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PubMed1989

The effect of incorporating fat into different components of a meal on gastric emptying and postprandial blood glucose and insulin responses.

1. Three studies were carried out in each of six normal volunteers to investigate how lipid, when given at different stages during the course of a meal, affects gastric emptying and postprandial blood glucose and insulin concentrations. 2. The control meal consisted of 300 ml beef consommé (50 kJ, 12 kcal), followed 20 min later by 300 g mashed potato (908 kJ, 217 kcal). In the two test meals, 60 g margarine were incorporated into either the soup or the mashed potato. 3. The addition of margarine to either component of the meal delayed gastric emptying of the mashed potato (P less than 0.05), but the pattern varied according to the component to which the fat was added. 4. Incorporation of fat into the soup increased the lag phase (P less than 0.05) but did not influence the slope of emptying of the mashed potato, while incorporation of fat into the mashed potato reduced the slope of emptying of the mashed potato (P less than 0.05) but did not influence the lag phase. 5. Addition of fat to either component of the meal reduced postprandial blood glucose (P less than 0.05) and insulin responses, but when the fat was incorporated in the soup, peak glucose and insulin responses were delayed as well (P less than 0.05). 6. The results show that the effect of fat on gastric emptying and absorption of nutrients depends on when, in relation to the other components of the meal, the fat is consumed.

Adult↗
PubMed2002

Dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella Enteritidis colonization.

This study first investigates the effects of mash diet, or mash supplemented with either 2.5% mannose-oligosaccharide (MOS) or palm kernel meal (PKM), on the microflora of the hen caecal contents. Second, it investigates the effect of caecal contents of hens (HCC) fed mash or mash supplemented with MOS or PKM on the major microflora groups of chicks, and their inhibitory effect on Salmonella enterica serovar Enteritidis (PT4) colonization. Finally, this study investigates the effect over time of diets supplemented with MOS or PKM on S. Enteritidis colonization and the microflora of chicks. In hens, supplemented diets increased Bifidobacterium spp., while decreasing members of Enterobacteriaceae and Enterococcus spp., compared with the mash diet. Chicks dosed with the HCC showed, on average, increased numbers of anaerobes, while the numbers of aerobes decreased including coliforms and S. Enteritidis compared with controls without HCC. In chicks fed the MOS-supplemented or PKM-supplemented diets, S. Enteritidis colonization decreased over time, compared with mash alone. Four-week-old PKM birds showed an increase in Bifidobacterium spp. and Lactobacillus spp., with a decrease in S. Enteritidis compared with week 2. Generally, the HCC and diets supplemented with MOS or PKM affected the birds intestinal microflora by increasing the Bifidobacterium spp. and Lactobacillus spp., while decreasing the Enterobacteriaceae groups. They also reduced susceptibility in young chickens to colonization by S. Enteritidis.

Animal Feed↗
PubMed1996

Prevalence of HTLV type I infection in Iran: a serological and genetic study.

Several publications describe the presence of the human T cell lymphotropic virus type I (HTLV-I) in Jewish individuals born in Mash-had, Iran. We report here the results of HTLV-I serological and genetic studies in the non-Jewish population of Mash-had as well as a neighboring area: Gonbad-Kavous. Seven hundred and seven serum samples from Mash-had (694 healthy individuals and 13 patients with lymphoma) and 90 from Gonbad-Kavous were tested for HTLV antibodies by gelatin particle agglutination assay (PA) and confirmatory Western blots (WBs). Seropositive rates of 3.0% (21 of 694) in Mash-had, 0% (0 of 90) in Gonbad-Kavous, and 100% (13 of 13) in lymphoma cases were observed. HTLV-I DNA sequence were amplified by polymerase chain reaction directly from the fresh PBMCs of seropositive individuals. Phylogenetic analysis of the viral DNA sequence indicated that the HTLV-I present in Mash-had belong to the HTLV-I cosmopolitan clade. Altogether, these data indicate that Mash-had, located in northeastern Iran, is a newly recognized endemic center for HTLV-I.

Amino Acid Sequence↗
PubMed1997

Self-selection of dietary protein and energy by broilers grown under a tropical climate: effect of feed particle size on the feed choice.

Broilers, 2 wk of age, that had been previously adapted to energy: protein choice feeding, were offered corn (either ground, cracked, or presented as whole grains) and a protein concentrate (43.7% CP) in mash or pellet form. When corn was fed as whole grains, protein concentrate in the selected diet was significantly higher (35.1%) than with cracked corn (29.3%) or ground corn (29.1%). Presenting the concentrate as pellets resulted in a significantly higher concentration in the diet (32.7%) than when mash concentrate was fed (29.6%). Live BW at 4 and 6 wk of age were not significantly affected by feed texture. However, offering corn as whole grains or concentrate as pellets induced a significant improvement in feed efficiency. Total time to eat larger size particles (whole grains, pelleted concentrate) was significantly less than total time to eat ground corn or mash concentrate. Furthermore, the mean duration of the feeding bouts was two times shorter for whole grains (48 s) than for ground corn (98 s) and for pelleted concentrate (56 s) than for mash concentrate (114 s). Chickens ate whole grains or pellets at a significantly slower rate (number of pecks per second feeding time) than when eating ground corn or mash concentrate. There was a rejection during the first 24 h when the form of the concentrate (mash to pellets) was changed. Full adaptation to the new size of the concentrate required about 3 d.

Analysis of Variance↗
PubMed1992

What consistency of food is best for children with cerebral palsy who cannot chew?

Video recordings were made of 67 children with cerebral palsy and 64 able bodied children eating soft boiled ('non-mashed') and mashed potato. Those children with cerebral palsy who had no speech, presumed to have poor oral motor function, took significantly longer to eat non-mashed than mashed potato. Children with cerebral palsy, especially those with no speech, were more likely to cough or choke while eating non-mashed than mashed potato. It is recommended that children with cerebral palsy who have poor oral motor function are offered food that they can eat with the least frustration or distress. This may also improve their dietary intake and state of nutrition.

Adolescent↗
PubMed1993

Research note: effect of diet density and pelleting on productive parameters of Japanese quail.

An experiment was conducted to determine the response of growing Japanese quail to two dietary energy levels, high (3,200 kcal ME/kg) versus low (3,000 kcal ME/kg), and two forms of presentation, mash versus pellet. Seven thousand, two hundred day-old Japanese quail (Coturnix coturnix japonica) were randomly distributed into 24 floor pens containing 300 quail each. The experiment was divided in two phases: starter (until 2 wk of age) and finisher (Days 14 to 33). Weight gain was less (P < .05) for quail fed the low-energy mash diet than for those on other treatments. Feed consumption increased (P < .001) when low-energy and mash diets were fed. Metabolizable energy consumption was greater with the mash diets. Feed conversion improved (P < .001) when high-energy and pelleted diets were fed as compared with low-energy and mash diets, respectively. Results indicated that daily gain and feed conversion of quail fed the low-energy, pelleted diet equaled those of quail fed the high-energy mash diet. Pelleting improved the caloric efficiency of gain by quail. Quail seemed to adjust caloric intake according to the energy level of the diet.

Animal Feed↗
PubMed1986

Hospitals and self-help groups: opportunity and challenge.

Mutual aid self-help (MASH) groups can be a positive means of enabling persons to cope effectively with their health and personal problems. MASH groups provide emotional support, decrease anxiety, foster referrals to the hospital, reduce lengths of stay, and facilitate the discharged patient's reentry into the home and community. Yet this potentially valuable resource has been largely untapped by hospitals. Hospitals can take a number of steps to integrate their programs and services with MASH networks: Identify self-help groups in the hospital's service area. Establish liaison with groups that are of key interest to various hospital departments and programs. Make referrals to appropriate MASH groups as part of the discharge-planning process. Promote the development of MASH groups by appropriate hospital departments. Provide logistic support to self-help groups. Promote awareness of MASH Groups through specialized directories or tapes. Educate professional staff on the value, variety, and limitations of self-help groups. Provide consultation and speakers.

Attitude of Health Personnel↗
PubMed1990

Enrichment of nutrient quality of cassava (Manihot esculenta Crantz) with microbial proteins.

The effect of fermentation on the nutrient quality of cassava was investigated. Chemical analysis revealed a general increase in ash, fibre and crude protein content of the fermented cassava mash, while the carbohydrate content showed a substantial decrease. The results of the chemical analysis indicated that fermentation resulted in a slight protein enrichment of the fermentated cassava mash. When active yeast cells were inoculated into the cassava mash before fermentation, the yeast cells grew and generated additional cell mass which was reflected in a higher content of crude protein in the fermented cassava mash. Results obtained also revealed that 3.3% concentration of yeast cell inoculum added to cassava mash before commencement of fermentation was optimum for maximal crude protein formation.

Carbohydrates↗
PubMed1992

Decreased reactivity to sweetness following chronic exposure to mild unpredictable stress or acute administration of pimozide.

Consumption of a palatable wet mash was examined in rats subjected chronically (4-10 weeks) to unpredictable mild stress. Intake of mash containing 0, 10%, or 20% additional sucrose was normal in stressed animals. In control animals, the addition of 30% or 40% sucrose caused a decrease in the quantity of mash consumed, but increased the rate of eating. Both the increase in eating rate and the decrease in intake, at high sucrose concentration, were markedly attenuated in stressed animals (which therefore had higher intakes of very sweet mash and lower rates of eating, relative to control animals). Like chronic mild stress, the dopamine receptor antagonist pimozide (0.2 mg/kg) also increased the intake of a wet mash with 30% added sucrose, while decreasing the rate of consumption. Stressed animals were relatively insensitive to pimozide, though there were significant additive effects on duration of eating (increased) and on postprandial resting (suppressed). The failure of stressed animals to adapt their intake to increases in sweetness, and the similarities between the effects of chronic mild stress and acute pimozide, are compatible with the hypothesis that animals exposed to chronic mild stress are anhedonic.

Animals↗
PubMed2003

Saccharification of chitin using solid-state culture of Aspergillus sp. S1-13 with shellfish waste as a substrate.

Saccharification of chitin was performed in a suspension (mash) of a solid-state culture of chitinase-producing Aspergillus sp. Sl-13 with acid-treated shellfish waste as a substrate. The conditions for the saccharifying reaction and the solid-state cultivation were examined from the viewpoint of saccharification in the mash. Optimum cultivation conditions were defined: a solid-state medium consisting of 5 g of 10% lactic acid-treated crab shells (0.50-2.36 mm in size) and 3 ml of a basal medium (0.028% KH2PO4 0.007% CaCl2.2H2O, and 0.025% MgSO4.7H2O) supplemented with 0.3% peptone was inoculated with 4 ml of spore suspension (1 x 10(7) spores/ml), and the water content of the medium was adjusted to 75%; static cultivation at 37 degrees C for 7 d. When a culture obtained under the optimum conditions was suspended in 70 ml of 50 mM sodium phosphate-citrate buffer (pH 4.0) and incubated at 45 degrees C for 11-13 d, 55 mM N-acetylglucosamine (GlcNAc) was formed in the solid-state culture mash, indicating that at least 33% of the initial chitin in the solid material was hydrolyzed. Through the experiments, the amounts of G1cNAc formed in the solid-state culture mash varied in a way similar to that of the water-extractable pnitrophenyl beta-D-N-acetylglucosaminide-hydrolyzing enzyme in the culture, but not to that of the colloidal chitin-hydrolyzing enzyme. G1cNAc-assimilating lactic acid bacteria, which were inoculated into the mash after or at the start of the saccharification, formed lactic acid with decreasing GlcNAc.

Journal Article↗
PubMed1997

Use of virginiamycin to control the growth of lactic acid bacteria during alcohol fermentation.

The antibiotic virginiamycin was investigated for its effects on growth and lactic acid production by seven strains of lactobacilli during the alcoholic fermentation of wheat mash by yeast. The lowest concentration of virginiamycin tested (0.5 mg Lactrol kg-1 mash), was effective against most of the lactic acid bacteria under study, but Lactobacillus plantarum was not significantly inhibited at this concentration. The use of virginiamycin prevented or reduced potential yield losses of up to 11% of the produced ethanol due to the growth and metabolism of lactobacilli. However, when the same concentration of virginiamycin was added to mash not inoculated with yeast, Lactobacillus rhamnosus and L. paracasei grew after an extensive lag of 48 h and L. plantarum grew after a similar lag even in the presence of 2 mg virginiamycin kg-1 mash. Results showed a variation in sensitivity to virginiamycin between the different strains tested and also a possible reduction in effectiveness of virginiamycin over prolonged incubation in wheat mash, especially in the absence of yeast.

Anti-Bacterial Agents↗
PubMed2000

Effect of foraging material and food form on feather pecking in laying hens.

1. The aim was to test whether provision of foraging material and food form influence feather pecking and feather damage in laying hens. 2. From week 19 of age, 16 groups of 11 hens (white Lohman Selected Leghorn hybrids) were kept in pens with or without access to long-cut straw as foraging material and fed on either mash or pellets. 3. Foraging behaviour was increased in hens with access to straw and time spent feeding was increased in hens fed on mash. In addition, hens fed on mash had longer feeding bouts and higher rates of pecking at the food during feeding than hens fed on pellets. 4. There were interaction effects of foraging material and food form on both feather pecking and feather damage. High rates of feather pecking and pronounced feather damage were only found in hens housed without access to straw and fed on pellets. In groups characterised by high rates of feather pecking the hens also showed more severe forms of this behaviour. 5. Differences in the time budgets of hens kept in different housing conditions suggested that birds fed on mash used the food not only for feeding but also as a substrate for foraging behaviour. 6. In order to avoid problems with feather pecking it is recommended that laying hens are provided with foraging material and fed on mash.

Animal Feed↗
PubMed2000

Effect of diet form and enzyme supplementation on growth, efficiency and energy utilisation of wheat-based diets for broilers.

1. The effect of diet form (mash, cold-pelleted, steam-conditioned/pelleted, wet mash, whole wheat with balancer pellet, restricted pellet) and enzyme inclusion (Avizyme 1300, absent, present) was studied in 2 trials using individually caged, male broilers from 14 to 42 d. Bird performance, viscosity of ileal contents and diet metabolisability (AME) were measured. 2. The performance of mash-fed birds was significantly poorer than for the other treatments in relation to dry matter intake, liveweight gain and gain:food. This was not due to reduced diet AME content. 3. There was no significant effect of heat treatment on any of the variables measured, although viscosity of ileal contents was increased by 30% as compared to the cold-pelleted diet. 4. Gain:food was improved with wet-mash feeding in comparison to the dry mash treatment but it was concluded that this was not due to any intrinsic improvement in diet quality, but rather to voluntary food restriction on introduction of the wet food. 5. Whole wheat feeding improved gain:food and diet AME content by 3% as compared to the complete diets and caused approximately a 50% increase in gizzard weight as compared with the pelleted diets. 6. Food enzyme inclusion did not improve performance although a significant improvement in diet AME content was observed with enzyme inclusion in trial 1.

Amino Acids↗
PubMed2002

Feeding broiler chickens wheat and barley diets containing whole, ground and pelleted grain.

The effect of grain form (whole, mash, or pelleted) on the live performance of broiler chickens was determined. In the first trial, six regimens compared the feeding of whole wheat: 1) 0% of diet for the whole trial (0 to 48 d); 2) 5% at 6 d, 20% at 13 d, 35% at 27 d; 3) 5% at 6 d, 35% at 13 d, 50% at 27 d; 4) 5% at 0 d, 20% at 6 d, 35% at 13 d, 50% at 27 d; 5) 5% at 6 d, 50% at 13 d, 65% at 27 d; and 6) 5% at 0 d, 20% at 6 d, 50% at 13 d, 65% at 27d. Each feeding regimen was replicated with steam-pelleted and mash supplements. None of the feed was diluted. The second trial was similar, except that whole barley was fed instead of whole wheat. Feeding mash supplements slowed growth at all ages and lowered mortality caused by sudden death syndrome and ascites plus right heart failure. Cumulative feed:gain was increased by feeding mash supplements in Trial 1. Total weight gain was unaffected by feeding whole wheat but was decreased by most levels of whole barley. Whole-grain diets increased cumulative feed:gain. Feeding whole wheat decreased skeletal problems. Whole-grain diets increased gizzard size but did not alter carcass yield. Feeding whole-grain and mash supplements caused at least a temporary loss in growth rate and feed efficiency but in some cases improved bird health.

Animal Feed↗
PubMed2006

Effects of feed particle size and feed form on survival of Salmonella typhimurium in the alimentary tract and cecal S. typhimurium reduction in growing broilers.

An in vitro experiment and an in vivo experiment were conducted to investigate the effects of feed particle size (coarse and fine) and feed form (mash and pellet) on the survival of Salmonella typhimurium (ST) in the alimentary tract, and the reduction of cecal ST in growing broilers in a 2 x 2 factorial design. All diets in the 2 trials were corn and soybean meal-based diets that differed only in physical characteristics. Diets were provided for birds from d 1 to 22 of age. In the in vitro trial, the relative gizzard weight was lower in birds that were fed the pellet diet (P < 0.01), whereas the relative weight of the cecum was higher in these birds (P < 0.01). Broilers receiving the pellet diet had enhanced concentrations of volatile fatty acids in contents from both the gizzards and the ceca. In addition, there was an increase in gizzard pH (P < 0.01) with the pellet diet, but a reduction in cecal pH (P < 0.05). Pellet-fed birds showed a significantly decreased and increased in vitro death rate of ST in the contents from gizzards (P < 0.01) and ceca (P < 0.05), respectively. A higher in vitro ST death rate in the gizzard was observed in birds given the coarse mash diet compared with those given the fine mash diet. In the in vivo experiment, cecal volatile fatty acid concentrations were increased, whereas cecal pH was decreased significantly (P < 0.05) when birds were fed the pellet diet compared with the mash diet. Furthermore, cecal ST concentrations were higher (P < 0.05) in broilers fed the pellet diet than in those fed the mash diet. Results indicated that the pellet diet increases the incidence of ST in gizzards and ceca in growing broilers and provide evidence demonstrating that the gizzard may play a critical role in reducing ST contamination in growing broilers.

Animal Feed↗
PubMed2002

Generation of neuroendocrine chromaffin cells from sympathoadrenal progenitors: beyond the glucocorticoid hypothesis.

The developmental diversification of neural crest-derived sympathoadrenal (SA) progenitor cells into neuroendocrine adrenal chromaffin cells and sympathetic neurons has been thought to be largely understood. Based on two decades of in vitro studies with isolated SA progenitor and chromaffin cells, it was widely assumed that chromaffin cell development crucially depends on glucocorticoid hormones provided by adrenal cortical cells. However, analysis of mice lacking the glucocorticoid receptor has revealed that the chromaffin cell phenotype develops largely normally in these mice, except for the induction of the adrenaline synthesizing enzyme phenylethylamine N-methyl transferase. In a search for novel candidate genes that might be involved in triggering the sympathetic neuron/chromaffin cell decision, we have studied putative contributions of transforming growth factor (TGF)-alpha, BMP-4, and the transcription factor MASH-1, molecules with distinct expressions in SA progenitor cells, in their migratory pathways and final destinations. TGF-beta2 and -beta3 and BMP-4 are highly expressed in the wall of the dorsal aorta and in the adrenal anlagen during and after immigration of SA progenitors but expressed at much lower levels in sympathetic ganglia. We found that neutralizing antibodies against all three TGF-beta isoforms applied to the chorionic-allantoic membrane (CAM) of quail embryos interfere with proliferation of immigrated adrenal chromaffin cells but do not affect their specific neuroendocrine ultrastructural phenotype. Grafting of noggin-producing cells to the CAM, which scavenges BMPs, interferes with visceral arch and limb development but does not overtly affect the chromaffin phenotype. The transcription factor MASH-1 promotes early differentiation of SA progenitors. Mice deficient for MASH-1 lack sympathetic ganglia, whereas the adrenal medulla previously has been reported to be present. We show here that most adrenal medullary cells in MASH-1(-/-) mice identified by Phox2b immunoreactivity lack the catecholaminergic marker tyrosine hydroxylase. More surprisingly, most cells do not contain chromaffin granules and display a neuroblast-like ultrastructure and show strongly enhanced expression of c-RET comparable to that observed in sympathetic ganglia. Together, our data suggest that TGF-betas and BMP-4 do not seem to be essential for chromaffin cell differentiation. In contrast with previous reports, however, MASH-1 apparently plays a crucial role in chromaffin cell development.

Adrenal Glands↗
PubMedDate not supplied

Maternal consumption of Lake Ontario salmon in rats produces behavioral changes in the offspring.

The current study assessed the effects of maternal, paternal, or combined parental consumption of Lake Ontario salmon in rats on the behavior of their offspring. Adult female Sprague-Dawley rats were put on a 30 day diet of either ground rat chow containing 30% Lake Ontario salmon (LAKE) or 30% Pacific Ocean salmon (OCEAN). These females were then mated with adult male rats similarly exposed (LAKE or OCEAN). An additional control group of males and females who were fed ground rat chow (MASH) only were also mated. These pairing combinations resulted in five offspring groups: LAKE-LAKE, LAKE-OCEAN, OCEAN-LAKE, OCEAN-OCEAN, MASH-MASH. When the offspring reached 80 days of age, they were tested for reactivity to frustrative nonreward using runway successive negative contrast, which has been repeatedly shown to be increased in adult rats fed Ontario salmon. Consistent with previous work, results showed that the behavior of the OCEAN-OCEAN rats did not differ from the MASH-MASH group, indicating that a salmon diet per se does not cause behavioral change. However, the offspring of dams who consumed Lake Ontario salmon (LAKE-LAKE and OCEAN-LAKE) showed an increased depression effect relative to controls. There was little evidence of a paternal effect. A follow-up experiment employed cross-fostering to determine the relative contribution of pre- and/or postnatal exposure to Lake Ontario salmon consumption on offspring behavior. Rat pups were cross-fostered to or from dams who consumed Lake Ontario salmon during gestation and parturition. Results from two separate replications indicated that prenatal (LAKE to OCEAN) exposure alone or postnatal (OCEAN to LAKE) exposure alone produced a large increase in successive negative contrast relative to controls (OCEAN to OCEAN). These data are strong evidence of behavioral changes produced by maternal consumption of Lake Ontario salmon in the offspring rat. Further, they indicate that either prenatal or postnatal exposure alone is sufficient to produce behavioral changes in the offspring.

Animals↗
PubMed2006

Characterization of a beta-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry.

BACKGROUND: In the barley malting process, partial hydrolysis of beta-glucans begins with seed germination. However, the endogenous 1,3-1,4-beta-glucanases are heat inactivated, and the remaining high molecular weight beta-glucans may cause severe problems such as increased brewer mash viscosity and turbidity. Increased viscosity impairs pumping and filtration, resulting in lower efficiency, reduced yields of extracts, and lower filtration rates, as well as the appearance of gelatinous precipitates in the finished beer. Therefore, the use of exogenous beta-glucanases to reduce the beta-glucans already present in the malt barley is highly desirable. RESULTS: The zygomycete microfungus Rhizopus microsporus var. microsporus secreted substantial amounts of beta-glucanase in liquid culture medium containing 0.5% chitin. An active protein was isolated by gel filtration and ion exchange chromatographies of the beta-glucanase activity-containing culture supernatant. This isolated protein hydrolyzed 1,3-1,4-beta-glucan (barley beta-glucan), but showed only residual activity against 1,3-beta-glucan (laminarin), or no activity at all against 1,4-beta-glucan (cellulose), indicating that the R. microsporus var. microsporus enzyme is a member of the EC 3.2.1.73 category. The purified protein had a molecular mass of 33.7 kDa, as determined by mass spectrometry. The optimal pH and temperature for hydrolysis of 1,3-1,4-beta-glucan were in the ranges of 4-5, and 50-60 degrees C, respectively. The Km and Vmax values for hydrolysis of beta-glucan at pH 5.0 and 50 degrees C were 22.39 mg.mL-1 and 16.46 mg.min-1, respectively. The purified enzyme was highly sensitive to Cu+2, but showed less or no sensitivity to other divalent ions, and was able to reduce both the viscosity and the filtration time of a sample of brewer mash. In comparison to the values determined for the mash treated with two commercial glucanases, the relative viscosity value for the mash treated with the 1,3-1,4-beta-glucanase produced by R. microsporus var. microsporus. was determined to be consistently lower. CONCLUSION: The zygomycete microfungus R. microsporus var. microsporus produced a 1,3-1,4-beta-D-glucan 4-glucanhydrolase (EC 3.2.1.73) which is able to hydrolyze beta-D-glucan that contains both the 1,3- and 1,4-bonds (barley beta-glucans). Its molecular mass was 33.7 kDa. Maximum activity was detected at pH values in the range of 4-5, and temperatures in the range of 50-60 degrees C. The enzyme was able to reduce both the viscosity of the brewer mash and the filtration time, indicating its potential value for the brewing industry.

Beer↗