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Total joint replacement and golf.

Definitive characterization of the effects of golf on a total joint replacement remains elusive. However, from our study of active golfers and solicitation of the opinions of orthopedists who specialize in joint replacements, and from a careful review of the literature regarding exercise and total joint replacement, it appears possible for patients to remain active golfers with no significant increase in either symptoms or rates of revision of implants following total hip, knee, or shoulder replacement.

Arthroplasty↗

Molecular analysis of the multiple Golf alpha subunit mRNAs in the rat brain.

The alpha subunit of GTP-binding protein Golf (G alpha olf), identified in the olfactory epithelium, in which it is supposed to mediate odorant-generated adenylyl cyclase activations, is much more expressed in the striatum than G alpha s, the classical stimulatory G protein alpha subunit (Hervé et al., J Neurosci., 13 (1993) 2237-2248). Four species of G alpha olf messenger RNA (mRNA) were observed by Northern blot in the rat striatum. Analysis of striatal G alpha olf cDNA clones demonstrated that despite extensive variations in their 5' and 3' untranslated regions, these four G alpha olf mRNAs encode the same G alpha olf polypeptide. One of the four mRNA species, which was selectively observed in the striatum, is generated by a novel promoter whose activity was barely detectable in the olfactory epithelium. Surprisingly, this promoter generates an unexpectedly high proportion of transcripts in which the first intron is unspliced. The retention of intron was found to abolish the translation of G alpha olf mRNA in the reticulocyte lysate system, suggesting that it could be involved in the down regulation of G alpha olf expression in the striatum. Furthermore, a new polyadenylation site with a non canonical sequence, AATACA, was found to be responsible of the two shortest forms of G alpha olf mRNA. In conclusion, we have shown that the G alpha olf proteins present in the striatum and the olfactory epithelium are identical and that multiple variations in the untranslated parts of the mRNAs could affect G alpha olf expression.

Animals↗

The mechanism of fatal cardiopulmonary injury caused by a blow from a golf club.

In the course of a scuffle, a youth of 18 years was struck a single blow across the chest with a golf club. He collapsed dead on the spot. At autopsy, a precisely located and highly distinctive patterned imprint abrasion of the precordial region was revealed providing an excellent match with the weapon. The heart showed multiple internal ruptures and there was a laceration of the lingula of the left lung. Injury to the chest wall was confined to the soft tissues, the rib cage and sternum being intact. We postulate a novel mechanism to explain the cardiac rupture.

Adolescent↗

Ultrastructural localization of olfactory transduction components: the G protein subunit Golf alpha and type III adenylyl cyclase.

Electron microscopy and postembedding immunocytochemistry on rapidly frozen, freeze-substituted specimens of rat olfactory epithelia were used to study the subcellular localization of the transduction proteins Golf alpha and type III adenylyl cyclase. Antibody binding sites for both of these proteins occur in the same receptor cell compartments, the distal segments of the olfactory cilia. These segments line the boundary between organism and external environment inside the olfactory part of the nasal cavity. Therefore, they are the receptor cell regions that most likely first encounter odorous compounds. The results presented here provide direct evidence to support the conclusion that the distal segments of the cilia contain the sites of the early events of olfactory transduction.

Adenylyl Cyclases↗

Undiagnosed bronchial foreign body-golf tee.

A case of long-standing non-opaque foreign body (golf tee) which was removed from the right bronchus of a 15-year-old boy is reported. The importance of early diagnosis is stressed.

Adolescent↗

[Metazoan parasites of Boops boops (Linnaeus, 1758) (Teleostean Sparidae) in the "Golfe du Lion", faunistic and ecology (author's transl)].

A global study of the Metazoan parasites of Boops boops (L., 1758) have been made in the "golfe du Lion". Fourteen different species (eight Platyhelmintha, one Nematoda and five Crustacea) have been inventorized. Their respective localisation on the hosts, globals and specifics corresponding prevalences as well as their variations according to the size of the fish, and their abundance have been precised. Parasitics associations have also been examined.

Animals↗

Golf tee hairs in Netherton disease.

We present a case of Netherton disease, where the hairs lacked the characteristic microscopic feature of trichorrhexis invaginata. In its place were certain hairs with a golf tee morphology. These represent the proximal half of the invaginate node seen in typical Netherton disease. Scanning electron microscopy demonstrated the three-dimensional quality of this abnormality, which is subtle when assessed by light microscopy alone. We describe this sign so that it might be recognized when seen in isolation, as here, and allow the diagnosis of Netherton disease to be confirmed.

Child, Preschool↗

Local antibody production against the murine toxin of Yersinia pestis in a golf ball-induced granuloma.

The method of Garra and Baygorria, for localized antibody production, has been adapted for obtaining high-titered and monospecific antibodies against the murine toxin of Yersinia pestis. A hollow, perforated plastic golf ball surgically implanted under the skin of rabbits induced the formation of a granuloma. When the murine toxin of Y. pestis was injected directly into the granulomatous cavity, an increased amount of antibody was found in the granuloma fluid as compared with serum or with serum antibody obtained by conventional immunization. The granuloma antibody consisted mainly of immunoglobulin G, probably produced locally by lymphoid cells of the granuloma. The immune granuloma fluid and the granuloma tissue were rich in plasma cells and lymphocytes. The chemical composition of the granuloma fluid indicated that it was a transudate.

Animals↗

Thatch biodegradation and antifungal activities of two lignocellulolytic Streptomyces strains in laboratory cultures and in golf green turfgrass.

The use of lignocellulolytic Streptomyces spp. as biological agents, to enhance thatch degradation in turf and to slow its rate of accumulation while controlling fungal growth in the thatch layer, was studied. In flask scale studies, two lignocellulolytic Streptomyces violaceusniger (= hygroscopicus) strains (YCED9 and WYE53) decomposed thatch (> 30% dry weight) over a 12-week incubation period. Biodegradation was accompanied by production of extracellular cellulases, xylanases, and peroxidases. The accumulation of the polymeric, water-soluble lignin degradation intermediate acid, precipitable polymeric lignin (APPL), was also observed. Residual thatch from 12-week-old cultures had an increased lignin-to-carbohydrate ratio, an indication that although lignin was metabolized, carbohydrates were preferential carbon sources for these actinomycetes. A spore-containing soluble dry powder formulation was used as an inoculum in an in situ field experiment. This formulation was maintained in storage at 4 degrees C for over two years without viability loss. Results from the golf green experiment showed that although treated thatch layers in established greens were not appreciably reduced over the course of one summer, the Streptomyces were active and maintained their populations within the thatch, while fungal growth was suppressed as compared to controls. The results show that treatment of turfgrass with these Streptomyces may be useful for the long-term control of fungal populations within the thatch. Longer field studies are required to assess the long-term potential for also controlling thatch build-up and fungal pathogens.

2,4-Dichlorophenoxyacetic Acid↗

Production of polyclonal antibodies in rabbits is simplified using perforated plastic golf balls.

Production of polyclonal antibodies in the lumen of a perforated golf ball implanted surgically under the skin of a rabbit offers advantages over conventional techniques. Less stress is placed on the rabbit because bleeding is eliminated, complete adjuvants are not used and animal handling is minimized. The technique also offers the advantage that large amounts of antibody-containing fluid can be removed easily from the ball. In this report we describe the surgical protocol and demonstrate use of this technique to produce high-titered antibodies to plant and plant viral proteins.

Animals↗

Teaching nursing theory: a walk around the golf course.

What do golf courses, shopping malls, and neighborhoods have to do with teaching a graduate level nursing theory course? Walks around familiar places can help students see how the concrete becomes abstract in the process of theory development. Walks also can help students begin to see which elements or concepts are essential to the particular theory being discussed.

Education, Nursing, Graduate↗