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At least 217 records · Page 12Linked to original sources
PubMed1977

Biologic laws governing functions of muscles that move the mandible. Part IV. Degree of jaw separation and potential for maximum jaw separation.

Accurate diagnosis and treatment of the gnathostomatic system require an understanding of both its biomechanics and its physiology. Clinically, the treatment consists of modifying the existing occlusal program to relieve stress from distressed tissues. The objective of treatment is to effect a neuromuscular release of the mandible. This may be accomplished by (1) equilibration of the natural occlusion, (2) orthodontics, (3) restorative procedures, or (4) surgery. Most frequently, however, the treatment entails some combination of these procedures. The criteria for success of treatment are not determined by the method or technique employed but by the neuromuscular response that the treatment produces. The purpose of this article is not to suggest which occlusal scheme produces the most favorable muscle response in the gnathostomatic tissues. Neither is an objective of this article to teach the mandible-manipulation techniques that are needed to identify occlusal irritants, perform occlusal equilibrations, or obtain accurate centric relation recores. The objective of this article is to emphasize that there are definite principles by which the muscles respond to occlusal contacts. Occlusal contacts can excite bruxism and the sequelae to bruxism. Knowledge of the laws which govern functions of the muscles that move the mandible enables the dentist to acquire the mandibular-manipulation skills that are necessary for the effective diagnosis and treatment of occlusal conditions.

Biology↗
PubMed2005

Characterization of a gel-separated unknown glycoprotein by liquid chromatography/multistage tandem mass spectrometry: analysis of rat brain Thy-1 separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

We developed an efficient and convenient strategy for protein identification and glycosylation analysis of a small amount of unknown glycoprotein in a biological sample. The procedure involves isolation of proteins by electrophoresis and mass spectrometric peptide/glycopeptide mapping by LC/ion trap mass spectrometer. For the complete glycosylation analysis, proteins were extracted in intact form from the gel, and proteinase-digested glycoproteins were then subjected to LC/multistage tandem MS (MSn) incorporating a full mass scan, in-source collision-induced dissociation (CID), and data-dependent MSn. The glycopeptides were localized in the peptide/glycopeptide map by using oxonium ions such as HexNAc+ and NeuAc+, generated by in-source CID, and neutral loss by CID-MS/MS. We conducted the search analysis for the glycopeptide identification using search parameters containing a possible glycosylation at the Asn residue with N-acetylglucosamine (203 Da). We were able to identify the glycopeptides resulting from predictable digestion with proteinase. The glycopeptides caused by irregular cleavages were not identified by the database search analysis, but their elution positions were localized using oxonium ions produced by in-source CID, and neutral loss by the data-dependent MSn. Then, all glycopeptides could be identified based on the product ion spectra which were sorted from data-dependent CID-MSn spectra acquired around localized positions. Using this strategy, we successfully elucidated site-specific glycosylation of Thy-1, glycosylphosphatidylinositol (GPI)-anchored proteins glycosylated at Asn23, 74, and 98, and at Cys111. High-mannose-type, complex-type, and hybrid-type oligosaccharides were all found to be attached to Asn23, 74 and 98, and four GPI structures could be characterized. Our method is simple, rapid and useful for the characterization of unknown glycoproteins in a complex mixture of proteins.

Amino Acid Sequence↗
PubMed2001

Liquid exclusion adsorption chromatography, a new technique for isocratic separation of nonionic surfactants. III. Two-dimensional separation of fatty alcohol ethoxylates.

A quantitatively accurate mapping of lower fatty alcohol ethoxylates can be achieved using a combination of liquid chromatography under critical conditions as the first dimension and liquid exclusion-adsorption chromatography as the second dimension. With coupled density and refractive index detection in both dimensions, the contribution of preferential solvation can also be estimated. In most cases, however, the use of refractive index detection alone also yields satisfactory results.

Adsorption↗
PubMed1991

Use of chromatofocusing for separation of beta-lactamases. IX. Analytical chromatofocusing for the separation of a chromosomal cephalosporinase from Proteus vulgaris 1028.

Simultaneous purification and isoelectric point (pI) determination was carried out at analytical scale of the chromosomal cephalosporinase from the Proteus vulgaris 1028 strain. Comparison of the enzyme to the purification results with m-aminophenylboronic acid-agarose affinity chromatography with sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that minute amounts of accompanying proteins having identical pI values but different molecular masses were found in the chromatofocused preparation. The molecular mass of the enzyme was 24,000 dalton. The pI was found to be 8.3.

Cephalosporinase↗