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Digestive system.

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Digestive System Physiological Phenomena↗

Histochemical localization of glucose-6-phosphatase and 5-nucleotidase in the digestive system of Ophiocephalus Channa punctatus.

The histochemical localization of G6Pase and 5-Nase in the digestive system of Ophiocephalus (Channa) punctatus was studied. The highest activities of these enzymes were found in the liver. Appreciable activity was also found in the anterior intestine (duodenum) and pyloric caeca. The activity faded toward the middle and posterior intestine and rectum. In the stomach the activity was moderate. The activity of 5-Nase was weaker than that of G6Pase. In the stomach the enzymes were localized in the mucosa and gastric glands. The absorptive columnar epithelial cells were the major sites of localization in the intestine. The goblet cells were negative. The G6Pase activity was associated with the cytoplasm, while the 5-Nase activity was found in the cell membranes and the nuclei.

Animals↗

The endocrine cells of the digestive system: amines, peptides, and modes of action.

The endocrine cells of the digestive system (entero-endocrine cells of gastro-intestinal epithelia and Langerhans' islets of the pancreas) and the chemical messengers produced by them constitute a complicated and complex system. The physiological function of this system is the regulation of all processes related to digestion and resorption, and to homeostasis of carbohydrate metabolism. Using morphological and histochemical features of this cellular community, the present review deals with amines and amine metabolism, polypeptides and their immunohistochemical identification, and with the modes of action of enteric and pancreatic hormones. Special attention is paid to the significance of amine precursor uptake and decarboxylation (APUD), to immunohistochemical methodology and the interpretation of immunohistochemical findings, and to local regulatory mechanisms, especially paracrinia. Finally, unifying concepts for the integration of these cells and similar endocrine cells of other organs into a common system are considered.

APUD Cells↗

Histochemical localization of adenosine triphosphatase and thiamine pyrophosphatase in the digestive system of a teleost fish, Ophiocephalus punctatus.

The localization of ATPase and thiamine pyrophosphatase in the digestive system of Ophiocephalus punctatus has been studied. In stomach ATPase is found in the free border of the mucosa, gastric glands, submucosal connective tissue nuclei and muscularis. Thiamine pyrophosphatase is localized only in the mucosa and gastric gland cells. In the intestine, pyloric caeca and rectum, ATPase is distributed along the brush border of the columnar epithelial cells, their nuclei and cytoplasm. Mild activity is also found in the nuclei of submucosa and muscularis. The activity is stronger in the intestine than in the other portions. Thiamine pyrophosphatase activity in these portions is restircted only to the goblet cells. In the liver ATPase activity is associated both with the cytoplasm and nucleus of the hepatic cells. Thiamine pyrophosphatase activity is maximum in the centro-lobular portion.

Adenosine Triphosphatases↗