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PubMed1994

Determination and Distribution of cry-Type Genes of Bacillus thuringiensis Isolates from Taiwan.

Using PCR with a set of specific oligonucleotide primers to detect cryI-type genes, we were able to screen the cry-type genes of 225 Bacillus thuringiensis soil isolates from Taiwan without much cost in time or labor. Some combinations of cry genes (the cry-type profile) in a single isolate were unique. We identified five distinct profiles of crystal genes from the B. thuringiensis soil isolates from Taiwan. The cry genes included cryIA(a), cryIA(b), cryIA(c), cryIC, cryID, and cryIV. Interestingly, 501 B. thuringiensis isolates (93.5% of the total number that we identified) were isolated from areas at high altitudes. The profiles of cry-type genes were distinct in all isolation areas. The distribution of cry-type genes of our isolates therefore depended on geography. Using PCR footprinting to detect cryIC-type genes, we identified two distinct cryIC footprints from some of our isolates, indicating that these isolates may contain novel cryIC-type genes. B. thuringiensis isolates containing cryIA(a)-, cryIA(b)-, and cryIA(c)-type genes exhibited much greater activity against Plutella xylostella than did other isolates, indicating that multiple cry-type genes may be used as markers for the prediction of insecticidal activities.

Journal Article↗
PubMed1996

Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA.

Two pairs of universal oligonucleotide primers were designed to probe the most conserved regions of all known cryI-type gene sequences so that the amplified PCR fragments of the DNA template from Bacillus thuringiensis strains may contain all possible cryI-type gene sequences. The restriction fragment length polymorphism (RFLP) patterns of the PCR-amplified fragments revealed that 14 distinct cry-type genes have been identified from 20 B. thuringiensis strains. Those cry-type genes included cryIA(a), cryIA(a), cryIA(b), cryIA(b), cryIA(c), cryIB, cryIC, cryIC, cryIC(b), cryID, cryIE, cryIF, cryIF, and cryIII (a dagger at the end of a gene designation indicates a novel cry-type gene determined by restriction mapping or DNA sequences). Among them, the sequences of cryIA(a), cryIA(b), cryIB, cryIC, cryIF, and cryIII were found to be different from the corresponding published cry gene sequences. Interestingly, five cry-type genes [cryIA(a)-, cryIB-, cryIC-, cryIC(b)-, and cryIF-type genes] and seven cry-type genes [cryIA(a)-, cryIA(b)-, cryIB-, cryIC-, cryIC(b)-, cryIF-, and cryIII-type genes] have been detected from B. thuringiensis subsp. morrisoni HD-12 and B. thuringiensis subsp. wuhanensis, respectively. Therefore, the PCR-RFLP typing system is a facile method to detect both known and novel cry genes existing in B. thuringiensis strains.

Bacillus thuringiensis↗
PubMed2002

Presentation to accident and emergency with crying or screaming and likelihood of child protection registration.

OBJECTIVES: To determine whether children aged less than 2 years who present to accident and emergency (A&E) with crying or screaming as the only complaint, are more likely to be placed on the child protection register in later years than children who do not attend with crying or screaming alone. METHODS: The Sheffield Children's Hospital A&E database was examined for five years from 1 January 1992. Children who presented at triage with crying or screaming as their sole complaint were identified. Controls were taken from children who presented with any other complaint. Matches were made for sex, postcode and date of birth. All names were checked against against files that contained dates of past or present child protection registration. In January 2000, the children's age ranged from 3 to 10 years. The mean follow up period was six years (SD one year seven months). RESULTS: From 1 January 1992 until 31 December 1996, 450 children made 462 attendances to A&E with crying or screaming as their only complaint. Of these, 12 had been placed on the child protection register. Ten of the 450 control children had been registered. The odds ratio of subsequent child protection registration if a child presents in Sheffield with crying or screaming alone is 1.21 (95% confidence intervals 0.52 to 2.82). CONCLUSIONS: Presentation of young children who cry or scream for no clear reason is relatively common. Although child protection registration is not the same as abuse, it is the closest surrogate marker we have. This study shows there is no evidence of increased likelihood of child protection registration for children who present with crying or screaming alone and prejudices against parents of these children, if held, are inappropriate.

Child Abuse↗
PubMed2005

Determination of human linear IgE epitopes of Japanese cedar allergen Cry j 1.

Cry j 1 is one of the major allergens in Japanese cedar pollen. We attempt high throughput analysis and comprehensive identification of the linear IgE epitopes of Cry j 1. A series of overlapping synthetic Cry j 1 peptides chemically spotted on cellulose membrane was probed with sera from patients in Japan and United States, which were reactive to Cry j 1, and the reactivity of one of the detected sequences was confirmed by means of competitive ELISA using peptide as coated antigen. The peptide (331)NGNATPQLTKNA(342) (peptide 166) was detected by all three pooled sera used, and peptide (103)NGGPCVFIKRVS(114) (peptide 52) was detected by two of the three pools of sera. In addition, several peptides reacted with one of the pooled sera. IgE binding to peptide 166-coated wells was inhibited by addition of peptide 166 for several individual patient sera, suggesting that peptide 166 is one of the linear epitopes of Cry j 1. Since patients in United States were suggested to be rarely sensitized with Japanese cedar, they were sensitized with the similar tree pollen allergens such as Cup s 1 and Jun a 1, and cross-reacted with Cry j 1. We have comprehensively investigated human IgE epitopes of Cry j 1 and succeeded in identifying a common linear epitope, (331)NGNATPQLTKNA(342).

Allergens↗
PubMed2005

[Pain assessment using CRIES, FLACC and PIPP in high-risk infants].

PURPOSE: Infants at neonatal intensive care units (NICU) are invariably exposed to various procedural and environmental stimuli. The study was performed to compare the pain responses in three NICU stimulants and to examine the clinical feasibility for NICU infants using CRIES, FLACC and PIPP. METHOD: In a correlational study, a total of 94 NICU stimulants including angio-catheter insertions, trunk-rubbings and loud noises, was observed for pain responses among 64 infants using CRIES, FLACC and PIPP. RESULTS: A significant difference was identified among the mean scores in CRIES (F(2, 91)=47.847, p=.000), FLACC (F(2, 91)=41.249, p=.000) and PIPP (F(2, 91)=16.272, p=.000) to three stimulants. In a Post-hoc Scheff test, an angio-catheter insertion showed the highest scores in CRIES, FLACC and PIPP compared to the other two stimulations. A strong correlation was identified between CRIES and FLACC in all three stimulations (.817 < r < .945) while inconsistent findings were identified between PIPP and CRIES or FLACC. CONCLUSIONS: The results of the study support that CRIES and FLACC are reliable and clinically suitable pain measurements for NICU infants. Further studies are needed in data collection time-point as well as clinical feasibility on PIPP administration to assess pain response in infants, including premature infants.

Female↗
PubMed1995

[Production of a monoclonal antibody against Cry j 2].

The peptide possessing N-terminal 10 amino acid sequence of Cry j 2 was chemically synthesized and conjugated to KLH-carrier protein. Rabbits were immunized with this haptenized protein and the antibody (N-10 antibody) was obtained. Cross-blot analysis revealed that N-10 antibody recognized the hapten moiety. Purification of Cry j 2 was carried out with monitoring its reactivity with N-10 antibody. The substance reactive to N-10 antibody existed in the DEAE-Sephadex unadsorbed fraction. The substance reactive to N-10 antibody was subjected to CM-Sephadex equilibrated with 10 mM acetate buffer (pH 5.0). In contrast to the previous report on Cry j 2, the substance reactive to N-10 antibody existed in the CM-Sephadex unadsorbed fraction. The CM-Sephadex unadsorbed fraction was concentrated with 80% saturated ammonium sulfate, and applied to a Superdex pg 200 column. The major peak of protein was regarded as the final preparation and was subjected to SDS-PAGE. The substance reactive to N-10 antibody had a MW of 40 kDa under reducing and 37 kDa under non-reducing conditions, respectively. Furthermore, the substance reactive to N-10 antibody showed potent allergenic activity. These data completely agreed with the previous data on Cry j 2 and strongly suggested that the final preparation contained a large amount of Cry j 2. BALB/c mice were immunized intraperitoneally with the final preparation to produce a monoclonal antibody against Cry j 2. Eleven clones reacting to the final preparation were obtained, and the antibodies produced from these clones did not react with purified Cry j 1.(ABSTRACT TRUNCATED AT 250 WORDS)

Allergens↗
PubMed1995

Infantile colic. Seasonal incidence and crying profiles.

OBJECTIVES: To determine the occurrence of infantile colic and its seasonal variation in an unselected population and to evaluate the amounts of crying in colicky infants and noncolicky controls. DESIGN: Questionnaire survey and a prospective substudy of parental diaries of crying. SETTING: All families with a full-term, healthy-born infant in the Turku City (Finland) district during 1 year. PARTICIPANTS: Colic was defined as paroxysms of crying for 3 or more hours per day for 3 days or more per week during a period of at least 3 weeks. The questionnaires containing this definition were distributed to 1221 families in postpartum wards, and an invitation to a prospective follow-up study was presented to the families if their infant showed colicky symptoms. The incidence questionnaire was to be returned after 3 months. Six hundred four (49%) of the questionnaires were returned, and an additional 355 (29%) families were reached by phone. A total of 59 families with a colicky infant enrolled in the prospective substudy when the infants were at a median age of 5 weeks; age-matched controls were invited from the same population. RESULTS: The incidence of infantile colic was 13%; possible infantile colic was 8%. No seasonal variation was found. The mean amount of total crying was 241 min/d (95% confidence interval [CI], 216 to 266 minutes) in the colic group and 112 min/d (95% CI, 95 to 130 minutes) in the control group during the first recording week. The mean amount of colicky crying was 122 min/d (95% CI, 102 to 142 minutes) in the colic group and 19 min/d (95% CI, 12 to 26 minutes) in the control group. CONCLUSIONS: The incidence of colic was 13% with no seasonal variation. Parental perception of infantile colic correlated well with the amount of crying.

Colic↗
PubMed1999

Feasibility of using fMRI to study mothers responding to infant cries.

While parenting is a universal human behavior, its neuroanatomic basis is currently unknown. Animal data suggest that the cingulate may play an important function in mammalian parenting behavior. For example, in rodents cingulate lesions impair maternal behavior. Here, in an attempt to understand the brain basis of human maternal behavior, we had mothers listen to recorded infant cries and white noise control sounds while they underwent functional MRI (fMRI) of the brain. We hypothesized that mothers would show significantly greater cingulate activity during the cries compared to the control sounds. Of 7 subjects scanned, 4 had fMRI data suitable for analysis. When fMRI data were averaged for these 4 subjects, the anterior cingulate and right medial prefrontal cortex were the only brain regions showing statistically increased activity with the cries compared to white noise control sounds (cluster analysis with one-tailed z-map threshold of P < 0.001 and spatial extent threshold of P < 0.05). These results demonstrate the feasibility of using fMRI to study brain activity in mothers listening to infant cries and that the anterior cingulate may be involved in mothers listening to crying babies. We are currently replicating this study in a larger group of mothers. Future work in this area may help (1) unravel the functional neuroanatomy of the parent-infant bond and (2) examine whether markers of this bond, such as maternal brain response to infant crying, can predict maternal style (i.e., child neglect), offspring temperament, or offspring depression or anxiety.

Adult↗
PubMed2003

Changing determinants of crying termination in 6- to 12-week-old human infants.

Developmental change in human infant crying termination was studied in 60 infants who were 6, 9, and 12 weeks of age. They qualified by spontaneously crying for at least 30 s during the study's first minute. Infants then received either 0.8 ml sucrose (12% w/v) by syringe or sucked a pacifier dipped in the 12% solution for a total of 3.5 min. Crying was substantially reduced by pacifier-sucking at all three ages tested. Sucrose too was effective at 6 and 9 weeks of age but did not reduce crying in 12-week-olds. These data suggest different schedules in the decline of sweet taste and pacifier sucking as agents of crying termination. These differential rates argue for multiple systems that govern crying reduction and argue against a single central target that changes ontogenetically in sensitivity and control. Whether the continuation of sucking effectiveness is sustained by infant sucking experience or reflects a slower rate of decline cannot be determined at present.

Crying↗
PubMed1983

Individual recognition of human infants on the basis of cries alone.

Human parents were asked to identify their infants on the basis of tape-recorded cries that they had not previously heard. The cries of twenty 30-day-old infants were recorded just prior to a feeding, then rerecorded onto a test tape containing cries from three other infants. Eighty percent of mothers were able to recognize their infants' cries, as were 45% of fathers. An additional 140 adults (non-parents) were tested in order to determine if the process of dubbing cries onto test tapes had left extraneous auditory cues to infants' identities and if the foil infants were equally discriminable. The results indicated that parents' recognition was not based on extraneous cues and that, overall, the foils were appropriate distractors in the parents' task. Thus, the majority of parents can recognize their 30-day-old infants on the sole basis of acoustic cues contained in the infants' cries. The acoustic features that underlie this recognition are now being investigated.

Auditory Perception↗
PubMed1993

Rhythmic organization of the sound of infant crying.

The purpose of the present study was to examine the temporal morphology and rhythmic structure underlying the repeated bursts of expiratory sounds in a sustained bout of crying of twenty-three 1-month-old infants. Durations of expiratory sounds and bursts were determined from a 90-s bout of naturally occurring cries recorded in the home before feeding. Results indicated wide individual differences in temporal morphology between infants and within infant cry sounds. Binary spectrum analysis of the presence of expiratory sounds in the cry sound detected rhythms in temporal organization at a wide range of dominant frequencies. Spectral complexity (higher numbers of peaks in the power spectrum) was related to a longer time since infants were last fed and shorter expiratory sounds. Results emphasize the importance of viewing cries of young infants as dynamic signals. An ontogenetic history of the rhythms of infant cry sounds may contribute to understanding organismic and environmental experiences which contribute to development.

Child Behavior↗
PubMed1994

Robustness of individual identity in the cries of human infants.

This study investigated the possibility that one function of the human infant's cry is to convey individual identity across distance. Six-hundred adults, having been exposed to 30 s of an infant's crying, were asked to identify this infant on the basis of other, experimentally altered, cries. The cries were altered naturally by rerecording across distance in the out-of-doors, and artificially by bandpass filtering and temporal reorganization. The individuality of cries proved remarkably robust to degradation: Only when frequencies were limited to the range of 8 to 10 kHz was recognition performance significantly impaired. It is argued that the human infant's cry, a complex signal with multiple markers of individuality, may have among its functions the communication of infants' identities across distance.

Adolescent↗
PubMed1978

Cry analysis in infants with severe malnutrition.

Sound spectrographic investigations of the cries of 5 infants, age 7 m to 2 y, with severe malnutrition (one with kwashiorkor and four with marasmus) were compared with the cries of 15 healthy children of corresponding age. The cry of the child with kwashiorkor resembled those of the normal infants. The cries of the marasmic children showed a significant increase in the minimum and maximum pitch, and the occurrence of biphonation and flat melody types. These features have also been found in the cries of children with brain damage. We therefore believe that cry and analysis can be an additional means of investigating to what degree the brain is affected in children with malnutrition.

Child, Preschool↗
PubMed1993

Citalopram for post-stroke pathological crying.

Post-stroke pathological crying is a distressing condition in which episodes occur in response to minor stimuli without associated mood changes. There is preliminary evidence of disturbed serotoninergic neurotransmission in such cases. We investigated the effect of the selective serotonin reuptake inhibitor citalopram on uncontrolled crying in stroke patients in a double-blind placebo-controlled crossover study. 16 consecutive patients (median age 58.5 years, range 40-83) entered the 9-week study a median of 168 days (range 6-913) post stroke and were treated with citalopram 10-20 mg daily for 3 weeks. Crying history was determined from semistructured interviews and from diaries kept by the patients. Psychiatric assessment was made with the Hamilton depression scale (HDS), and unwanted effects were measured with the UKU side-effect scale. In 13 patients in whom frequency of crying could be assessed, the number of daily crying episodes decreased by at least 50% in all cases during citalopram treatment vs 2 patients during placebo treatment (p < 0.005, McNemar's test), the effect being rapid (1-3 days) and pronounced in 11 (73%). There was a concomitant significant decrease in depression rating from HDS 8.9 to 5.3 (p < 0.005, Wilcoxon's test). Citalopram was well tolerated, the few side-effects being mild and transient. We conclude that serotoninergic neurotransmission plays an important part in post-stroke pathological crying and that citalopram is an effective and well-tolerated treatment.

Adult↗
PubMed1987

Pain expression in neonates: facial action and cry.

Pain expression in neonates instigated by heel-lance for blood sampling purposes was systematically described using measures of facial expression and cry and compared across sleep/waking states and sex. From gate-control theory it was hypothesized that pain behavior would vary with the ongoing functional state of the infant, rather than solely reflecting tissue insult. Awake-alert but inactive infants responded with the most facial activity, consistent with current views that infants in this state are most receptive to environmental stimulation. Infants in quiet sleep showed the least facial reaction and the longest latency to cry. Fundamental frequency of cry was not related to sleep/waking state. This suggested that findings from the cry literature on qualities of pain cry as a reflection of nervous system 'stress', in unwell newborns, do not generalize directly to healthy infants as a function of state. Sex differences were apparent in speed of response, with boys showing shorter time to cry and to display facial action following heel-lance. The findings of facial action variation across sleep/waking state were interpreted as indicating that the biological and behavioral context of pain events affects behavioral expression, even at the earliest time developmentally, before the opportunity for learned response patterns occurs. Issues raised by the study include the importance of using measurement techniques which are independent of preconceived categories of affective response.

Crying↗
PubMed2006

Longitudinal study of the fundamental frequency of hunger cries along the first 6 months of healthy babies.

SUMMARY: Potentially rich in information, the baby's cry has motivated several researches along the years. Although most of these studies have generated important knowledge about the baby's cry, they were focused on the neonatal period. The few longitudinal studies on changes in the acoustical features of the cry over the baby's growth have been done with a small sample and a large recording interval. Aiming to overcome such methodological limitations, this work investigated hunger cries using a more representative sample size (30 babies) and time resolution (biweekly intervals) from birth to 6 months of baby's age. The findings indicate that the fundamental frequency (f0) of the cry signals did vary more than previously reported in the literature. The results showed a widespread oscillatory behavior in f0 evolution along all the 6 months with an especially significant decrease from birth to the 15th day of life. The present results are not clinically applicable yet, but they pointed some novel aspects of the f0 mean values along the baby's growth. These findings and further longitudinal studies can help standardize age-related cry parameters, which are essential for medical and language development researches.

Age Factors↗
PubMed2005

Do pregnancy and childbirth adversities predict infant crying and colic? Findings and recommendations.

Bouts of unexplained crying in 1- to 3-month-old infants are a common problem for parents and health services. One proposed explanation has linked the crying to preceding adversities, such as maternal stress and cigarette smoking during pregnancy and complications during childbirth. In the first part of this review, we argue that studies of these links have methodological shortcomings, and make recommendations about the safeguards needed to overcome these shortcomings. In part two, we present a study that assesses the relations between adversity indices and validated measures of crying in two separate cohorts of infants. Four indices of childbirth adversity predicted infant crying separately and cumulatively in cohort 1, but not in cohort 2. We conclude that there is a need for further research that includes replication and other safeguards. Infant crying is a highly emotional issue for many parents. Before researchers add to their burden by claiming that maternal prenatal anxiety, cigarette smoking, or labour medication, contribute to their baby's crying, we need to be sure of our grounds.

Adult↗
PubMed2000

When nurses cry: coping with occupational stress in Thailand.

Anecdotal reports of people feeling better after they cry support theories that link crying to the reduction of stress after a period of prolonged sympathetic activation. A sample of 200 nurses were asked to rate their occupational stress, job satisfaction, and crying as a coping strategy. Crying was found to be an important symptom of home/work conflicts and pressures related to dealing with patients, but did not substantially reduce these sources of stress. Supporting the stress-buffering hypothesis, nurses with lower intrinsic job satisfaction seemed to benefit from emotional crying whereas dissatisfied nurses who cry infrequently reported the highest levels of stress.

Adaptation, Psychological↗