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At least 217 records · Page 12Linked to original sources
PubMed1993

Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and serotype 0:2 strains of Vibrio anguillarum.

Polyclonal rabbit antisera raised against Vibrio ordalii and serotype 02 strains of Vibrio anguillarum showed extensive cross-reactivity with lipopolysaccharide from these bacterial pathogens of fish when tested in western immunoblot analysis. Results with absorbed polyclonal antisera indicated that lipopolysaccharide molecules from these strains had both common and strain-specific antigenic determinants, which allowed the antisera to be used to differentiate between V. ordalii and serotype 02 strains of V. anguillarum. Unlike rabbits, the immune response in rainbow trout to serotype 02 common antigenic epitopes was dependent on the source of the immunizing lipopolysaccharide antigens. Serum from fish immunized with V. ordalii antigens reacted more extensively with serotype 02 common antigens. In contrast, fish anti-V. anguillarum 02 serum did not interact with O antigens from the V. ordalii strains. Lipopolysaccharide from V. anguillarum serotype 02 and 02a strains showed identical antibody binding properties when interacted with rabbit or fish antiserum to either V. anguillarum 02 or V. ordalii. Lipopolysaccharide from V. anguillarum 02b strains did not interact with the tested rabbit or fish polyclonal sera. The results from this study suggest that fish and rabbits recognise different antigenic determinants in lipopolysaccharide from V. ordalii and serotype 02 V. anguillarum strains; that V. ordalli and serotype 02 strains of V. anguillarum should be regarded as distinct serotype 02 subgroups based on the strain-specific antigenic determinants; and finally that the serological classification of V. anguillarum serotype 02b strains should be reexamined.

Animals↗
PubMed1999

Additional O antigens of Vibrio fluvialis and Vibrio furnissii.

A total of 297 strains of Vibrio fluvialis and Vibrio furrnissii, which were collected from various countries for the past 15-year period of 1984-1998, were serogrouped. Of those examined, 239 strains of V. fluvialis and V. furnissii were classified into 29 known O serogroups; 9 strains were found to belong to R-form cultures, and the rest of the 49 strains could not be serogrouped. Of those serologically untypable strains, 26 novel O serogroups (O36 to O61) were established and added to our reference of the V. fluvialis and V. furnissii antigenic scheme. As all antisera against the O reference strains of the organisms contained some amount of antibody to the rough (R) antigen, all diagnostic O antisera were absorbed with the reference rough strain, V. fluvialis GF25.

Agglutination Tests↗
PubMed1980

[Transposon-induced genetic rearrangements in the chromosome of Vibrio El Tor and their application to the construction of the first genetic map of a cholera vibrio].

The specificity of integration of chloramphenicol resistance transposon (Tn9) into Vibrio El Tor chromosome was studied. As shown by the isolation of auxotrophs offerring various nitritional requirements, Tn9 displayed fairly low specificity in its interaction with the chromosome. When inserted into cholera vibrio chromosome, Tn9 induced secondary rearrangements of genetic material. Nutritional requirements were identified for the polyauxotrophic clones which resulted from the mutations in chromosome region adjacent to the insertion sites of Tn9 and Tn10. Based on these observations a preliminary map was constructed for certain regions of V. eltor chromosome.

Chromosome Mapping↗
PubMed1978

[Study of Vibrio cholerae fractions. II. A comparison of the immunochemical and biological properties of preparations of alkaline extracts (vibrionin) and the endotoxin of Vibrio cholerae].

Comparative study of immuno-chemical and biological properties of vibrionin and cholera vibrio endotoxin showed vibrionin to contain up to 73.5% of protein, whereas endotoxin preparations contained only 25% of protein. Use of disc electrophoresis in polyacrylamide gel permitted to determine the relative electrophoretic mobility of the protein component bands of both preparations. Residual quantities of O-antigenic cholera vibrio determinants were revealed serologically in the vibrionin preparations. Study of biological properties of vibrionin (cholera allergen) demonstrated a possibility of using vibrionin in the capacity of a new bacterial allergen.

Animals↗
PubMed1975

[Isolation under the influence of tetracycline of L-forms of Menchikov's vibrio and the NAG vibrio and their biological properties].

Stable L-forms were for the first time obtained under the effect of tetracycline on the Mec thnikov vibrio and NAG vibrio. The process of conversion of the L-forms under the effect of tetracycline was the same as under the action of penicilline. Three types of the L-forms were obtained: stable tetracycline-resistant L-forms, stable tetracyclin-superresistant and tetracycline-dependent L-forms. Under the reversion of the unstable L-forms strains with a typical complex of biological properties and atypical revertants were obtained.

Bacteriological Techniques↗
PubMed1977

[Cholera vibrio fractions. I. Isolation of alkaline extracts of cholera vibrios and a study of their biological properties].

The authors suggest a well-reproducible method of preparation of a biologically-active fraction of an alkaline extract of cholera vibrio from the strain 569B of Inaba serological type consisting in a preliminary triple removal of endotoxin (O-antigen) of the causative agent from the microbial cell suspension with trichloracetic acid with the subsequent extraction of the microbial mass and neutralization of the extract obtained capable of effective detection of the state of specific sensitization of the organism to cholera vibrio. Preparations of the alkaline extracts (cholera allergen) obtained were standard by chemical composition, they were characterized by a high protein content (up to 77%) and were practically nontoxic. These preparations can be used as a bacterial allergen for the experimental investigations.

Allergens↗
PubMed1986

The sodium cycle. III. Vibrio alginolyticus resembles Vibrio cholerae and some other vibriones by flagellar motor and ribosomal 5S-RNA structures.

An electron microscopic study of the basal bodies of the Vibrio albinolyticus flagellum revealed a four-disc structure. The diameters of the two discs localized closer to the cytoplasmic membrane proved to be about 2-fold shorter than those of the two others. In this respect the basal body of V. alginolyticus resembles very much that of V. cholerae described by Ferris and co-workers. The sequence of the V. alginolyticus ribosomal 5S-RNA showed that it is similar to those of V. cholerae, V. harveyi and some other vibriones. On the basis of the 5S-RNA sequences, a dendrogram of prokaryotes is presented. It confirmed the suggestion that V. alginolyticus is a typical representative of Vibrionaceae rather than a 'monster' greatly differing from other vibriones. Possible evolutionary relation of various bacterial species possessing the primary Na+ pumps is discussed.

Base Sequence↗
PubMed1994

Genetic variation in the humoral immune response against Vibrio salmonicida and in antibody titre against Vibrio anguillarum and total IgM in Atlantic salmon (Salmo salar).

Total IgM level and antibody titre to Vibrio anguillarum O-antigen after bath-vaccination, and specific antibody response to V. salmonicida O-antigen at three different samplings were analysed in family material of Atlantic salmon (Salmo salar), consisting of 791 fish belonging to 34 maternal full-sib groups within 12 paternal half-sib groups. The fish were immunized twice, and blood samples collected three times. After the third blood sampling, the fish were challenged with V. anguillarum. Medium to low genetic variation was recorded in total IgM and in the antibody titres against V. anguillarum O-antigen and V. salmonicida O-antigen, with heritability estimates of 0.12, 0.18 and from 0.03 to 0.12, respectively. Moderate to high genetic and phenotypic correlations were found between the V. salmonicida O-antigen titres at different samplings. Genetic and phenotypic correlations between the initial titres were moderate to low. The effect of different immune traits, including Aeromonas salmonicida A-layer titres (previously described), on the ability to survive the challenge was examined. The likelihood of surviving the challenge was affected positively by the A. salmonicida A-layer titre at the second sampling, and almost significantly affected by the initial V. anguillarum O-antigen titre. Production traits, such as mean slaughter weight and mean proportion of survivors in a corresponding full-sib material, were obtained in the sea-rearing period. No significant full-sib correlation between immune parameters and production traits was detected.

Animals↗
PubMed1991

Purification and characterization of a hemolysin of Vibrio mimicus that relates to the thermostable direct hemolysin of Vibrio parahaemolyticus.

A hemolysin (designated Vm-rTDH) from Vibrio mimicus (AQ0915-E13) was purified by ammonium sulfate fractionation and successive column chromatography with DEAE-Sephadex A-25, hydroxyapatite, Mono Q, Superose 12 and Phenyl-Superose. The Mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. The isoelectric point of Vm-rTDH was approximately pH 4.9. The hemolytic activity of Vm-rTDH was stable upon heating at 100 degrees C for 10 min, similar to that of the thermostable direct hemolysin (Vp-TDH) of V. parahaemolyticus. Vm-rTDH also showed lytic activities similar to those of Vp-TDH. Immunological cross-reactivity between Vp-TDH and Vm-rTDH was demonstrated by the Ouchterlony double-diffusion test. Thus we conclude that V. mimicus produces a newly discovered type of hemolysin (Vm-rTDH) which is similar to Vp-TDH.

Bacterial Proteins↗
PubMed2003

A simple and convenient microtiter plate assay for the detection of bactericidal antibodies to Vibrio cholerae O1 and Vibrio cholerae O139.

It is believed that the correlate of protection for cholera can be determined by the serum vibriocidal assay. The currently available vibriocidal assays, based on the conventional agar plating technique, are labor intensive. We developed a simple and convenient microtiter plate assay for the detection of vibriocidal antibodies that is equally as efficient for Vibrio cholerae O1 and for V. cholerae O139. The addition of succinate and neotetrazolium made it possible to measure the growth of surviving bacterial target cells by monitoring a color change. We evaluated assay parameters (target strains, growth of target cells, complement source and concentration) that may affect the reproducibility of the method for V. cholerae O139. The results obtained with the microtiter plate assay were uniformly similar to those obtained with the conventional agar plating assay, when testing both the Inaba and Ogawa serotypes of V. cholerae O1. The microtiter plate assay was also convenient for measuring the activity of animal sera and mouse monoclonal antibodies.

Animals↗
PubMed1999

Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel. Israel Vibrio Study Group.

BACKGROUND: Vibrio vulnificus is a gram-negative bacterium that causes septicaemia and wound infection. Cases occur sporadically, and no previous outbreaks due to a common source or a clonal strain have been reported. In the summer and autumn of 1996 and 1997, an outbreak of invasive V. vulnificus infection occurred in Israel in people who had recently handled fresh, whole fish purchased from artificial fish-ponds. METHODS: We reviewed clinical and epidemiological information, and undertook an environmental investigation to assess disease characteristics, modes of transmission, phenotypic characteristics of the bacterium, and fish-marketing policy. The clonal nature of 19 isolates was studied by biotyping, pulsed-field gel electrophoresis, and restriction-fragment length polymorphism (RFLP) analysis of a PCR fragment. FINDINGS: During 1996-97, 62 cases of wound infection and bacteraemia occurred. 57 patients developed cellulitis, four had necrotising fasciitis, and one developed osteomyelitis. In all cases, the fish were cultivated in inland fish-ponds. In the summer of 1996, fish-pond managers initiated a new marketing policy, in which fish were sold alive instead of being packed in ice. Phenotypically, the isolates had five atypical biochemical test results. The isolates were non-typeable by pulsed-field gel electrophoresis, and all had the same PCR-RFLP pattern which had not been seen previously. INTERPRETATION: The cause of the outbreak was a new strain of V. vulnificus, classified as biogroup 3. A new fish-marketing policy that began in 1996 may have exposed susceptible people to the organism.

Adolescent↗
PubMed1998

Genetic organization of the regions associated with surface polysaccharide synthesis in Vibrio cholerae O1, O139 and Vibrio anguillarum O1 and O2: a review.

Vibrio cholerae and V. anguillarum are recognized as aquatic-borne human and fish pathogens, respectively. Based upon analyses of several genes and the presence of novel genetic elements it seems that these two species are very closely related. Studies in this laboratory have identified an association of IS1358 with rfb and capsule loci in these two species. The most recent findings suggest that IS1358 is associated with the rfb region in V. cholerae O1 and O139 and in V. anguillarum O1 and O2. In addition, the rfb region in both V. cholerae serogroups and in V. anguillarum O1 is limited at one end by gmhD. These features make it feasible to envisage a mechanism by which the evolution of new rfb genes is taking place involving IS1358 and the region around gmhD. Furthermore, it is possible to envisage that there is or has been an exchange of genetic material between these species leading to new rfb/capsule regions. This review examines the genetics and biosynthesis of the O-antigen and capsule of V. cholerae O1 and O139, as well as the V. anguillarum serogroup O1 and the role of IS1358. Throughout this review we have used the new nomenclature for rfb genes proposed by.

Bacterial Proteins↗
PubMed2002

Vibrio vulnificus cytolysin induces hyperadhesiveness of pulmonary endothelial cells for neutrophils through endothelial P-selectin: a mechanism for pulmonary damage by Vibrio vulnificus cytolysin.

Vibrio vulnificus cytolysin forms transmembrane pores that are permeable to calcium ions in pulmonary endothelial cells, and has been suggested as an important virulence factor that sequestrate neutrophils primarily in the lung. To elucidate the mechanism we investigated whether the cytolysin affect the expression of endothelial P-selectin and adhesiveness of pulmonary endothelial cells for neutrophils. The cytolysin increased the adhesiveness of CPAE cell, a pulmonary endothelial cell line, for neutrophils in a concentration- and time-dependent manner. The increase of adhesiveness occurred within several minutes after the cytolysin exposure, persisted up to 90 min, and was not affected by cycloheximide. Furthermore, flow cytometric analyses showed that cytolysin enhanced the level of P-selectin on CPAE cell surface. Therefore, these results suggest that the cytolysin-induced hyperadhesiveness of pulmonary endothelial cells for neutrophils is mediated by the mobilization of endothelial P-selectin to the cell surface.

Animals↗
PubMed1988

Immunobiological relationships between Vibrio fluvialis and Vibrio cholerae enterotoxins.

A total of 26 strains of Vibrio fluvialis was included in this study, which were isolated from patients with diarrhoea and other sources. The GM1 enzyme linked immunosorbent assays performed with the culture filtrates of V. fluvialis yielded negative results, indicating that their receptor site is different from that of the known labile toxin. The cholera antitoxin failed to neutralize the skin permeability factor activities of all the V. fluvialis culture filtrates and none of the concentrated culture filtrates gave any precipitin band, when tested against the cholera antitoxin in Ouchterlony's gel diffusion test. These observations suggest that the toxin of V. fluvialis differs from the known cholera toxin in receptor site, mode of action and antigenicity.

Cholera Toxin↗
PubMed1998

The role of Gulf Coast oysters harvested in warmer months in Vibrio vulnificus infections in the United States, 1988-1996. Vibrio Working Group.

Vibrio vulnificus infections are highly lethal and associated with consumption of raw shellfish and exposure of wounds to seawater. V. vulnificus infections were reported to the Centers for Disease Control and Prevention from 23 states. For primary septicemia infections, oyster trace-backs were performed and water temperature data obtained at harvesting sites. Between 1988 and 1996, 422 infections were reported; 45% were wound infections, 43% primary septicemia, 5% gastroenteritis, and 7% from undetermined exposure. Eighty-six percent of patients were male, and 96% with primary septicemia consumed raw oysters. Sixty-one percent with primary septicemia died; underlying liver disease was associated with fatal outcome. All trace-backs with complete information implicated oysters harvested in the Gulf of Mexico; 89% were harvested in water >22 degrees C, the mean annual temperature at the harvesting sites (P < .0001). Control measures should focus on the increased risk from oysters harvested from the Gulf of Mexico during warm months as well as education about host susceptibility factors.

Adolescent↗
PubMed1981

A comparative study of the sugar composition of O-antigenic lipopolysaccharides isolated from Vibrio alginolyticus and Vibrio parahaemolyticus.

A comparative study of the sugar composition of O-antigenic lipopolysaccharides (LPS) isolated from Vibrio alginolyticus and those from V. parahaemolyticus was carried out. 3-Deoxy-D-mannooctulosonic acid, 2-keto-3-deoxy octonate (KDO), a regular sugar constituent of gram-negative bacterial LPS, was totally absent from LPS of all V. alginolyticus strains examined as it was from those of V. parahaemolyticus. Furthermore, a KDO-like thiobarbituric acid test-positive substance, identical with that of either V. parahaemolyticus 07 or 012, was also found in LPS from three strains, 505-78, 905-78, and 1013-79 (designated tentatively as group I), out of the five strains of V. alginolyticus tested. LPS from the members of group I contained, as component sugars, glucose, galactose, L-glycero-D-manno-heptose, glucosamine, galactosamine, the KDO-like substance, and an unidentified amino sugar P1. Thus, LPS of the members of group I possessed a similar sugar composition which is similar to that of LPS from either V. parahaemolyticus 97 or 012. LPS of strain 1027-79, one of the other two strains (designated tentatively as group II), contained as component sugars, glucose, L-glycero-D-mannoheptose, glucosamine, galactosamine, galactosamine, and the other unidentified amino sugar P2, while LPS of strain 53-79, the other member of group II, contained galactose as an additional component. The results indicate that LPS of strain 1027-79 has a sugar composition similar to that of V. parahaemolyticus 09 LPS.

Amino Sugars↗
PubMed1993

Release of the outer membrane vesicles from Vibrio cholerae and Vibrio parahaemolyticus.

We found numerous small vesicles released from the cell by thin sectioning of the plate culture of Vibrio cholerae and V. parahaemolyticus fixed with the freeze-substitution technique. From the broth media of exponentially growing bacteria we could collect the vesicles by the centrifugation but not enough without fixation. The vesicles are encompassed with a membrane structure similar to the outer membrane of these bacteria. The anti-O (Inaba) serum reacted with the surface of the vesicles and the inside of the vesicle are generally filled with an electron-dense mass.

Bacterial Proteins↗
PubMed2006

Presence of a lethal protease in the extracellular products of Vibrio splendidus-Vibrio lentus related strains.

The presence of a lethal extracellular 39-kDa protease, a virulence determinant of a Listonella pelagia strain which produces vibriosis in turbot, was determined in the extracellular products (ECP) of 33 Vibrionaceae strains. Both immunological and enzymatic techniques distinguished this specific protease from other Vibrionaceae proteins. It was detected in 15% (5/33) of the ECPs assayed belonging to strains of the Vibrio splendidus-V. lentus related group isolated in Galician aquaculture systems (NW Spain). As these strains were associated with diseased octopus and cultured turbot, were able to colonize the internal organs of fish and produced a lethal ECP for fish, they are a potential risk for the health of reared aquatic organisms.

Animals↗