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At least 217 records · Page 12Linked to original sources
PubMed1980

Kinetics of classes and sub-classes of total immunoglobulins and specific antibodies to Schistosoma mansoni during murine infection.

During the course of Schistosoma mansoni murine infection there is a dramatic increase of some immunoglobulins and S. mansoni-specific antibodies. The most substantial response is initiated after 40 days of infection and results in a prolonged increase of total IgG1, IgM and IgA. The maximum increase is respectively 26, 14 and 3-fold the basic immunoglobulin level in control mice. Some anti-S. mansoni classes and sub-classes were studied by an original radio-immunoadsorbent test. Anti-S. mansoni IgG1 and IgM antibodies appear and increase at the same time as that of total IgG1 and IgM. Anti-S. mansoni IgA antibodies appear later (80th day) and correspond to a second peak of total IgA.

Animals↗
PubMed1983

A comparison of the Phadebas and Phadezym IgE paper immunosorbent test kits.

The Phadebas (isotype label) and Phadezym (enzyme label) paper immunosorbent test kits have been compared. Precision and ease of operation are shown to be comparable for both kits, and the advantages and disadvantages of each are similar. The enzymatic labelled kit seems to provide a suitable alternative for laboratories wishing to measure IgE but who do not have isotope-counting facilities available.

Humans↗
PubMed1981

The occurrence of immunoglobulin E in Human seminal plasma.

The presence of IgE in human seminal plasma has been explored using two different radioimmunoassay methods. Samples were obtained from 84 patients under investigation for involuntary infertility. Low levels were recorded in seminal plasma (less than 0.05-805 kIU/1) together with a wide scatter of serum values (less than 0.5-956 kIU/1). The incidence of detectable seminal plasma IgE was related to high serum IgE values (P less than 0.001; chi 2-test). Among men reporting allergy problems and who has serum IgE levels above 50 kIU/1 detectable semen IgE was seen in 15 out of 18 patients. Sperm agglutination was observed in 18 men of whom 11 had detectable seminal plasma IgE. The question of whether or not seminal plasma IgE is a plasma transudate or is locally produced as well as its possible function in seminal plasma is discussed.

Female↗
PubMed1990

Problems in defining normal limits for serum IgE.

Recommended "normal limits" for serum IgE generally assume that a single upper limit of normal can be applied to all adults. The present article describes the distribution of IgE levels in 2657 subjects in a general population sample in Tucson, Ariz. Limits of IgE defining the lower 5%, 10%, 25%, 50%, 75%, 90%, and 95% in 1569 subjects with negative allergy skin tests and without current asthma, considered a reference group, are provided by age and sex. These cutoffs are then used to compare groups with asthma and with positive allergy skin tests with the reference population. Distributions of IgE levels in these groups are vastly different, but defining an upper "limit of normal" for serum IgE is of doubtful clinical value because there is no single level of IgE that distinguishes different groups with any precision. The spread of IgE values is extremely wide in subjects with and without known allergic diseases.

Adolescent↗
PubMed1985

[Study on the genesis of atopic dermatitis--atopic dermatitis and IgE].

For the purpose of investigating how IgE is correlated in the genesis of Atopic dermatitis (abbreviated as AD) micronizing method of RIST (necessary serum 10 microliter) was utilized. Serum IgE obtained from 369 cases AD, age ranged from 1 month old to 52 years old were studied. In 78 cases, IgE of the objects' parents were also studied as well. Serum IgE of the healthy control, age ranging from 3 days old to 49 years old, were studied and made comparison with those of the objects. Analysis was made in accordance with the factors like age, complication, disease type and prognosis. RAST (house dust, mite, egg, milk) and SKIN TEST (Scratch test) were carried out in order to observe the correlation between serum IgE and Specific IgE or to see the possible induction of eczematous lesion by specific IgE. The following were obtained as conclusion. Serum IgE of AD will show high level in significant manner in comparison with the healthy control from the time of infantile period. Low IgE group will show high rate of improvement after 3 years period of time. High IgE of parents will more or less affect serum IgE of their child or children. Correlation between serum IgE and the severity of AD is not noted in the infantile period but significant in the adult period. In the Scratch test immediate type of reaction could not induce eczematous lesion of AD. Induction of eczematoid reaction in AD was noted with high incidence following the Scratch test of Candida antigen. From the above it is assumed that in AD, although serum IgE level is high, induction of eczematous lesion could not be made by skin invasion of specific IgE antigen to the skin, pruritus by immediate type of reaction following release of chemical mediator will bring the tearing off the skin by scratch and invasion to the epidermis of inflammation inducing materials like human dander, sweat, bacteria or mycological agents eczematous lesion will start to take place.

Adolescent↗
PubMed1978

[Serum IgE in skin diseases of children (author's transl)].

Using the paper-radioimmunosorbens test (PRIST) normal values were obtained from 200 non-allergic children. The IgE level was aged-dependent, in part with significant differences between various age groups. These normal values were compared with those in children with seborrhoeic dermitis, constitutional neurodermatitis, parasitoses, urticaria, Quincke oedema, Schönlein-Henoch purpura, pityriasis rosea, multiform exudative erythema, erythema nodosum and infantile papular acrodermatitis. Half-life of IgE in neonates was 16.2 hours.

Acrodermatitis↗
PubMed1977

Measurement of IgE on human basophils: relation to serum IgE and anti-IgE-induced histamine release.

The number of IgE molecules bound to human basophils was calculated from direct measurements of the IgE dissociated after exposing leukocytes to pH 3.7 acetate buffer in the cold. In 18 donors studied, cell-bound IgE ranged from 4000 to 500,000 molecules/basophil and correlated with the serum IgE concentration (r = 0.89, p less than 0.001) which ranged from 5 to 3,000 ng/ml. Sensitivity of these cells to anti-IgE was tested to explore the relationship between cell-bound IgE and the concentration of anti-IgE required for histamine release. Cells from some nonatopic donors (4000 to 100,000 IgE molecules/basophil) were as sensitive as cells from allergic donors (100,00 to 500,000 IgE molecules/basophil). Moreover, cells from donors having approximately the same cell-bound IgE concentration varied widely in their sensitivity to anti-IgE. We conclude that an intrinsic property of human basophils ("releasability") is an important parameter in determing mediator release.

Acetates↗
PubMed1985

Measurement of total IgE antibody levels in lacrimal fluid of patients suffering from atopic and non-atopic eye disorders. Evidence for local IgE production in atopic eye disorders?

Total IgE levels in lacrimal fluid of patients suffering from different eye disorders were quantitatively measured by a modification of the paper radio immuno sorbent test (PRIST). The geometric mean values for patients with atopic conjunctivitis, patients with keratoconjunctivitis vernalis, and patients with asthma without conjunctivitis differed significantly from those for control persons and those for patients without atopic conjunctivitis. Besides lacrimal fluid IgE levels, serum IgE levels as well as lacrimal fluid and serum albumin levels were measured. From these values the local IgE production was calculated. Although there seemed a good correlation between the level of lacrimal fluid IgE and the amount of local IgE production, the results suggest that local IgE production in lacrimal fluid is not restricted to patients with atopic eye disorders only.

Adolescent↗
PubMed1991

Human IgE-binding synthetic peptides of bovine beta-lactoglobulin and alpha-lactalbumin. In vitro cross-reactivity of the allergens.

The allergenicity of cow's milk whey proteins, purified by high performance liquid chromatography (HPLC), was examined by the radio-allergosorbent test (RAST) against the sera of children immediately hypersensitive to milk. beta-lactoglobulin and alpha-lactalbumin bound specific IgE in the sera of 63% and 75% of these patients respectively. These allergens were tested for cross reactivity with each other by RAST inhibition. Both inhibited the binding of IgE, in the sera of allergic patients, to the other protein. Two possible determinant peptides, one from beta-lactoglobulin and one from alpha-lactalbumin, were selected by computer prediction of antigenic sites and synthesized by the fluorenylmethoxycarbonyl (FMOC)-polyamide method. The peptides were adsorbed to nitrocellulose discs and used in further RAST studies with sera from the allergic children. Both peptides bound specific IgE in the RAST assay.

Allergens↗
PubMedDate not supplied

[2 variants of the radioimmunochemical method of determination of LH-releasing factor (LH-RF). Distribution of LH-RF in various hypothalamic structures].

The authors elaborated two variants of the radioimmunochemical method of determination of the LH-releasing factor (LH-RF). Diluted antisera (AS) obtained by the immunization of rabbits with the LH-RF-ovalbumin were used in the first method; AS polymerized with isobutyl ester of chlorcarbonic acid were used in the second one. The minimal amount of the hormone determined in the I variant was 5 pg, and in the 11 one--20 to 30 pg. The method is highly specific, since the antisera gave no cross reaction with a wide set of protein-peptide hormones synthesized with the hypothalamus and the hypophysis. The presence in the samples of tissue components of the hypothalamus and of the cerebral cortex extractable with 2M CH3COOH failed to influence the precision of the test. In examining the LH-RF content in the brain structures there was revealed that in the sexually mature male rats the whole hypothalamus contained an average of 7.5 ng, the arcuate nuclei--1.5 ng and the medial eminence--2.5 ng of LH-RF; the supraoptic nuclei and the cerebral cortex contained no LH-RF determinable by the method described. Two weeks after the castration the mean content of the hormone in the whole hypothalamus decreased to 5.5 ng, in the medial eminence--to 0.6 ng, and showed no significant change in the arcuate nuclei.

Animals↗
PubMed1986

A radiometric immunosorbent assay for the detection of anti-hormone-binding protein antibodies.

A radiometric immunosorbent assay (RISA) for the detection of monoclonal antibodies to hormone-binding proteins has been developed. The assay involves incubating hybridoma supernatants in microtiter wells that have been coated with goat anti-mouse IgG antibodies. Any mouse IgG in the test supernatant is thus specifically retained in the wells. Radioactive ligand-binding protein complexes are then incubated in the wells. The presence of anti-binding protein antibodies in the supernatant is indicated by specific retention of radioactive ligand-binding protein complexes in the wells. Crude antigen preparations, such as tissue homogenates, can be used to detect antibodies. The assay is capable of detecting antibody at concentrations 20 ng/ml (approximately 100 pM IgG). The RISA has been used successfully to screen for monoclonal antibodies to the intracellular receptor for 1,25-dihydroxyvitamin D3 and should be useful for the detection of antibodies to ligand-binding proteins in general.

Animals↗
PubMed1986

Slide immunoenzymatic assay for human IgE (SIA-IgE).

A rapid and inexpensive method is described for determination of human serum IgE using 5-microliter samples. The slide immunoenzymatic assay for IgE (SIA-IgE) is carried out on a glass slide in which up to 24 samples can be tested including controls and standards; up to four of these slides can be read automatically in conventional vertical beam readers. Flat circles on the slide are covered with a layer of biotinylated antibody specific for human IgE (trapping antibody). Five microliters of serum sample is dropped to cover each circle, and the slide is incubated. The circles are washed with water, dried, incubated under 10 microliters of enzyme-labeled antibody to human IgE, then washed again, and covered with 10 microliters of enzyme substrate. The intensity of color generated is measured at the proper wavelength. The method is simple, accurate and non-radioactive and can be completed within 2 h.

Antibodies, Anti-Idiotypic↗
PubMed1980

[Serum IgE level in autosomal dominant and X chromosomal recessive ichthyosis vulgaris].

Thirty-five patients with ichthyosis were classified according to the clinical features, the genetic and histological findings, the association with manifestations of atopy and the result of the skin tests into the following three groups: (1) Autosomal dominant ichthyosis (ADI) with atopy (n = 13), (2) ADI without atopy (n = 17) and (3) X-linked ichthyosis (n = 5). Serum IgE levels were determined in patients and in a normal control group (n = 93) with the Phadebas-IgE-PRIST. The frequency of the association of ADI with atopy was with 44% in accordance with the literature reports. The geometrical means of the IgE values in the ADI-group with atopy were statistically significantly higher than in the ADI group without atopy (p less than 0.05) or in the control group (p less than 0,01). The increase of the IgE-levels in ichthyosis is due to the associated atopy.

Female↗
PubMedDate not supplied

[Simple and rapid heterologous method of radioimmunochemical determination of luteinizing hormone in the blood serum of rats. 2. Specificity of the method].

The Heterologous hard phase radioimmunochemical method (RICM) of luteinizing hormone (LH) determination was tested in a number of experimental models for specificity. The results obtained demonstrated that a possible factor of cross reaction with the thyrotropic hormone (TTH) and the folliculo-stimulating hormone (FSH) was apparently insignificant in determination of LH in rat serum. This conclusion was based on the following. An immunosorbent used in RICM, prepared from the antisera of rabbits immunized with sheep LH, failed to interact with the FSH of rat hypophysis extracts. TTH-releasing hormone failed to alter the level of the immunoreactive LH in rat serum, whereas LH-releasing hormone increased 31-fold the LH concentration in 20 min. Besides, by means of RICM there was recorded a marked increase of the LH in the serum of rats after ovariectomy and a fall of the LH concentration in response to the administration of estradiol together with progesteron to such rats.

Animals↗
PubMed1984

Immunoglobulin E in extracts of feces from children.

Immunoglobulin E (IgE) was demonstrated by a double antibody radioimmunoassay technique (PRIST) in 5 of 17 unconcentrated fecal extracts from children. Four of the PRIST-positive extracts also had measurable levels of IgE determined by an enzyme-linked immunosorbent test (Enzygnost IgE). However, using a competitive antibody radioimmunoassay technique (RIST), IgE was found in all unconcentrated fecal extracts. The RIST-IgE levels of the extracts were higher and did not correlate to IgE measured by the other methods, nor to the IgE in serum from the same children or to the manifestation of allergy. On the other hand, 4 of the 5 children with measurable PRIST-IgE levels, and 3 of the 4 children with detectable Enzygnost IgE concentrations in the extracts, had elevated serum IgE as well as a history of allergy. Gel filtration studies indicated that IgE determined by the PRIST and Enzygnost IgE methods had been degraded to fragments of lower molecular weight than that of albumin. This study also suggests that the IgE in feces measured by the RIST method is overestimated due to the influence from nonspecific substances of high molecular weight.

Child↗
PubMed1984

Serum IgE in primary glomerular diseases.

Serum IgE was measured by RIST test in 181 adult subjects, with log transformation. In 24 controls the geometric mean was 109 IU/ml, with an upper limit (geometric mean +/- 2 SD) of 460 IU/ml. 157 cases of glomerulonephritis (GN) classified according to light and immunofluorescence microscopy were studied. Serum IgE was significantly elevated in 39 lipoid nephrosis patients (p less than 0.001, with 21 levels greater than 460 IU/ml), 21 focal and segmental glomerulosclerosis (p less than 0.05, with 8 levels greater than 460 IU/ml) and 42 membranous GN (p less than 0.01, with 10 levels greater than 460 IU/ml). In 27 membrano-proliferative GN and 28 IgA GN, serum IgE was not different from controls with respectively 4 and 3 levels greater than 460 IU/ml. In addition, the lipoid nephrosis IgE geometric mean is 493 IU/ml, which is keeping with the high incidence of atopic troubles. The significance of raised serum IgE was discussed.

Adolescent↗
PubMed1984

The natural history of mountain cedar pollinosis.

During the winter months, pollen from the mountain cedar (MC) (Juniperus sabinoides) causes severe respiratory tract allergy in central Texas. We have been impressed with the fact that many of our MC-allergic patients had only allergic rhinitis and were only sensitive to MC pollen. We therefore studied 234 unselected MC-allergic patients at the end of the MC season. The main criteria for inclusion into the study was a bona fide history of MC pollinosis confirmed by a positive skin test. All patients completed a detailed history questionnaire, were prick skin tested by using 1:20 w/v, extracts, and had a total IgE determined with commercially available reagents (PRIST). Thirty-four percent of patients were found to be allergic only to MC and 66% were allergic to MC and other aeroallergens. As a group, patients allergic only to MC had significantly lower total IgE levels (84 IU/ml vs. 360, p less than 0.001) (normal less than 180), required much longer exposure intervals to cedar pollen before developing MC pollinosis (14.4 yr vs. 5.69, p less than 0.001), had less of a family history of allergic disease (43% vs. 88%, p less than 0.001), had less of a history of asthma or eczema (11% vs. 39%, p less than 0.001), and developed allergic disease at a later age (39 yr vs. 13, p less than 0.001) when compared to patients with multiple allergies. These results suggest that the MC pollen may be unique in causing allergic rhinitis in patients who have no other sensitivities. A possible explanation may lie in the carbohydrate nature of the main allergen of the MC pollen, which may facilitate allergen transport through the respiratory tract mucosa and subsequent sensitization.

Adult↗
PubMed1982

Basophil sensitisation in idiopathic nephrotic syndrome.

The degranulation of basophils in the presence of a specific allergen (human basophil degranulation test, HBDT) is an index of IgE-dependent cellular response and it was tested in 46 unselected idiopathic nephrotic syndrome (INS) patients with or without atopic manifestations. 28 of the INS patients had "minimal change" nephrotic syndrome (MCNS) and 18 had focal segmental glomerular sclerosis (FSGS). 29 patients with different types of primary glomerulonephritis (GN) and 11 healthy donors acted as controls. The HBDT was positive in 16 (57%) of 28 MCNS and in 14 (77.8%) of 18 FSGS patients. Only 5 (17.2%) of 29 GN and 1 (6%) of 11 blood donors exhibited positive HBDT. House dust was the commonest allergen to which MCNS and FSGS subjects were sensitive, whereas sensitivity to grass pollen, moulds, and animal danders was less common. 8 MCNS and 8 FSGS subjects were sensitive to house dust plus another allergen. 4 MCNS and 1 FSGS patient had fewer than 5/microliters basophils; 4 of these 5 patients were having a relapse of their nephrotic syndrome at the time. The demonstration of enhanced IgE-basophil sensitisation in INS patients suggests than an anaphylactic process plays a part in the pathogenesis of INS.

Adolescent↗