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At least 217 records · Page 12Linked to original sources
PubMed1990

Cytosol progesterone and 17 alpha-hydroxyprogesterone levels and luteinizing hormone and chorionic gonadotropin receptors in human corpora lutea.

Cytosol progesterone (P) and 17 alpha-hydroxyprogesterone (17-OHP) levels and luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptors were measured in 27 corpora lutea and four corpora albicantia. Cytosol P concentrations were highest in corpora lutea (mean +/- SEM, 3.1 +/- 0.8 micrograms/g) during the midluteal phase (days 15 to 19) rather than the early (2.2 +/- 0.8 micrograms/g, days 20 to 25) and late luteal phases (1.8 +/- 0.8 micrograms/g, days 26 to 30). Cytosol 17-OHP concentrations also were 3.3 +/- 0.5, 4.3 +/- 0.6, and 3.3 +/- 1.0 micrograms/g in early, midluteal, and late luteal phases, respectively, and was significantly inversely correlated with occupied LH/hCG receptors in midluteal phase. Corpora albicantia had the lowest P (0.3 +/- 0.05 microgram/g) and 17-OHP (0.9 +/- 0.6 micrograms/g) concentrations. Cytosol P and 17-OHP may therefore reflect the balance between the luteal cell production and secretion, whereas the amount of occupied and unoccupied LH/hCG receptors may partially explain the relationship between LH and P secretion.

17-alpha-Hydroxyprogesterone↗
PubMed1990

Erythema multiforme associated with autoreactivity to 17 alpha-hydroxyprogesterone.

We report a case of a 23-year-old woman who was afflicted with disseminated skin erythema multiforme-like eruptions that started at the menarche, relapsed at the premenstrual periods, dramatically spread during two pregnancies and cleared after abortion; the skin lesions responded dramatically to thalidomide treatment. A high-affinity binding factor to 17 alpha-hydroxyprogesterone (17-OHP) was found in the serum of this patient. Her lymphocytes did not proliferate in vitro after exposure to exogenous 17-OHP but showed significant chromatin activation. There was a decreased expression of HLA antigens at the surface of the patient's blood lymphocytes. This is a unique well-documented case of erythema multiforme most possibly due to autoreactivity to 17-OHP; the precise mechanism(s) of this autoreactivity has not been established.

17-alpha-Hydroxyprogesterone↗
PubMed1989

[Analysis of plasma levels of dehydroepiandrosterone and 17-hydroxyprogesterone in physiologic and pathologic pregnancy and labor].

Plasma levels of dehydroepiandrosterone (DEA) and 17-hydroxyprogesterone (17-HP) were studied in 43 pregnant females 11 with normal pregnancy and labor and 32 at high risk of obstetric complications (primary powerless labor, prematurity, postmaturity, rapid parturition). Hormonal investigation was performed in each particular female in the time-course of her pregnancy and labor as well as in the mixed blood plasma from the umbilical cord. Concentrations of hormones observed in the females with normal pregnancies and labors differed from those in the patients whose pregnancy was complicated as well as their labor. It has been concluded that the results of DEA and 17-HP variation assessment in the blood plasma are essential additional indicators of adrenal performance both in mother and fetus.

17-alpha-Hydroxyprogesterone↗
PubMedDate not supplied

[Pharmacology of new gestagens: derivatives of 17-alpha-hydroxyprogesterone].

The experimental data on the pharmacological activity of the modified analogs++ of 17 alpha-hydroxyprogesterone are presented. A high gestagenic activity was shown to be related to the presence of the methyl group in position 6, the double bond in position C6-C7, the hydroxyl and acetoxy groups in position 3. Such modification of the molecule provides a high degree of the secretory degeneration of the female rabbit endometrium at any method of administration as compared with progesterone but not always provides a high level of pregnancy preservation. Among the studied compounds, mepregenol diacetate with the acetoxy++ groups in positions 3 and 17 alpha is the most active in the ability of the secretory degeneration of the endometrium. The compound served as the basis for the development of the drug acetomepregenol possessing contraceptive and therapeutic effects.

17-alpha-Hydroxyprogesterone↗
PubMed1988

Nonspecificity of a direct 17 alpha-hydroxyprogesterone radioimmunoassay kit when used with samples from neonates.

We analyzed 240 samples for 17 alpha-hydroxyprogesterone (17-OHP) with the direct-assay kit ("Coat-A-Count" method for serum samples) from Diagnostic Products Corp. (DPC). The specimens were from 50 patients with known or suspected congenital adrenal hyperplasia (CAH); 74 mostly hospitalized neonates and infants, ages three days to three months; and 116 other patients, ages six months to 23 years. Samples from the CAH group were also analyzed with our in-house assay. For 39 of the neonatal samples, the analysis with the DPC assay was repeated with re-solubilized material that had been extracted from the serum with organic solvents. Values for "17-OHP" measured with the DPC direct assay were high, not only in CAH patients, but also in many of the unaffected neonates and infants. The extraction properties of the cross-reacting immunoreactive material into various organic solvent systems were different from those of 17-OHP, and were more like those of steroid sulfates. Because of this significant cross-reactivity, we recommend that the DPC kit not be used for sera from children younger than six months of age, unless the method is modified to include an extraction step.

17-alpha-Hydroxyprogesterone↗
PubMed1985

The effect of treatment with defined spleen dialysate on plasma levels of 21-deoxycortisol and 17-hydroxyprogesterone in women with polycystic ovary disease.

Based upon the use of a specific radioimmunoassay method, the plasma levels of 21-deoxycortisol (21-DF) as well as of 17-hydroxyprogesterone (17- OHP) were examined in women with polycystic ovary disease - in normal conditions and after stimulation with corticotropin and chorionic gonadotrophin. It was shown that there was an increased level of 21-DF in women with PCOD in comparison with the control group, both in normal conditions and after ACTH stimulation. Besides there was a pathological increase in the level of 21-DF after HCG stimulation, which was not observed in the control group. These results suggest an enzymatic defect ("11-aberrant hydroxylation") in PCOD, which is due to a disturbance of steroidogenesis. Based upon the positive experiences with a defined spleen dialysate ("DSD", Solcosplen) in the treatment of climacteric discomforts and its known influence on steroidogenesis, we examined possible effects on the levels of 21-DF in females with PCOD. The decrease in the level of this steroid was associated with bleeding in women with amenorrhea. This shows the importance of 21-DF in the pathogenesis of PCOD and proves the efficacy of the spleen dialysate in women suffering from this disease.

17-Hydroxycorticosteroids↗
PubMedDate not supplied

Determination of 17-hydroxyprogesterone in the normal and induced ovulatory cycles.

Concentrations of 17-hydroxyprogesterone (170HPg) were measured in serum samples obtained daily through cycles both spontaneous and induced by gonadotropins. These samples had previously been assayed for estradiol, progesterone, luteinizing hormone (LH). The concentration of 170HPg increases considerably the day before the LH peak in the ovulatory cycles. In the anovulatory cycles the 170HPg levels are shown as being low or the constant high levels in one case has not been followed by the LH peak. In our opinion the parameter taken into consideration does not lend itself completely to the prediction of the ovulatory event but in synchronism with the LH peak, may be a useful index of the follicular luteinization both in the spontaneous ovulatory cycles and in the stimulated cycles where the estrogenic increase is abnormal.

17-alpha-Hydroxyprogesterone↗
PubMed1985

Direct solid-phase radioimmunoassay for screening 17 alpha-hydroxyprogesterone in whole-blood samples from newborns.

We describe a direct, solid-phase RIA for 17 alpha-hydroxyprogesterone (17-OH-P) that we are using to screen neonates for congenital adrenal hyperplasia. Phosphate buffer containing danazol and anti-17-OH-P is placed in tubes coated with antibody to IgG. The tubes also contain standards, controls, or blood samples on filter paper discs 3 mm in diameter. 125I-labeled 17-OH-P is added to each tube. The mixture is vortex-mixed and incubated overnight. The fluid and disc are removed, the radioactivity remaining in the tubes is counted, and the amount of 17-OH-P per disc is calculated by using a log-logit transformation of the standard curve. Results compare favorably with those by two extraction assays. Inter- and intra-assay CVs were less than 11% and less than 9%, respectively. Sensitivity was 2 pg per assay tube. There is no significant cross reactivity with structurally related steroids at their physiological concentrations. Analytical recovery of added 17-OH-P averaged 104%. 17-OH-P in whole blood spotted on filter paper is stable for at least six months.

17-alpha-Hydroxypregnenolone↗
PubMed1987

[Comparison of the kinetics of the response of salivary and plasma 17-hydroxyprogesterone to the administration of HCG in men].

17-hydroxyprogesterone (17-OHP) time course response to hCG (5,000 IU) was studied simultaneously in the saliva and the plasma of 12 adult healthy men. In both fluids, 17-OHP was determined by radioimmunossay after chromatography on Sephadex LH-20 columns of diethyl ether extracts. The use of an iodinated tracer has greatly increased the sensitivity of the technique. Baseline levels in plasma and saliva were: 1.0 +/- 0.1 ng/ml (mean +/- SEM) and 24 +/- 2 pg/ml respectively. After hCG, a biphasic pattern was observed in both fluids with a similar early response but the peak elicited at 33 h in plasma was not observed in saliva where the levels were lower than those recorded at 24 h. At 48 h saliva 17-OHP increased again to levels similar to those at 24 h. Thus the 17-OHP pattern in saliva was the mirror image of that found in plasma. Since saliva steroids are believed to reflect the plasma non-protein bound fraction, this difference was assumed to be due to the decrease of the unbound fraction of plasma 17-OHP in the late afternoon as a consequence of the increase of the CBG-bound fraction since at that time cortisol levels are low. Indeed the ratios of saliva to plasma 17-OHP levels were found to be significantly correlated with plasma cortisol levels: r = 0.44 (p less than 0.01; n = 140). Thus the absence of the secondary peak at 33 h may be due to cortisol circadian rhythm. However the similar response in saliva at 24 and at 48 h after hCG was enough important to make reliable the evaluation of the endocrine testicular function so that saliva may be collected instead of plasma in the hCG stimulation test protocol.

17-alpha-Hydroxyprogesterone↗
PubMed1987

[Neonatal screening for congenital adrenal hyperplasia due to 21-hydroxylase deficiency. 3. An enzyme-linked immunosorbent assay for dried blood 17 alpha-hydroxyprogesterone].

An enzyme-linked immunosorbent assay (ELISA) for 17 alpha-hydroxyprogesterone (17-OHP) in dried blood collected on filter paper has been developed. The ELISA method is very easy and rapid and has sensitivity, accuracy and precision. The detection limit of the ELISA method is 0.5 ng/ml blood. Intra- and interassay coefficients of variation are 7.0 approximately 8.3% and 6.7 approximately 10.5%, respectively. Correlation between the ELISA method and previously reported enzyme immunoassay is good (r = 0.961). 17-OHP values of neonates with congenital adrenal hyperplasia (CAH) are extremely high compared with normal neonates (11.0 +/- 5.9 ng/ml blood). The present study demonstrates that the ELISA method for 17-OHP is applicable to neonatal screening for CAH.

17-alpha-Hydroxyprogesterone↗
PubMed1987

Enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.

In this rapid, cost-effective, enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone (17-OHP) eluted from dried blood spotted on filter paper, second antibody is coated onto the microwell plate and horseradish peroxidase (EC 1.11.1.7) is the label enzyme. Antiserum to 17-OHP was prepared by using 4-(2-carboxymethylthio)-17-OHP-bovine serum albumin conjugate as immunogen. Enzyme conjugate was prepared from 4-(2-carboxymethylthio)-17-OHP and peroxidase. The blood spots are assayed in the microwells without extraction or centrifugation steps. The detection limit of the assay is 1 microgram/L, equivalent to 3.5 pg (10.6 fmol) per disc. Intra- and interassay CVs at two steroid concentrations (7.38 and 22.79 micrograms/L) ranged from 3.74 to 11.90% (n = 5), and 9.49 and 9.83% (n = 5), respectively. Results correlated well (r = 0.91) with those of a fluorescence enzyme immunoassay. The sensitivity, specificity, and precision of this method make it potentially useful in the mass screening of neonates for congenital adrenal hyperplasia.

17-alpha-Hydroxyprogesterone↗
PubMed1985

Progesterone and 17 alpha-hydroxyprogesterone advance the estrogen-induced bioassayable luteinizing hormone surge in castrate monkeys.

Recent studies have demonstrated marked disparities between bioassayable (BIO) versus radioimmunoassayable concentrations of luteinizing hormone (LH) at midcycle. Other studies in intact women and monkeys have indicated that progesterone (P) may facilitate estrogen induction of the preovulatory gonadotropin surges. We have combined the study of these observations as follows: long-term castrate female monkeys were given estradiol benzoate with and without subsequent P or 17 alpha-hydroxyprogesterone (17-OHP) injections. Plasma was collected during a selected 24-hour interval via a chronic indwelling femoral vein cannula. Initially, estrogen-negative feedback decreased the pulsatility and circulating levels of gonadotropins. P and 17-OHP treatment (after estradiol) hastened initiation of the BIO-LH surge by up to 8 hours. Although estrogen-positive feedback alone enhanced the biologic activity of LH, P and 17-OHP expedited the midcycle-like BIO-LH surges.

17-alpha-Hydroxyprogesterone↗
PubMed1982

Enzyme immunoassay of 17-hydroxyprogesterone in plasma, microfilter paper blood and saliva of newborns, children and patients with congenital adrenal hyperplasia.

An enzyme immunoassay for the measurement of 17-hydroxyprogesterone (OHP) is described. The antigens, OHP-3-CMO-BSA and 11-deoxycortisol-21-hemisuccinate-BSA have been used to produce antisera in rabbits. The enzyme marker was horseradish peroxidase. Bound/free separation was achieved by the double antibody/polyethylene glycol method. The method was used for plasma samples, capillary blood dried on paper disc and for saliva. The normal values for OHP in newborns and children were determined. This method makes possible a selected screening program for congenital adrenal hyperplasia in the newborns.

17-alpha-Hydroxyprogesterone↗
PubMed1982

Amniotic fluid levels of 17 alpha-hydroxyprogesterone during human pregnancy: pre-natal diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency.

Levels of 17 alpha-hydroxyprogesterone were estimated by radioimmunoassay in 174 amniotic fluid specimens obtained from 162 normal pregnancies of 16-20 weeks gestation. Another 18 specimens from 11 normal pregnancies between 25-42 weeks gestation and 7 specimens from 7 women who each previously had given birth to a child affected with CAH were also studied. There was no sex difference in AF 17 alpha-OH-progesterone levels, nor any significant downward trend in concentrations throughout the observed gestation period. Among the 7 subjects with previous history of CAH offsprings, 4 demonstrated normal levels of AF 17 alpha-OH-progesterone and 3 significantly higher values. HLA-typing of the cultivated amniotic cells confirmed these 4 cases to be heterozygous for 21-hydroxylase deficiency. The three subjects with high AF 17 alpha-OH-progesterone levels delivered babies affected with CAH, although HLA-typing in one case was non-conclusive. The failure of HLA-typing in 1 case for a confirmatory diagnosis indicates that the prenatal diagnosis of CAH must continue to rest on joint endocrinological and genetical investigations.

17-alpha-Hydroxyprogesterone↗
PubMed1980

Inefficacy of 17 alpha-hydroxyprogesterone caproate in the prevention of prematurity in twin pregnancy.

There are indications that prophylactic administration of 17 alpha-hydroxyprogesterone caproate (17 alpha-OHP-C) could be beneficial in the treatment of women at risk for preterm delivery. Since twin pregnancy is commonly associated with prematurity, 77 women with twin pregnancy were treated during the last trimester until the 37th gestational week with weekly injections of either 17 alpha-OHP-C or a placebo, following double-blind principles. The gestational length and birth weight and the outcome of the neonates were similar in both groups. The administration of 17 alpha-OHP-C thus seems ineffective in the prevention of prematurity risks associated with twin pregnancy.

17 alpha-Hydroxyprogesterone Caproate↗
PubMed1994

[Changes in reproductive hormones levels in the treatment of endometrial precancerous lesion with hydroxyprogesterone caproate].

The efficacy of progesterone in treating patients with endometrial carcinoma is well known. The mechanism, however, has not been understood yet. In this paper, 14 cases of precancerous lesion of endometrium were treated with hydroxyprogesterone caproate and a series of hormone determination was analysed before and after treatment. Results showed that LH and LH/FSH were dramatically decreased. (LH P < 0.05, LH/FSH P < 0.01). It is suggested that the mechanism of progesterone is multiphase, one of them is influence on pituitary and selective interference of LH and LH/FSH.

17 alpha-Hydroxyprogesterone Caproate↗
PubMed1996

Screening for congenital adrenal hyperplasia: the Delfia Screening Test overestimates serum 17-hydroxyprogesterone in preterm infants.

OBJECTIVE: To compare 17-hydroxyprogesterone (17-OHP) levels measured by quantitative serum radioimmunoassay (RIA), including an extraction step, and by screening fluoroimmunoassay (FIA) on blood spots in preterm infants. METHODS: Subjects were 39 healthy infants born at less than 31 weeks' gestational age. Each infant had weekly blood sampling, and RIA and FIA were performed on each sample. RESULTS: Two hundred twenty-seven samples were taken at 28 to 41 weeks' postconceptional age. Mean +/- SD 17-OHP measured by RIA was 11.4 +/- 11.1 nmol/L (0.4 +/- 0.4 micrograms/dL), and decreased over time. Mean +/- SD 17-OHP measured by FIA was 38.96 +/- 37.3 nmol/L, greater than 17-OHP (RIA). Log(delta FIA-RIA) was inversely related to postconceptional age (R2 = .39). CONCLUSION: Screening FIA of blood spots overestimates levels of 17-OHP in preterm infants and should not be used to determine the likelihood of congenital adrenal hyperplasia in this population. We have abandoned FIA screening for congenital adrenal hyperplasia in infants weighing less than 1500 g.

17-alpha-Hydroxyprogesterone↗
PubMed1997

17-alpha-Hydroxyprogesterone caproate and natural progesterone in assisted reproduction: a comparative study.

The use of luteal phase support has been demonstrated in patients undergoing an IVF/ET procedure. This study was designed to compare the absorption and the efficacy of two different luteal supports: 17-alpha-hydroxyprogesterone caproate (Lentogest, AMSA, Italy) and natural Progesterone (Prontogest, AMSA, Italy). A total of 80 patients received luteal supplementation with 50 mg of natural P/day intramuscularly, until beta-hCG evaluation. Then, in case of positive beta-hCG, patients were randomly divided into two groups (A and B) in order to compare two different protocols: Group A, 17-OHPc (341 mg once a week) and Group B, natural P (50 mg/day) both intramuscularly and extended for 10-12 weeks. Our study showed that the treatment with 17-OHPc results in a higher percentage of pregnancy rate compared to natural P, but the differences are not statistically significant. Thus, we emphasize that 17-OHPc preparation for better acceptance appears to be the most suitable and comfortable method for luteal phase support.

17 alpha-Hydroxyprogesterone Caproate↗