneumothorax.

Adult↗

Mapping QTL for biomass yield and its components in rice (Oryza sativa L.).

Additive effects, additive by additive epistatic effects, and their environmental interactions of QTLs are important genetic components of quantitative traits. Genetic architecture underlying rice biomass yield and its two component traits (straw yield and grain yield) were analyzed for a population of 125 DH lines from an inter-subspecific cross of IR64/Azucena. The mixed-model based composite interval mapping approach (MCIM) was used to detect QTLs, There were 12 QTLs detected with additive main effects, 27 QTLs involved in digenic interaction with aa and/or aae effects, and 18 QTLs affected by environments with ae and/or aae effects. It was revealed that epistatic effects and QE interaction effects existed on biomass yield and its component traits in rice. In addition, the genetic basis of relationships among these traits were investigated. Four QTLs and one pair of epistatic QTLs were detected to be responsible for the positive correlation between biomass yield and straw yield. Three QTLs might be responsible for the negative correlation between straw yield and grain yield. This result could partially explain the genetic basis of correlation among the three traits, and provide useful information for genetic improvement of these traits by marker-assisted selection.

Biomass↗

High-yield production of hydrogen by Enterobacter aerogenes mutants with decreased alpha-acetolactate synthase activity.

To enhance hydrogen (H2) production from glucose by Enterobacter aerogenes HU-101, two mutants, strains VP-1 and VP-2, with decreased alpha-acetolactate synthase activity, were isolated using the Voges-Proskauer (VP) test. In pH-uncontrolled batch culture, both mutants showed a lower 2,3-butanediol yield for the glucose consumed than that shown by the wild-type strain, although glucose remained in the medium after 12 h of culture. In the same cultures, compared to the H2 yield of 0.80 mol/mol-glucose of the wild-type strain, strain VP-1 showed a high H2 yield of 1.8 mol/mol-glucose with decreased lactate and increased succinate yields, while strain VP-2 showed an H2 yield of 1.0 mol/mol-glucose with an increased lactate yield. Increasing the phosphate buffer concentration, which contributes to maintaining the pH in the medium, increased the glucose consumption by both strains. However, in a pH-controlled batch culture at neutral pH, the H2 yield of strain VP-1 was decreased to 1.2 mol/mol-glucose due to the accumulation of formate, an intermediate of the H2-producing pathway, with the yield of H2 plus formate being 1.7 mol/mol-glucose.

Journal Article↗

Short-term associations between disease and milk yield of dairy cows.

Short-term associations between disease and milk yield were studied in 1594 lactations occurring between 1977 and 1982 in 732 Friesian, Ayrshire and Holstein crossbred cows. Statistically significant differences in milk yield between one week before and one week after clinical diagnosis were observed for ketosis (5.1 kg/d), hypomagnesaemia (4.1 kg/d), mastitis occurring after peak yield (2.1 kg/d) and lameness (1.1 kg/d). Milk yield declined for as much as 2-4 weeks before diagnosis of disease, and total losses in milk yield associated with ketosis or hypomagnesaemia were estimated to be 60-70 kg. No significant differences were found for endometritis or for mastitis when it occurred before peak yield. Milk yield was suppressed for about 4 weeks after calvings with retained placenta, but there were no corresponding effects of dystocia or hypocalcaemia on milk yield. It is suggested that if automatic daily milk recording is available it may be possible to detect deviations from normal, and hence detect subclinical disease 2 or more weeks before its clinical appearance.

Animals↗

Nonphotochemical quenching of excitation energy in photosystem II. A picosecond time-resolved study of the low yield of chlorophyll a fluorescence induced by single-turnover flash in isolated spinach thylakoids.

Chlorophyll a fluorescence emission is widely used as a noninvasive measure of a number of parameters related to photosynthetic efficiency in oxygenic photosynthetic organisms. The most important component for the estimation of photochemistry is the relative increase in fluorescence yield between dark-adapted samples which have a maximal capacity for photochemistry and a minimal fluorescence yield (F0) and light-saturated samples where photochemistry is saturated and fluorescence yield is maximal (Fm). However, when photosynthesis is saturated with a short (less than 50 micro(s)) flash of light, which induces only one photochemical turnover of photosystem II, the maximal fluorescence yield is significantly lower (Fsat) than when saturation is achieved with a millisecond duration multiturnover flash (Fm). To investigate the origins of the difference in fluorescence yield between these two conditions, our time-resolved fluorescence apparatus was modified to allow collection of picosecond time-resolved decay kinetics over a short time window immediately following a saturating single-turnover flash (Fsat) as well as after a multiturnover saturating pulse (Fm). Our data were analyzed with a global kinetic model based on an exciton radical pair equilibrium model for photosystem II. The difference between Fm and Fsat was modeled well by changing only the rate constant for quenching of excitation energy in the antenna of photosystem II. An antenna-based origin for the quenching was verified experimentally by the observation that addition of the antenna quencher 5-hydroxy-1,4-naphthoquinone to thylakoids under Fm conditions resulted in decay kinetics and modeled kinetic parameters very similar to those observed under Fsat conditions in the absence of added quinone. Our data strongly support the origin of low fluorescence yield at Fsat to be an antenna-based nonphotochemical quenching of excitation energy in photosystem II which has not usually been considered explicitly in calculations of photochemical and nonphotochemical quenching parameters. The implications of our data with respect to kinetic models for the excited-state dynamics of photosystem II and the practical applications of the fluorescence yield parameters Fm and Fsat to calculations of photochemical yield are discussed.

Chlorophyll↗

Cell yield and cell survival following chemotherapy of the B16 melanoma.

We describe in this paper cell survival studies, using in vitro clonogenic assays, performed on the B16 melanoma treated in situ with various cytotoxic agents. In addition we have determined the effects of these agents on the yield of cells obtained by trypsinization. In untreated tumours the mean cell yield was approximately 10(8)/g, which is 20--30% of the cells actually present in the tissue. The plating efficiency was approximately 40%. Most agents rapidly affected both cell yield and cell survival. For example, within 20--30 h, gamma-radiation and several alkylating agents reduced cell yield by about 40%. The cell yield change was associated with an increase in mean cell size. Cell yield was reduced even more (approximately 70%) by Vinca alkaloids. This large reduction was associated with extensive cell lysis, observed as an increase in the necrotic fraction of tumours from approximately 35% to approximately 70%. Adriamycin, bleomycin and Ara-C also produced a moderate reduction in cell yield (approximately 40%), but actinomycin D did not reduce cell yield and FU increased it by about 30%. Only gamma-radiation, cyclophosphamide, CCNU, BCNU and melphalan produced more than a 90% reduction in cell survival, although there was a small but measurable reduction with all other agents except vinblastine, HN2 and actinomycin D.

Alkylating Agents↗

Nicotine and carbon monoxide intake from high- and low-yield cigarettes.

We measured daily nicotine intake in 11 habitual smokers who smoked their usual brand or commercial high-yield (Federal Trade Commission [FTC] yield 1.2 mg nicotine, 16 mg tar) or low-yield (0.4 mg nicotine, 5 mg tar) cigarettes. Daily nicotine intake was measured from metabolic clearance data in conjunction with blood and urinary concentrations of nicotine during 24-hr smoking periods. On the average, subjects consumed 35 mg nicotine while smoking their usual cigarettes and 26 mg while smoking either high- or low-yield commercial cigarettes different from their usual brand. This level of nicotine consumption from low-yield cigarettes was because smokers obtained 60% more nicotine per cigarette than predicted by FTC yield and they smoked 25% more cigarettes a day. Although there was considerable variability in nicotine intake between subjects, there was a correlation within subjects between intake while smoking their usual brand and experimental cigarettes. Nicotine intake between the two commercial high- or low-yield cigarettes also correlated (r = 0.86). These findings are consistent with a minimal level of acceptable daily intake of nicotine for individuals that is related to usual intake. We suggest that our protocol provides a better quantitative estimate of the yield of different cigarette brands and potential health hazards than those currently provided by the FTC.

Adult↗

The yield and nutrient content of colostrum and milk of women from giving birth to 1 month post-partum.

The intake of mammary secretion from delivery to day 5 post-partum was determined by test-weighing nine infants using an integrating electronic balance. The mean yield of colostrum for the first 24 h after birth was 37.1 (range 7.0-122.5) g and was 408 (range 98.3-775) and 705.4 (range 452.5-876) g/24 h on days 3 and 5 post-partum respectively. The milk yield of mothers on either day 14 or 28 post-partum was determined by test-weighing the mother. The mean milk yield was 1.156 (SD 0.167) kg/24 h. A significant correlation (P less than 0.001; r 0.85, n 42) was found between milk yield measured by test-weighing the infant and milk yield measured by test-weighing the mother, confirming that it is possible to obtain a similar estimate of milk consumed using either of the two methods of test-weighing. There was a significant positive correlation (P less than 0.001) between lactose concentration and milk yield for the first 5 d post-partum (r 0.76, n 22); a significant correlation (P less than 0.001) between protein concentration and milk yield (r-0.74, n 22) and no significant correlation between fat concentration and milk yield for the period studied. The calculated energy intake of infants during the first 24 h after birth was only 0.12 (range 0.02-0.29) mJ. This increased to 1.44 (range 0.83-2.18) and 2.99 (range 2.49-4.06) mJ/24 h by days 3 and 14-28 post-partum respectively.

Body Weight↗

Yields of radiation-induced base products in DNA: effects of DNA conformation and gassing conditions.

Gas chromatography-mass spectrometry with selected-ion monitoring was used to measure the yields of radiation-induced base products in aqueous solutions of native or heat-denatured DNA irradiated in the dose range 20-100 Gy. These DNA solutions were saturated with nitrous oxide, nitrogen, air or 20% oxygen in nitrous oxide during irradiation. The products measured were as follows: 5,6-dihydrothymine; 5-hydroxy-5,6-dihydrothymine; 5,6-dihydrothymine (thymine glycol); 5-hydroxy-5,6-dihydrocytosine; 5,6-dihydroxy-5,6- dihydrocytosine (cytosine glycol); 4,6-diamino-5-formamidopyrimidine; 7,8-dihydro-8-oxoadenine (8-hydroxyadenine); 2,6-diamino-4-hydroxy-5- formamidopyrimidine; and 7,8-dihydro-8-oxoguanine (8-hydroxyguanine). In oxygenated solutions, 5,6-dihydrothymine, 5-hydroxy-5,6-dihydrothymine and 5-hydroxy-5,6-dihydrocytosine were not formed. The yields of all products, other than 5,6-dihydrothymine, were greater in irradiated DNA samples from N2O-saturated solutions than from N2-saturated solutions. In N2-saturated solutions the yield of 8-hydroxyadenine was low and 8-hydroxyguanine was undetectable. Yields of pyrimidine products in heat-denatured DNA were greater than those in native DNA using all types of gases. However, the effects of DNA conformation on the yields of purine products were dependent on the type of gas used to saturate the irradiated DNA solutions. Yields of formamidopyrimidines were generally lower in solutions of DNA irradiated in the native than in the heat-denatured conformation. In air-saturated solutions of DNA, yields of 8-hydroxypurines were not influenced greatly by DNA conformation. In DNA solutions saturated with N2O/O2, 8-hydroxypurine formation was more favourable in the heat-denatured conformation than in the native conformation. On the other hand, in deoxygenated solutions, formation of 8-hydroxypurines was favoured in the native conformation. Data indicate that DNA conformation and the type of gas used to saturate the irradiated solutions have a profound influence on yields of base products in DNA.

Air↗

Estimating the hazards of "less hazardous" cigarettes. III. A study of the effect of various smoking conditions on yields of hydrogen cyanide and cigarette tar.

A brand of cigarette with nominal tar and nicotine yields of 4.0 mg and 0.4 mg, respectively, was examined under various machine-smoked conditions that reflect the wide range of human smoking behavior. Three levels of each of five smoking parameters--butt length, puff duration, puff interval, puff volume, and ventilation occlusion--were examined, and the effects on puff number and total particulate matter (TPM) as well as gas phase, particulate phase, and total HCN yields were estimated. Yields of hydrogen cyanide (a ciliatoxic agent) and TPM varied significantly under different smoking conditions. Ventilation occlusion had the most pronounced effect, accounting for 34% of the response variation of TPM yields and 42% of the response variation for total hydrogen cyanide yields. In the survey of available brands, total hydrogen cyanide yields varied from 2 to 233 micrograms/cigarette for the 115 brands tested, a range less than that observed for a single brand smoked under various nonstandard conditions, providing a possible explanation for the previously noted lack of correspondence between HCN yields under standard conditions and levels of thiocyanate in samples of smokers' plasma and saliva. In addition, hydrogen cyanide yields and efficiency of filters in removing HCN were examined under standard smoking conditions. Acetate filters, the most common variety, were found to remove an average of about 14% of the hydrogen cyanide from the mainstream smoke.

Analysis of Variance↗

Definition of architectural ideotypes for good yield capacity in Coffea canephora.

BACKGROUND: Yield capacity is a target trait for selection of agronomically desirable lines; it is preferred to simple yields recorded over different harvests. Yield capacity is derived using certain architectural parameters used to measure the components of yield capacity. METHODS: Observation protocols for describing architecture and yield capacity were applied to six clones of coffee trees (Coffea canephora) in a comparative trial. The observations were used to establish architectural databases, which were explored using AMAPmod, a software dedicated to the analyses of plant architecture data. The traits extracted from the database were used to identify architectural parameters for predicting the yield of the plant material studied. CONCLUSIONS: Architectural traits are highly heritable and some display strong genetic correlations with cumulated yield. In particular, the proportion of fruiting nodes at plagiotropic level 15 counting from the top of the tree proved to be a good predictor of yield over two fruiting cycles.

Agriculture↗

Effects of age, sex, and duration of postmortem aging on percentage yield of parts from broiler chicken carcasses.

The objective of this study was to evaluate effects of age, sex, and postmortem carcass aging duration on parts yield from broiler chickens. Two hundred twenty-four mixed-sex broilers were reared under commercial-like conditions for various periods between 37 and 51 d, slaughtered, packed in ice, and then aged for 0, 2, 4, or 6 h. Mean percentage yield of thighs, drumsticks, forequarters, wings, breasts, and filets were evaluated for each rearing period, sex, and postmortem aging duration. Yield of meatier parts such as thighs, forequarters, breasts, and filets increased with birds' ages. Female carcasses produced higher percentage yields of forequarters, breasts, and filets but lower yields of drumsticks. Carcasses aged 2 h or more postmortem tended to have lower yields of forequarters, breasts, and drumsticks than did carcasses aged for shorter durations. No statistically significant interactions among age, sex, or postmortem aging duration that affected yield of parts were detected. This information is useful to integrated poultry firms wishing to optimize yield of the most commercially valuable parts.

Age Factors↗

Quantum Yields for CO(2) Uptake in C(3) and C(4) Plants: Dependence on Temperature, CO(2), and O(2) Concentration.

The quantum yields of C(3) and C(4) plants from a number of genera and families as well as from ecologically diverse habitats were measured in normal air of 21% O(2) and in 2% O(2). At 30 C, the quantum yields of C(3) plants averaged 0.0524 +/- 0.0014 mol CO(2)/absorbed einstein and 0.0733 +/- 0.0008 mol CO(2)/absorbed einstein under 21 and 2% O(2). At 30 C, the quantum yields of C(4) plants averaged 0.0534 +/- 0.0009 mol CO(2)/absorbed einstein and 0.0538 +/- 0.0011 mol CO(2)/absorbed einstein under 21 and 2% O(2). At 21% O(2), the quantum yield of a C(3) plant is shown to be strongly dependent on both the intercellular CO(2) concentration and leaf temperature. The quantum yield of a C(4) plant, which is independent of the intercellular CO(2) concentration, is shown to be independent of leaf temperature over the ranges measured. The changes in the quantum yields of C(3) plants are due to changes in the O(2) inhibition. The evolutionary significance of the CO(2) dependence of the quantum yield in C(3) plants and the ecological significance of the temperature effects on the quantum yields of C(3) and C(4) plants are discussed.

Journal Article↗

Effects of O(2) and CO(2) Concentrations on Quantum Yields of Photosystems I and II in Tobacco Leaf Tissue.

The interactive effects of irradiance and O(2) and CO(2) levels on the quantum yields of photosystems I and II have been studied under steady-state conditions at 25 degrees C in leaf tissue of tobacco (Nicotiana tabacum). Assessment of radiant energy utilization in photosystem II was based on changes in chlorophyll fluorescence yield excited by a weak measuring beam of modulated red light. Independent estimates of photosystem I quantum yield were based on the light-dark in vivo absorbance change at 830 nanometers, the absorption band of P700(+). Normal (i.e. 20.5%, v/v) levels of O(2) generally enhanced photosystem II quantum yield relative to that measured under 1.6% O(2) as the irradiance approached saturation. Photorespiration is suspected to mediate such positive effects of O(2) through increases in the availability of CO(2) and recycling of orthophosphate. Conversely, at low intercellular CO(2) concentrations, 41.2% O(2) was associated with lower photosystem II quantum yield compared with that observed at 20.5% O(2). Inhibitory effects of 41.2% O(2) may occur in response to negative feedback on photosystem II arising from a build-up in the thylakoid proton gradient during electron transport to O(2). Covariation between quantum yields of photosystems I and II was not affected by concentrations of either O(2) or CO(2). The dependence of quantum yield of electron transport to CO(2) measured by gas exchange upon photosystem II quantum yield as determined by fluorescence was unaffected by CO(2) concentration.

Journal Article↗

Factors influencing yield of plateletpheresis using intermittent flow cell separator.

Platelet recovery in the recipient is influenced by the transfused dose of platelets, which in turn is dependent on the quality of single donor platelets (SDPs) in terms of platelet yield. Various donor factors such as predonation platelet count and Hemoglobin (Hb) concentration affect the platelet yield. A total of 61 plateletpheresis procedures performed on intermittent flow cell separator (MCS3p, Hemonetics) were evaluated for platelet yield. A relationship between predonation platelet count and Hb concentration with yield of platelets was studied using Pearson Correlation. The mean platelet yield was 2.9 +/- 0.64 x 10(11). While a direct relationship was observed between predonation platelet count and yield (r = 0.51, P < 0.001), no such correlation was noticed with donor Hb concentration (r = -0.05, P > 0.005). The yield was > or =3 x 10(11) in >80% of procedures when the predonation platelet count was > or =250 x 10(3)/mm. Optimization of platelet yield, which is influenced by predonation platelet count, is an emerging issue in blood transfusion services. However, further studies in this regard are needed using more advanced cell separators.

Adolescent↗

Yield strength of zirconia and glass fibre-reinforced posts.

The aim of this in vitro study was to evaluate the yield strengths of glass fibre-reinforced composite (FRC) posts and zirconia dioxide ceramic (ZDC) posts. Tapered glass FRC posts (DentinPost) and ZDC posts (Cerapost) of three sizes in diameter (ISO 50, 90, 110) were used for bending tests. Conventional prefabricated titanium posts of the same sizes served as control groups. The 0.2% yield strengths (R(0.2)) were tested on a universal testing machine. As zirconia posts fractured before they were yielded 0.2%, the fracture strength instead of the yield strength was recorded for these posts. One-way and two-way anova and Bonferroni-Dunn's multiple comparison tests were performed for the statistical analysis. The mean 0.2% yield strengths of the posts were 27 +/- 1 N for glass fibre-reinforced posts and 58 +/- 4 N for zirconia posts of ISO 50 (control group: 54 +/- 3 N). For ISO 90 yield strengths of 52 +/- 4 N for glass fibre-reinforced posts, 117 +/- 20 N for zirconia posts and 117 +/- 11 N for the control group were obtained. For ISO 110 mean yield strengths amounted to 73 +/- 5 N for glass fibre-reinforced posts, 166 +/- 23 N for zirconia posts and 141 +/- 12 N for the control group. Significantly higher yield strength was recorded for the zirconia and the titanium posts compared with the glass fibre-reinforced posts for the tested ISO sizes.

Dental Materials↗