The incidence of multiple drug resistance among enteric pathogens isolated in Iran.
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The results of the study of 65 Brucella strains isolated from myomorphous rodents in the Northern Caucasus are presented. The study was made with the aim of finding out additional characteristics for the identification of these strains. Using the main tests recommended by the FAO/WHO Subcommittee on the Taxonomy of Brucella, as well as some additional tests, we have revealed that the strains under study are very similar to B. suis. At the same time their capacity for agglutination with anti-melitensis monospecific serum, their high sensitivity to pyronin B, safranine T and gentian violet, their low urease activity and their oxidizing activity in respect to L-alanine, L-asparagine, L-glutamic acid, L-arginine, DL-ornithine, DL-citrullin and L-livin make it possible to consider them the fifth independent biotype of B. suis.
Bacteriological evaluation was made on cefotiam (CTM), a new broad-spectrum cephalosporin antibiotic, and the following results wer obtained. 1) CTM has shown very potent antibacterial activities against Staphylococcus aureus, Escherichia coli, Edwardsiella tarda, Citrobacter intermedius, Salmonella, Klebsiella, Proteus mirabilis, Proteus rettgeri, Proteus inconstans, Yersinia enterocolitica, Aeromonas hydrophila, Plesiomonas shigelloides and Pseudomonas putrefaciens. 2) Streptococcus faecalis, Enterobacter and Proteus morganii isolated from urine, Serratia and glucose non fermentative Gram-negative bacilli except Pseudomonas putrefaciens, were almost insusceptible to cefotiam.
The antibiotic susceptibility patterns of bacteria associated with subclinical mastitis in Bloemfontein fresh milk dairy herds were determined. A total of 141 bacterial strains tested, consisted of Staphylococcus aureus (93 strains), coagulase negative staphylococci (17), streptococci (12), Corynebacterium bovis (8), Pseudomonas aeruginosa (7) and enterobacteria (4). Antibiotic susceptibility was determined qualitatively using the Kirby-Bauer disc diffusion method and quantitatively by determining the minimal inhibitory concentrations using the agar dilution method. The utilization of commercial intramammary antibiotic preparations on the dairy farms is also discussed. The Gram-positive bacteria were generally not exceptionally resistant to the antibiotics tested. S. aureus susceptibility figures for penicillin G were 66% and for methicillin (or cloxacillin) 100%. The coagulase negative staphylocci in contrast were relatively more resistant than the coagulase positive staphylococci. The enterobacteria and particularly the P. aeruginosa strains, were extremely resistant to all antibiotics tested. In the latter case even carbenicillin and gentamicin susceptibility figures were low. A general mastitis control programme is discussed.
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1. The increased microbiological control of the total work process at maternity wards enables the timely notice of the contamination location. 2. A direct result of such control is an improvement of hygienic-technological procedures, disinfection and sterilisation at maternity wards. 3. A favourable epidemiological situation was registered during a two-year period following the epidemic (1986-1987) caused by the Coxsackie B virus with respect to a two-year investigation period preceding the epidemic (1984-1985). 4. During the two-year investigation period preceding the epimic 1,942 microbiological controls were performed at the Ginealogical-Obstetrics Clinic in Sarajevo. During the same period following the epidemic 3.321 microbiological controls were performed which is an increase of 1.7 times. 5. Increased number of controls influenced the improvement of the hygienic regime, and the basic parameters were: decreased prevalence of dangerous bacteria species, increased number of sterile swabs and a decreased number of pathogenous isolates. 6. The evaluation of hygiene and epidemiological risk of the medical equipment based on isolated pathogenous carriers prior and after the epidemic shows a significant difference (P less than 0.01). 7. There is a significant statistical difference (P less than 0.01) concerning the hygienic conditions of working clothes with respect to isolated pathogenous carriers before and after the epidemic. 8. Increased microbiological supervision resulted in the elimination of risks associated with working and floor surfaces since findings during the 1986-1987. period in comparison with the 1984-1985. period show significant statistical results with respect to pathogenous isolates (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
INTRODUCTION: Urinary tract infection (UTI) is the most common nosocomial infection among hospitalised patients. Area-specific monitoring studies aimed to gain knowledge about the type of pathogens responsible for UTIs and their resistance patterns may help the clinician to choose the correct empirical treatment. Recent reports have shown increasing resistance to commonly-used antibiotics. We aimed to study the antibiotic resistance pattern of the urinary pathogens isolated from hospitalised patients. METHODS: Three urine samples were collected by the mid-stream "clean catch" method from 1,680 clinically-suspected cases of urinary tract infections from inpatients of various clinical departments during one year. The samples were tested microbiologically by standard procedures. Antibiotic susceptibility of the isolated pathogens was tested for commonly-used antibiotics by Kirby-Bauer technique according to NCCLS guidelines. RESULTS: Significant bacteriuria was present in 71.7 percent of the samples, 17 percent were sterile, 4.8 percent showed insignificant bacteriuria, and 6.5 percent non-pathogenic bacteriuria. The most common pathogens isolated were Escherichia coli (59.4 percent), Klebsiella spp (15.7 percent) and Enterococcus faecalis (8.1 percent). The mean susceptibility was high for amikacin (87.2 percent), ciprofloxacin (74.8 percent), ceftazidime (71.5 percent) and gentamicin (70.4 percent) but low for nitrofurantoin (35 percent), cephalexin (49.7 percent) and ampicillin (50.5 percent). Escherichia coli was found to be most susceptible to amikacin (98 percent) followed by gentamicin (87.9 percent), ceftazidime (80.8 percent), norfloxacin (78.4 percent) and cotrimoxazole (77.9 percent). CONCLUSION: A high isolation rate of pathogens from urine samples of clinically-suspected UTI shows a good correlation between clinical findings and microbiological methods. The antibiotics commonly used in UTIs are less effective. Since the present study was a cross-sectional study, regular monitoring is required to establish reliable information about resistance pattern of urinary pathogens for optimal empirical therapy of patients with nosocomial UTIs.