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[Disturbances in the digestive tract of Ips sexdentatus (Insecta: Scolitidae) induced by Parasitorhabditis ipsophila (Nematoda: Rhabditidae)].

The histological study of the digestive tract in Ips sexdentatus parasitized by P. ipsophila showed major destruction of the microvillosities as well as vacuolization of the epithelial cells in the terminal part of the mesenteron. No damage could be observed in the hind gut nor during low contamination. Concurrently, the biochemical study indicated a lower amount of proteins in the alimentary bolus of parasitized insects along with a higher amount of proteins in the intestinal wall. Damage seemed chiefly mechanical and accounted for the disorders previously observed in populations. The apparent contradiction between the magnitude of damage to the intestinal epithelium and the mild effects of parasitism on host populations might be explained by the preferential location of nematodes in the terminal part of the mesenteron and the proximal part of the hind gut.

Animals↗

Lipid transport through the enterocytes of larval Aeshna cyanea (Insecta, Odonata).

The larval enterocytes of A. cyanea absorb lipid after luminal lipolysis in morphologically invisible form by direct membrane transport, presumably molecular diffusion. The lipolytic products are utilized for resynthesis of di- and triglyceride which become visible in the form of lipid droplets in the groundplasm. The putative site of lipid synthesis is the apical ER which locally forms highly ordered complexes. Lipid transport occurs in the form of matrix lipid so that the enterocytes of dragonfly larvae resemble in this respect the lipid-secreting mammocytes rather than the lipid-absorbing mammalian enterocytes. Lipid release involves partial lipolysis and direct membrane transport, possibly including membrane delamination, again in contrast to exocytosis in mammalian enterocytes and apocrine extrusion in mammocytes, which are both indirect membrane transport mechanisms.

Animals↗

[Heterogeneity and homologies of the repeating and unique DNA of dragonflies (Odonata, Insecta)].

A relative content of unique and reiterated nucleotide sequences in DNA of eleven dragonfly species was estimated. The degree of intra- and intergenomic divergence of these DNA sequences was determined by means of DNA-DNA hybridization. Species from different genera share 40-45% of the repetitive sequences and those from different families--from 11 to 20% only. Data on the thermostability of homo- and heteroduplexes suggest that new families of the repetitive sequences have arisen repeatedly during dragonflies evolution. The quality of homologous unique sequences in the DNA compared (20-97%) correlates with the taxonomic relationships of species. Phylogenesis of some dragonfly families is discussed in view of the results obtained.

Animals↗